Inclusion Criteria: • Participants less than or equal to (\<=) 50 years of age at the time of consent or assent (as applicable), and able to provide written consent (adults, or legal guardians, as applicable) or assent (adolescents or children). Provided that the data monitoring committee (DMC) has approved enrolling participants younger than 5 years of age, participants younger than 5 years of age may be enrolled if they weigh a minimum of 6 kilograms (kg) and are reasonably anticipated to be able to provide at least the minimum number of cells required to initiate the manufacturing process. * Diagnosis of TDT with a history of at least 100 milliliter per kilogram per year (mL/kg/year) of pRBCs in the 2 years preceding enrollment (all participants), or be managed under standard thalassemia guidelines with \>= 8 transfusions of pRBCs per year in the 2 years preceding enrollment (participants \>=12 years). * Clinically stable and eligible to undergo HSCT. * Treated and followed for at least the past 2 years in a specialized center that maintained detailed medical records, including transfusion history. Exclusion Criteria: * Presence of a mutation characterized as other then β0 (e.g., β+, βE, βC) on at least one β-globin gene (HBB) allele. * Positive for presence of human immunodeficiency virus type 1 or 2 (HIV-1 and HIV-2), hepatitis B virus (HBV), or hepatitis C (HCV). * A white blood cell (WBC) count less than (\<) 3×10\^9/liter (L), and/or platelet count \<100×10\^9/L not related to hypersplenism. * Uncorrected bleeding disorder. * Any prior or current malignancy. * Prior HSCT. * Advanced liver disease. * A cardiac T2\* \<10 ms by MRI. * Any other evidence of severe iron overload that, in the investigator's opinion, warrants exclusion. * Participation in another clinical study with an investigational drug within 30 days of Screening. * Any other condition that would render the participant ineligible for HSCT, as determined by the attending transplant physician or investigator. * Prior receipt of gene therapy. * Pregnancy or breastfeeding in a postpartum female or absence of adequate contraception for fertile participant. * A known and available human leukocyte antigen (HLA) matched family donor. * Any contraindications to the use of granulocyte colony stimulating factor (G-CSF) and plerixafor during the mobilization of hematopoietic stem cells and any contraindications to the use of busulfan and any other medicinal products required during the myeloablative conditioning, including hypersensitivity to the active substances or to any of the excipients.