LOCUS addgene_plasmid_ 5342 bp ds-DNA circular 15-NOV-2019 DEFINITION AAV expression of Cre from CMV promoter. ACCESSION . VERSION . KEYWORDS pENN.AAV.CMVs.Pl.Cre.rBG SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5342) TITLE AAV plasmids REFERENCE 2 (bases 1 to 5342) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers repeat_region 1..130 /locus_tag="AAV2 ITR" /label="AAV2 ITR" /ApEinfo_label="AAV2 ITR" /ApEinfo_fwdcolor="#0dfff7" /ApEinfo_revcolor="#0dfff7" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 485..688 /note="human cytomegalovirus (CMV) immediate early promoter" /locus_tag="CMV promoter" /label="CMV promoter" /ApEinfo_label="CMV promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 639..659 /note="Human CMV immediate early promoter, forward primer" /locus_tag="CMV-F" /label="CMV-F" /ApEinfo_label="CMV-F" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 685..709 /note="Human CMV promoter, forward primer" /locus_tag="LNCX" /label="LNCX" /ApEinfo_label="LNCX" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" intron 824..956 /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" /locus_tag="chimeric intron" /label="chimeric intron" /ApEinfo_label="chimeric intron" /ApEinfo_fwdcolor="#eb606c" /ApEinfo_revcolor="#eb606c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 939..958 /note="Synthetic intron, forward primer" /locus_tag="pMT2-F" /label="pMT2-F" /ApEinfo_label="pMT2-F" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 1001..1019 /note="promoter for bacteriophage T7 RNA polymerase" /locus_tag="T7 promoter" /label="T7 promoter" /ApEinfo_label="T7 promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 1070..2101 /codon_start=1 /product="site-specific recombinase" /note="Cre is a site-specific recombinase from bacteriophage P1. Recombination occurs at loxP sequences." /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKM L LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSG L PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLA F LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLV E RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSG Q RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLL E DGD" /locus_tag="Cre" /label="Cre" /ApEinfo_label="Cre" /ApEinfo_fwdcolor="#e9d024" /ApEinfo_revcolor="#e9d024" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(2179..2198) /note="Rabbit beta-globin polyA region, reverse primer" /locus_tag="Bglob-pA-R" /label="Bglob-pA-R" /ApEinfo_label="Bglob-pA-R" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" polyA_site 2244..2299 /note="rabbit beta-globin polyadenylation signal" /locus_tag="-beta-globin poly(A) signal" /label="-beta-globin poly(A) signal" /ApEinfo_label="-beta-globin poly(A) signal" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" repeat_region 2392..2521 /locus_tag="AAV2 ITR(1)" /label="AAV2 ITR(1)" /ApEinfo_label="AAV2 ITR" /ApEinfo_fwdcolor="#0dfff7" /ApEinfo_revcolor="#0dfff7" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(2540..2557) /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" /locus_tag="M13 Forward" /label="M13 Forward" /ApEinfo_label="M13 Forward" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(2540..2556) /note="common sequencing primer, one of multiple similar variants" /locus_tag="M13 fwd" /label="M13 fwd" /ApEinfo_label="M13 fwd" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(2549..2571) /note="In lacZ gene" /locus_tag="M13/pUC Forward" /label="M13/pUC Forward" /ApEinfo_label="M13/pUC Forward" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin 2698..3153 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /locus_tag="f1 ori" /label="f1 ori" /ApEinfo_label="f1 ori" /ApEinfo_fwdcolor="#999999" /ApEinfo_revcolor="#999999" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(2785..2804) /note="F1 origin, reverse primer" /locus_tag="F1ori-R" /label="F1ori-R" /ApEinfo_label="F1ori-R" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 2995..3016 /note="F1 origin, forward primer" /locus_tag="F1ori-F" /label="F1ori-F" /ApEinfo_label="F1ori-F" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 3179..3283 /gene="bla" /locus_tag="AmpR