LOCUS Exported 14431 bp DNA linear SYN 12-OCT-2022 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 14431) AUTHORS . TITLE Direct Submission JOURNAL Exported Oct 12, 2022 from SnapGene 6.0.5 https://www.snapgene.com FEATURES Location/Qualifiers source 1..14431 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature join(1..8359,12128..13431) /label=Gt(ROSA)26Sor - MGI:104735 /note="Gt(ROSA)26Sor - MGI:104735" /note="This feature has 2 segments: 1: 1 .. 8359 / #ff6666 2: 12128 .. 13431 / #ff6666" polyA_signal 2992..2996 /label=PolyA Signal /note="color: #a6acb3" primer_bind 4141..4162 /label=1912 /note="color: black; sequence: GTTCTGAGACCATTCTCAGTGG" primer_bind 4624..4647 /label=1278 /note="color: black; sequence: ACAAAGTGAGTTCCAGGACAGCCA" primer_bind complement(4624..4647) /label=1101 /note="color: black; sequence: TGGCTGTCCTGGAACTCACTTTGT" primer_bind complement(4681..4697) /label=2579 /note="color: black; sequence: TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT TAGGAAAGGACAGTGGGAGTGGC; added: 2019-10-24" primer_bind complement(4681..4696) /label=2579 /note="color: black; sequence: TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT TAGGAAAGGACAGTGGGAGTGGC; added: 2019-10-24" primer_bind complement(4681..4695) /label=2579 /note="color: black; sequence: TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT TAGGAAAGGACAGTGGGAGTGGC; added: 2019-10-24" primer_bind 5015..5038 /label=1107 /note="color: black; sequence: ACTCCTAGACTTGTGACCCAGCAA" primer_bind complement(5065..5088) /label=1100 /note="color: black; sequence: TGGTTTGCAGGCAAGACTCTCTGA" primer_bind 5313..5333 /label=1329 /note="color: black; sequence: ATCAGGATAGTGCAGGGAAAC" primer_bind 5462..5481 /label=1327 /note="color: black; sequence: AACTCTGCCTATTCACCGGA" primer_bind 5473..5496 /label=1106 /note="color: black; sequence: TTCACCGGAGAAATCCATGTCCCA" primer_bind complement(5778..5801) /label=1099 /note="color: black; sequence: AAGGGAGCTTGGGTGATAGTTGGT" primer_bind complement(5823..5842) /label=1326 /note="color: black; sequence: TTTGGAACTTGCAGGTCAGA" primer_bind complement(6203..6224) /label=1328 /note="color: black; sequence: TGAGGAGTGAGGTGGACTAATA" primer_bind 6952..6975 /label=1104 /note="color: black; sequence: TCCCACAAGTCTGCAGTTATGGCT" primer_bind complement(7188..7211) /label=1097 /note="color: black; sequence: ATGCCCTCTTCTGGTGTGTCTGAA" primer_bind 7507..7527 /label=2680 /note="color: black; sequence: TCAAGCCAGTCCAAGAGAAAG; added: 2019-12-04" primer_bind 7507..7527 /label=2707 /note="color: black; sequence: TCAAGCCAGTCCAAGAGAAAG; added: 2019-12-04" primer_bind 7816..7839 /label=1103 /note="color: black; sequence: AGAGAGGCATTCATGGGAGTGGAA" primer_bind complement(7816..7839) /label=1096 /note="color: black; sequence: TTCCACTCCCATGAATGCCTCTCT" primer_bind 7957..7976 /label=Primer_KJS_01_Fwd /note="color: black; sequence: GGATCAAGGCAAAGGATCAA; added: 2020-06-25" primer_bind 8030..8049 /label=1703 /note="color: black; sequence: CAGCCTCGATTTGTGGTGTA" primer_bind 8107..8127 /label=1814 /note="color: black; sequence: AATGCCAATGCTCTGTCTAGG" primer_bind 8188..8211 /label=1068 /note="color: black; sequence: TTTGATAAGGCTGCAGAAGGAGCG" primer_bind 8206..8226 /label=2488 /note="color: black; sequence: GGAGCGGGAGAAATGGATATG; added: 2019-12-04" primer_bind 8270..8291 /label=2718 /note="color: black; sequence: GTCTAACTCGCGACACTGTAAT; added: 2019-12-04" primer_bind 8327..8346 /label=1140 /note="color: black; sequence: TAAAACTCGGGTGAGCATGT" primer_bind complement(8360..8387) /label=1927 /note="color: black; sequence: GGTTTGTCTGGTCAACCACCGCGGTCTCAGTGGTGTACGGTACAAACC" misc_feature 8360..8384 /label=Bxb1 MUT attP /note="color: #ff0000" primer_bind 8360..8382 /label=2162 /note="color: black; sequence: GGTTTGTACCGTACACCACTgag" primer_bind 8360..8382 /label=1928 /note="color: black; sequence: GGTTTGTACCGTACACCACTGAGACCGCGGTGGTTGACCAGACAAACC" primer_bind complement(8371..8388) /label=2493 /note="color: black; sequence: gGCGGaCTCAGTGGTGTA; added: 2019-12-04" primer_bind complement(8371..8387) /label=1956 /note="color: black; sequence: CGCGGTCTCAGTGGTGTA" misc_feature 8383..8384 /label=GA /note="color: #a6acb3" misc_feature complement(8385..8402) /label=Bxb1 attB(BB') SITE (GA) /note="color: #ff6600; direction: LEFT" enhancer 8454..8833 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" /note="color: #ffffff" primer_bind complement(8526..8546) /label=2361* /note="color: black; sequence: CCGTAAGTTATGTAACGCGGA; added: 2019-10-24" primer_bind complement(8777..8798) /label=2283 /note="color: black; sequence: GCCAAGTAGGAAAGTCCCATAA; added: 2019-10-24" promoter 8835..9112 /label=chicken beta-actin promoter /note="color: #ffffff; direction: RIGHT" intron 9113..10130 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" /note="color: #------" primer_bind 10088..10107 /label=2049 /note="color: black; sequence: AGCCTCTGCTAACCATGTTC; added: 2019-10-24" regulatory 10143..10152 /label=Kozak sequence /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /note="color: #a6acb3" /regulatory_class="other" misc_feature 10152..10433 /label=Venus /note="Is in the +3 reading frame... no stop in 26-1 in that frame." /note="color: #008000" primer_bind complement(10176..10186) /label=2161 /note="color: black; sequence: GGTTTGTCTGGTCAACCACCgcggt" primer_bind 10213..10232 /label=Primer_KJS_06_Fwd /note="color: black; sequence: ACGTAAACGGCCACAAGTTC; added: 2020-06-25" misc_feature complement(10355..10374) /label=Guide 26-52 /note="color: #800000; direction: LEFT" primer_bind complement(10380..10399) /label=Primer_KJS_05_Rev /note="color: black; sequence: AAGTCGTGCTGCTTCATGTG; added: 2020-06-25" primer_bind 10391..10417 /label=1615 /note="color: black; sequence: GCACGACTTCTTCAAGTCCGCCATGCC; added: 2019-10-24" misc_feature 10434..10453 /label=Guide 26-1 /note="color: #800000; direction: RIGHT" primer_bind 10434..10447 /label=2719 /note="color: black; sequence: GATCTGCAACTCCAGTCTTTCT; added: 2019-12-04" misc_feature complement(10440..10459) /label=TLR7-g2-REV /note="color: #800000; direction: LEFT" misc_feature 10464..10890 /label=Venus /note="color: #008000" primer_bind 10477..10495 /label=1594 /note="color: black; sequence: ACGACGGCAACTACAAGAC; added: 2019-10-24" primer_bind 10856..10875 /label=2701 /note="color: black; sequence: GATCACTCTCGGCATGGACG; added: 2019-10-24" CDS 10892..10948 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /note="color: #cc99b2 Cleavage site after base 10945" /translation="ATNFSLLKQAGDVEENPGP" CDS 10949..11656 /codon_start=1 /product="improved version of the oligomeric far-red fluorescent protein TurboFP635/Katushka (Luker et al., 2015) In the +2 reading frame" /label=Katushka2S /note="mammalian codon-optimized" /note="color: #a0003c" /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV ARYCDLPSKLGHS" primer_bind 10967..10986 /label=2830 Katushka 2S Rev /note="color: black; sequence: GTGCTGATCACCGAGAACAT; added: 2019-12-04" primer_bind complement(11618..11636) /label=2829 Katushka 2S Fwd /note="color: black; sequence: TAGGCAGGTCGCAGTATCT; added: 2019-12-04" primer_bind