LOCUS Exported 16432 bp DNA linear SYN 12-OCT-2022 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 16432) AUTHORS . TITLE Direct Submission JOURNAL Exported Oct 12, 2022 from SnapGene 6.0.5 https://www.snapgene.com FEATURES Location/Qualifiers source 1..16432 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature join(1..8359,14129..15432) /label=Gt(ROSA)26Sor - MGI:104735 /note="Gt(ROSA)26Sor - MGI:104735" /note="This feature has 2 segments: 1: 1 .. 8359 / #ff6666 2: 14129 .. 15432 / #ff6666" misc_feature 8360..8384 /label=Bxb1 MUT attP /note="color: #ff0000" misc_feature 8383..8384 /label=GA /note="color: #a6acb3" misc_feature complement(8385..8402) /label=Bxb1 attB(BB') SITE (GA) /note="color: #ff6600; direction: LEFT" enhancer 8453..8832 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" /note="color: #ffffff" promoter 8835..9110 /label=chicken beta-actin promoter /note="color: #ffffff; direction: RIGHT" intron 9111..10119 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" /note="color: #------" regulatory 10186..10195 /label=Kozak sequence /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /note="color: #a6acb3" /regulatory_class="other" CDS 10195..10236 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /note="color: #cc99b2" /translation="GKPIPNPLLGLDST" CDS 10243..10284 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /note="color: #cc99b2" /translation="GKPIPNPLLGLDST" CDS 10291..10332 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /note="color: #cc99b2" /translation="GKPIPNPLLGLDST" misc_feature 10334..10614 /label=Venus 5' /note="Having a hard time lining this up exactly. need to check original file for certainty. this will do for now." /note="color: #00ff00" misc_feature 10624..10643 /label=Guide 12-2 /note="color: #800000; direction: RIGHT" misc_feature 10625..10644 /label=Guide 12-1 /note="color: #800000; direction: RIGHT" misc_feature 10662..11098 /label=Venus 3' /note="Picks up codon 98-end. Is in +3 reading frame for Snapgene. +2 reading frame for indelphi. " /note="color: #00ff00" CDS 11099..11155 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /note="color: #cc99b2 Cleavage site after base 11152" /translation="ATNFSLLKQAGDVEENPGP" CDS 11159..11863 /codon_start=1 /product="improved version of the oligomeric far-red fluorescent protein TurboFP635/Katushka (Luker et al., 2015)" /label=Katushka2S /note="mammalian codon-optimized" /note="color: #a0003c" /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV ARYCDLPSKLGHS" CDS 11864..11920 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /note="color: #cc99b2 Cleavage site after base 11917" /translation="ATNFSLLKQAGDVEENPGP" CDS 11921..13570 /codon_start=1 /label=akaLuciferase cds /note="color: #ffcc00" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGAIAFTDA HIQVDVTYAEYFEMSVRLAEAMRRYGLNTNHRIVVCSENSSQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLRKVLNVQKKLPIIRKIIIMDSKTDYQGFQS MYTFVTSHLPPSFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGYQNIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSCLAKSTLIDKYDLSSLREIASGGAPLSKEVGEAVAKRFRLPGIRQGY GLTETTNAVMITPEGDRKPGSVGKVVPFFEAKVVDLVTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELEGIL LQHPYIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPRGSTGKLDARKIREILTKAKKDGKIAV" CDS 13571..13594 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /note="color: #cc99b2" /translation="DYKDDDDK" CDS 13601..13624 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /note="color: #cc99b2" /translation="DYKDDDDK" CDS 13631..13654 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /note="color: #cc99b2" /translation="DYKDDDDK" polyA_signal 13719..13943 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" /note="color: #a6acb3" misc_feature complement(14080..14097) /label=BxbI attB(BB') SITE (GT) /label=BxbI AttB /note="/ApEinfo_fwdcolor=#ffee30 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /ApEinfo_revcolor=green" /note="color: #00ff00; direction: LEFT" misc_feature 14098..14122 /label=Bxb1 AttP Site /note="Bxb1 AttP Site" /note="color: #ff0000" misc_feature 14098..14099 /label=GT /note="color: #a6acb3" misc_feature 14123..14128 /label=INDEL /note="GET 314 INS" /note="color: #ffff00" ORIGIN 1 tttgtacaca atacatttat ttggtgccct caacagaaga ggatgttgga tggaccccct 61 agaactggag ttatagttgt aagccacaat gtatcagaaa ctgaacccag atcctctcta 121 aaaacagcaa gtgattttct gagtcaactt tccaacccac cacccatagc atttaagtgg 181 gaagatttta ggttgtttta gaatcagact ggtagcctaa tgacttatgt tttttgtgtg 241 tgccaggcct tatctggaat gggacatgtg tggcgaaagg tccatgtagt ctgggtgaac 301 atcgggggcc ttcatgctgc agtttatctt ctaaagcgca ctgctcaagc ctttgttcat 361 ccttcagatc aagatgaagg aagagaccct ccttggtaaa gaaaagagtg aagacaactt 421 attaatattt gtagttccta acactggaaa tatcagcata aagaacttgc tttgggagaa 481 aaatagcaga aaagtaactt acagtacagg ttacactgct tgaccactcc agaatgcttg 541 atttctagca aggtgattgt aatggtattt cttaagaagc ctacactgct tggcttctaa 601 gtgtcagaac actttaggcc atattctatt gcttgtgcaa cctactgttt tatggtctaa 661 attctttgta tcatctcaga agcagaagca tcccttaaga tctacagttt tatcatctgc 721 cttaaaataa atatacaacc taaacagagc aaagatgctt gtgtgactat tataatatta 781 ctaagtagta gaaacaccag tagatgtatg gaaccaacaa gcctaattaa agaaatttct 841 cttttagtgt atggaagcgt tattttccaa agccctcccc acccccaaag tgcctgtcag 901 tcttaggggg tagagggagc taatgtatct ttaaatgaaa atgtaaactg tacactgtac 961 tgcttactca ggacaaagat ttaccaaggt acctcaggag agttaaaatc acccacctcc 1021 ctgtttgtat acaatatgta ggtctggggc cctcaacaga aaagggtgtt ggatagacct 1081 cctgacttgg gagccctgtt attcctgcct agctggttgt tctgttttcg agttgtactg 1141 atttttcctc agatagtctt gtgggactat cattatataa gtaacctcat tacttatcag 1201 ttccttaaca gtgcctccta aaagtaatcc ttgtttgagc tttatggaaa gttatttatt 1261 taggaaagtc tggcttcttt cagcttagat tttcagtgtg atgtttctat tagaataaag 1321 ggcacagaca atccttcctt tgagaacaga atccacattg gcaccatagt cagtggcttt 1381 ttggaattag taactggaga atgaactggt attatgaaat