promoter" /label="AmpR promoter" /ApEinfo_label="AmpR promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 3284..4144 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY I ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY S PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR W EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS A LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA S LIKHW" /locus_tag="AmpR" /label="AmpR" /ApEinfo_label="AmpR" /ApEinfo_fwdcolor="#e9d024" /ApEinfo_revcolor="#e9d024" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(3502..3521) /note="Ampicillin resistance gene, reverse primer" /locus_tag="Amp-R" /label="Amp-R" /ApEinfo_label="Amp-R" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin 4315..4903 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /locus_tag="ori" /label="ori" /ApEinfo_label="ori" /ApEinfo_fwdcolor="#999999" /ApEinfo_revcolor="#999999" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 4804..4823 /note="pBR322 origin, forward primer" /locus_tag="pBR322ori-F" /label="pBR322ori-F" /ApEinfo_label="pBR322ori-F" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 5057..5074 /note="L4440 vector, forward primer" /locus_tag="L4440" /label="L4440" /ApEinfo_label="L4440" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" protein_bind 5191..5212 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /locus_tag="CAP binding site" /label="CAP binding site" /ApEinfo_label="CAP binding site" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 5227..5257 /note="promoter for the E. coli lac operon" /locus_tag="lac promoter" /label="lac promoter" /ApEinfo_label="lac promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" protein_bind 5265..5281 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /locus_tag="lac operator" /label="lac operator" /ApEinfo_label="lac operator" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 5270..5292 /note="In lacZ gene" /locus_tag="M13/pUC Reverse" /label="M13/pUC Reverse" /ApEinfo_label="M13/pUC Reverse" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 5289..5305 /note="common sequencing primer, one of multiple similar variants" /locus_tag="M13 rev" /label="M13 rev" /ApEinfo_label="M13 rev" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 5289..5305 /note="In lacZ gene. Also called M13-rev" /locus_tag="M13 Reverse" /label="M13 Reverse" /ApEinfo_label="M13 Reverse" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 CTGCGCGCTC GCTCGCTCAC TGAGGCCGCC CGGGCAAAGC CCGGGCGTCG GGCGACCTTT 61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT 121 AGGGGTTCCT TGTAGTTAAT GATTAACCCG CCATGCTACT TATCTACCAG GGTAATGGGG 181 ATCCTCTAGA ACTATAGCTA GCATGCCTGC AGGCTGACCG CCCAACGACC CCCGCCCATT 241 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA 301 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC 361 AAGTCCGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA 421 CATGACCTTA CGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC 481 CATGGTGATG CGGTTTTGGC AGTACACCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG 541 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG 601 GGACTTTCCA AAATGTCGTA ATAACCCCGC CCCGTTGACG CAAATGGGCG GTAGGCGTGT 661 ACGGTGGGAG GTCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCA CTAGAAGCTT 721 TATTGCGGTA GTTTATCACA GTTAAATTGC TAACGCAGTC AGTGCTTCTG ACACAACAGT 781 CTCGAACTTA AGCTGCAGAA GTTGGTCGTG AGGCACTGGG CAGGTAAGTA TCAAGGTTAC 841 AAGACAGGTT TAAGGAGACC AATAGAAACT GGGCTTGTCG AGACAGAGAA GACTCTTGCG 901 TTTCTGATAG GCACCTATTG GTCTTACTGA CATCCACTTT GCCTTTCTCT CCACAGGTGT 961 CCACTCCCAG TTCAATTACA GCTCTTAAGG CTAGAGTACT TAATACGACT CACTATAGGC 1021 TAGCCTCGAG AATTGTACAC TTTACTTAAA ACCATTATCT GAGTGTGAAA TGTCCAATTT 1081 ACTGACCGTA CACCAAAATT TGCCTGCATT ACCGGTCGAT GCAACGAGTG ATGAGGTTCG 1141 CAAGAACCTG ATGGACATGT TCAGGGATCG CCAGGCGTTT TCTGAGCATA CCTGGAAAAT 1201 GCTTCTGTCC GTTTGCCGGT CGTGGGCGGC ATGGTGCAAG TTGAATAACC GGAAATGGTT 1261 TCCCGCAGAA CCTGAAGATG TTCGCGATTA TCTTCTATAT CTTCAGGCGC GCGGTCTGGC 1321 AGTAAAAACT ATCCAGCAAC ATTTGGGCCA GCTAAACATG CTTCATCGTC GGTCCGGGCT 1381 GCCACGACCA AGTGACAGCA ATGCTGTTTC ACTGGTTATG CGGCGGATCC GAAAAGAAAA 1441 CGTTGATGCC GGTGAACGTG