Experiment A novel human T cell platform to define biological adverse effects of genome editing Experiment Condition LAG3_site_05 Assay Description: Calculated editing efficiency by targeted amplicon deep sequencing Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000000490 Editor SpCas9 Delivery System P3 Nucleofection Kit Delivery System Subtype Commercial Reagent Guide LAG3_site_05 Vector Model Species Human Model Name CD4/CD8 Human Primary T cell Application Method nucleofection Application Site Dosage 75 pmol Cas9 protein; 3-fold molar excess of sgRNA Injection Frequency Injection Rate Injection Volume Days post injection Editor Format RNP Antidote Id Antidote Description Measured Values Samples Size: 3   Replicate Result (Editing Efficiency) Measurement Units: % of reads Mean 19.67  1 20.0051604912788  2 19.2077244708824  3 19.7999141348083