ttttaaaggt tttataccct 1441 agataggcaa ttttgcaatc acaaaataat acattattga ttgaatataa acttaaaatt 1501 ccatccttga tcaaaggaac cacttttacc tcaaaccaag gtgatcacag aaccaagatg 1561 tacaactatg attgtttcat caccatttac caagttcctg acataatctt tgccagtaag 1621 catttaatat ttactgaaca aatgaactac tttttatgct aagttctcaa gttaatacac 1681 caggtttgta cttggttcac catagcttta attctattaa gtatatttaa aaaattgact 1741 taaaacacta caaatatagt ggacactgtt agcttcatct ctggctggta aattttgcta 1801 acctggttac gtgaatagga aataagctat gaagtttctc ctctagacaa ggtttttgtc 1861 acatagccct ggctggcctc tgcctcccaa gtgttgggaa ctaaagataa gcaataccat 1921 gccaggtgga aggcttctta ctgtgtttgt gtgaaattat gtgtaccagt gcaggtgcct 1981 gtggaggcta gaagctggtg ttaattggca gtctatgagc tatctggtgt gggtgatggg 2041 atgagaagtc aagtcctcct ctagagcagc ctgggatcca ttgccacctt tcacttagtt 2101 tgtttggatg gtttttgcat caggataaag ttcttgctca tcagttgtga agatgttagc 2161 cacttaagac accagtttca gcccaagttc ttcctgcctg ccttctgtga cccacgtaaa 2221 gcaatacatg cttttcaaga aagacctctc agtcctgtgt gctcagtacc agttttctag 2281 gctctttagg agccattcag tgttcactat gtacatcttt ctagagaagc catttctcaa 2341 aatttaaaac tttctaatct tacctattcc taaaattttg aaacactagc ataaatctta 2401 tttgagattt ttttgggggt gatggtggtc ctattttcag gactagatca ggatgtagct 2461 cagtgagaac actaacctag gagtttgaaa ctaaatttct gggttaaaag gcccagatgg 2521 ctggcatctt ctgtatttaa ttgataaggg ataagtatta gtcaggtgaa atcaatgtaa 2581 catggagtta ttatatacat cttccagaag ttggtctagg ttcattttgt atgaccaaat 2641 cattaagagt ccaaattgtc acactcctaa caatgtacaa gttcctccaa cttacctcaa 2701 agtttgaatt taatcacact tgtcagcata acaagccagc ctccctccct ccctccctcc 2761 ctgacaaaag ggatgcccaa ttccaactgt gaagacccaa acttgtcagt ctttcaaaag 2821 gatgtttgaa gtgtaagtgc atgcccccct ctttggttca caccacaaat gaacagtgcc 2881 aaggcactcc ccactttttc tttcaccatt agggcaaatg gcaacataac tgttctagac 2941 aaaccaatag attaaaaagt cattccacag tttgactatt taggacatag gtttattgcc 3001 aaactttcac ataactaaaa caagcacagt gacaagtcaa gccacatttg taagcttcat 3061 ccatttgtaa catttatgag ttatagatta gagtttatat ctgtggggaa aatttttaat 3121 ataacaagct gttatattgc tcaaaagaac cactgagtat ttcttttcat ctgccagata 3181 ttcagcaatg acaactatat ggttttaaat tttctaagct attacacttt ccttcaacta 3241 atacagcaag tagcttattt cccttctgaa atacttcagc tttttaattt ctagttcaaa 3301 gcttctgaca gttttgcttt ctaaaacacg aacaagtctg catttaaaat gttcaataac 3361 attcaaaaca atctgacact cattgaaatg actcctttca aaatgactac acaatattgc 3421 tcgcaccaac acaaaagtta gtatgttgca aaagacatgc tatattaaac tctctaaaga 3481 cagtatacat ttaagaaatg ttctggacaa acacttctac atgtcagttt tatatatatt 3541 ataacacaaa cggtgttaat tgaataatta agcattttaa aagcatgaaa ctacaaccat 3601 tgttcataaa taagttctaa cgttgtggtt ttataatttg gaactttggg gaaatgttca 3661 aaacatttta tgacaaacac ctttatcctc acatacaaag aaagctacaa tacttaaaat 3721 ggtaattgca taacacattg catggattat acaaggtgta acttaagagt ttaattcata 3781 cagaacacat atgttctacc tacactacat tagaaaaatc caaactgcat agcaacattt 3841 aacacagtga cattactgtc actgaccatc atgcctctgc ttgcttctga gaacataaat 3901 ggcaacatct tgggattatg