CAAAACAGGC TCTAGCGTTC GAACGCACTG ATTTCGACCA 1501 GGTTCGTTCA CTCATGGAAA ATAGCGATCG CTGCCAGGAT ATACGTAATC TGGCATTTCT 1561 GGGGATTGCT TATAACACCC TGTTACGTAT AGCCGAAATT GCCAGGATCA GGGTTAAAGA 1621 TATCTCACGT ACTGACGGTG GGAGAATGTT AATCCATATT GGCAGAACGA AAACGCTGGT 1681 TAGCACCGCA GGTGTAGAGA AGGCACTTAG CCTGGGGGTA ACTAAACTGG TCGAGCGATG 1741 GATTTCCGTC TCTGGTGTAG CTGATGATCC GAATAACTAC CTGTTTTGCC GGGTCAGAAA 1801 AAATGGTGTT GCCGCGCCAT CTGCCACCAG CCAGCTATCA ACTCGCGCCC TGGAAGGGAT 1861 TTTTGAAGCA ACTCATCGAT TGATTTACGG CGCTAAGGAT GACTCTGGTC AGAGATACCT 1921 GGCCTGGTCT GGACACAGTG CCCGTGTCGG AGCCGCGCGA GATATGGCCC GCGCTGGAGT 1981 TTCAATACCG GAGATCATGC AAGCTGGTGG CTGGACCAAT GTAAATATTG TCATGAACTA 2041 TATCCGTAAC CTGGATAGTG AAACAGGGGC AATGGTGCGC CTGCTGGAAG ATGGCGATTA 2101 GCCATTAACG CGGCGTGGTA CCTCTAGAGT CGACCCGGGC GGCCTCGAGG ACGGGGTGAA 2161 CTACGCCTGA GGATCCGATC TTTTTCCCTC TGCCAAAAAT TATGGGGACA TCATGAAGCC 2221 CCTTGAGCAT CTGACTTCTG GCTAATAAAG GAAATTTATT TTCATTGCAA TAGTGTGTTG 2281 GAATTTTTTG TGTCTCTCAC TCGGAAGCAA TTCGTTGATC TGAATTTCGA CCACCCATAA 2341 TACCCATTAC CCTGGTAGAT AAGTAGCATG GCGGGTTAAT CATTAACTAC AAGGAACCCC 2401 TAGTGATGGA GTTGGCCACT CCCTCTCTGC GCGCTCGCTC GCTCACTGAG GCCGGGCGAC 2461 CAAAGGTCGC CCGACGCCCG GGCTTTGCCC GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA 2521 GCCTTAATTA ACCTAATTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG 2581 GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG 2641 AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGGACG 2701 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 2761 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 2821 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 2881 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 2941 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 3001 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 3061 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 3121 CGAATTTTAA CAAAATATTA ACGCTTACAA TTTAGGTGGC ACTTTTCGGG GAAATGTGCG 3181 CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA 3241 ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT 3301 CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA 3361 AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA 3421 ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT 3481 GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA 3541 AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT 3601 CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC 3661 CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT 3721 AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA 3781 GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC 3841 AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT 3901 AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG 3961 CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC 4021 ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC 4081 AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG 4141 GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA 4201 ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG 4261 TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA 4321 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT 4381 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG 4441 AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA 4501 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG 4561 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA 4621 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC 4681 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA 4741 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC 4801 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG 4861 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC 4921 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC 4981 CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG 5041 CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA 5101 ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA 5161 CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC 5221 CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA 5281 ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAGA TTTAATTAAG GCCTTAATTA 5341 GG //