115 results for ai9

Ai9 mouse   - Model System - [In Vivo]
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) description ->  Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent generatedDescription ->  ..................................................HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 ; and related transgenes ..This gRNA targets the Ai9 and related transgenes ..AAV serotype 9 delivering ; u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus..AAVcc47 delivering sgRNA 2 + CB SaCas9 ; HA-SV40NLS-SpCas9-SV40NLS..AAV serotype 9 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus..AAV ; serotype 9 delivering gRNA 2 + CB SaCas9 targeting the Ai9 locus..Novel engineered AAV BI28 variant Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene experimentName ->  Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Cre Recombinase dose escalation study in Ai9 mice
Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus.
View Associated: 
Ai9 mouse (BCM)   - Model System - [In Vivo]
Matched on: Name ->  Ai9 mouse (BCM) modelName ->  Ai9 mouse (BCM) transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) grnaLabId ->  Ai9 SaCas9 Guide A ; Ai9 SaCas9 Guide B description ->  Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent generatedDescription ->  AAVcc47-Cre viral vector..Cre recombinase delivered by AAVcc47-Cre viral vector..This sgRNA targets the Ai9 ; and related transgenes ..This gRNA targets the Ai9 and related transgenes ..AAV2/9 expressing SaCas9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus.
View Associated: 
Ai9 mouse immortalized fibroblasts   - Model System - [In Vitro]
Matched on: Name ->  Ai9 mouse immortalized fibroblasts modelName ->  Ai9 mouse immortalized fibroblasts transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) description ->  Immortalized fibroblasts made from Ai9 (B6.Cg-Gt(ROSA)26Sor^tm9(CAG-tdTomato)Hze/J) mice generatedDescription ->  pAAV-EF1a-Cre plasmid..Cre recombinase delivered by pAAV-EF1a-Cre plasmid..This gRNA targets the mTmG, Ai9 ; and related transgenes at two sites..This gRNA targets the Ai9 and related transgenes ..AAV shuttle ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene ; Ai9/Ai14/mTmG and related reporter transgene (within loxP) guideTargetLocus ->  Ai9/Ai14 reporter transgene ; Ai9/Ai14/mTmG and related reporter transgene (within loxP) experimentName ->  Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus
Immortalized fibroblasts made from Ai9 (B6.Cg-Gt(ROSA)26Sor^tm9(CAG-tdTomato)Hze/J) mice
View Associated: 
Ai9 SaCas9 Guide B   - Guide
Matched on: Name ->  Ai9 SaCas9 Guide B description ->  This gRNA targets the Ai9 and related transgenes
This gRNA targets the Ai9 and related transgenes
Ai9 SaCas9 Guide A   - Guide
Matched on: Name ->  Ai9 SaCas9 Guide A description ->  This gRNA targets the Ai9 and related transgenes
This gRNA targets the Ai9 and related transgenes
HEK-293T with Ai9 transient reporter assay   - Model System - [In Vitro]
Matched on: Name ->  HEK-293T with Ai9 transient reporter assay modelName ->  HEK-293T with Ai9 transient reporter assay transgene ->  Ai9 plasmid description ->  HEK-293T cells transfected with an Ai9 inducible transgene reporter plasmid used to test gene editing generatedDescription ->  recombinase delivered by scAAV5-Cre-GFP plasmid..HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 ; HA-SV40NLS-SpCas9-SV40NLS..Lipid nanoparticle..HEK-293T cells transfected with an Ai9 inducible transgene Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
HEK-293T cells transfected with an Ai9 inducible transgene reporter plasmid used to test gene editing activity by fluorescence
View Associated: 
Cre Recombinase dose escalation study in Ai9 mice   - Experiment - [In Vivo]
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) description ->  A single stranded cmv cre cassette was packaged into AAV9 or AAVcc47 and injected intravenously in Ai9 generatedDescription ->  A single stranded cmv cre cassette was packaged into AAV9 or AAVcc47 and injected intravenously in Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName ->  Cre Recombinase dose escalation study in Ai9 mice
Asokan Aravind
A single stranded cmv cre cassette was packaged into AAV9 or AAVcc47 and injected intravenously in Ai9 mice. We injected n=3 at three different doses (1e10, 1e11, 1e12 vg) and harvested organs 4 weeks post injection. Fluorescence intensity in liver, heart, and skeletal muscle was quantified with tiff based images in Image J and neuronal transduction from each vector was quantified at the 1e12vg dose by counting the number of tdTomato+ neurons and number of NeuN+ cells from multiple sections and images.
