LOCUS Exported 17625 bp DNA circular SYN 07-OCT-2022 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 17625) AUTHORS . TITLE Direct Submission JOURNAL Exported Oct 7, 2022 from SnapGene 6.0.5 https://www.snapgene.com FEATURES Location/Qualifiers source 1..17625 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 4630..4770 /label=splice acceptor /note="Oriinal location: 1126..1266" /note="color: #a6acb3" polyA_signal 4823..5342 /label=SV40 late poly A /note="Oriinal location: 1319..1828" /note="color: #a6acb3" misc_feature 5361..5408 /label=FRT /note="Oriinal location: 1847..1894" /note="color: #a6acb3" promoter 5423..7089 /label=CAG Promoter /note="Oriinal location: 87..1753" /note="/vntifkey=21" /note="color: #ffffff" CDS 7108..7827 /codon_start=1 /label=Venus /note="Oriinal location: 88..807" /note="color: #00ff00" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMK*HDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" misc_feature complement(7335..7354) /label=sgRNA target sequence for A base editor /note="color: #800000; direction: LEFT" CDS 7348 /codon_start=1 /label=G>A = stop /note="color: #ff0000" /translation="" misc_feature 7870..8141 /label=bpA /note="Oriinal location: 2900..3171 pA from bovine growth hormone" /note="/vntifkey=25" /note="color: #a6acb3" misc_feature 8176..8382 /label=SV40 pA /note="Oriinal location: 2044..2250" /note="color: #a6acb3" ORIGIN 1 tctagaacca ttacatttat gggatctaaa taaatttgta caaaattaaa cagactcccg 61 aaggaaaagg cattggttgc agtaataatc ctagggagga aaaaccctac gattttatgt 121 gctgcacatg cctgggtgat ggttcagttt caaatattcc ttgccagaca aactacttaa 181 aaaggcttaa tcaaagatca ccctggttct atttggttag gggtagggtg ggcttagaat 241 ggactcttgc tgccttctta gtatcttgaa ctaaacttac tcaatttctg aatattcctt 301 ctgtgtctct gatggattac tgccaaaaca catctttgtt cggtggaaag tctataatcc 361 aacacataca cgattaatcc aatttatagg tcagatatca gtaagtatat ctaatctgga 421 gaggtctaca gattaattta gaattaacaa caaaccaagc attaatccaa aagcagctct 481 ttttagttta ggcatccaaa tatatagtaa gatgtttcaa agaacatcaa tctattcaca 541 aaggtaaata cggcctaata cggagagaca aatacaaatc tgaaatgact caagcaatta 601 aaagatggag gaattcatgt cacttgcact gggaaaacaa aacaaagatc ctcctcaccc 661 tattacccac attactattt ttaggttttt taggggttaa aaaagatcaa aacactaatg 721 aactttaagt cctgtgaagg gtaaaacctc agatagtaac aaaaagcttc caacccctcc 781 tcaaacaaaa aaccccaagt ctttaacttt gatccagttt tcagatgctg atatccataa 841 atggatacag ttatgaattg ctaattctgg tctcttcact agcaaaaagc aaagcagctc 901 agcagtacaa tttcccagga aagcaagcaa ggtttctttc cagcctgagc agccatcact 961 aagtgcagtt ccctgcagcc aacagcatta atggacgctg cactgctgtc cttccctgga 1021 gacagcagcc agcactactc aagcttctca cgtagcaacc agagctccag agccagcagc 1081 tgctgccgcc ttgtatactc actcctgtga tccaacacag gagcaacctt ttctttaccc 1141 cacccccact tcttaacaca cttttttttg gggggggggg ggaacaagtg ctccatgctg 1201 gaaggattgg aactatgctt ttagaaagga acaatcctaa ggtcactttt aaattgaggt 1261 ctttgatttg aaaatcaaca aataccaaat tccaaatatt cgttttaatt aaaccagcaa 1321 tgtggatata agcattaagt tttagtttta aaaaggtcaa ttttccaaac attcagcaat 1381 catatttaaa tttacagcta ggaacaagag ccttgggtca tgtcctacca aagaacataa 1441 ctcaatattc tacacatgac aatctgaata accttaaagc ctctaatccc