Experiment A novel human T cell platform to define biological adverse effects of genome editing Experiment Condition PTPN2_site_03 Assay Description: Calculated editing efficiency by targeted amplicon deep sequencing Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000000514 Editor SpCas9 Delivery System P3 Nucleofection Kit Delivery System Subtype Commercial Reagent Guide PTPN2_site_03 Vector Model Species Human Model Name CD4/CD8 Human Primary T cell Application Method nucleofection Application Site Dosage 75 pmol Cas9 protein; 3-fold molar excess of sgRNA Injection Frequency Injection Rate Injection Volume Days post injection Editor Format RNP Antidote Id Antidote Description Measured Values Samples Size: 3   Replicate Result (Editing Efficiency) Measurement Units: % of reads Mean 65.92  1 66.98215909527529  2 66.52532459936839  3 64.2576630795124