Testing newly discovered Biggie Phage editors in human cells |
Cas12j-GFP14 (CasΦ-3) |
A plasmid expressing CasΦ and gRNA tranfected into stable GFP cell line in triplciate. 72 hours post transfection, GFP negative cells were quantified by flow cytometry |
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Editing Efficiency |
15000000061 |
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CasΦ-3 |
Lipofectamine 3000 |
Commercial Reagent |
Cas12j-GFP14 (CasΦ-3) |
pPP444; |
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Human |
HEK293-TgEF1a-eGFP-BSD |
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lipid transfection, after 24 hours add Puromycin selection |
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200 ng |
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72 hours |
plasmid |
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Measured Values |
Samples Size: | 3 |
|
Mean |
0.79  |
1 |
0.8099999999999999  |
2 |
0.8200000000000001  |
3 |
0.75  |
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