ataacaggcc 1501 acaaattttg gacagagaac taatgatcct cctgagaaaa ctggaagaaa tccagggaaa 1561 agaaattcct gtgtcctcca aactcagaaa tctctaatta tgtcagtatt ctctgcttta 1621 gtcctaggtc agattgcaca catctaaaat aacctcttaa agtttcctcc tagcgaccta 1681 aaccattatt aatatcaaat taaccatcaa aacactttcc tctcaatatg ctgcacacaa 1741 acctcctcct ggaacctcct ccatctggat cctccccaat caaaagtata ggtatttaac 1801 atataagcaa ggaagtaatg taaacatgac cttggtcaca aatatgtcat ctaaaaacaa 1861 tttagtcaag gtatggagga aattcgagaa cctgaatctt tttaagtatt ttgagcacag 1921 gaacaattgg caaaaggaat ccaggtatag acaaaaccca gagcccagag ctctgggcga 1981 aaaatgagtt gctggtgaag acgttacaca agtaacatga gaaagcagaa aatgcaggtc 2041 atccacgcac ccctgaccca ggccagcagg gcgggctgca gcatcagtac acaggagaaa 2101 gatccttatt cctaagaatg agaaaggcaa aggcgcccga tagaataaat tagcatagaa 2161 ggggctttcc caggagttaa aactttcctt ctgagcgatt acctactaaa accagggctt 2221 ttgcccacta ccatttacct aggatcttgg cttgcacgga ttcatagggg catatccctc 2281 cccctcttct ttagagtcgt tcttaaaaga tcgctctcca cgccctaggc agggaaaacg 2341 acaaaatctg gctcaattcc aggctagaac cctacaaatt caacagggat atcgcaagga 2401 tactggggca tacgccacag ggagtccaag aatgtgaggt gggggtggcg aaggtaatgt 2461 ctttggtgtg ggaaaagcag cagccatctg agataggaac tggaaaacca gaggagaggc 2521 gttcaggaag attatggagg ggaggactgg gcccccacga gcgaccagag ttgtcacaag 2581 gccgcaagaa caggggaggt ggggggctca gggacagaaa aaaaagtatg tgtattttga 2641 gagcagggtt gggaggcctc tcctgaaaag ggtataaacg tggagtaggc aatacccagg 2701 caaaaagggg agaccagagt agggggaggg gaagagtcct gacccaggga agacattaaa 2761 aaggtagtgg ggtcgactag atgaaggaga gcctttctct ctgggcaaga gcggtgcaat 2821 ggtgtgtaaa ggtagctgag aagacgaaaa gggcaagcat cttcctgcta ccaggctggg 2881 gaggcccagg cccacgaccc cgaggagagg gaacgcaggg agactgaggt gacccttctt 2941 tcccccgggg cccggtcgtg tggttcggtg tctcttttct gttggaccct taccttgacc 3001 caggcgctgc cggggcctgg gcccgggctg cggcgcacgg cactcccggg aggcagcgag 3061 actcgagtta ggcccaacgc ggcgccacgg cgtttcctgg ccgggaatgg cccgtacccg 3121 tgaggtgggg gtggggggca gaaaaggcgg agcgagcccg aggcggggag ggggagggcc 3181 aggggcggag ggggccggca ctactgtgtt ggcggactgg cgggactagg gctgcgtgag 3241 tctctgagcg cagcgggcgg cggccgcccc tcccccggcg gcggcagcgg cggcagcggc 3301 ggcagctcac tcagcccgct gcccgagcgg aaacgccact gaccgcacgg ggattcccag 3361 tgccggcgcc aggggcacgc gggacacgcc ccctcccgcc gcgccattgg cctctccgcc 3421 caccgcccca cacttattgg ccggtgcgcc gccaatcagc ggaggctgcc ggggccgcct 3481 aaagaagagg ctgtgctttg gggctccggc tcctcagaga gcctcggcta ggtaggggat 3541 cgggactctg gcgggagggc ggcttggtgc gtttgcgggg atgggcggcc gcggcaggcc 3601 ctccgagcgt ggtggagccg ttctgtgaga cagccgggta cgagtcgtga cgctggaagg 3661 ggcaagcggg tggtgggcag gaatgcggtc cgccctgcag caaccggagg gggagggaga 3721 agggagcgga aaagtctcca ccggacgcgg ccatggctcg gggggggggg ggcagcggag 3781 gagcgcttcc ggccgacgtc tcgtcgctga ttggcttctt ttcctcccgc cgtgtgtgaa 3841 aacacaaatg gcgtgttttg gttggcgtaa ggcgcctgtc agttaacggc agccggagtg 3901 cgcagccgcc ggcagcctcg ctctgcccac tgggtggggc gggaggtagg tggggtgagg 3961 cgagctggac gtgcgggcgc ggtcggcctc tggcggggcg ggggagggga gggagggtca 4021 gcgaaagtag ctcgcgcgcg agcggccgcc caccctcccc ttcctctggg ggagtcgttt 4081 tacccgccgc cggccgggcc tcgtcgtctg attggctctc ggggcccaga aaactggccc 4141 ttgccattgg