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ExperimentTesting newly chemically modified crRNA and tracrRNA in mTmG mouse embryonic fibroblasts
Experiment ConditionSTS159 (mTmG) (2 pmol RNP)
Assay Description: Calculated editing efficiency by flow cytometry analysis of GFP and RFP.
Tissue Measured:
Measurement Type:Editing Efficiency
Record ID15000000550

EditorSpyCas9-3xNLS
Delivery System
Delivery System Subtype
GuideSTS159 (mTmG; Sp_t2:Sp_c20_mTmG)
Vector

Model SpeciesMouse
Model NameMEF

Application Methodelectroporation
Application Site
Dosage2 pmol RNP
Injection Frequency
Injection Rate
Injection Volume
Days post injection48-72 hours
Editor Formatprotein, RNA
Antidote Id
Antidote Description

Measured Values
Samples Size: 2
 
Replicate Result (Editing Efficiency)
Measurement Units: % of GFP+ cells
Mean 24.2 
1 24.8 
2 23.6