View Associated: 
Testing gRNA sequence and gRNA scaffold modified in Ai9 mice.   - Experiment - [In Vivo]
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) description ->  /mouse of AAV-BI28:GFAP-NLS-GFP-U6-L1-U6-R2 were codelivered intravenously to adult male and female Ai9 generatedDescription ->  /mouse of AAV-BI28:GFAP-NLS-GFP-U6-L1-U6-R2 were codelivered intravenously to adult male and female Ai9 ; Editing was assessed in brain sections 4 weeks later. ..............This gRNA targets the Ai9 and related ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 ; variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus.................This gRNA targets the Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene experimentName ->  Testing gRNA sequence and gRNA scaffold modified in Ai9 mice.
Deverman Benjamin E
3e11 vg/mouse of AAV-BI28:GFAP-SaCas9-WPRE-pA and 3e11 vg/mouse of AAV-BI28:GFAP-NLS-GFP-U6-L1-U6-R2 were codelivered intravenously to adult male and female Ai9 mice. Editing was assessed in brain sections 4 weeks later.
View Associated: 
Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus in vitro   - Experiment - [In Vitro]
Matched on: Name ->  Ai9 mouse immortalized fibroblasts modelName ->  Ai9 mouse immortalized fibroblasts transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) description ->  activation by SaCas9 gRNA target and modified scaffold sequences by transient transfection in immortalized Ai9 generatedDescription ->  activation by SaCas9 gRNA target and modified scaffold sequences by transient transfection in immortalized Ai9 ; pAAV-EF1a-Cre plasmid..Cre recombinase delivered by pAAV-EF1a-Cre plasmid..This gRNA targets the mTmG, Ai9 ; and related transgenes at two sites..This gRNA targets the Ai9 and related transgenes ..AAV shuttle ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 ; and related transgenes at two sites..This gRNA targets the Ai9 and related transgenes ..AAV shuttle Guide Target Locus ->  Ai9/Ai14 reporter transgene ; Ai9/Ai14/mTmG and related reporter transgene (within loxP) guideTargetLocus ->  Ai9/Ai14 reporter transgene ; Ai9/Ai14/mTmG and related reporter transgene (within loxP) experimentName ->  Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus
Deverman Benjamin E
Reporter transgene activation by SaCas9 gRNA target and modified scaffold sequences by transient transfection in immortalized Ai9 mouse fibroblasts
View Associated: 
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter)   - Experiment - [In Vivo]
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) description ->  This strategy was evaluted with both AAV9 (n=3) and AAVcc47 (n=3) by intravenous injection in Ai9 mice generatedDescription ->  This strategy was evaluted with both AAV9 (n=3) and AAVcc47 (n=3) by intravenous injection in Ai9 mice ; . ....................AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 ; .......................AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to
Asokan Aravind
A dual vector strategy was employed: one delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (both single stranded AAV cassettes). This strategy was evaluted with both AAV9 (n=3) and AAVcc47 (n=3) by intravenous injection in Ai9 mice. A total dose of 3e12vg was injected into each mouse (1.5e12vg each vector mixed 1:1) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
View Associated: 
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter)   - Experiment - [In Vivo]
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) description ->  This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice generatedDescription ->  This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice ; locus..AAV serotype 9 delivering gRNA 2 + CB SaCas9 targeting the Ai9 locus..AAVcc47 delivering sgRNA ; 1 + CB SaCas9 targeting the Ai9 locus..AAV serotype 9 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 ; locus..AAV serotype 9 delivering gRNA 2 + CB SaCas9 targeting the Ai9 locus..AAVcc47 delivering sgRNA ; 1 + CB SaCas9 targeting the Ai9 locus..AAV serotype 9 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
Asokan Aravind
A dual vector strategy was employed: one delivering a single guide RNA and CB driven SaCas9, and another delivering the second guide RNA and CB driven SaCas9. This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice. A total dose of 2e12vg was injected into each mouse (1e12vg each vector mixed 1:1) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
View Associated: 
Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter)   - Experiment - [In Vivo]
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) description ->  This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice generatedDescription ->  This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice ; .......................AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 ; .......................AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName ->  Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
