25 results for ai9
25 results for ai9
Ai14 gRNA - Guide [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo] |
Ai9 mouse (BCM) - Model System [Small Animal Testing Center (SATC)] - [In Vivo] |
Ai14 mouse - Model System [Delivery Systems Initiative] - [In Vivo] |
Ai9 SaCas9 Guide A - Guide [Small Animal Testing Center (SATC)] - [In Vivo] |
Ai14 mouse primary fibroblasts - Model System [Delivery Systems Initiative] - [In Vitro] |
Ai14 mouse (congenic) - Model System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo]
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Ai9 mouse - Model System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo]
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Ai9 SaCas9 Guide B - Guide [Small Animal Testing Center (SATC)] - [In Vivo] |
Ai9 mouse immortalized fibroblasts - Model System [Delivery Systems Initiative] - [In Vitro] |
Ai9-SauSpyCas9 mouse - Model System [Small Animal Testing Center (SATC)] - [In Vivo] |
Other Id:
ab150155
Abcam, Donkey anti-rat alexa fluour 647 |
[Validation] Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain - Experiment [Small Animal Testing Center (SATC)] - [In Vivo] |
Other Id:
13-0300Â
Thermo-Fisher, Rat anti-GFAP |
Other Id:
A21207
Invitrogen, Donkey anti-rabbit alexa fluor 594 |
Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. - Experiment [Delivery Systems Initiative] - [In Vivo] |
Other Id:
Rockland Cat# 600-401-379
Anti-RFP (RABBIT) Antibody |
Other Id:
ab104224Â
Abcam, mouse anti-NeuN |
RNP-NC-no ligand - Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo] |
sNLS-SpCas9-sNLS - Genome Editor [Small Animal Testing Center (SATC), Delivery Systems Initiative] |
RNP-NC-CPP - Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo] |
RNP-NC-RVG - Delivery System [Small Animal Testing Center (SATC)] - [In Vivo] |
Other Id:
A21202
Invitrogen, Donkey anti-mouse alexafluor 488 |
AAVcc47-SaCas9-Ai9 - Vector [Small Animal Testing Center (SATC)] |
[Validation] Validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing. - Experiment [Small Animal Testing Center (SATC)] - [In Vivo] |
SauCas9 - Genome Editor [Small Animal Testing Center (SATC)] |
25 results for ai9
Category | Type | Name | Description | View Associated.. |
---|---|---|---|---|
Guide | Ai14 gRNA | This sgRNA targets the Ai9 and related transgenes at multiple sites | ||
Model System | Animal | Ai9 mouse (BCM) | Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. | |
Model System | Animal | Ai14 mouse | Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain. | |
Guide | Ai9 SaCas9 Guide A | This gRNA targets the Ai9 and related transgenes | ||
Model System | Cell | Ai14 mouse primary fibroblasts | Primary fibroblasts from Ai14 mice | |
Model System | Animal | Ai14 mouse (congenic) | Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain. |
|
Model System | Animal | Ai9 mouse | Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. |
|
Guide | Ai9 SaCas9 Guide B | This gRNA targets the Ai9 and related transgenes | ||
Model System | Cell | Ai9 mouse immortalized fibroblasts | Immortalized fibroblasts made from Ai9 (B6.Cg-Gt(ROSA)26Sor^tm9(CAG-tdTomato)Hze/J) mice | |
Model System | Animal | Ai9-SauSpyCas9 mouse | Ai9-SauSpyCas9 is a version of Ai9 that has a single guide RNA sites on both sides of the STOP cassette. This is a Cre reporter allele that has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. Ai9 mice express robust tdTomato fluorescence following Cre-mediated recombination. | |
Antibody | AB_2813835 | Abcam, Donkey anti-rat alexa fluour 647 | ||
Experiment | In Vivo | Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain | Delivery of CRISPR/Cas9 via RNP-loaded nanocages to the brain in Ai14 mice | |
Antibody | AB_2532994 | Thermo-Fisher, Rat anti-GFAP | ||
Antibody | AB_141637 | Invitrogen, Donkey anti-rabbit alexa fluor 594 | ||
Experiment | In Vivo | Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. | Nanocapusules carrying CRISPR Cas9 RNP with guide RNA targeting the stop sequence in the Ai14 transgene are intracerebrally delivered to Ai14 mice and gene editing is measured by gain of tdTomato protein expression. | |
Antibody | AB_2209751 | Anti-RFP (RABBIT) Antibody | ||
Antibody | AB_10711040 | Abcam, mouse anti-NeuN | ||
Delivery System | Nanoparticle | RNP-NC-no ligand | The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. | |
Genome Editor | Cas Nuclease | sNLS-SpCas9-sNLS | SpCas9 with N- and C-terminal SV40 NLS | |
Delivery System | Nanoparticle | RNP-NC-CPP | The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a cell penetrating peptide (CPP) from the TAT peptide (GRKKRRQRRRPQ) which lacks cell-type specficity | |
Delivery System | Nanoparticle | RNP-NC-RVG | The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a RVG peptide YTIWMPENPRPGTPCDIFTNSRGKRASNG which specifically interacts withthe N-acetylecholine receptor (AchR) on neuronal cells, which mediates NP entry | |
Antibody | AB_141607 | Invitrogen, Donkey anti-mouse alexafluor 488 | ||
Vector | Viral Vector | AAVcc47-SaCas9-Ai9 | AAV2/9 expressing SaCas9 and single sgRNA under U6 promoter | |
Experiment | In Vivo | Validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing. | Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery. Detection of editing in non-target tissues. | |
Genome Editor | Cas Nuclease | SauCas9 |