complement(11712..11729) /label=1432 /note="color: black; sequence: TAGAAGGCACAGTCGAGG; added: 2019-10-24" polyA_signal 11718..11942 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" /note="color: #a6acb3" primer_bind complement(11722..11742) /label=2113 /note="color: black; sequence: GATGGCTGGCAACTAGAAGGC; added: 2019-10-24" primer_bind complement(11725..11744) /label=2050 /note="color: black; sequence: CAGATGGCTGGCAACTAGAA; added: 2019-10-24" primer_bind complement(11786..11814) /label=2579 /note="color: black; sequence: TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT TAGGAAAGGACAGTGGGAGTGGC; added: 2019-10-24" primer_bind 11889..11910 /label=1591 /note="color: black; sequence: GGGAGGATTGGGAAGACAATAG; added: 2019-10-24" primer_bind 11889..11910 /label=2284 /note="color: black; sequence: GGGAGGATTGGGAAGACAATAG; added: 2019-10-24" primer_bind complement(11893..11913) /label=2632 /note="color: black; sequence: ttctACCGGTCTGCTATTGTCTTCCCAATCC; added: 2019-10-24" primer_bind complement(11933..11953) /label=2358 /note="color: black; sequence: CGCCTCAGAAGCCATAGAGCC; added: 2019-10-24" primer_bind complement(11965..11983) /label=2347 /note="color: black; sequence: AGTGGATCTCGAGCCCCAG; added: 2019-10-24" primer_bind 11984..12003 /label=2344 /note="color: black; sequence: AGTTCTAGCCTCGAGGCTAG; added: 2019-12-04" primer_bind complement(11988..12004) /label=2344 /note="color: black; sequence: AGTTCTAGCCTCGAGGCTAG; added: 2019-12-04" primer_bind 12017..12082 /label=2665 /note="color: black; sequence: ttaaTCGGATCCgatatcgaattcATAGTACCGGTTGTAACGCGTGCTAGCAGAGCTCG AGTACTCAGATCTAAGCTTGGATGA; added: 2019-10-24" misc_feature complement(12079..12096) /label=BxbI attB(BB') SITE (GT) /label=BxbI AttB /note="/ApEinfo_fwdcolor=#ffee30 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /ApEinfo_revcolor=green" /note="color: #00ff00; direction: LEFT" primer_bind 12079..12096 /label=2164 /note="color: black; sequence: atgatcctgacgacgGAG; added: 2019-10-24" primer_bind 12094..12121 /label=1928 /note="color: black; sequence: GGTTTGTACCGTACACCACTGAGACCGCGGTGGTTGACCAGACAAACC" primer_bind complement(12094..12121) /label=1927 /note="color: black; sequence: GGTTTGTCTGGTCAACCACCGCGGTCTCAGTGGTGTACGGTACAAACC" misc_feature 12097..12121 /label=Bxb1 AttP Site /note="Bxb1 AttP Site" /note="color: #ff0000" primer_bind complement(12097..12121) /label=2161 /note="color: black; sequence: GGTTTGTCTGGTCAACCACCgcggt" misc_feature 12097..12098 /label=GT /note="color: #a6acb3" misc_feature 12122..12127 /label=INDEL /note="GET 314 INS" /note="color: #ffff00" primer_bind complement(12132..12153) /label=2719 /note="color: black; sequence: GATCTGCAACTCCAGTCTTTCT; added: 2019-12-04" primer_bind complement(12196..12220) /label=1310 /note="color: black; sequence: TCTGCTGCCTCCTGGCTTCTGAGGA" primer_bind complement(12211..12233) /label=1076 /note="color: black; sequence: TTTCTGGGAGTTCTCTGCTGCCT" primer_bind complement(12231..12251) /label=1141 /note="color: black; sequence: GGGGAGTGTTGCAATACCTTT" primer_bind 12296..12317 /label=2819 /note="color: black; sequence: CTGCAGCTCCCTTACTGATAAC; added: 2019-12-04" primer_bind complement(12309..12330) /label=1699 /note="color: black; sequence: GTCGCTCTGAGTTGTTATCAGT" primer_bind complement(12316..12339) /label=1207 /note="color: black; sequence: TCTCCCAAAGTCGCTCTGAGTTGT" primer_bind complement(12328..12347) /label=Primer_KJS_07_Rev /note="color: black; sequence: GCACTTGCTCTCCCAAAGTC; added: 2020-06-25" primer_bind complement(12447..12466) /label=2711 /note="color: black; sequence: AAGCACGTTTCCGACTTGAg; added: 2019-12-04" primer_bind complement(12448..12466) /label=1813 /note="color: black; sequence: AAGCACGTTTCCGACTTGA" primer_bind complement(12635..12658) /label=1069 /note="color: black; sequence: CATTGGCTCGTGTTCGTGCAAGTT" primer_bind 12769..12787 /label=2047 /note="color: black; sequence: CGAGCTACTTTCGCTGACC" primer_bind complement(12983..13001) /label=2705 /note="color: black; sequence: CGTCGCTGATTGGCTTCTt; added: 2019-12-04" primer_bind 13055..13071 /label=2051 /note="color: black; sequence: GCGTCCGGTGGAGACTT" primer_bind complement(13301..13321) /label=1209 /note="color: black; sequence: AAGAAGAGGCTGTGCTTTGGG" primer_bind complement(13805..13825) /label=1911 /note="color: black; sequence: CTGTTGGACCCTTACCTTGAC" primer_bind complement(13975..13998) /label=1211 /note="color: black; sequence: CAAGAGCGGTGCAATGGTGTGTAA" ORIGIN 1 tttgtacaca atacatttat ttggtgccct caacagaaga ggatgttgga tggaccccct 61 agaactggag ttatagttgt aagccacaat gtatcagaaa ctgaacccag atcctctcta 121 aaaacagcaa gtgattttct gagtcaactt tccaacccac cacccatagc atttaagtgg 181 gaagatttta ggttgtttta gaatcagact ggtagcctaa tgacttatgt tttttgtgtg 241 tgccaggcct tatctggaat gggacatgtg tggcgaaagg tccatgtagt ctgggtgaac 301 atcgggggcc ttcatgctgc agtttatctt ctaaagcgca ctgctcaagc ctttgttcat 361 ccttcagatc aagatgaagg aagagaccct ccttggtaaa gaaaagagtg aagacaactt 421 attaatattt gtagttccta acactggaaa tatcagcata aagaacttgc tttgggagaa 481 aaatagcaga aaagtaactt acagtacagg ttacactgct tgaccactcc agaatgcttg 541 atttctagca aggtgattgt aatggtattt cttaagaagc ctacactgct tggcttctaa 601 gtgtcagaac actttaggcc atattctatt gcttgtgcaa cctactgttt tatggtctaa 661 attctttgta tcatctcaga agcagaagca tcccttaaga tctacagttt tatcatctgc 721 cttaaaataa atatacaacc taaacagagc aaagatgctt gtgtgactat tataatatta 781 ctaagtagta gaaacaccag tagatgtatg gaaccaacaa gcctaattaa agaaatttct 841 cttttagtgt atggaagcgt tattttccaa agccctcccc acccccaaag tgcctgtcag 901 tcttaggggg tagagggagc taatgtatct ttaaatgaaa atgtaaactg tacactgtac 961 tgcttactca ggacaaagat ttaccaaggt acctcaggag agttaaaatc acccacctcc 1021 ctgtttgtat acaatatgta ggtctggggc cctcaacaga aaagggtgtt ggatagacct 1081 cctgacttgg gagccctgtt attcctgcct agctggttgt tctgttttcg agttgtactg 1141 atttttcctc agatagtctt gtgggactat cattatataa gtaacctcat tacttatcag 1201 ttccttaaca gtgcctccta aaagtaatcc ttgtttgagc tttatggaaa gttatttatt 1261 taggaaagtc tggcttcttt cagcttagat tttcagtgtg atgtttctat tagaataaag 1321 ggcacagaca atccttcctt tgagaacaga atccacattg gcaccatagt cagtggcttt 1381 ttggaattag taactggaga atgaactggt attatgaaat ttttaaaggt tttataccct 1441 agataggcaa ttttgcaatc acaaaataat acattattga ttgaatataa acttaaaatt 1501 ccatccttga tcaaaggaac cacttttacc tcaaaccaag gtgatcacag aaccaagatg 1561 tacaactatg attgtttcat caccatttac caagttcctg acataatctt tgccagtaag 1621 catttaatat ttactgaaca aatgaactac tttttatgct aagttctcaa gttaatacac 1681 caggtttgta cttggttcac catagcttta attctattaa gtatatttaa aaaattgact 1741 taaaacacta caaatatagt ggacactgtt agcttcatct ctggctggta aattttgcta 1801 acctggttac gtgaatagga aataagctat gaagtttctc ctctagacaa ggtttttgtc 1861 acatagccct ggctggcctc tgcctcccaa gtgttgggaa ctaaagataa gcaataccat 1921 gccaggtgga aggcttctta ctgtgtttgt gtgaaattat gtgtaccagt gcaggtgcct 1981 gtggaggcta gaagctggtg ttaattggca gtctatgagc tatctggtgt gggtgatggg 2041 atgagaagtc aagtcctcct ctagagcagc ctgggatcca ttgccacctt tcacttagtt 2101 tgtttggatg gtttttgcat caggataaag ttcttgctca tcagttgtga agatgttagc 2161 cacttaagac accagtttca gcccaagttc ttcctgcctg ccttctgtga cccacgtaaa 2221 gcaatacatg cttttcaaga aagacctctc agtcctgtgt gctcagtacc agttttctag 2281 gctctttagg agccattcag tgttcactat gtacatcttt ctagagaagc catttctcaa 2341 aatttaaaac tttctaatct tacctattcc taaaattttg aaacactagc ataaatctta 2401 tttgagattt ttttgggggt gatggtggtc ctattttcag gactagatca ggatgtagct 2461 cagtgagaac actaacctag gagtttgaaa ctaaatttct gggttaaaag gcccagatgg 2521 ctggcatctt ctgtatttaa ttgataaggg ataagtatta gtcaggtgaa atcaatgtaa 2581 catggagtta ttatatacat cttccagaag ttggtctagg ttcattttgt atgaccaaat 2641 cattaagagt ccaaattgtc acactcctaa caatgtacaa gttcctccaa cttacctcaa 2701 agtttgaatt taatcacact tgtcagcata acaagccagc ctccctccct ccctccctcc 2761 ctgacaaaag ggatgcccaa ttccaactgt gaagacccaa acttgtcagt ctttcaaaag 2821 gatgtttgaa gtgtaagtgc atgcccccct ctttggttca caccacaaat gaacagtgcc 2881 aaggcactcc ccactttttc tttcaccatt agggcaaatg gcaacataac tgttctagac 2941 aaaccaatag attaaaaagt cattccacag tttgactatt taggacatag gtttattgcc 3001 aaactttcac ataactaaaa caagcacagt gacaagtcaa gccacatttg taagcttcat 3061 ccatttgtaa catttatgag ttatagatta gagtttatat ctgtggggaa aatttttaat 3121 ataacaagct gttatattgc tcaaaagaac cactgagtat ttcttttcat ctgccagata 3181 ttcagcaatg acaactatat ggttttaaat tttctaagct attacacttt ccttcaacta 3241 atacagcaag tagcttattt cccttctgaa atacttcagc tttttaattt ctagttcaaa 3301 gcttctgaca gttttgcttt ctaaaacacg aacaagtctg catttaaaat gttcaataac 3361 attcaaaaca atctgacact cattgaaatg actcctttca aaatgactac acaatattgc 3421 tcgcaccaac acaaaagtta gtatgttgca aaagacatgc tatattaaac tctctaaaga 3481 cagtatacat ttaagaaatg ttctggacaa acacttctac atgtcagttt tatatatatt 3541 ataacacaaa cggtgttaat tgaataatta agcattttaa aagcatgaaa ctacaaccat 3601 tgttcataaa taagttctaa cgttgtggtt ttataatttg gaactttggg gaaatgttca 3661 aaacatttta tgacaaacac ctttatcctc acatacaaag aaagctacaa tacttaaaat 3721 ggtaattgca taacacattg catggattat acaaggtgta acttaagagt ttaattcata 3781 cagaacacat atgttctacc tacactacat tagaaaaatc caaactgcat agcaacattt 3841 aacacagtga cattactgtc actgaccatc atgcctctgc ttgcttctga gaacataaat 3901 ggcaacatct tgggattatg aacatcatgt tgatctcgaa gacctgttgc tgctcagaca 3961 gcagaaatat agccttaaac aagcactgtc ctgtcctcaa ggaatgatct aaaggaggaa 4021 aaacaataat tacacaatta atattcttaa ttaagacttt aagagccatg gcaatgttca 4081 agcaggacca aatgtggtgc agtgttgagg gcaatctggg aaggttcctt aagaagttat 4141 gttctgagac cattctcagt ggctcaacaa cacttggtca aaaattttaa ttctcccctc 4201 agagaaatgg agtagttact ccactttcaa gttccttata agcttaccat caaccttata 4261 gtacactcta gatgtcctga aatatttcta tcagaacaag gtagtataaa gctggtaggt 4321 atacaaaacg ctagactagt ttctatccct gacccttaat ctgctagtat atccgtagga 4381 agttgcttaa gtgccactag taccaacagc ctctgttaga gtgaacagta gattttaatg 4441 aagggccaat aaagaccact taagactact gactgaatta aaaatccacc cagtggagtt 4501 agtattggtt atagttatat aacaaaatat ccaacttagc caggcgtggt ggtgcacgcc 4561 tttaatcccg gcactcagga ggcagaggca ggcggatttc tgagttcgag gccagcctgg 4621 tctacaaagt gagttccagg acagccaggg ctatacagag aaaccctgtc tcgaaaaacc 4681 aaaaaaaaaa aaaaaaagga aaaaaaatat ggcttgaaaa catgctattg ttgtcaaaag 4741 gagatccaaa caagtatttc tttgaacaaa aattatgtag aaaacagggt gataaagaac 4801 aagggctttt taaaaaaggc cagcattatt tgagatatac aagtgactcc aattcaagcc 4861 taaaggccac tcaatgctca ctaacagtgc taatcagaat caattcctta gcccagtcaa 4921 aaatagtcct gaatatcttc tggataaaca tgtcctccaa catataaact agtatacact 4981 aacaaaacgt ctcaacttca aggtgaaatg cttgactcct agacttgtga cccagcaaag 5041 tgctctatag gtagggttac taggtcagag agtcttgcct gcaaaccaca attatacaat 5101 tccaccaaat gtaagacatg taaaaatgac ttatttagct caggttttga aaattaaaaa 5161 tcaaacccac aaagacctaa ctttcacatt aagtacaaat gtttaacata tataacatgt 5221 tttgccttga tatatgaaat catatgacat cacctgacag gaaacttaag tttatttgaa 5281 caacaatcag cctaaggtag gcctagcaca tgatcaggat agtgcaggga aacccaaaga 5341 agtgcttctg agtataaacc aagagacctc gaaatagcag ctttgttctg tatctcatgg 5401 gctttaacta tcacttgaaa acaatttcac aaatcacaaa accaacattt gtttagtttc 5461 caactctgcc tattcaccgg agaaatccat gtcccaattt gatgggggaa aacttgaatg 5521 aaatattatt tggaatattt taatatccct gtgtatatgc agatggttta aagacaggta 5581 accttaactg tagtttcttg gttgtctgcc ttgttttttg aaatatgctc tcaccaggag 5641 cctgccaagt aactactctt gtgtgctcag caagtcctag ggatccctta agcatgctct 5701 aacaggcctg gctttttttt ttaaaaagta ggttgcggtg ataataattc aagttttcat 5761 cacttgaaac acattttacc aactatcacc caagctccct tagtgtgtcc cctataaaag 5821 aatctgacct gcaagttcca aaagtattac tacattttaa ccttagtatt caggagacaa 5881 aagcaggcag aactttagtc tacatatctt gttgcagatc aggcagagat acatagtgag 5941 accctgcctc aggaaaagct atcattttta tattcacagt aagcaaatta acattaaaag 6001 tcagaaattc ttaatttgac tttggctgtg aagaatttgg attctctgga tttgaaacta 6061 gaaaatctga ttaagcgatg caacagtttc tgtatactaa aactctaaga cccttggttc 6121 taaagatacc acatttaaaa aaaaagttaa gtataagaca aaaagcctta atagctcttt 6181 tataggggag ggactcattt aatattagtc cacctcactc ctcataacta ttttatgagg 6241 tttattatat aacccatttt ttaaaaaaaa aattaaagga caattttagt gtttgaaaga 6301 tttcccaacc ccaccctgga aatcaggctg caaatctcag cagctgccct tagaaggcaa 6361 aaagccatgc catttaagcc atgggaagtt agtagcaaac aagagaccac atggcaggaa 6421 ggaggcttta aagaaagccc acagtgtcaa aagacccaac caacagcaga gacagaaaaa 6481 ataataccca aatctcatca gaaaggtaga cggatttagc cacatccata gtggctcatt 6541 agggaatgct tcaattataa ttttatgaaa tctcagagcc cccctcccca acagggtttc 6601 tgtgtgtatt cctggctatc ctagatagaa cttgatgtgt agaccaggct gggctcaaac 6661 tcagagatct gcctgtctct gcctccagag tgctgggatt aaaggcataa aggcaagcac 6721 caccactggc tggctaaact ctggccctac atttttgaaa tccaaaatta tcattacttg 6781 ctttcatgtt tagtaatggc tgcagactta gctttcagct ttgtatagat gaagcacaaa 6841 agttaagtaa agctagcagt ttaaacaaat aggatactag aagttcaatt tttatttctt 6901 tattgcttag tgtaagagaa aagacattaa agtctgcatt ggtcttctgt atcccacaag 6961 tctgcagtta tggctcctct gtccacagtt acacttcaaa taaaaattag ttccttggta 7021 acatttccag tgcttaaaga gtaatttgat tattggctac catattggaa caaacacaaa 7081 gtatttcatt acttcagtaa attctactgg agacagtatt ctctttaaaa aaaaaaaaaa 7141 gaagaagaag gaggatttat ttatttaatg tgaatacact tgtggtcttc agacacacca 7201 gaagagggca tcagatccca ttacagatgg ttgtgagcca ccatgtggtt gctgggaatt 7261 gaactcagga cttctggaag agcagtctgt gctcttgaac tccgagccac ctctccagcc 7321 cctggatagt attattatag agcacaagca cacacaacac aacaacctaa aaaccaagct 7381 tcacaaatac atttgttgct taatcatttt acttgtgttt ggctacaagt caagcaaaat 7441 tataggtcct gaagaagctt ggcaaaatca catttagacc agcaataacg tgtagaatgc 7501 catgagtcaa gccagtccaa gagaaagcac tgcttaaata gaaaaaatac aagctcacaa 7561 gaccttaggt caggaaagac aacaaacacc tgaactttgc attccaaaag gaaccacctt 7621 ttacagatgt gtacaatgac aaaattttag taagcagtaa tcaataccat gtggctcaat 7681 aatgaaatat accttttaat gtcttaaaat atcagcctgg caatatgtaa gatacatcag 7741 gtaatattgg gggaggagac atccacctgg aaaccattaa tggttaatag ctcagtttat 7801 aaatggagaa aaaggagaga ggcattcatg ggagtggaaa gttaagcttt aggttggatt 7861 ctcaatacat ctattgttgt aaaactccct ggactgagaa taggcccaaa tgtggaacac 7921 cacctgacgg gagaggtgat agacactgaa aattaaggat caaggcaaag gatcaacaaa 7981 aagtgtacta aggagttata aaagaactgc agtgttgagg ccccagctac agcctcgatt 8041 tgtggtgtat gtaactaatc tgtctggttt catgagtcat cagacttcta agatcaggaa 8101 agggaaaatg ccaatgctct gtctaggggt tggataagcc agtataataa atgaaaatgg 8161 ggctaaaatg agtgttctaa aatacctttt gataaggctg cagaaggagc gggagaaatg 8221 gatatgaagt