ctcgtgttcg tgcaagttga gtccatccgc cggccagcgg gggcggcgag 4201 gaggcgctcc caggttccgg ccctcccctc ggccccgcgc cgcagagtct ggccgcgcgc 4261 ccctgcgcaa cgtggcagga agcgcgcgct gggggcgggg acgggcagta gggctgagcg 4321 gctgcggggc gggtgcaagc acgtttccga cttgagttgc ctcaagaggg gcgtgctgag 4381 ccagacctcc atcgcgcact ccggggagtg gagggaagga gcgagggctc agttgggctg 4441 ttttggaggc aggaagcact tgctctccca aagtcgctct gagttgttat cagtaaggga 4501 gctgcagtgg agtaggcggg gagaaggccg cacccttctc cggagggggg aggggagtgt 4561 tgcaatacct ttctgggagt tctctgctgc ctcctggctt ctgaggaccg ccctggcgcc 4621 cgggctgcag atctgtaggg cgcagtagtc cagggtttcc ttgatgatgt catacttatc 4681 ctgtcccttt tttttccaca gctcgcggtt gaggacaaac tcttcgcggt ctttccagtg 4741 gggatcgacg gtatcgataa gctggccgct ctagtggccg tacgggccca cctgccgggc 4801 cacttaatta aatttaaatc acaattccag ctgagcgccg gtcgctacca ttaccagttg 4861 gtctggtgtc aaaaataata ataaccgggc aggggggatc tgcatggatc tttgtgaagg 4921 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta 4981 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg 5041 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gccagatcca 5101 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa 5161 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat 5221 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg 5281 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctgcgg 5341 ccgtgctagc gcttaagctt gaagttccta ttccgaagtt cctattctct agaaagtata 5401 ggaacttcgg cgcgccggat tcgacattga ttattgacta gttattaata gtaatcaatt 5461 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 5521 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 5581 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 5641 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 5701 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 5761 acttggcagt acatctacgt attagtcatc gctattacca tgggtcgagg tgagccccac 5821 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat 5881 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg 5941 cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga 6001 gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa 6061 agcgaagcgc gcggcgggcg ggagtcgctg cgttgccttc gccccgtgcc ccgctccgcg 6121 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc 6181 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg ctcgtttctt 6241 ttctgtggct gcgtgaaagc cttaaagggc tccgggaggg ccctttgtgc gggggggagc 6301 ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg cccgcgctgc 6361 ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc gtgtgcgcga 6421 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg gctgcgaggg gaacaaaggc 6481 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcggc ggtcgggctg 6541 taaccccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg 6601 gctccgtgcg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg 6661 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg 6721 ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa 6781 tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct ggcggagccg aaatctggga 6841 ggcgccgccg caccccctct agcgggcgcg ggcgaagcgg tgcggcgccg gcaggaagga 6901 aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc atctccagcc 6961 tcggggctgc cgcaggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc 7021 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt 7081 ttcctacaga tccttaatta agccaccatg gtgagcaagg gcgaggagct gttcaccggg 7141 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc 7201 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc 7261 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc 7321 ttcgcccgct accccgacca catgaagtag cacgacttct tcaagtccgc catgcccgaa 7381 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc 7441 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc 7501 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc 7561 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac 7621 atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac 7681 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac 7741 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact 7801 ctcggcatgg acgagctgta caagtaagcg atcgcacgcg taaatgattg cagatccact 7861 agttctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 7921 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 7981 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 8041 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg 8101 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgagcct ctagaactat 8161 agtgagtcgt attacgtaga tccagacatg ataagataca ttgatgagtt tggacaaacc 8221 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta 8281 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg 8341 tttcaggttc agggggaggt gtgggaggtt ttttaattcg cggccctaga agatgggcgg 8401 gagtcttctg ggcaggctta aaggctaacc tggtgtgtgg gcgttgtcct gcaggggaat 8461 tgaacaggtg taaaattgga gggacaagac ttcccacaga ttttcggttt tgtcgggaag 8521 ttttttaata ggggcaaata aggaaaatgg gaggataggt agtcatctgg ggttttatgc 8581 agcaaaacta caggttatta ttgcttgtga tccgcctcgg agtattttcc atcgaggtag 8641 attaaagaca tgctcacccg agttttatac tctcctgctt gagatcctta ctacagtatg 8701 aaattacagt gtcgcgagtt agactatgta agcagaattt taatcatttt taaagagccc 8761 agtacttcat atccatttct cccgctcctt ctgcagcctt atcaaaaggt attttagaac 8821 actcatttta gccccatttt catttattat actggcttat ccaaccccta gacagagcat 8881 tggcattttc cctttcctga tcttagaagt ctgatgactc atgaaaccag acagattagt 8941 tacatacacc acaaatcgag gctgtagctg gggcctcaac actgcagttc ttttataact 9001 ccttagtaca ctttttgttg atcctttgcc ttgatcctta attttcagtg tctatcacct 9061 ctcccgtcag gtggtgttcc acatttgggc ctattctcag tccagggagt tttacaacaa 9121 tagatgtatt gagaatccaa cctaaagctt aactttccac tcccatgaat gcctctctcc 9181 tttttctcca tttataaact gagctattaa ccattaatgg tttccaggtg gatgtctcct 9241 cccccaatat tacctgatgt atcttacata ttgccaggct gatattttaa gacattaaaa 9301 ggtatatttc attattgagc cacatggtat tgattactgc ttactaaaat tttgtcattg 9361 tacacatctg taaaaggtgg ttccttttgg aatgcaaagt tcaggtgttt gttgtctttc 9421 ctgacctaag gtcttgtgag cttgtatttt ttctatttaa gcagtgcttt ctcttggact 9481 ggcttgactc atggcattct acacgttatt gctggtctaa atgtgatttt gccaagcttc 9541 ttcaggacct ataattttgc ttgacttgta gccaaacaca agtaaaatga ttaagcaaca 9601 aatgtatttg tgaagcttgg tttttaggtt gttgtgttgt gtgtgcttgt gctctataat 9661 aatactatcc aggggctgga gaggtggctc ggagttcaag agcacagact gctcttccag 9721 aagtcctgag ttcaattccc agcaaccaca