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ExperimentPeptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
Experiment ConditionFSD238
Assay Description: Calculated editing efficiency by % of NGS reads that showed an indel
Tissue Measured:
Measurement Type:Editing Efficiency
Record ID15000000868

EditorAsCas12a (Feldan Therapeutics)
Delivery SystemFSD238
Delivery System SubtypeAmphiphilic peptide
Guide3849 45-5'
Vector

Model SpeciesHuman
Model NamePrimary Airway Epithelia

Application Method
Application Site
Dosage2uM gRNA, 1.33uM Cas12a, 40uM Shuttle
Injection Frequency
Injection Rate
Injection Volume
Days post injection
Editor Formatribonucleoprotein (RNP)
Antidote Id
Antidote Description

Measured Values
Samples Size: 3
 
Replicate Result (Editing Efficiency)
Measurement Units: % of reads
Mean 24.49 
1 24.6 
2 20.91 
3 27.95