LOCUS ptr_egfp_hu6_ai9 6440 bp ds-DNA circular 11-DEC-2020 DEFINITION . KEYWORDS "marker:AmpR, Kan/neoR, ITR, ITR" COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 3555..3555 /locus_tag="Additional T introduced in gblock" /label="Additional T introduced in gblock" /ApEinfo_label="Additional T introduced in gblock" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter complement(1679..1707) /locus_tag="Amp prom" /label="Amp prom" /ApEinfo_label="Amp prom" /ApEinfo_fwdcolor="#4682b4" /ApEinfo_revcolor="#4682b4" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3492..3554 /locus_tag="TracrRNA" /label="TracrRNA" /ApEinfo_label="TracrRNA" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS complement(780..1439) /locus_tag="AmpR" /label="AmpR" /ApEinfo_label="AmpR" /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3488..3491 /locus_tag="synthetic tetraloop" /label="synthetic tetraloop" /ApEinfo_label="synthetic tetraloop" /ApEinfo_fwdcolor="#000000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(3178..3197) /locus_tag="T3" /label="T3" /ApEinfo_label="T3" /ApEinfo_fwdcolor="#008b8b" /ApEinfo_revcolor="#008b8b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 3780..4493 /locus_tag="EGFP" /label="EGFP" /ApEinfo_label="EGFP" /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin 2084..2524 /locus_tag="F1 ori" /label="F1 ori" /ApEinfo_label="F1 ori" /ApEinfo_fwdcolor="#4682b4" /ApEinfo_revcolor="#4682b4" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 3204..3444 /locus_tag="U6 promoter" /label="U6 promoter" /ApEinfo_label="U6 promoter" /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" polyA_signal 4513..4704 /locus_tag="SV40 late polyA" /label="SV40 late polyA" /ApEinfo_label="SV40 late polyA" /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3145..3145 /locus_tag="Additional T introduced in gblock(1)" /label="Additional T introduced in gblock(1)" /ApEinfo_label="Additional T introduced in gblock" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3043..3063 /locus_tag="Ai9-Sa-gRNA1_With G" /label="Ai9-Sa-gRNA1_With G" /ApEinfo_label="Ai9-Sa-gRNA1_With G" /ApEinfo_fwdcolor="#3366ff" /ApEinfo_revcolor="#3366ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3474..3487 /locus_tag="crRNA Direct Repeat Portion +1 to +14" /label="crRNA Direct Repeat Portion +1 to +14" /ApEinfo_label="crRNA Direct Repeat Portion +1 to +14" /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 6055..6211 /locus_tag="ITR" /label="ITR" /ApEinfo_label="ITR" /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3064..3077 /locus_tag="crRNA Direct Repeat Portion +1 to +14(1)" /label="crRNA Direct Repeat Portion +1 to +14(1)" /ApEinfo_label="crRNA Direct Repeat Portion +1 to +14" /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin complement(2074..2529) /locus_tag="M13 origin" /label="M13 origin" /ApEinfo_label="M13 origin" /ApEinfo_fwdcolor="#4682b4" /ApEinfo_revcolor="#4682b4" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 5005..5796 /locus_tag="Kan/neoR" /label="Kan/neoR" /ApEinfo_label="Kan/neoR" /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3082..3144 /locus_tag="TracrRNA(1)" /label="TracrRNA(1)" /ApEinfo_label="TracrRNA" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3453..3473 /locus_tag="Ai9-Sa-gRNA2_With G" /label="Ai9-Sa-gRNA2_With G" /ApEinfo_label="Ai9-Sa-gRNA2_With G" /ApEinfo_fwdcolor="#3366ff" /ApEinfo_revcolor="#3366ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3078..3081 /locus_tag="synthetic tetraloop(1)" /label="synthetic tetraloop(1)" /ApEinfo_label="synthetic tetraloop" /ApEinfo_fwdcolor="#000000" /ApEinfo_revcolor="#000000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3474..3554 /locus_tag="sgRNA scaffold" /label="sgRNA scaffold" /ApEinfo_label="sgRNA scaffold" /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin complement(join(6440..6440,1..628)) /locus_tag="ColE1 origin" /label="ColE1 origin" /ApEinfo_label="ColE1 origin" /ApEinfo_fwdcolor="#4682b4" /ApEinfo_revcolor="#4682b4" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2613..2769 /locus_tag="ITR(1)" /label="ITR(1)" /ApEinfo_label="ITR" /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 2794..3034 /locus_tag="U6 promoter(1)" /label="U6 promoter(1)" /ApEinfo_label="U6 promoter" /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 3064..3144 /locus_tag="sgRNA scaffold(1)" /label="sgRNA scaffold(1)" /ApEinfo_label="sgRNA scaffold" /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 61 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 121 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 181 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg 241 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 301 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 361 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 421 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct 481 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 541 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 601 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 661 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 721 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 781 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 841 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 901 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 961 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 1021 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 1081 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 1141 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 