tggtggctca caaccatctg taatgggatc 9781 tgatgccctc ttctggtgtg tctgaagacc acaagtgtat tcacattaaa taaataaatc 9841 ctccttcttc ttcttttttt tttttttaaa gagaatactg tctccagtag aatttactga 9901 agtaatgaaa tactttgtgt ttgttccaat atggtagcca ataatcaaat tactctttaa 9961 gcactggaaa tgttaccaag gaactaattt ttatttgaag tgtaactgtg gacagaggag 10021 ccataactgc agacttgtgg gatacagaag accaatgcag actttaatgt cttttctctt 10081 acactaagca ataaagaaat aaaaattgaa cttctagtat cctatttgtt taaactgcta 10141 gctttactta acttttgtgc ttcatctata caaagctgaa agctaagtct gcagccatta 10201 ctaaacatga aagcaagtaa tgataatttt ggatttcaaa aatgtagggc cagagtttag 10261 ccagccagtg gtggtgcttg cctttatgcc tttaatccca gcactctgga ggcagagaca 10321 ggcagatctc tgagtttgag cccagcctgg tctacacatc aagttctatc taggatagcc 10381 aggaatacac acagaaaccc tgttggggag gggggctctg agatttcata aaattataat 10441 tgaagcattc cctaatgagc cactatggat gtggctaaat ccgtctacct ttctgatgag 10501 atttgggtat tattttttct gtctctgctg ttggttgggt cttttgacac tgtgggcttt 10561 ctttaaagcc tccttcctgc catgtggtct cttgtttgct actaacttcc catggcttaa 10621 atggcatggc tttttgcctt ctaagggcag ctgctgagat ttgcagcctg atttccaggg 10681 tggggttggg aaatctttca aacactaaaa ttgtccttta attttttttt taaaaaatgg 10741 gttatataat aaacctcata aaatagttat gaggagtgag gtggactaat attaaatgag 10801 tccctcccct ataaaagagc tattaaggct ttttgtctta tacttaactt tttttttaaa 10861 tgtggtatct ttagaaccaa gggtcttaga gttttagtat acagaaactg ttgcatcgct 10921 taatcagatt ttctagtttc aaatccagag aatccaaatt cttcacagcc aaagtcaaat 10981 taagaatttc tgacttttaa tgttaatttg cttactgtga atataaaaat gatagctttt 11041 cctgaggcag ggtctcacta tgtatctctg cctgatctgc aacaagatat gtagactaaa 11101 gttctgcctg cttttgtctc ctgaatacta aggttaaaat gtagtaatac ttttggaact 11161 tgcaggtcag attcttttat aggggacaca ctaagggagc ttgggtgata gttggtaaaa 11221 tgtgtttcaa gtgatgaaaa cttgaattat tatcaccgca acctactttt taaaaaaaaa 11281 agccaggcct gttagagcat gcttaaggga tccctaggac ttgctgagca cacaagagta 11341 gttacttggc aggctcctgg tgagagcata tttcaaaaaa caaggcagac aaccaagaaa 11401 ctacagttaa ggttacctgt ctttaaacca tctgcatata cacagggata ttaaaatatt 11461 ccaaataata tttcattcaa gttttccccc atcaaattgg gacatggatt tctccggtga 11521 ataggcagag ttggaaacta aacaaatgtt ggttttgtga tttgtgaaat tgttttcaag 11581 tgatagttaa agcccatgag atacagaaca aagctgctat ttcgaggtct cttggtttat 11641 actcagaagc acttctttgg gtttccctgc actatcctga tcatgtgcta ggcctacctt 11701 aggctgattg ttgttcaaat aaacttaagt ttcctgtcag gtgatgtcat atgatttcat 11761 atatcaaggc aaaacatgtt atatatgtta aacatttgta cttaatgtga aagttaggtc 11821 tttgtgggtt tgatttttaa ttttcaaaac ctgagctaaa taagtcattt ttacatgtct 11881 tacatttggt ggaattgtat aattgtggtt tgcaggcaag actctctgac ctagtaaccc 11941 tacctataga gcactttgct gggtcacaag tctaggagtc aagcatttca ccttgaagtt 12001 gagacgtttt gttagtgtat actagtttat atgttggagg acatgtttat ccagaagata 12061 ttcaggacta tttttgactg ggctaaggaa ttgattctga ttagcactgt tagtgagcat 12121 tgagtggcct ttaggcttga attggagtca cttgtatatc tcaaataatg ctggcctttt 12181 ttaaaaagcc cttgttcttt atcaccctgt tttctacata atttttgttc aaagaaatac 12241 ttgtttggat ctccttttga caacaatagc atgttttcaa gccatatttt ttttcctttt 12301 tttttttttt tttggttttt cgagacaggg tttctctgta tagccctggc tgtcctggaa 12361 ctcactttgt agaccaggct