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ExperimentTesting newly discovered Biggie Phage editors in human cells
Experiment ConditionCas12j-GFP6 (CasΦ-3)
Assay Description: A plasmid expressing CasΦ and gRNA tranfected into stable GFP cell line in triplciate. 72 hours post transfection, GFP negative cells were quantified by flow cytometry
Tissue Measured:
Measurement Type:Editing Efficiency
Record ID15000000053

EditorCasΦ-3
Delivery SystemLipofectamine 3000
Delivery System SubtypeCommercial Reagent
GuideCas12j-GFP6 (CasΦ-3)
VectorpPP444;

Model SpeciesHuman
Model NameHEK293-TgEF1a-eGFP-BSD

Application Methodlipid transfection, after 24 hours add Puromycin selection
Application Site
Dosage200 ng
Injection Frequency
Injection Rate
Injection Volume
Days post injection72 hours
Editor Formatplasmid
Antidote Id
Antidote Description

Measured Values
Samples Size: 3
 
Replicate Result (Editing Efficiency)
Measurement Units: % of GFP negative cells
Mean 1.37 
1 1.6400000000000001 
2 1.21 
3 1.27 
  

Publication Title
CRISPR-CasΦ from huge phages is a hypercompact genome editor. NCBI