Contact SCGE
Your email
Message
Send
Development System
STS119 (TLR-MCV1b) (20 pmol RNP) | SCGE Toolkit
Database of study and experiment data generated by the Somatic Cell Genome Editing Consortium
SCGE Consortium Home
|
About SCGE Toolkit
|
Contact Us
|
Citing the Toolkit
|
License
Examples:
Epithelium
,
CRISPR
,
AAV
,
Ai9
Login
Home
Explore Data
(current)
Explore Projects
(current)
Genome Editors
(current)
Model Systems
Delivery Systems
Guides
Vectors
Protocols
Publications
Home
/
Project
/
Experiment
Experiment Record Detail
Experiment
Testing newly chemically modified crRNA and tracrRNA to activate the TLR reporter in human cells
Experiment Condition
STS119 (TLR-MCV1b) (20 pmol RNP)
Assay Description:
Calculated editing efficiency by flow cytometry analysis of RFP expression.
Tissue Measured:
Measurement Type:
Editing Efficiency
Record ID
15000000549
Editor
SpyCas9-3xNLS
Delivery System
Delivery System Subtype
Guide
STS119 (TLR-MCV1b; Sp_t2:Sp_c20_TLR_MCV1b)
Vector
Model Species
Human
Model Name
HEK-293T-disrupted_GFP with MCV-mcherry-Puro
Application Method
electroporation
Application Site
Dosage
20 pmol RNP
Injection Frequency
Injection Rate
Injection Volume
Days post injection
48-72 hours
Editor Format
protein, RNA
Antidote Id
Antidote Description
Measured Values
Samples Size:
2
Replicate
Result (Editing Efficiency)
Measurement Units:
% of RFP+ cells
Mean
22.3 
1
23 
2
21.6