LOCUS pTR_scU6-DMD 5867 bp ds-DNA circular 20-MAY-2021 DEFINITION . FEATURES Location/Qualifiers misc_feature 1..115 /label="ITR1" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 121..365 /label="U6-1" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 367..386 /label="DCR40" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 387..469 /label="chimericgrna1" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer_bind complement(502..521) /label="T3" /ApEinfo_revcolor=#008b8b /ApEinfo_fwdcolor=#008b8b misc_feature 528..772 /label="U6-2" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 774..793 /label="DCR112" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 794..876 /label="chimericgRNA-2" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer_bind complement(909..928) /label="T3" /ApEinfo_revcolor=#008b8b /ApEinfo_fwdcolor=#008b8b misc_feature 1150..1869 /label="EGFP" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 1887..2144 /label="BGH+PolyA" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e LTR 2151..2280 /label="ITR2" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd CDS 2282..2350 /label="LacZ alpha" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc rep_origin 2651..3106 /label="M13 origin" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 rep_origin 2668..2974 /label="F1 ori" /ApEinfo_revcolor=#0080c0 /ApEinfo_fwdcolor=#0080c0 CDS 4213..4872 /label="AmpR" /ApEinfo_revcolor=#ff4242 /ApEinfo_fwdcolor=#ff4242 rep_origin 4970..5652 /label="ColE1 origin" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 ORIGIN 1 CAGCTGCGCG CTCGCTCGCT CACTGAGGCC GCCCGGGCAA AGCCCGGGCG TCGGGCGACC 61 TTTGGTCGCC CGGCCTCAGT GAGCGAGCGA GCGCGCAGAG AGGGAGTGGG GTTCGGTACC 121 GCCTATTTCC CATGATTCCT TCATATTTGC ATATACGATA CAAGGCTGTT AGAGAGATAA 181 TTGGAATTAA TTTGACTGTA AACACAAAGA TATTAGTACA AAATACGTGA CGTAGAAAGT 241 AATAATTTCT TGGGTAGTTT GCAGTTTTAA AATTATGTTT TAAAATGGAC TATCATATGC 301 TTACCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA 361 ACACCGTACA CTAACACGCA TATTTGGTTT TAGTACTCTG GAAACAGAAT CTACTAAAAC 421 AAGGCAAAAT GCCGTGTTTA TCTCGTCAAC TTGTTGGCGA GATTTTTTTG CGGCCGCCCG 481 CGGTGGAGCT CCAGCTTTTG TTCCCTTTAG TGAGGGTTAA TCCTAGGGCC TATTTCCCAT 541 GATTCCTTCA TATTTGCATA TACGATACAA GGCTGTTAGA GAGATAATTG GAATTAATTT 601 GACTGTAAAC ACAAAGATAT TAGTACAAAA TACGTGACGT AGAAAGTAAT AATTTCTTGG 661 GTAGTTTGCA GTTTTAAAAT TATGTTTTAA AATGGACTAT CATATGCTTA CCGTAACTTG 721 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAACA CCGCATTGCA 781 TCCATGTCTG ACTGTTTTAG TACTCTGGAA ACAGAATCTA CTAAAACAAG GCAAAATGCC 841 GTGTTTATCT CGTCAACTTG TTGGCGAGAT TTTTTTGCGG CCGCCCGCGG TGGAGCTCCA 901 GCTTTTGTTC CCTTTAGTGA GGGTTAATTC TAGaggatcc ggtactcgag gaactgaaaa 961 accagaaagt taactggtaa gtttagtctt tttgtctttt atttcaggtc ccggatccgg 1021 tggtggtgca aatcaaagaa ctgctcctca gtggatgttg cctttacttc taggcctgta 1081 cggaagtgtt acttctgctc taaaagctgc ggaattgtac ccgcggcccg ggatccaCCG 1141 GTCGCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC 1201 GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT 1261 GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC 1321 TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC 1381 CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC 1441 ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC 1501 GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC 1561 CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG 1621 CAGAAGAACG GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG 1681 CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC 1741 GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT 1801 CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG 1861 TACAAGTAAA GCGGCCGCGC GGATCCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT 1921 GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC 1981 TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT 2041 GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ACAGCAGGCA TGCTGGGGAT 2101 GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT TTGGACGCGT AGGAACCCCT 2161 AGTGATGGAG TTGGCCACTC CCTCTCTGCG CGCTCGCTCG CTCACTGAGG CCGGGCGACC 2221 AAAGGTCGCC CGACGCCCGG GCTTTGCCCG GGCGGCCTCA GTGAGCGAGC GAGCGCGCAG 2281 CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA 2341 TGGCGAATGG CGATTCCGTT GCAATGGCTG GCGGTAATAT TGTTCTGGAT ATTACCAGCA 2401 AGGCCGATAG TTTGAGTTCT TCTACTCAGG CAAGTGATGT TATTACTAAT CAAAGAAGTA 2461 TTGCGACAAC GGTTAATTTG CGTGATGGAC AGACTCTTTT ACTCGGTGGC CTCACTGATT 2521 ATAAAAACAC TTCTCAGGAT TCTGGCGTAC CGTTCCTGTC TAAAATCCCT TTAATCGGCC 2581 TCCTGTTTAG CTCCCGCTCT GATTCTAACG AGGAAAGCAC GTTATACGTG CTCGTCAAAG 2641 CAACCATAGT ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 2701 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 2761 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 2821 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 2881 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 2941 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 3001 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 3061 CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGTTTA CAATTTAAAT ATTTGCTTAT 3121 ACAATCTTCC TGTTTTTGGG GCTTTTCTGA TTATCAACCG GGGTACATAT GATTGACATG 3181 CTAGTTTTAC GATTACCGTT CATCGATTCT CTTGTTTGCT CCAGACTCTC AGGCAATGAC 3241 CTGATAGCCT TTGTAGAGAC CTCTCAAAAA TAGCTACCCT CTCCGGCATG AATTTATCAG 3301 CTAGAACGGT TGAATATCAT ATTGATGGTG ATTTGACTGT CTCCGGCCTT TCTCACCCGT 3361 TTGAATCTTT ACCTACACAT TACTCAGGCA TTGCATTTAA AATATATGAG GGTTCTAAAA 3421 ATTTTTATCC TTGCGTTGAA ATAAAGGCTT CTCCCGCAAA AGTATTACAG GGTCATAATG 3481 TTTTTGGTAC AACCGATTTA GCTTTATGCT CTGAGGCTTT ATTGCTTAAT TTTGCTAATT 3541 CTTTGCCTTG CCTGTATGAT TTATTGGATG TTGGAATTCC TGATGCGGTA TTTTCTCCTT 3601 ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT 3661 GCCGCATAGT TAAGCCAGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT 3721 TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT 3781 CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGACGAA AGGGCCTCGT GATACGCCTA 3841 TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG 3901 GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG 3961 CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT 4021 ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT 4081 GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG 4141 GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA 4201 CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT 4261 GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG 4321 TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT 4381 GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA 4441 CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT 4501 TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA 4561 GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG 4621 CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC 4681 CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT 4741 ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG 4801 GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG 4861 ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA 4921 CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA 4981 ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA 5041 TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG 5101 CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT 5161 GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC 5221 CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG 5281 GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG 5341 GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA 5401 ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC 5461 GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG 5521 AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC 5581 TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC 5641 AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT 5701 CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC 5761 GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC 5821 CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATG //