LOCUS Exported 13921 bp DNA linear SYN 14-SEP-2022 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13921) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 14, 2022 from SnapGene 6.0.5 https://www.snapgene.com FEATURES Location/Qualifiers source 1..13921 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 308..318 /label=2250 /note="color: black; sequence: GAGGGGCGTGCTGAGCCAGACCTCCATCGCGCACTCCGGGGAGTGGAGGGAAGGAGCGA GGGCTCAGTTGGGCTGGGTTTGTCTGGTCAACCACCgcggActcAGTGGTGTACGGTAC AAACCTTTTGGAGGCAGGAAGCACTTGCTCTCCCAAAGTCGCTCTGAGTTGTTATCAGT AAGGGAGCTGCAGTGGAGTAGGC; added: 2020-03-17" primer_bind 434..457 /label=1211 /note="color: black; sequence: CAAGAGCGGTGCAATGGTGTGTAA" primer_bind 607..627 /label=1911 /note="color: black; sequence: CTGTTGGACCCTTACCTTGAC" misc_feature join(1001..2304,5563..13921) /label=Gt(ROSA)26Sor - MGI:104735 /note="Gt(ROSA)26Sor - MGI:104735" /note="This feature has 2 segments: 1: 1001 .. 2304 / #ff6666 2: 5563 .. 13921 / #ff6666" misc_feature 1090..1266 /note="color: #333333; direction: RIGHT" primer_bind 1111..1131 /label=1209 /note="color: black; sequence: AAGAAGAGGCTGTGCTTTGGG" primer_bind 1241..1258 /label=2846 /note="color: black; sequence: GTGGAGCCGTTCTGTGAG; added: 2020-03-17" primer_bind complement(1361..1377) /label=2051 /note="color: black; sequence: GCGTCCGGTGGAGACTT" primer_bind 1431..1449 /label=2708 /note="color: black; sequence: AAATTCGAATTCCGTCGCTGATTGGCTTCTt; added: 2020-03-17" primer_bind 1431..1449 /label=2705 /note="color: black; sequence: CGTCGCTGATTGGCTTCTt; added: 2020-03-17" primer_bind complement(1645..1663) /label=2047 /note="color: black; sequence: CGAGCTACTTTCGCTGACC" primer_bind 1773..1797 /label=2709 /note="color: black; sequence: AAATTCGAATTCCATTGGCTCGTGTTCGTGCAAGTT; added: 2020-03-17" primer_bind 1774..1797 /label=1069 /note="color: black; sequence: CATTGGCTCGTGTTCGTGCAAGTT" primer_bind 1965..1985 /label=2712 /note="color: black; sequence: AAATTCGAATTCAAGCACGTTTCCGACTTGAg; added: 2020-03-17" primer_bind 1966..1985 /label=2711 /note="color: black; sequence: AAGCACGTTTCCGACTTGAg; added: 2020-03-17" primer_bind 1966..1984 /label=1813 /note="color: black; sequence: AAGCACGTTTCCGACTTGA" primer_bind 1995..2146 /label=2250 /note="color: black; sequence: GAGGGGCGTGCTGAGCCAGACCTCCATCGCGCACTCCGGGGAGTGGAGGGAAGGAGCGA GGGCTCAGTTGGGCTGGGTTTGTCTGGTCAACCACCgcggActcAGTGGTGTACGGTAC AAACCTTTTGGAGGCAGGAAGCACTTGCTCTCCCAAAGTCGCTCTGAGTTGTTATCAGT AAGGGAGCTGCAGTGGAGTAGGC; added: 2020-03-17" primer_bind 2093..2116 /label=1207 /note="color: black; sequence: TCTCCCAAAGTCGCTCTGAGTTGT" primer_bind complement(2115..2136) /label=2819 /note="color: black; sequence: CTGCAGCTCCCTTACTGATAAC; added: 2020-03-17" primer_bind 2181..2201 /label=1141 /note="color: black; sequence: GGGGAGTGTTGCAATACCTTT" primer_bind 2199..2221 /label=1076 /note="color: black; sequence: TTTCTGGGAGTTCTCTGCTGCCT" primer_bind 2212..2236 /label=1310 /note="color: black; sequence: TCTGCTGCCTCCTGGCTTCTGAGGA" primer_bind 2279..2300 /label=2719 /note="color: black; sequence: GATCTGCAACTCCAGTCTTTCT; added: 2020-03-17" misc_feature 2286..2353 /label=Rosa-attP-GT-aatB (B.Low) /note="color: #a6acb3" misc_feature 2298..2303 /label=XbaI /note="color: #a6acb3" misc_feature 2305..2310 /label=INDEL /note="GET 314 INS" /note="color: #ffff00" primer_bind complement(2311..2338) /label=1928 /note="color: black; sequence: GGTTTGTACCGTACACCACTGAGACCGCGGTGGTTGACCAGACAAACC" primer_bind 2311..2338 /label=1927 /note="color: black; sequence: GGTTTGTCTGGTCAACCACCGCGGTCTCAGTGGTGTACGGTACAAACC" misc_feature 2311..2335 /label=Bxb1 AttP Site /note="Bxb1 AttP Site" /note="color: #ff0000" primer_bind 2311..2335 /label=2161 /note="color: black; sequence: GGTTTGTCTGGTCAACCACCgcggt" misc_feature 2336..2353 /label=(GT) Bxb1 attB (B') /note="color: #00ff00; direction: RIGHT Cleavage site after base 2335" promoter 2419..3596 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" /note="color: #ffffff; direction: RIGHT" intron 2649..3587 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" /note="color: #------" promoter 3613..3631 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" /note="color: #ffffff; direction: RIGHT" protein_bind 3662..3686 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" /note="color: #31849b; direction: RIGHT" misc_feature 3707..3811 /label=EGFP /note="color: #00ff00" primer_bind complement(3734..3744) /label=2161 /note="color: black; sequence: GGTTTGTCTGGTCAACCACCgcggt" misc_feature 4434 /label=Putative splicing enhancer /note="color: #ff9900" misc_feature 4436..4455 /label=gRNA1 /note="color: #800000; direction: RIGHT" misc_feature 4442..4461 /label=gRNA2 /note="color: #800000; direction: RIGHT" misc_feature 4465 /label=IVS2-654 Target Mutation /note="color: #ff0000" misc_feature 4502..4521 /label=gRNA3 /note="color: #800000; direction: RIGHT" misc_feature 4662..5276 /label=EGFP /note="color: #00ff00" polyA_signal 5277..5484 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" /note="color: #a6acb3" primer_bind 5515..5592 /label=2580 /note="color: black; sequence: GCAAAACTACAGGTTATTATTGCTTGTGATCCGCCTCGGAGTATTTTCCATCGGGCCGG CTTGTCGACGACGGCGGTCTCCGTCGTCAGGATCATCCGGAGGTAGATTAAAGACATGC TCACCCGAGTT; added: 2020-03-17" misc_feature 5520..5537 /label=Bxb1 attB B (GA) /note="color: #ff6600; direction: RIGHT" primer_bind 5534..5551 /label=2493 /note="color: black; sequence: gGCGGaCTCAGTGGTGTA; added: 2020-03-17" primer_bind 5535..5592 /label=2255 /note="color: black; sequence: GCAAAACTACAGGTTATTATTGCTTGTGATCCGCCTCGGAGTATTTTCCATCGGGTTTG TCTGGTCAACCACCGCGGACTCAGTGGTGTACGGTACAAACCAGGTAGATTAAAGACAT GCTCACCCGAGTT; added: 2020-03-17" primer_bind 5535..5562 /label=1927 /note="color: black; sequence: GGTTTGTCTGGTCAACCACCGCGGTCTCAGTGGTGTACGGTACAAACC" primer_bind 5535..5551 /label=1956 /note="color: black; sequence: CGCGGTCTCAGTGGTGTA" misc_feature 5538..5562 /label=Bxb1 attP Site (PP') GA /note="color: #ff9900" primer_bind complement(5540..5562) /label=2162 /note="color: black; sequence: GGTTTGTACCGTACACCACTgag" primer_bind complement(5540..5562) /label=1928 /note="color: black; sequence: GGTTTGTACCGTACACCACTGAGACCGCGGTGGTTGACCAGACAAACC" primer_bind complement(5576..5595) /label=1140 /note="color: black; sequence: TAAAACTCGGGTGAGCATGT" primer_bind complement(5631..5652) /label=2718 /note="color: black; sequence: GTCTAACTCGCGACACTGTAAT; added: 2020-03-17" primer_bind complement(5696..5716) /label=2488 /note="color: black; sequence: GGAGCGGGAGAAATGGATATG; added: 2020-03-17" primer_bind complement(5711..5734) /label=1068 /note="color: black; sequence: TTTGATAAGGCTGCAGAAGGAGCG" primer_bind 5780..5941 /label=2682 /note="color: black; sequence: CTGGCTTATCCAACCCCTAGACAGAGCATTGGCATTTTCCCTTTCCTGATCTTAGAAGT CTGATGACTCATGAAACCAGACGGCTTGTCGACGACGGCGGTCTCCGTCGTCAGGATCA TAGATTAGTTACATACACCACAAATCGAGGCTGTAGCTGGGGCCTCAACACTGCAGTTC TTTTATAACTCCTTAGTACACTT; added: 2020-03-17" primer_bind 5780..5941 /label=2685 /note="color: black; sequence: CTGGCTTATCCAACCCCTAGACAGAGCATTGGCATTTTCCCTTTCCTGATCTTAGAAGT CTGATGACTCATGAAACCAGACGGCTTGTCGACGACGGCGGTCTCCGTCGTCAGGATCA TAGATTAGTTACATACACCACAAATCGAGGCTGTAGCTGGGGCCTCAACACTGCAGTTC TTTTATAACTCCTTAGTACACTT; added: 2020-03-17" primer_bind complement(5795..5815) /label=1814 /note="color: black; sequence: AATGCCAATGCTCTGTCTAGG" primer_bind complement(6083..6106) /label=1103 /note="color: black; sequence: AGAGAGGCATTCATGGGAGTGGAA" primer_bind 6083..6106 /label=1096 /note="color: black; sequence: TTCCACTCCCATGAATGCCTCTCT" primer_bind complement(6395..6415) /label=2710 /note="color: black; sequence: TCATCCGGATCCTCAAGCCAGTCCAAGAGAAAG; added: 2020-03-17" primer_bind complement(6395..6415) /label=2680 /note="color: black; sequence: TCAAGCCAGTCCAAGAGAAAG; added: 2020-03-17" primer_bind complement(6395..6415) /label=2707 /note="color: black; sequence: TCAAGCCAGTCCAAGAGAAAG; added: 2020-03-17" primer_bind 6711..6734 /label=1097 /note="color: black; sequence: ATGCCCTCTTCTGGTGTGTCTGAA" primer_bind complement(6947..6970) /label=1104 /note="color: black; sequence: TCCCACAAGTCTGCAGTTATGGCT" primer_bind complement(6968..6989) /label=2847 /note="color: black; sequence: TCTGCATTGGTCTTCTGTATCC; added: 2020-03-17" primer_bind complement(7218..7233) /label=1324 /note="color: black; sequence: TCCCAAGTGCTGGGATTAAAG; added: 2020-03-17" primer_bind complement(7218..7233) /label=1448 /note="color: black; sequence: TCCCAAGTGCTGGGATTAAAG; added: 2020-03-17" primer_bind 7698..7719 /label=1328 /note="color: black; sequence: TGAGGAGTGAGGTGGACTAATA" primer_bind 8080..8099 /label=1326 /note="color: black; sequence: TTTGGAACTTGCAGGTCAGA" primer_bind 8121..8144 /label=1099 /note="color: black; sequence: AAGGGAGCTTGGGTGATAGTTGGT" primer_bind complement(8426..8449) /label=1106 /note="color: black; sequence: TTCACCGGAGAAATCCATGTCCCA" primer_bind complement(8441..8460) /label=1327 /note="color: black; sequence: AACTCTGCCTATTCACCGGA" primer_bind complement(8589..8609) /label=1329 /note="color: black; sequence: ATCAGGATAGTGCAGGGAAAC" primer_bind 8834..8857 /label=1100 /note="color: black; sequence: TGGTTTGCAGGCAAGACTCTCTGA" primer_bind complement(8884..8907) /label=1107 /note="color: black; sequence: ACTCCTAGACTTGTGACCCAGCAA" primer_bind complement(9275..9298) /label=1278 /note="color: black; sequence: ACAAAGTGAGTTCCAGGACAGCCA" primer_bind 9275..9298 /label=1101 /note="color: black; sequence: TGGCTGTCCTGGAACTCACTTTGT" primer_bind 9347..9362 /label=1324 /note="color: black; sequence: TCCCAAGTGCTGGGATTAAAG; added: 2020-03-17" primer_bind 9347..9362 /label=1448 /note="color: black; sequence: TCCCAAGTGCTGGGATTAAAG; added: 2020-03-17" primer_bind complement(9760..9781) /label=1912 /note="color: black; sequence: GTTCTGAGACCATTCTCAGTGG" exon 9914..10951 /label=Exon 2 variant 1 lncRNA /note="color: #333333; direction: RIGHT" polyA_signal 10926..10930 /label=PolyA Signal /note="color: #a6acb3" ORIGIN 1 tacaaattca acagggatat cgcaaggata ctggggcata cgccacaggg agtccaagaa 61 tgtgaggtgg gggtggcgaa ggtaatgtct ttggtgtggg aaaagcagca gccatctgag 121 ataggaactg gaaaaccaga ggagaggcgt tcaggaagat tatggagggg aggactgggc 181 ccccacgagc gaccagagtt gtcacaaggc cgcaagaaca ggggaggtgg ggggctcagg 241 gacagaaaaa aaagtatgtg tattttgaga gcagggttgg gaggcctctc ctgaaaaggg 301 tataaacgtg gagtaggcaa tacccaggca aaaaggggag accagagtag ggggagggga 361 agagtcctga cccagggaag acattaaaaa ggtagtgggg tcgactagat gaaggagagc 421 ctttctctct gggcaagagc ggtgcaatgg tgtgtaaagg tagctgagaa gacgaaaagg 481 gcaagcatct tcctgctacc aggctgggga ggcccaggcc cacgaccccg aggagaggga 541 acgcagggag actgaggtga cccttctttc ccccggggcc cggtcgtgtg gttcggtgtc 601 tcttttctgt tggaccctta ccttgaccca ggcgctgccg gggcctgggc ccgggctgcg 661 gcgcacggca ctcccgggag gcagcgagac tcgagttagg cccaacgcgg cgccacggcg 721 tttcctggcc gggaatggcc cgtacccgtg aggtgggggt ggggggcaga aaaggcggag 781 cgagcccgag gcggggaggg ggagggccag gggcggaggg ggccggcact actgtgttgg 841 cggactggcg ggactagggc tgcgtgagtc tctgagcgca ggcgggcggc ggccgcccct 901 cccccggcgg cggcagcggc ggcagcggcg gcagctcact cagcccgctg cccgagcgga 961 aacgccactg accgcacggg gattcccagt gccggcgcca ggggcacgcg ggacacgccc 1021 cctcccgccg cgccattggc ctctccgccc accgccccac acttattggc cggtgcgccg 1081 ccaatcagcg gaggctgccg gggccgccta aagaagaggc tgtgctttgg ggctccggct 1141 cctcagagag cctcggctag gtaggggatc gggactctgg cgggagggcg gcttggtgcg 1201 tttgcgggga tgggcggccg cggcaggccc tccgagcgtg gtggagccgt tctgtgagac 1261 agccgggtac gagtcgtgac gctggaaggg gcaagcgggt ggtgggcagg aatgcggtcc 1321 gccctgcagc aaccggaggg ggagggagaa gggagcggaa aagtctccac cggacgcggc 1381 catggctcgg gggggggggg gcagcggagg agcgcttccg gccgacgtct cgtcgctgat 1441 tggcttcttt tcctcccgcc gtgtgtgaaa acacaaatgg cgtgttttgg ttggcgtaag 1501 gcgcctgtca gttaacggca gccggagtgc gcagccgccg gcagcctcgc tctgcccact 1561 gggtggggcg ggaggtaggt ggggtgaggc gagctggacg tgcgggcgcg gtcggcctct 1621 ggcggggcgg gggaggggag ggagggtcag cgaaagtagc tcgcgcgcga gcggccgccc 1681 accctcccct tcctctgggg gagtcgtttt acccgccgcc ggccgggcct cgtcgtctga 1741 ttggctctcg gggcccagaa aactggccct tgccattggc tcgtgttcgt gcaagttgag 1801 tccatccgcc ggccagcggg ggcggcgagg aggcgctccc aggttccggc cctcccctcg 1861 gccccgcgcc gcagagtctg gccgcgcgcc cctgcgcaac gtggcaggaa gcgcgcgctg 1921 ggggcgggga cgggcagtag ggctgagcgg ctgcggggcg ggtgcaagca cgtttccgac 1981 ttgagttgcc tcaagagggg cgtgctgagc cagacctcca tcgcgcactc cggggagtgg 2041 agggaaggag cgagggctca gttgggctgt tttggaggca ggaagcactt gctctcccaa 2101 agtcgctctg agttgttatc agtaagggag ctgcagtgga gtaggcgggg agaaggccgc 2161 acccttctcc ggagggggga ggggagtgtt gcaatacctt tctgggagtt ctctgctgcc 2221 tcctggcttc tgaggaccgc cctgggcctg ggagaatccc ttccccctct tccctcgtga 2281 tctgcaactc cagtctttct agaacaaacc ggtttgtctg gtcaaccacc gcggtctccg 2341 tcgtcaggat catccaagct tagatctgag tactcgagct ctgctagcac gcgttacaac 2401 cggtactatg aattcgatgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag 2461 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg 2521 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag 2581 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca 2641 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc 2701 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc 2761 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg 2821 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc 2881 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg 2941 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta 3001 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg 3061 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc 3121 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc 3181 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag 3241 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa 3301 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc 3361 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg 3421 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag 3481 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca 3541 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa 3601 ttagcttggt actaatacga ctcactatag ggagacccaa gctggctagg taagcttgat 3661 cacaagtttg tacaaaaaag caggctccgc ggccgccccc ttcaccatgg tgagcaaggg 3721 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 3781 ccacaagttc agcgtgtccg gcgagggcga ggtgagtcta tgggaccctt gatgttttct 3841 ttccccttct tttctatggt taagttcatg tcataggaag gggagaagta acagggtaca 3901 gtttagaatg ggaaacagac gaatgattgc atcagtgtgg aagtctcagg atcgttttag 3961 tttcttttat ttgctgttca taacaattgt tttcttttgt ttaattcttg ctttcttttt 4021 ttttcttctc cgcaattttt actattatac ttaatgcctt aacattgtgt ataacaaaag 4081 gaaatatctc tgagatacat taagtaactt aaaaaaaaac tttacacagt ctgcctagta 4141 cattactatt tggaatatat gtgtgcttat ttgcatattc ataatctccc tactttattt 4201 tcttttattt ttaattgata cataatcatt atacatattt atgggttaaa gtgtaatgtt 4261 ttaatatgtg tacacatatt gaccaaatca gggtaatttt gcatttgtaa ttttaaaaaa 4321 tgctttcttc ttttaatata cttttttgtt tatcttattt ctaatacttt ccctaatctc 4381 tttctttcag ggcaataatg atacaatgta tcatgcctct ttgcaccatt ctaaagaata 4441 acagtgataa tttctgggtt aaggtaatag caatatttct gcatataaat atttctgcat 4501 ataaattgta actgatgtaa gaggtttcat attgctaata gcagctacaa tccagctacc 4561 attctgcttt tattttatgg ttgggataag gctggattat tctgagtcca agctaggccc 4621 ttttgctaat catgttcata cctcttatct tcctcccaca gggcgatgcc acctacggca 4681 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg 4741 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc 4801 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca 4861 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga 4921 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc 4981 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca 5041 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc 5101 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc 5161 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc 5221 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaactgt 5281 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga 5341 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag 5401 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga 5461 agacaatagc aggcatgctg gggaatcgga tccgatttaa attcgcgtaa tcatgggccg 5521 gcttgtcgac gacggcggac tcagtggtgt acggtacaaa ccaggtagat taaagacatg 5581 ctcacccgag ttttatactc tcctgcttga gatccttact acagtatgaa attacagtgt 5641 cgcgagttag actatgtaag cagaatttta atcattttta aagagcccag tacttcatat 5701 ccatttctcc cgctccttct gcagccttat caaaaggtat tttagaacac tcattttagc 5761 cccattttca tttattatac tggcttatcc aacccctaga cagagcattg gcattttccc 5821 tttcctgatc ttagaagtct gatgactcat gaaaccagac agattagtta catacaccac 5881 aaatcgaggc tgtagctggg gcctcaacac tgcagttctt ttataactcc ttagtacact 5941 ttttgttgat cctttgcctt gatccttaat tttcagtgtc tatcacctct cccgtcaggt 6001 ggtgttccac atttgggcct attctcagtc cagggagttt tacaacaata gatgtattga 6061 gaatccaacc taaagcttaa ctttccactc ccatgaatgc ctctctcctt tttctccatt 6121 tataaactga gctattaacc attaatggtt tccaggtgga tgtctcctcc cccaatatta 6181 cctgatgtat cttacatatt gccaggctga tattttaaga cattaaaagg tatatttcat 6241 tattgagcca catggtattg attactgctt actaaaattt tgtcattgta cacatctgta 6301 aaaggtggtt ccttttggaa tgcaaagttc aggtgtttgt tgtctttcct gacctaaggt 6361 cttgtgagct tgtatttttt ctatttaagc agtgctttct cttggactgg cttgactcat 6421 ggcattctac acgttattgc tggtctaaat gtgattttgc caagcttctt caggacctat 6481 aattttgctt gacttgtagc caaacacaag taaaatgatt aagcaacaaa tgtatttgtg 6541 aagcttggtt tttaggttgt tgtgttgtgt gtgcttgtgc tctataataa tactatccag 6601 gggctggaga ggtggctcgg agttcaagag cacagactgc tcttccagaa gtcctgagtt 6661 caattcccag caaccacatg gtggctcaca accatctgta atgggatctg atgccctctt 6721 ctggtgtgtc tgaagaccac aagtgtattc acattaaata aataaatcct ccttcttctt 6781 cttttttttt tttttaaaga gaatactgtc tccagtagaa tttactgaag taatgaaata 6841 ctttgtgttt gttccaatat ggtagccaat aatcaaatta ctctttaagc actggaaatg 6901 ttaccaagga actaattttt atttgaagtg taactgtgga cagaggagcc ataactgcag 6961 acttgtggga tacagaagac caatgcagac tttaatgtct tttctcttac actaagcaat 7021 aaagaaataa aaattgaact tctagtatcc tatttgttta aactgctagc tttacttaac 7081 ttttgtgctt catctataca aagctgaaag ctaagtctgc agccattact aaacatgaaa 7141 gcaagtaatg ataattttgg atttcaaaaa tgtagggcca gagtttagcc agccagtggt 7201 ggtgcttgcc tttatgcctt taatcccagc actctggagg cagagacagg cagatctctg 7261 agtttgagcc cagcctggtc tacacatcaa gttctatcta ggatagccag gaatacacac 7321 agaaaccctg ttggggaggg gggctctgag atttcataaa attataattg aagcattccc 7381 taatgagcca ctatggatgt ggctaaatcc gtctaccttt ctgatgagat ttgggtatta 7441 ttttttctgt ctctgctgtt ggttgggtct tttgacactg tgggctttct ttaaagcctc 7501 cttcctgcca tgtggtctct tgtttgctac taacttccca tggcttaaat ggcatggctt 7561 tttgccttct aagggcagct gctgagattt gcagcctgat ttccagggtg gggttgggaa 7621 atctttcaaa cactaaaatt gtcctttaat ttttttttta aaaaatgggt tatataataa 7681 acctcataaa atagttatga ggagtgaggt ggactaatat taaatgagtc cctcccctat 7741 aaaagagcta ttaaggcttt ttgtcttata cttaactttt tttttaaatg tggtatcttt 7801 agaaccaagg gtcttagagt tttagtatac agaaactgtt gcatcgctta atcagatttt 7861 ctagtttcaa atccagagaa tccaaattct tcacagccaa agtcaaatta agaatttctg 7921 acttttaatg ttaatttgct tactgtgaat ataaaaatga tagcttttcc tgaggcaggg 7981 tctcactatg tatctctgcc tgatctgcaa caagatatgt agactaaagt tctgcctgct 8041 tttgtctcct gaatactaag gttaaaatgt agtaatactt ttggaacttg caggtcagat 8101 tcttttatag gggacacact aagggagctt gggtgatagt tggtaaaatg tgtttcaagt 8161 gatgaaaact tgaattatta tcaccgcaac ctacttttta aaaaaaaaag ccaggcctgt 8221 tagagcatgc ttaagggatc cctaggactt gctgagcaca caagagtagt tacttggcag 8281 gctcctggtg agagcatatt tcaaaaaaca aggcagacaa ccaagaaact acagttaagg 8341 ttacctgtct ttaaaccatc tgcatataca cagggatatt aaaatattcc aaataatatt 8401 tcattcaagt tttcccccat caaattggga catggatttc tccggtgaat aggcagagtt 8461 ggaaactaaa caaatgttgg