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ExperimentTesting newly chemically modified crRNA and tracrRNA to activate the TLR reporter in human cells
Experiment ConditionSTS119 (TLR-MCV1b) (20 pmol RNP)
Assay Description: Calculated editing efficiency by flow cytometry analysis of RFP expression.
Tissue Measured:
Measurement Type:Editing Efficiency
Record ID15000000549

EditorSpyCas9-3xNLS
Delivery System
Delivery System Subtype
GuideSTS119 (TLR-MCV1b; Sp_t2:Sp_c20_TLR_MCV1b)
Vector

Model SpeciesHuman
Model NameHEK-293T-disrupted_GFP with MCV-mcherry-Puro

Application Methodelectroporation
Application Site
Dosage20 pmol RNP
Injection Frequency
Injection Rate
Injection Volume
Days post injection48-72 hours
Editor Formatprotein, RNA
Antidote Id
Antidote Description

Measured Values
Samples Size: 2
 
Replicate Result (Editing Efficiency)
Measurement Units: % of RFP+ cells
Mean 22.3 
1 23 
2 21.6