actgggctct ttaaaaatga ttaaaattct gcttacatag tctaactcgc 8281 gacactgtaa tttcatactg tagtaaggat ctcaagcagg agagtataaa actcgggtga 8341 gcatgtcttt aatctacctg gtttgtaccg tacaccactg agtccgccgt cgtcgacaag 8401 ccggcccatg attacgcgaa tttaaatcgg atccatatgg cgcgccggat ctcgacattg 8461 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat 8521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc 8581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca 8641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta 8701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta 8761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat 8821 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc 8881 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc 8941 gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc 9001 gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat 9061 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc 9121 tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct 9181 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg 9241 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga 9301 ggggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg tgcgtgtgtg 9361 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc 9421 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg 9481 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg 9541 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc 9601 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg 9661 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc 9721 ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc 9781 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac 9841 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta 9901 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc 9961 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg 10021 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg 10081 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag atccttaatt 10141 aagccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 10201 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg 10261 ccacctacgg caagctgacc ctgaagctga tctgcaccac cggcaagctg cccgtgccct 10321 ggcccaccct cgtgaccacc ctgggctacg gcctgcagtg cttcgcccgc taccccgacc 10381 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc cagactccag 10441 tctttctaga agatggagta cccatcttct tcaaggacga cggcaactac aagacccgcg 10501 ccgaggtgaa gttcgagggc gacaccctgg tcaaccgcat cgagctcaag ggcatcgact 10561 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg 10621 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca 10681 acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg 10741 acggccccgt gctgctgccc gacaaccact acctcagcta ccagtccgcc ctcagcaaag 10801 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtcaccgcc gccgggatca 10861 ctctcggcat ggacgagctg tacaagtagc cgccacgaac ttctctctgt taaagcaagc 10921 aggagatgtt gaagaaaacc ccgggcctat ggtgggtgag gatagcgtgc tgatcaccga 10981 gaacatgcac atgaaactgt acatggaggg caccgtgaac gaccaccact tcaagtgcac 11041 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgaagatca aggtggtcga 11101 gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcaa 11161 aacctttatc aaccacaccc agggcatccc cgacttcttt aagcagtcct tccctgaggg 11221 cttcacatgg gagaggatca ccacatacga agacgggggc gtgctgaccg ctacccagga 11281 caccagcctc cagaacggct gcctcatcta caacgtcaag atcaacgggg tgaacttccc 11341 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccagca ccgagatgct 11401 gtaccccgct gacagcggcc tgagaggcca tgcccagatg gccctgaagc tcgtgggcgg 