Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells |
FSD112 |
Calculated editing efficiency by % of NGS reads that showed an indel |
|
Editing Efficiency |
15000000706 |
|
SpCas9 (Feldan Therapeutics) |
FSD112 |
Amphiphilic peptide |
McCray_CFTR_Cas9_Guide1 |
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|
Human |
Primary Airway Epithelia |
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|
2uM gRNA, 1.33uM Cas12a, 40uM Shuttle |
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RNP |
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Measured Values |
Samples Size: | 14 |
|
Mean |
1.47  |
1 |
5.6  |
2 |
3.27  |
3 |
1.44  |
4 |
1.5  |
5 |
0.48  |
6 |
1.32  |
7 |
1.34  |
8 |
1.26  |
9 |
3.49  |
10 |
0.62  |
11 |
0.14  |
12 |
0.07425  |
13 |
0.0531  |
14 |
0.05345  |
| |
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