ttttgtgatt tgtgaaattg ttttcaagtg atagttaaag 8521 cccatgagat acagaacaaa gctgctattt cgaggtctct tggtttatac tcagaagcac 8581 ttctttgggt ttccctgcac tatcctgatc atgtgctagg cctaccttag gctgattgtt 8641 gttcaaataa acttaagttt cctgtcaggt gatgtcatat gatttcatat atcaaggcaa 8701 aacatgttat atatgttaaa catttgtact taatgtgaaa gttaggtctt tgtgggtttg 8761 atttttaatt ttcaaaacct gagctaaata agtcattttt acatgtctta catttggtgg 8821 aattgtataa ttgtggtttg caggcaagac tctctgacct agtaacccta cctatagagc 8881 actttgctgg gtcacaagtc taggagtcaa gcatttcacc ttgaagttga gacgttttgt 8941 tagtgtatac tagtttatat gttggaggac atgtttatcc agaagatatt caggactatt 9001 tttgactggg ctaaggaatt gattctgatt agcactgtta gtgagcattg agtggccttt 9061 aggcttgaat tggagtcact tgtatatctc aaataatgct ggcctttttt aaaaagccct 9121 tgttctttat caccctgttt tctacataat ttttgttcaa agaaatactt gtttggatct 9181 ccttttgaca acaatagcat gttttcaagc catatttttt ttcctttttt tttttttttt 9241 tggtttttcg agacagggtt tctctgtata gccctggctg tcctggaact cactttgtag 9301 accaggctgg cctcgaactc agaaatccgc ctgcctctgc ctcctgagtg ccgggattaa 9361 aggcgtgcac caccacgcct ggctaagttg gatattttgt tatataacta taaccaatac 9421 taactccact gggtggattt ttaattcagt cagtagtctt aagtggtctt tattggccct 9481 tcattaaaat ctactgttca ctctaacaga ggctgttggt actagtggca cttaagcaac 9541 ttcctacgga tatactagca gattaagggt cagggataga aactagtcta gcgttttgta 9601 tacctaccag ctttatacta ccttgttctg atagaaatat ttcaggacat ctagagtgta 9661 ctataaggtt gatggtaagc ttataaggaa cttgaaagtg gagtaactac tccatttctc 9721 tgaggggaga attaaaattt ttgaccaagt gttgttgagc cactgagaat ggtctcagaa 9781 cataacttct taaggaacct tcccagattg ccctcaacac tgcaccacat ttggtcctgc 9841 ttgaacattg ccatggctct taaagtctta attaagaata ttaattgtgt aattattgtt 9901 tttcctcctt tagatcattc cttgaggaca ggacagtgct tgtttaaggc tatatttctg 9961 ctgtctgagc agcaacaggt cttcgagatc aacatgatgt tcataatccc aagatgttgc 10021 catttatgtt ctcagaagca agcagaggca tgatggtcag tgacagtaat gtcactgtgt 10081 taaatgttgc tatgcagttt ggatttttct aatgtagtgt aggtagaaca tatgtgttct 10141 gtatgaatta aactcttaag ttacaccttg tataatccat gcaatgtgtt atgcaattac 10201 cattttaagt attgtagctt tctttgtatg tgaggataaa ggtgtttgtc ataaaatgtt 10261 ttgaacattt ccccaaagtt ccaaattata aaaccacaac gttagaactt atttatgaac 10321 aatggttgta gtttcatgct tttaaaatgc ttaattattc aattaacacc gtttgtgtta 10381 taatatatat aaaactgaca tgtagaagtg tttgtccaga acatttctta aatgtatact 10441 gtctttagag agtttaatat agcatgtctt ttgcaacata ctaacttttg tgttggtgcg 10501 agcaatattg tgtagtcatt ttgaaaggag tcatttcaat gagtgtcaga ttgttttgaa 10561 tgttattgaa cattttaaat gcagacttgt tcgtgtttta gaaagcaaaa ctgtcagaag 10621 ctttgaacta gaaattaaaa agctgaagta tttcagaagg gaaataagct acttgctgta 10681 ttagttgaag gaaagtgtaa tagcttagaa aatttaaaac catatagttg tcattgctga 10741 atatctggca gatgaaaaga aatactcagt ggttcttttg agcaatataa cagcttgtta 10801 tattaaaaat tttccccaca gatataaact ctaatctata actcataaat gttacaaatg 10861 gatgaagctt acaaatgtgg cttgacttgt cactgtgctt gttttagtta tgtgaaagtt 10921 tggcaataaa cctatgtcct aaatagtcaa actgtggaat gactttttaa tctattggtt 10981 tgtctagaac agttatgttg ccatttgccc taatggtgaa agaaaaagtg gggagtgcct 11041 tggcactgtt catttgtggt gtgaaccaaa gaggggggca tgcacttaca cttcaaacat 11101 ccttttgaaa gactgacaag tttgggtctt cacagttgga attgggcatc ccttttgtca 11161 gggagggagg gagggaggga ggctggcttg ttatgctgac aagtgtgatt aaattcaaac 