Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells |
S10 |
Calculated editing efficiency by % of NGS reads that showed an indel |
|
Editing Efficiency |
15000000821 |
|
SpCas9 (Feldan Therapeutics) |
S10 |
Amphiphilic peptide |
McCray_CFTR_Cas9_Guide1 |
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|
Human |
Primary Airway Epithelia |
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2uM gRNA, 1.33uM Cas12a, 40uM Shuttle |
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RNP |
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Measured Values |
Samples Size: | 22 |
|
Mean |
2.76  |
1 |
1.82  |
2 |
2.51  |
3 |
0.83  |
4 |
0.74  |
5 |
3.87  |
6 |
0.8  |
7 |
3.06  |
8 |
0.72  |
9 |
2.47  |
10 |
0.5  |
11 |
1.17  |
12 |
3.71  |
13 |
2.67  |
14 |
3.88  |
15 |
6.47  |
16 |
3.58  |
17 |
1.70999997854232  |
18 |
3.9300000667572  |
19 |
4.58499991893768  |
20 |
2.25  |
21 |
6.11  |
22 |
3.27  |
| |
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