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ExperimentAmphiphilic Peptides Deliver Base Editor RNPs to Rhesus Monkey Airway
Experiment ConditionS10 and ABE8e-Cas9 RNP
Assay Description: Editing efficiency was assessed using Next Generation Sequencing of airway epithelial cells obtained by airway brushing in two airways of the airway tree
Tissue Measured:left main bronchus
Cell Type :bronchial epithelial cell
Measurement Type:Editing Efficiency
Record ID15000002991

EditorABE8e-Cas9
Delivery SystemS10
Delivery System SubtypeAmphiphilic peptide
GuidegRNA #8
Vector

Model SpeciesRhesus macaque
Model NameMacaca mulatta (Rhesus Macaque)

Application Methodintra-tracheal instillation
Application Siteairway epithelium
Dosage2 uM gRNA, 2.5 uM Cas9, 40 uM S10 amphiphilic peptide
Injection Frequencyonce
Injection Raten/a
Injection Volume1 ml
Days post injection7 days
Editor Formatribonucleoprotein (RNP)
Antidote Id
Antidote Description

Measured Values
Samples Size: 1
 
Replicate Result (Editing Efficiency)
Measurement Units: % of A•T basepairs converted to G•C
Mean 0.03 
1 0.032