Inclusion Criteria: 1. SMA was diagnosed by a bilaterally allelic SMN1 mutation (deletion or point mutation) gene with 2 copies of the SMN2 gene. 2. On the day of dosing, the subject ' s age did not exceed postnatal Day 180. 3. The clinical history and signs were consistent with type 1 SMA manifestations, i.e. hypotonia, delayed motor function development, poor head control, round shoulder posture, and joint hypermobility. 4. The subject's legal guardian understands the purpose, possible risks and interests of the study, agrees to participate in the study, completes all study procedures, tests and visits, and voluntarily signs the informed consent form. 5. During the study, the subject's legal guardian was willing to perform standard treatment requirements such as nasogastric feeding, noninvasive mechanical ventilation, and expectoration machine as recommended by the investigator. Exclusion Criteria: 1. Gestational age at birth was less than 35 weeks (245 days). 2. At screening, the subject had an oxygen saturation \< 96% while awake or sleeping and did not receive any supplemental oxygen or respiratory support. 3. Requirement of invasive ventilation or tracheotomy, or current use of noninvasive ventilatory support for an average of ≥ 16 hours/day. 4. Weighed below the 3rd percentile by age according to the WHO Child Growth Criteria (WHO 2009). 5. Before administration, if the subject has not received or delayed vaccination according to the current month-old national vaccination plan, it will significantly affect the safety of the subject as assessed by the investigator and the medical manager of the project team; 6. Active viral infections (including HIV, COVID-19, hepatitis B or C seropositivity, torch virus, Epstein-Barr virus, and syphilis). 7. Serious non-respiratory disease within 2 weeks prior to screening. 8. Upper respiratory tract infection or lower respiratory tract infection within 4 weeks prior to screening. 9. Current presence of other severe infections or diseases. 10. Known cardiac disease or ECG abnormalities that are clinically significant. 11. Known hypersensitivity to prednisolone, other glucocorticoids, or its excipients. 12. Immunosuppressive therapy (eg, cyclosporine, tacrolimus, methotrexate, cyclophosphamide, rituximab) other than protocol-required prophylaxis within 3 months prior to dosing. 13. Immunomodulatory drugs (eg, thymosin, interferon, etc.) are being used to treat myopathy, neuritis, diabetes mellitus (eg, immunosuppressants, glucocorticoids, insulin). 14. Anti-AAV9 antibody titer \> 1: 50 (as determined by ECL). If the potential subject has an anti-AAV9 antibody titer \> 1: 50, it can be retested during the screening period. If the anti-AAV9 antibody titer is ≤ 1: 50 at the retest, the subject may continue to participate in the screening. 15. Clinically significant abnormal laboratory values (GGT, ALT, and AST \> 2.5 × ULN, bilirubin ≥ 3.0 mg/dL, creatinine ≥ 1.0 mg/dL, hemoglobin \< 8 or \> 18 g/dL; white blood cell count \> 20,000/cm3; platelet count \< 100,000/cm3). 16. Prior use of other SMA therapeutic agents (e.g., nosinasenat, rispolam, and Zolgensma, etc.) or participated in clinical studies with other SMA therapeutic agents (including but not limited to the above 3 drugs). 17. Major surgery is expected during study treatment. 18. Other circumstances that, in the judgment of the investigator, are not suitable for participation in this study.