Experiment Testing newly chemically modified crRNA and tracrRNA to activate the TLR reporter in human cells Experiment Condition STS118 (TLR-MCV1a) (20 pmol RNP) Assay Description: Calculated editing efficiency by flow cytometry analysis of RFP expression. Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000000548 Editor SpyCas9-3xNLS Delivery System FSD117D1 Delivery System Subtype Amphiphilic peptide Guide STS118 (TLR-MCV1a; Sp_t2:Sp_c20_TLR_MCV1a) Vector Model Species Human Model Name HEK-293T-disrupted_GFP with MCV-mcherry-Puro Application Method electroporation Application Site Dosage 20 pmol RNP Injection Frequency Injection Rate Injection Volume Days post injection 48-72 hours Editor Format protein, RNA Antidote Id Antidote Description Measured Values Samples Size: 3   Replicate Result (Editing Efficiency) Measurement Units: % of RFP+ cells Mean 24.8  1 25.6  2 24.3  3 24.5