11461 gggctacctg cactgctccc tcaagaccac atacagatcc aagaaacccg ctaagaacct 11521 caagatgccc ggcttctact tcgtggaccg gagactggaa agaatcaagg aggccgacaa 11581 agagacctac gtcgagcagc acgagatggc tgtggccaga tactgcgacc tgcctagcaa 11641 actggggcac agctaagcga tcgcacgcgt aaatgattgc agatccacta gttctagagc 11701 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc 11761 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga 11821 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga 11881 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat 11941 ggcttctgag gcggaaagaa ccagctgggg ctcgagatcc actagttcta gcctcgaggc 12001 tagagcggcc gcatatgaat tcatagtacc ggttgtaacg cgtgctagca gagctcgagt 12061 actcagatct aagcttggat gatcctgacg acggagaccg cggtggttga ccagacaaac 12121 cggtttgttc tagaaagact ggagttgcag atcacgaggg aagaggggga agggattctc 12181 ccaggcccag ggcggtcctc agaagccagg aggcagcaga gaactcccag aaaggtattg 12241 caacactccc ctcccccctc cggagaaggg tgcggccttc tccccgccta ctccactgca 12301 gctcccttac tgataacaac tcagagcgac tttgggagag caagtgcttc ctgcctccaa 12361 aacagcccaa ctgagccctc gctccttccc tccactcccc ggagtgcgcg atggaggtct 12421 ggctcagcac gcccctcttg aggcaactca agtcggaaac gtgcttgcac ccgccccgca 12481 gccgctcagc cctactgccc gtccccgccc ccagcgcgcg cttcctgcca cgttgcgcag 12541 gggcgcgcgg ccagactctg cggcgcgggg ccgaggggag ggccggaacc tgggagcgcc 12601 tcctcgccgc ccccgctggc cggcggatgg actcaacttg cacgaacacg agccaatggc 12661 aagggccagt tttctgggcc ccgagagcca atcagacgac gaggcccggc cggcggcggg 12721 taaaacgact cccccagagg aaggggaggg tgggcggccg ctcgcgcgcg agctactttc 12781 gctgaccctc cctcccctcc cccgccccgc cagaggccga ccgcgcccgc acgtccagct 12841 cgcctcaccc cacctacctc ccgccccacc cagtgggcag agcgaggctg ccggcggctg 12901 cgcactccgg ctgccgttaa ctgacaggcg ccttacgcca accaaaacac gccatttgtg 12961 ttttcacaca cggcgggagg aaaagaagcc aatcagcgac gagacgtcgg ccggaagcgc 13021 tcctccgctg cccccccccc cccgagccat ggccgcgtcc ggtggagact tttccgctcc 13081 cttctccctc cccctccggt tgctgcaggg cggaccgcat tcctgcccac cacccgcttg 13141 ccccttccag cgtcacgact cgtacccggc tgtctcacag aacggctcca ccacgctcgg 13201 agggcctgcc gcggccgccc atccccgcaa acgcaccaag ccgccctccc gccagagtcc 13261 cgatccccta cctagccgag gctctctgag gagccggagc cccaaagcac agcctcttct 13321 ttaggcggcc ccggcagcct ccgctgattg gcggcgcacc ggccaataag tgtggggcgg 13381 tgggcggaga ggccaatggc gcggcgggag ggggcgtgtc ccgcgtgccc ctggcgccgg 13441 cactgggaat ccccgtgcgg tcagtggcgt ttccgctcgg gcagcgggct gagtgagctg 13501 ccgccgctgc cgccgctgcc gccgccgggg gaggggcggc cgccgcccgc ctgcgctcag 13561 agactcacgc agccctagtc ccgccagtcc gccaacacag tagtgccggc cccctccgcc 13621 cctggccctc cccctccccg cctcgggctc gctccgcctt ttctgccccc cacccccacc 13681 tcacgggtac gggccattcc cggccaggaa acgccgtggc gccgcgttgg gcctaactcg 13741 agtctcgctg cctcccggga gtgccgtgcg ccgcagcccg ggcccaggcc ccggcagcgc 13801 ctgggtcaag gtaagggtcc aacagaaaag agacaccgaa ccacacgacc gggccccggg 13861 ggaaagaagg gtcacctcag tctccctgcg ttccctctcc tcggggtcgt gggcctgggc 13921 ctccccagcc tggtagcagg aagatgcttg cccttttcgt cttctcagct acctttacac 13981 accattgcac cgctcttgcc cagagagaaa ggctctcctt catctagtcg accccactac 14041 ctttttaatg tcttccctgg gtcaggactc ttcccctccc cctactctgg tctccccttt 14101 ttgcctgggt attgcctact ccacgtttat acccttttca ggagaggcct cccaaccctg 14161 ctctcaaaat acacatactt ttttttctgt ccctgagccc cccacctccc ctgttcttgc 14221 ggccttgtga caactctggt cgctcgtggg ggcccagtcc tcccctccat aatcttcctg 14281 aacgcctctc ctctggtttt ccagttccta tctcagatgg ctgctgcttt tcccacacca 14341 aagacattac cttcgccacc cccacctcac attcttggac tccctgtggc gtatgcccca 14401 gtatccttgc gatatccctg ttgaatttgt a //