Inclusion Criteria: 1. Subject was male, 16 years of age or older (18 years of age or older in the US), and post pubertal,(minimum age by region) 2. Subject had a confirmed diagnosis of classic Fabry disease based on deficient AGA enzyme activity (defined as \< 1% of normal). Exclusion Criteria: 1. Subject had a galactosidase alpha (GLA) gene mutation associated with late-onset cardiac variant Fabry disease. 2. Subject had previously received ERT and/or chaperone therapy within 3 years for treatment of Fabry disease. 3. Subject had tested positive for anti-AGA antibodies at the time of screening. 4. Subject had eGFR \< 60 mL/min/1.73 m² (ie, chronic kidney disease \[CKD\] stage ≥ 3) at Screening. 5. Subject had a prior history of myocardial infarction (MI). 6. Subject had a history of coronary artery disease (CAD) with angina requiring percutaneous transluminal coronary angioplasty (with or without stent placement) and/or coronary artery bypass graft (CABG). 7. Subject had a history of moderate to severe valvular heart disease requiring valve replacement. 8. Subject had a history of heart failure, moderate to severe diastolic dysfunction, and/or left ventricular ejection fraction (LVEF) ≤ 45% on echocardiogram (ECHO) performed at rest at Screening. 9. Subject had a history of clinically significant cardiac arrhythmia (eg, heart block \[second or third degree\], atrial fibrillation requiring therapy, ventricular fibrillation, ventricular tachycardia, supraventricular tachycardia, or cardiac arrest). Note \[history of intermittent atrial fibrillation not requiring treatment was allowed\]. 10. Subject had a prior history of stroke and/or transient ischemic attack (TIA). 11. Subject had aspartate aminotransferase (AST) and/or alanine aminotransferase (ALT) ≥ 3 times the upper limit of normal (ULN) at Screening. 12. Subject had a prior history of (or current) malignancy; the one exception is a prior history of resected basal cell carcinoma. 13. Subject had previously received treatment with AVR-RD-01 or any other gene therapy. Other inclusion/exclusion criteria apply.