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ExperimentPeptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells
Experiment ConditionFSD168d10
Assay Description: Calculated editing efficiency by % of NGS reads that showed an indel
Tissue Measured:
Measurement Type:Editing Efficiency
Record ID15000000724

EditorSpCas9 (Feldan Therapeutics)
Delivery SystemFSD168d10
Delivery System SubtypeAmphiphilic peptide
GuideMcCray_CFTR_Cas9_Guide1
Vector

Model SpeciesHuman
Model NamePrimary Airway Epithelia

Application Method
Application Site
Dosage2uM gRNA, 1.33uM Cas12a, 40uM Shuttle
Injection Frequency
Injection Rate
Injection Volume
Days post injection
Editor FormatRNP
Antidote Id
Antidote Description

Measured Values
Samples Size: 3
 
Replicate Result (Editing Efficiency)
Measurement Units: % of reads
Mean 0.82 
1 0.72 
2 0.97 
3 0.78