Inclusion Criteria: 1. Have a diagnosis of AAT-deficiency, as defined by a serum AAT level of less than 11 µM and a phenotype or genotype either homozygous for PI\*Z or compound heterozygous consisting of PI\*Z and another allele known to be associated with disease 2. Be at least 18 and not more than 75 years of age 3. Have a forced expiratory volume at one second (FEV1) \>25% of predicted value (post bronchodilator) 4. Weigh ≤ 90 kg 5. Not receiving AAT augmentation therapy currently or with the past 3 months, and not planning to begin such therapy for at least 12 months after administration of rAAV1-CB-hAAT 6. Be willing to discontinue aspirin, aspirin-containing products, and other drugs that may alter platelet function, 7 days prior to dosing, resuming no earlier than 24 hours after the dose has been administered 7. Have acceptable laboratory parameters: * Hemoglobin ≥ 11.2 g/dL for females, ≥ 12.8 g/dL for males, * White blood cell count 3,300 - 12,000 cells/mm3, * Platelet count 125,000 - 550,000/mm3, * Serum creatine kinase (CK) ≤ 3 times upper normal range for study laboratory, * Alanine aminotransferase (ALT) ≤ 2 times upper normal range for study laboratory, * Serum bilirubin ≤ 1.5 times upper normal range for study laboratory, * Serum creatinine within normal range for study laboratory, * Prothrombin time (PT) ≤ 14.5 seconds and partial thromboplastin time (PTT) ≤ 36 seconds, * Normal urine dipstick (negative glucose, negative hemoglobin, and negative or trace protein), 8. For females of childbearing potential: * A negative pregnancy test (urine or serum) at screening and at baseline (within 2 days before administration of study agent) * Agreement to consistently use barrier contraception (condoms, diaphragm or cervical cap with spermicide) or another form of contraception (e.g. intrauterine device or hormonal contraception) from the screening visit until 12 months after administration of rAAV1-CB-hAAT, for sexual activity that could lead to pregnancy 9. For males of reproductive potential, agreement to consistently use barrier contraception (condoms with spermicide) for 12 months after administration of rAAV1-CB-hAAT, for sexual activity that could lead to pregnancy, 10. Provide signed informed consent before screening Exclusion Criteria: 1. Prior receipt of any AAV gene therapy product 2. Use of anticoagulants or anti-platelet agents within 7 days prior to study agent administration 3. History of immune response to human AAT augmentation therapy as indicated by clinical history of an adverse immune response to infusion and/or decreased therapeutic effect in combination with documentation of serum anti-AAT antibodies 4. Use of acute oral or intravenous antibiotic therapy for a respiratory infection within 28 days prior to study agent administration (long-term maintenance or chronic suppressive oral antibiotics, and antibiotics for a non-respiratory indication, are allowed) 5. Use of oral or systemic corticosteroids within 28 days prior to study agent administration 6. Use of any investigational agent, or any immunosuppressive drug(s), within 3 months prior to enrollment 7. For females of childbearing potential, a positive pregnancy test at screening or baseline (within 2 days before rAAV1-CB-hAAT administration) Note: At the Cincinnati Children's Hospital Medical Center site, women of childbearing potential were not permitted to enroll in the study. 8. Females who are breast feeding 9. Have a significant abnormal EKG finding at screening and/or cardiac disease (e.g. recent myocardial infarction or CHF) within past 6 months 10. Have had pulmonary edema or a pulmonary embolism within the past 6 months 11. Have a history of immunodeficiency or other medical condition which leads the investigator to believe that the participant cannot comply with the protocol requirements or that may place the participant at an unacceptable risk for participation