Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells |
FS66d6 |
Calculated editing efficiency by % of NGS reads that showed an indel |
|
Editing Efficiency |
15000000705 |
|
SpCas9 (Feldan Therapeutics) |
FS66d6 |
Amphiphilic peptide |
McCray_CFTR_Cas9_Guide1 |
|
|
Human |
Primary Airway Epithelia |
|
|
|
2uM gRNA, 1.33uM Cas12a, 40uM Shuttle |
|
|
|
|
RNP |
|
|
|
Measured Values |
Samples Size: | 11 |
|
Mean |
1.79  |
1 |
7.77  |
2 |
6.28  |
3 |
0.24  |
4 |
0.8  |
5 |
0.44  |
6 |
0.6  |
7 |
2.37  |
8 |
1.16  |
9 |
0.0245  |
10 |
0.0053  |
11 |
0.00485  |
| |
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