11221 tttgaggtaa gttggaggaa cttgtacatt gttaggagtg tgacaatttg gactcttaat 11281 gatttggtca tacaaaatga acctagacca acttctggaa gatgtatata ataactccat 11341 gttacattga tttcacctga ctaatactta tcccttatca attaaataca gaagatgcca 11401 gccatctggg ccttttaacc cagaaattta gtttcaaact cctaggttag tgttctcact 11461 gagctacatc ctgatctagt cctgaaaata ggaccaccat cacccccaaa aaaatctcaa 11521 ataagattta tgctagtgtt tcaaaatttt aggaataggt aagattagaa agttttaaat 11581 tttgagaaat ggcttctcta gaaagatgta catagtgaac actgaatggc tcctaaagag 11641 cctagaaaac tggtactgag cacacaggac tgagaggtct ttcttgaaaa gcatgtattg 11701 ctttacgtgg gtcacagaag gcaggcagga agaacttggg ctgaaactgg tgtcttaagt 11761 ggctaacatc ttcacaactg atgagcaaga actttatcct gatgcaaaaa ccatccaaac 11821 aaactaagtg aaaggtggca atggatccca ggctgctcta gaggaggact tgacttctca 11881 tcccatcacc cacaccagat agctcataga ctgccaatta acaccagctt ctagcctcca 11941 caggcacctg cactggtaca cataatttca cacaaacaca gtaagaagcc ttccacctgg 12001 catggtattg cttatcttta gttcccaaca cttgggaggc agaggccagc cagggctatg 12061 tgacaaaaac cttgtctaga ggagaaactt catagcttat ttcctattca cgtaaccagg 12121 ttagcaaaat ttaccagcca gagatgaagc taacagtgtc cactatattt gtagtgtttt 12181 aagtcaattt tttaaatata cttaatagaa ttaaagctat ggtgaaccaa gtacaaacct 12241 ggtgtattaa cttgagaact tagcataaaa agtagttcat ttgttcagta aatattaaat 12301 gcttactggc aaagattatg tcaggaactt ggtaaatggt gatgaaacaa tcatagttgt 12361 acatcttggt tctgtgatca ccttggtttg aggtaaaagt ggttcctttg atcaaggatg 12421 gaattttaag tttatattca atcaataatg tattattttg tgattgcaaa attgcctatc 12481 tagggtataa aacctttaaa aatttcataa taccagttca ttctccagtt actaattcca 12541 aaaagccact gactatggtg ccaatgtgga ttctgttctc aaaggaagga ttgtctgtgc 12601 cctttattct aatagaaaca tcacactgaa aatctaagct gaaagaagcc agactttcct 12661 aaataaataa ctttccataa agctcaaaca aggattactt ttaggaggca ctgttaagga 12721 actgataagt aatgaggtta cttatataat gatagtccca caagactatc tgaggaaaaa 12781 tcagtacaac tcgaaaacag aacaaccagc taggcaggaa taacagggct cccaagtcag 12841 gaggtctatc caacaccctt ttctgttgag ggccccagac ctacatattg tatacaaaca 12901 gggaggtggg tgattttaac tctcctgagg taccttggta aatctttgtc ctgagtaagc 12961 agtacagtgt acagtttaca ttttcattta aagatacatt agctccctct accccctaag 13021 actgacaggc actttggggg tggggagggc tttggaaaat aacgcttcca tacactaaaa 13081 gagaaatttc tttaattagg cttgttggtt ccatacatct actggtgttt ctactactta 13141 gtaatattat aatagtcaca caagcatctt tgctctgttt aggttgtata tttattttaa 13201 ggcagatgat aaaactgtag atcttaaggg atgcttctgc ttctgagatg atacaaagaa 13261 tttagaccat aaaacagtag gttgcacaag caatagaata tggcctaaag tgttctgaca 13321 cttagaagcc aagcagtgta ggcttcttaa gaaataccat tacaatcacc ttgctagaaa 13381 tcaagcattc tggagtggtc aagcagtgta acctgtactg taagttactt ttctgctatt 13441 tttctcccaa agcaagttct ttatgctgat atttccagtg ttaggaacta caaatattaa 13501 taagttgtct tcactctttt ctttaccaag gagggtctct tccttcatct tgatctgaag 13561 gatgaacaaa ggcttgagca gtgcgcttta gaagataaac tgcagcatga aggcccccga 13621 tgttcaccca gactacatgg acctttcgcc acacatgtcc cattccagat aaggcctggc 13681 acacacaaaa aacataagtc attaggctac cagtctgatt ctaaaacaac ctaaaatctt 13741 cccacttaaa tgctatgggt ggtgggttgg aaagttgact cagaaaatca cttgctgttt 13801 ttagagagga tctgggttca gtttctgata cattgtggct tacaactata actccagttc 13861 tagggggtcc atccaacatc ctcttctgtt gagggcacca aataaatgta ttgtgtacaa 13921 a //