Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells |
FSD168d17 |
Calculated editing efficiency by % of NGS reads that showed an indel |
|
Editing Efficiency |
15000000727 |
|
SpCas9 (Feldan Therapeutics) |
FSD168d17 |
Amphiphilic peptide |
McCray_CFTR_Cas9_Guide1 |
|
|
Human |
Primary Airway Epithelia |
|
|
|
2uM gRNA, 1.33uM Cas12a, 40uM Shuttle |
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|
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|
RNP |
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|
Measured Values |
Samples Size: | 6 |
|
Mean |
5.9  |
1 |
6.19  |
2 |
11.94  |
3 |
9.32  |
4 |
2.35  |
5 |
2.3  |
6 |
3.27  |
| |
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