ggcctcgaac tcagaaatcc gcctgcctct gcctcctgag 12421 tgccgggatt aaaggcgtgc accaccacgc ctggctaagt tggatatttt gttatataac 12481 tataaccaat actaactcca ctgggtggat ttttaattca gtcagtagtc ttaagtggtc 12541 tttattggcc cttcattaaa atctactgtt cactctaaca gaggctgttg gtactagtgg 12601 cacttaagca acttcctacg gatatactag cagattaagg gtcagggata gaaactagtc 12661 tagcgttttg tatacctacc agctttatac taccttgttc tgatagaaat atttcaggac 12721 atctagagtg tactataagg ttgatggtaa gcttataagg aacttgaaag tggagtaact 12781 actccatttc tctgagggga gaattaaaat ttttgaccaa gtgttgttga gccactgaga 12841 atggtctcag aacataactt cttaaggaac cttcccagat tgccctcaac actgcaccac 12901 atttggtcct gcttgaacat tgccatggct cttaaagtct taattaagaa tattaattgt 12961 gtaattattg tttttcctcc tttagatcat tccttgagga caggacagtg cttgtttaag 13021 gctatatttc tgctgtctga gcagcaacag gtcttcgaga tcaacatgat gttcataatc 13081 ccaagatgtt gccatttatg ttctcagaag caagcagagg catgatggtc agtgacagta 13141 atgtcactgt gttaaatgtt gctatgcagt ttggattttt ctaatgtagt gtaggtagaa 13201 catatgtgtt ctgtatgaat taaactctta agttacacct tgtataatcc atgcaatgtg 13261 ttatgcaatt accattttaa gtattgtagc tttctttgta tgtgaggata aaggtgtttg 13321 tcataaaatg ttttgaacat ttccccaaag ttccaaatta taaaaccaca acgttagaac 13381 ttatttatga acaatggttg tagtttcatg cttttaaaat gcttaattat tcaattaaca 13441 ccgtttgtgt tataatatat ataaaactga catgtagaag tgtttgtcca gaacatttct 13501 taaatgtata ctgtctttag agagtttaat atagcatgtc ttttgcaaca tactaacttt 13561 tgtgttggtg cgagcaatat tgtgtagtca ttttgaaagg agtcatttca atgagtgtca 13621 gattgttttg aatgttattg aacattttaa atgcagactt gttcgtgttt tagaaagcaa 13681 aactgtcaga agctttgaac tagaaattaa aaagctgaag tatttcagaa gggaaataag 13741 ctacttgctg tattagttga aggaaagtgt aatagcttag aaaatttaaa accatatagt 13801 tgtcattgct gaatatctgg cagatgaaaa gaaatactca gtggttcttt tgagcaatat 13861 aacagcttgt tatattaaaa attttcccca cagatataaa ctctaatcta taactcataa 13921 atgttacaaa tggatgaagc ttacaaatgt ggcttgactt gtcactgtgc ttgttttagt 13981 tatgtgaaag tttggcaata aacctatgtc ctaaatagtc aaactgtgga atgacttttt 14041 aatctattgg tttgtctaga acagttatgt tgccatttgc cctaatggtg aaagaaaaag 14101 tggggagtgc cttggcactg ttcatttgtg gtgtgaacca aagagggggg catgcactta 14161 cacttcaaac atccttttga aagactgaca agtttgggtc ttcacagttg gaattgggca 14221 tcccttttgt cagggaggga gggagggagg gaggctggct tgttatgctg acaagtgtga 14281 ttaaattcaa actttgaggt aagttggagg aacttgtaca ttgttaggag tgtgacaatt 14341 tggactctta atgatttggt catacaaaat gaacctagac caacttctgg aagatgtata 14401 taataactcc atgttacatt gatttcacct gactaatact tatcccttat caattaaata 14461 cagaagatgc cagccatctg ggccttttaa cccagaaatt tagtttcaaa ctcctaggtt 14521 agtgttctca ctgagctaca tcctgatcta gtcctgaaaa taggaccacc atcaccccca 14581 aaaaaatctc aaataagatt tatgctagtg tttcaaaatt ttaggaatag gtaagattag 14641 aaagttttaa attttgagaa atggcttctc tagaaagatg tacatagtga acactgaatg 14701 gctcctaaag agcctagaaa actggtactg agcacacagg actgagaggt ctttcttgaa 14761 aagcatgtat tgctttacgt gggtcacaga aggcaggcag gaagaacttg ggctgaaact 14821 ggtgtcttaa gtggctaaca tcttcacaac tgatgagcaa gaactttatc ctgatgcaaa 14881 aaccatccaa acaaactaag tgaaaggtgg caatggatcc caggctgctc tagaggagga 14941 cttgacttct catcccatca cccacaccag atagctcata gactgccaat taacaccagc 15001 