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ExperimentTesting newly chemically modified crRNA and tracrRNA to activate the TLR1 reporter in human cells
Experiment ConditionSTS120 (TLR1) (20 pmol RNP)
Assay Description: Calculated editing efficiency by flow cytometry analysis of RFP.
Tissue Measured:
Measurement Type:Editing Efficiency
Record ID15000000553

EditorSpyCas9-3xNLS
Delivery System
Delivery System Subtype
GuideSTS120 (TLR1; Sp_t2:Sp_c20_TLR1)
Vector

Model SpeciesHuman
Model NameHEK-293T-disrupted_GFP-mcherry-Puro

Application Methodelectroporation
Application Site
Dosage20 pmol RNP
Injection Frequency
Injection Rate
Injection Volume
Days post injection48-72 hours
Editor Formatprotein, RNA
Antidote Id
Antidote Description

Measured Values
Samples Size: 2
 
Replicate Result (Editing Efficiency)
Measurement Units: % of RFP+ cells
Mean 29.0 
1 30 
2 28