Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells |
FSD238 |
Calculated editing efficiency by % of NGS reads that showed an indel |
|
Editing Efficiency |
15000000748 |
|
SpCas9 (Feldan Therapeutics) |
FSD238 |
Amphiphilic peptide |
McCray_CFTR_Cas9_Guide1 |
|
|
Human |
Primary Airway Epithelia |
|
|
|
2uM gRNA, 1.33uM Cas12a, 40uM Shuttle |
|
|
|
|
RNP |
|
|
|
Measured Values |
Samples Size: | 11 |
|
Mean |
7.43  |
1 |
13.54  |
2 |
13.45  |
3 |
16.86  |
4 |
2.72  |
5 |
6.96  |
6 |
1.95  |
7 |
8.1  |
8 |
5.1  |
9 |
2.13  |
10 |
7.56  |
11 |
3.38  |
| |
|