aacatcatgt tgatctcgaa gacctgttgc tgctcagaca 3961 gcagaaatat agccttaaac aagcactgtc ctgtcctcaa ggaatgatct aaaggaggaa 4021 aaacaataat tacacaatta atattcttaa ttaagacttt aagagccatg gcaatgttca 4081 agcaggacca aatgtggtgc agtgttgagg gcaatctggg aaggttcctt aagaagttat 4141 gttctgagac cattctcagt ggctcaacaa cacttggtca aaaattttaa ttctcccctc 4201 agagaaatgg agtagttact ccactttcaa gttccttata agcttaccat caaccttata 4261 gtacactcta gatgtcctga aatatttcta tcagaacaag gtagtataaa gctggtaggt 4321 atacaaaacg ctagactagt ttctatccct gacccttaat ctgctagtat atccgtagga 4381 agttgcttaa gtgccactag taccaacagc ctctgttaga gtgaacagta gattttaatg 4441 aagggccaat aaagaccact taagactact gactgaatta aaaatccacc cagtggagtt 4501 agtattggtt atagttatat aacaaaatat ccaacttagc caggcgtggt ggtgcacgcc 4561 tttaatcccg gcactcagga ggcagaggca ggcggatttc tgagttcgag gccagcctgg 4621 tctacaaagt gagttccagg acagccaggg ctatacagag aaaccctgtc tcgaaaaacc 4681 aaaaaaaaaa aaaaaaagga aaaaaaatat ggcttgaaaa catgctattg ttgtcaaaag 4741 gagatccaaa caagtatttc tttgaacaaa aattatgtag aaaacagggt gataaagaac 4801 aagggctttt taaaaaaggc cagcattatt tgagatatac aagtgactcc aattcaagcc 4861 taaaggccac tcaatgctca ctaacagtgc taatcagaat caattcctta gcccagtcaa 4921 aaatagtcct gaatatcttc tggataaaca tgtcctccaa catataaact agtatacact 4981 aacaaaacgt ctcaacttca aggtgaaatg cttgactcct agacttgtga cccagcaaag 5041 tgctctatag gtagggttac taggtcagag agtcttgcct gcaaaccaca attatacaat 5101 tccaccaaat gtaagacatg taaaaatgac ttatttagct caggttttga aaattaaaaa 5161 tcaaacccac aaagacctaa ctttcacatt aagtacaaat gtttaacata tataacatgt 5221 tttgccttga tatatgaaat catatgacat cacctgacag gaaacttaag tttatttgaa 5281 caacaatcag cctaaggtag gcctagcaca tgatcaggat agtgcaggga aacccaaaga 5341 agtgcttctg agtataaacc aagagacctc gaaatagcag ctttgttctg tatctcatgg 5401 gctttaacta tcacttgaaa acaatttcac aaatcacaaa accaacattt gtttagtttc 5461 caactctgcc tattcaccgg agaaatccat gtcccaattt gatgggggaa aacttgaatg 5521 aaatattatt tggaatattt taatatccct gtgtatatgc agatggttta aagacaggta 5581 accttaactg tagtttcttg gttgtctgcc ttgttttttg aaatatgctc tcaccaggag 5641 cctgccaagt aactactctt gtgtgctcag caagtcctag ggatccctta agcatgctct 5701 aacaggcctg gctttttttt ttaaaaagta ggttgcggtg ataataattc aagttttcat 5761 cacttgaaac acattttacc aactatcacc caagctccct tagtgtgtcc cctataaaag 5821 aatctgacct gcaagttcca aaagtattac tacattttaa ccttagtatt caggagacaa 5881 aagcaggcag aactttagtc tacatatctt gttgcagatc aggcagagat acatagtgag 5941 accctgcctc aggaaaagct atcattttta tattcacagt aagcaaatta acattaaaag 6001 tcagaaattc ttaatttgac tttggctgtg aagaatttgg attctctgga tttgaaacta 6061 gaaaatctga ttaagcgatg caacagtttc tgtatactaa aactctaaga cccttggttc 6121 taaagatacc acatttaaaa aaaaagttaa gtataagaca aaaagcctta atagctcttt 6181 tataggggag ggactcattt aatattagtc cacctcactc ctcataacta ttttatgagg 6241 tttattatat aacccatttt ttaaaaaaaa aattaaagga caattttagt gtttgaaaga 6301 tttcccaacc ccaccctgga aatcaggctg caaatctcag cagctgccct tagaaggcaa 6361 aaagccatgc catttaagcc atgggaagtt agtagcaaac aagagaccac atggcaggaa 