1201 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 1261 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 1321 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 1381 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 1441 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 1501 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 1561 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 1621 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 1681 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 1741 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta 1801 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt 1861 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 1921 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt 1981 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg 2041 cacagatgcg taaggagaaa ataccgcatc aggaaattgt aaacgttaat attttgttaa 2101 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 2161 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 2221 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 2281 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 2341 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 2401 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 2461 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 2521 agggcgcgtc gcgccattcg ccattcaggc tacgcaactg ttgggaaggg cgatcggtgc 2581 gggcctcttc gctattacgc cagctggctg cagggggggg gggggggggg ttggccactc 2641 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg 2701 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca 2761 tcactagggg ttcctagatc tgaattcggt acCGAGGGCC TATTTCCCAT GATTCCTTCA 2821 TATTTGCATA TACGATACAA GGCTGTTAGA GAGATAATTA GAATTAATTT GACTGTAAAC 2881 ACAAAGATAT TAGTACAAAA TACGTGACGT AGAAAGTAAT AATTTCTTGG GTAGTTTGCA 2941 GTTTTAAAAT TATGTTTTAA AATGGACTAT CATATGCTTA CCGTAACTTG AAAGTATTTC 3001 GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAACA CCGCTCTAGA GTCGCAGATC 3061 CTCgttttag tactctggaa acagaatcta ctaaaacaag gcaaaatgcc gtgtttatct 3121 cgtcaacttg ttggcgagat tttttGCGGC CGCCCGCGGT GGAGCTCCAG CTTTTGTTCC 3181 CTTTAGTGAG GGTTAATCCT AGGGAGGGCC TATTTCCCAT GATTCCTTCA TATTTGCATA 3241 TACGATACAA GGCTGTTAGA GAGATAATTA GAATTAATTT GACTGTAAAC ACAAAGATAT 3301 TAGTACAAAA TACGTGACGT AGAAAGTAAT AATTTCTTGG GTAGTTTGCA GTTTTAAAAT 3361 TATGTTTTAA AATGGACTAT CATATGCTTA CCGTAACTTG AAAGTATTTC GATTTCTTGG 3421 CTTTATATAT CTTGTGGAAA GGACGAAACA CCGACGAAGT TATATTAAGG GTTgttttag 3481 tactctggaa acagaatcta ctaaaacaag gcaaaatgcc gtgtttatct cgtcaacttg 3541 ttggcgagat tttttTCTAG aggatccggt actcgaggaa ctgaaaaacc agaaagttaa 3601 ctggtaagtt tagtcttttt gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat 3661 caaagaactg ctcctcagtg gatgttgcct ttacttctag gcctgtacgg aagtgttact 3721 tctgctctaa aagctgcgga attgtacccg cggcccggga tccaccggtc gccaccatgg 3781 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg 3841 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca 3901 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg 3961 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc 4021 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca 4081 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga 4141 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc 4201 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca 4261 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc 4321 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc 4381 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc 4441 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg 4501 gccgcgggga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa 4561 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca 4621 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc 4681 agggggaggt gtgggaggtt ttttagtcga cctcgagcag tgtggttttg caagaggaag 4741 caaaaagcct ctccacccag gcctggaatg tttccaccca agtcgaaggc agtgtggttt 4801 tgcaagagga agcaaaaagc ctctccaccc aggcctggaa tgtttccacc caatgtcgag 4861 caaccccgcc cagcgtcttg tcattggcga attcgaacac gcagatgcag tcggggcggc 4921 gcggtcccag gtccacttcg catattaagg tgacgcgtgt ggcctcgaac accgagcgac 4981 cctgcagcca atatgggatc ggccattgaa caagatggat tgcacgcagg ttctccggcc 5041 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 5101 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 5161 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg 5221 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 5281 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 5341 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 5401 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 5461 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 5521 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 5581 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 5641 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 5701 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 5761 atcgccttct atcgccttct tgacgagttc ttctgagggg atccgtcgac tagagctcgc 5821 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg 5881 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt 5941 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc 6001 aagggggagg attgggaaga caatagcagg catgctgggg agagatctag gaacccctag 6061 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gcccgggcaa 6121 agcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag 6181 agggagtggc caaccccccc cccccccccc ctgcagccca gctgcattaa tgaatcggcc 6241 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 6301 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 6361 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 6421 aggccaggaa ccgtaaaaag //