ttctagcctc cacaggcacc tgcactggta cacataattt cacacaaaca cagtaagaag 15061 ccttccacct ggcatggtat tgcttatctt tagttcccaa cacttgggag gcagaggcca 15121 gccagggcta tgtgacaaaa accttgtcta gaggagaaac ttcatagctt atttcctatt 15181 cacgtaacca ggttagcaaa atttaccagc cagagatgaa gctaacagtg tccactatat 15241 ttgtagtgtt ttaagtcaat tttttaaata tacttaatag aattaaagct atggtgaacc 15301 aagtacaaac ctggtgtatt aacttgagaa cttagcataa aaagtagttc atttgttcag 15361 taaatattaa atgcttactg gcaaagatta tgtcaggaac ttggtaaatg gtgatgaaac 15421 aatcatagtt gtacatcttg gttctgtgat caccttggtt tgaggtaaaa gtggttcctt 15481 tgatcaagga tggaatttta agtttatatt caatcaataa tgtattattt tgtgattgca 15541 aaattgccta tctagggtat aaaaccttta aaaatttcat aataccagtt cattctccag 15601 ttactaattc caaaaagcca ctgactatgg tgccaatgtg gattctgttc tcaaaggaag 15661 gattgtctgt gccctttatt ctaatagaaa catcacactg aaaatctaag ctgaaagaag 15721 ccagactttc ctaaataaat aactttccat aaagctcaaa caaggattac ttttaggagg 15781 cactgttaag gaactgataa gtaatgaggt tacttatata atgatagtcc cacaagacta 15841 tctgaggaaa aatcagtaca actcgaaaac agaacaacca gctaggcagg aataacaggg 15901 ctcccaagtc aggaggtcta tccaacaccc ttttctgttg agggccccag acctacatat 15961 tgtatacaaa cagggaggtg ggtgatttta actctcctga ggtaccttgg taaatctttg 16021 tcctgagtaa gcagtacagt gtacagttta cattttcatt taaagataca ttagctccct 16081 ctacccccta agactgacag gcactttggg ggtggggagg gctttggaaa ataacgcttc 16141 catacactaa aagagaaatt tctttaatta ggcttgttgg ttccatacat ctactggtgt 16201 ttctactact tagtaatatt ataatagtca cacaagcatc tttgctctgt ttaggttgta 16261 tatttatttt aaggcagatg ataaaactgt agatcttaag ggatgcttct gcttctgaga 16321 tgatacaaag aatttagacc ataaaacagt aggttgcaca agcaatagaa tatggcctaa 16381 agtgttctga cacttagaag ccaagcagtg taggcttctt aagaaatacc attacaatca 16441 ccttgctaga aatcaagcat tctggagtgg tcaagcagtg taacctgtac tgtaagttac 16501 ttttctgcta tttttctccc aaagcaagtt ctttatgctg atatttccag tgttaggaac 16561 tacaaatatt aataagttgt cttcactctt ttctttacca aggagggtct cttccttcat 16621 cttgatctga aggatgaaca aaggcttgag cagtgcgctt tagaagataa actgcagcat 16681 gaaggccccc gatgttcacc cagactacat ggacctttcg ccacacatgt cccattccag 16741 ataaggcctg gcacacacaa aaaacataag tcattaggct accagtctga ttctaaaaca 16801 acctaaaatc ttcccactta aatgctatgg gtggtgggtt ggaaagttga ctcagaaaat 16861 cacttgctgt ttttagagag gatctgggtt cagtttctga tacattgtgg cttacaacta 16921 taactccagt tctagggggt ccatccaaca tcctcttctg ttgagggcac caaataaatg 16981 tattgtgtac aaacagggag gtgagtgatt taactctcgt gtatagtacc ttggtaaaac 17041 atttcttgtc ctgagtaagc agtacagctc tgcctgtccc tggtctacag acacggctca 17101 tttcccgaag gcaagctgga tagagattcc aatttctctt cttggatccc atcctataaa 17161 agaaggtcaa gtttaatcta ttgcaaaagg taaataggta gtttcttaca tgagacaaga 17221 acaaatctta ggtgtgaagc agtcatcttt tacaggccag agcctctatt ctatgccaat 17281 gaaggaaact gttagtccag tgttatagag ttagtccagt gtatagtttt ctatcagaac 17341 actttttttt taaacaactg caacttagct tattgaagac aaaccacgag tagaaatctg 17401 tccaagaagc aagtgcttct cagcctacaa tgtggaatag gaccatgtaa tggtacagtg 17461 agtgaaatga attatggcat gtttttctga ctgagaagac agtacaataa aaggtaaact 17521 catggtattt atttaaaaag aatccaattt ctaccttttt ccaaatggca tatctgttac 17581 aataatatcc acagaagcag ttctcagtgg gaggttgcag atatc //