6421 ggaggcttta aagaaagccc acagtgtcaa aagacccaac caacagcaga gacagaaaaa 6481 ataataccca aatctcatca gaaaggtaga cggatttagc cacatccata gtggctcatt 6541 agggaatgct tcaattataa ttttatgaaa tctcagagcc cccctcccca acagggtttc 6601 tgtgtgtatt cctggctatc ctagatagaa cttgatgtgt agaccaggct gggctcaaac 6661 tcagagatct gcctgtctct gcctccagag tgctgggatt aaaggcataa aggcaagcac 6721 caccactggc tggctaaact ctggccctac atttttgaaa tccaaaatta tcattacttg 6781 ctttcatgtt tagtaatggc tgcagactta gctttcagct ttgtatagat gaagcacaaa 6841 agttaagtaa agctagcagt ttaaacaaat aggatactag aagttcaatt tttatttctt 6901 tattgcttag tgtaagagaa aagacattaa agtctgcatt ggtcttctgt atcccacaag 6961 tctgcagtta tggctcctct gtccacagtt acacttcaaa taaaaattag ttccttggta 7021 acatttccag tgcttaaaga gtaatttgat tattggctac catattggaa caaacacaaa 7081 gtatttcatt acttcagtaa attctactgg agacagtatt ctctttaaaa aaaaaaaaaa 7141 gaagaagaag gaggatttat ttatttaatg tgaatacact tgtggtcttc agacacacca 7201 gaagagggca tcagatccca ttacagatgg ttgtgagcca ccatgtggtt gctgggaatt 7261 gaactcagga cttctggaag agcagtctgt gctcttgaac tccgagccac ctctccagcc 7321 cctggatagt attattatag agcacaagca cacacaacac aacaacctaa aaaccaagct 7381 tcacaaatac atttgttgct taatcatttt acttgtgttt ggctacaagt caagcaaaat 7441 tataggtcct gaagaagctt ggcaaaatca catttagacc agcaataacg tgtagaatgc 7501 catgagtcaa gccagtccaa gagaaagcac tgcttaaata gaaaaaatac aagctcacaa 7561 gaccttaggt caggaaagac aacaaacacc tgaactttgc attccaaaag gaaccacctt 7621 ttacagatgt gtacaatgac aaaattttag taagcagtaa tcaataccat gtggctcaat 7681 aatgaaatat accttttaat gtcttaaaat atcagcctgg caatatgtaa gatacatcag 7741 gtaatattgg gggaggagac atccacctgg aaaccattaa tggttaatag ctcagtttat 7801 aaatggagaa aaaggagaga ggcattcatg ggagtggaaa gttaagcttt aggttggatt 7861 ctcaatacat ctattgttgt aaaactccct ggactgagaa taggcccaaa tgtggaacac 7921 cacctgacgg gagaggtgat agacactgaa aattaaggat caaggcaaag gatcaacaaa 7981 aagtgtacta aggagttata aaagaactgc agtgttgagg ccccagctac agcctcgatt 8041 tgtggtgtat gtaactaatc tgtctggttt catgagtcat cagacttcta agatcaggaa 8101 agggaaaatg ccaatgctct gtctaggggt tggataagcc agtataataa atgaaaatgg 8161 ggctaaaatg agtgttctaa aatacctttt gataaggctg cagaaggagc gggagaaatg 8221 gatatgaagt actgggctct ttaaaaatga ttaaaattct gcttacatag tctaactcgc 8281 gacactgtaa tttcatactg tagtaaggat ctcaagcagg agagtataaa actcgggtga 8341 gcatgtcttt aatctacctg gtttgtaccg tacaccactg agtccgccgt cgtcgacaag 8401 ccggcccatg attacgcgaa tttaaatcgg atccatatgg cgcgccggat tcgacattga 8461 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg 8521 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 8581 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 8641 tgacgtcaat gggtggacta tttacggtaa actgcccact tggcagtaca tcaagtgtat 8701 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 8761 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 8821 gctattacca tgggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc 8881 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc 8941 gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga 9001 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg 9061 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg 9121 cgttgccttc gccccgtgcc ccgctccgcg ccgcctcgcg ccgcccgccc cggctctgac 9181 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt 9241 agcgcttggt ttaatgacgg ctcgtttctt ttctgtggct gcgtgaaagc cttaaagggc 9301 tccgggaggg ccctttgtgc gggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc 9361 gtggggagcg ccgcgtgcgg cccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg 9421 cggggctttg tgcgctccgc gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg 9481 gtgcgggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga 9541 gcagggggtg tgggcgcggc ggtcgggctg taaccccccc ctgcaccccc ctccccgagt 9601 tgctgagcac ggcccggctt cgggtgcggg gctccgtgcg gggcgtggcg cggggctcgc 9661 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc 9721 cggggagggc tcgggggagg ggcgcggcgg ccccggagcg ccggcggctg tcgaggcgcg 9781 gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg 9841 tcccaaatct ggcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg 9901 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc 9961 gcgccgccgt ccccttctcc atctccagcc tcggggctgc cgcaggggga cggctgcctt 10021 cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc 10081 tctgctaacc atgttcatgc cttcttcttt ttcctacaga tccttaatta aggcaacgtg 10141 ctggttattg tgctgtctca tcattttggc aaagaattct gagccgccac catgggcaaa 10201 cctattccta accctctcct cgggctcgat tcaactggta gcggcaaacc tattcctaac 10261 cccctcctcg ggctggacag caccggatcc ggcaagccaa tccccaaccc tctgctggga 10321 ctggattcta cagtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 10381 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 10441 gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc 10501 tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg ctaccccgac 10561 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccagatttac 10621 gacccctgtg tgggattata gtgtgggatt atcggggtgt aatcttcttc aaggacgacg 10681 gcaactacaa gacccgcgcc gaggtcaagt tcgagggcga caccctggtc aaccgcatcg 10741 agctcaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 10801 actacaacag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca 10861 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc 10921 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctcagctacc 10981 agtccgccct cagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 11041 tcaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaccg gaagcggagc 11101 taccaacttc agcctgctca agcaggctgg agacgtggag gagaaccctg gacctcagat 11161 ggtgggtgag gatagcgtgc tgatcaccga gaacatgcac atgaaactgt acatggaggg 11221 caccgtgaac gaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg 11281 cacccagacc atgaagatca aggtggtcga gggcggccct ctccccttcg ccttcgacat 11341 cctggctacc agcttcatgt acggcagcaa aacctttatc aaccacaccc agggcatccc 11401 cgacttcttt aagcagtcct tccctgaggg cttcacatgg gagaggatca ccacatacga 11461 agacgggggc gtgctgaccg ctacccagga caccagcctc cagaacggct gcctcatcta 11521 caacgtcaag atcaacgggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac 11581 actcggctgg gaggccagca ccgagatgct gtaccccgct gacagcggcc tgagaggcca 11641 tgcccagatg gccctgaagc tcgtgggcgg gggctacctg cactgctccc tcaagaccac 11701 atacagatcc aagaaacccg ctaagaacct caagatgccc ggcttctact tcgtggaccg 11761 gagactggaa agaatcaagg aggccgacaa agagacctac gtcgagcagc acgagatggc 11821 tgtggccaga tactgcgacc tgcctagcaa actggggcac agcgccacga acttctctct 11881 gttaaagcaa gcaggagatg ttgaagaaaa ccccgggcct atggaagatg ccaaaaacat 11941 taagaagggc ccagcgccgt tctacccact cgaagacggg accgccggcg agcagctgca 12001 caaagccatg aagcgctacg ccctggtgcc cggcgccatc gcctttaccg acgcacatat 12061 tcaggtggac gttacctacg ccgagtactt cgagatgagc gttcggctgg cagaagctat 12121 gaggcgctat gggctgaata caaaccatcg gatcgtggtg tgcagcgaga atagctcgca 12181 gttcttcatg cccgtgttgg gtgccctgtt catcggtgtg gctgtggccc cagctaacga 12241 catctacaac gagcgcgagc tgctgaacag catgggcatc agccagccca ccgtcgtatt 12301 cgtgagcaag aaagggctgc gaaaggtcct caacgtgcaa aagaagctac cgatcatacg 12361 aaagatcatc atcatggata gcaagaccga ctaccagggc ttccaaagca tgtacacctt 12421 cgtgacttcc catttgccac ccagcttcaa cgagtacgac ttcgtgcccg agagcttcga 12481 ccgggacaaa accatcgccc tgatcatgaa cagtagtggt agtacaggat tacccaaggg 12541 cgtagcccta ccgcaccgca ccgcttgtgt ccgattcagt catgcccgcg accccatctt 12601 cggctaccag aacatccccg acaccgctat cctcagcgtg gtgccatttc accacggctt 12661 cggcatgttc accacgctgg gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg 12721 cttcgaggag gagctattct tgcgcagctt gcaagactat aagattcaat ctgccctgct 12781 ggtgcccaca ctatttagct gcctcgctaa gagcactctc atcgacaagt acgacctaag 12841 cagcttgcgc gagatcgcca gcggcggggc gccgctcagc aaggaggtag gtgaggccgt 12901 ggccaaacgc ttccgcctac caggcatccg ccagggctat ggcctgacag aaacaaccaa 12961 cgccgtcatg atcacccccg agggggaccg taagcctggc tcagtaggca aggtggtgcc 13021 cttcttcgag gctaaggtgg tagacttggt caccggtaag acactgggtg tgaaccagcg 13081 cggtgagctg tgcgtccgtg gccccatgat catgagcggc tacgttaaca accccgaggc 13141 tacgaacgct ctcatcgaca aggacggctg gctgcacagc ggcgacatcg cctactggga 13201 cgaggacgag cacttcttca tcgtggaccg gctgaagagc ctgatcaaat acaagggcta 13261 ccaggtagcc ccagccgaac tggagggcat cctgctgcaa cacccctaca tcttcgacgc 13321 cggagtcgcc ggcctgcccg acgacgatgc cggcgagctg cccgccgcag tcgtcgtgtt 13381 ggaacacggt aaaaccatga ccgagaaaga gatcgtggac tatgtggcca gccaggttac 13441 aaccgccaag aagctgcgcg gtggtgttgt gtttgtggat gaagtcccta gaggatcgac 13501 cggcaagtta gacgcccgca agatccgcga gattctcact aaggccaaga aggacggcaa 13561 gatcgccgtg gactacaagg atgacgatga caagggaagt gattacaagg atgacgatga 13621 taaggggtct gattacaaag atgatgatga taagtaagcg atcgcacgcg taaatgattg 13681 cagatccact agttctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc 13741 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt 13801 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct 13861 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc 13921 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgagatc 13981 cactagttct agcctcgagg ctagagcggc cgcatatgaa ttcatagtac cggttgtaac 14041 gcgtgctagc agagctcgag tactcagatc taagcttgga tgatcctgac gacggagacc 14101 gcggtggttg accagacaaa ccggtttgtt ctagaaagac tggagttgca gatcacgagg 14161 gaagaggggg aagggattct cccaggccca gggcggtcct cagaagccag gaggcagcag 14221 agaactccca gaaaggtatt gcaacactcc cctcccccct ccggagaagg gtgcggcctt 14281 ctccccgcct actccactgc agctccctta ctgataacaa ctcagagcga ctttgggaga 14341 gcaagtgctt cctgcctcca aaacagccca actgagccct cgctccttcc ctccactccc 14401 cggagtgcgc gatggaggtc tggctcagca cgcccctctt gaggcaactc aagtcggaaa 14461 cgtgcttgca cccgccccgc agccgctcag ccctactgcc cgtccccgcc cccagcgcgc 14521 gcttcctgcc acgttgcgca ggggcgcgcg gccagactct gcggcgcggg gccgagggga 14581 gggccggaac ctgggagcgc ctcctcgccg cccccgctgg ccggcggatg gactcaactt 14641 gcacgaacac gagccaatgg caagggccag ttttctgggc cccgagagcc aatcagacga 14701 cgaggcccgg ccggcggcgg gtaaaacgac tcccccagag gaaggggagg gtgggcggcc 14761 gctcgcgcgc gagctacttt cgctgaccct ccctcccctc ccccgccccg ccagaggccg 14821 accgcgcccg cacgtccagc tcgcctcacc ccacctacct cccgccccac ccagtgggca 14881 gagcgaggct gccggcggct gcgcactccg gctgccgtta actgacaggc gccttacgcc 14941 aaccaaaaca cgccatttgt gttttcacac acggcgggag gaaaagaagc caatcagcga 15001 cgagacgtcg gccggaagcg ctcctccgct gccccccccc ccccgagcca tggccgcgtc 15061 cggtggagac ttttccgctc ccttctccct ccccctccgg ttgctgcagg gcggaccgca 15121 ttcctgccca ccacccgctt gccccttcca gcgtcacgac tcgtacccgg ctgtctcaca 15181 gaacggctcc accacgctcg gagggcctgc cgcggccgcc catccccgca aacgcaccaa 15241 gccgccctcc cgccagagtc ccgatcccct acctagccga ggctctctga ggagccggag 15301 ccccaaagca cagcctcttc tttaggcggc cccggcagcc tccgctgatt ggcggcgcac 15361 cggccaataa gtgtggggcg gtgggcggag aggccaatgg cgcggcggga gggggcgtgt 15421 cccgcgtgcc cctggcgccg gcactgggaa tccccgtgcg gtcagtggcg tttccgctcg 15481 ggcagcgggc tgagtgagct gccgccgctg ccgccgctgc cgccgccggg ggaggggcgg 15541 ccgccgcccg cctgcgctca gagactcacg cagccctagt cccgccagtc cgccaacaca 15601 gtagtgccgg ccccctccgc ccctggccct ccccctcccc gcctcgggct cgctccgcct 15661 tttctgcccc ccacccccac ctcacgggta cgggccattc ccggccagga aacgccgtgg 15721 cgccgcgttg ggcctaactc gagtctcgct gcctcccggg agtgccgtgc gccgcagccc 15781 gggcccaggc cccggcagcg cctgggtcaa ggtaagggtc caacagaaaa gagacaccga 15841 accacacgac cgggccccgg gggaaagaag ggtcacctca gtctccctgc gttccctctc 15901 ctcggggtcg tgggcctggg cctccccagc ctggtagcag gaagatgctt gcccttttcg 15961 tcttctcagc tacctttaca caccattgca ccgctcttgc ccagagagaa aggctctcct 16021 tcatctagtc gaccccacta cctttttaat gtcttccctg ggtcaggact cttcccctcc 16081 ccctactctg gtctcccctt tttgcctggg tattgcctac tccacgttta tacccttttc 16141 aggagaggcc tcccaaccct gctctcaaaa tacacatact tttttttctg tccctgagcc 16201 ccccacctcc cctgttcttg cggccttgtg acaactctgg tcgctcgtgg gggcccagtc 16261 ctcccctcca taatcttcct gaacgcctct cctctggttt tccagttcct atctcagatg 16321 gctgctgctt ttcccacacc aaagacatta ccttcgccac ccccacctca cattcttgga 16381 ctccctgtgg cgtatgcccc agtatccttg cgatatccct gttgaatttg ta //