Asokan Aravind
A dual vector strategy was employed: one delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (both single stranded AAV cassettes). This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice. A total dose of 4e12vg was injected into each mouse and vectors mixed in a 1:4 ratio of cas9 to guide RNA (1e12vg of CMV Sacas9 vector and 3e12vg of the sgRNA vector) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
View Associated: 
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.   - Experiment - [In Vivo]
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) description ->  This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=4) by intravenous injection in Ai9 mice generatedDescription ->  This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=4) by intravenous injection in Ai9 mice ; AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 ; SaCas9..AAVcc47 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 ; AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 ; SaCas9..AAVcc47 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
Asokan Aravind
A dual vector strategy was employed: one self complementary vector delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (single stranded vector). This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=4) by intravenous injection in Ai9 mice. A total dose of 4e12vg was injected into each mouse and vectors mixed in a 1:1 ratio of cas9 to guide RNA (2e12vg of CMV Sacas9 vector and 2e12vg of the self complementary sgRNA vector) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
View Associated: 
[] Validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing.   - Experiment - [In Vivo]
Matched on: Name ->  Ai9 mouse (BCM) modelName ->  Ai9 mouse (BCM) transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) grnaLabId ->  Ai9 SaCas9 Guide A ; Ai9 SaCas9 Guide B generatedDescription ->  AAVcc47-Cre viral vector..Cre recombinase delivered by AAVcc47-Cre viral vector..This gRNA targets the Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 ; AAVcc47-Cre viral vector..Cre recombinase delivered by AAVcc47-Cre viral vector..This gRNA targets the Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Heaney Jason D
Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery. Detection of editing in non-target tissues.
View Associated: 
SauCas9   - Genome Editor
Matched on: Name ->  Ai9 mouse (BCM) modelName ->  Ai9 mouse (BCM) transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) grnaLabId ->  Ai9 SaCas9 Guide A ; Ai9 SaCas9 Guide B generatedDescription ->  ..............................................................................This gRNA targets the Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
View Associated: 
AAVcc47-SaCas9-Ai9   - Vector
Matched on: Name ->  Ai9 mouse (BCM) modelName ->  Ai9 mouse (BCM) transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) grnaLabId ->  Ai9 SaCas9 Guide A ; Ai9 SaCas9 Guide B generatedDescription ->  ..............................................................................This gRNA targets the Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
AAV2/9 expressing SaCas9 and single sgRNA under U6 promoter
View Associated: 
SaCas9   - Genome Editor
Matched on: Name ->  Ai9 mouse immortalized fibroblasts ; Ai9 mouse modelName ->  Ai9 mouse immortalized fibroblasts ; Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  ........................................................................This gRNA targets the mTmG, Ai9 ; and related transgenes at two sites..This gRNA targets the Ai9 and related transgenes ..AAV shuttle ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 ; variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus...Immortalized fibroblasts made from Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene ; Ai9/Ai14/mTmG and related reporter transgene (within loxP) guideTargetLocus ->  Ai9/Ai14 reporter transgene ; Ai9/Ai14/mTmG and related reporter transgene (within loxP) experimentName ->  Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. ; Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus
View Associated: 
AAVcc47-CMV-Cre   - Vector
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName ->  Cre Recombinase dose escalation study in Ai9 mice
AAVcc47 delivering CMV Cre Recombinase
View Associated: 
Testing AAV5 for activation of tdTomato in mouse airway   - Experiment - [In Vivo]
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) description ->  mediated gene editing in the mouse airway was tested by deliverying SpCas9 and guide RNAs targeting the Ai9 ; transgene in Ai9 transgenic mice. generatedDescription ->  mediated gene editing in the mouse airway was tested by deliverying SpCas9 and guide RNAs targeting the Ai9 ; transgene in Ai9 transgenic mice. ; gene editing quantified by tdTomato activation ..HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 ; RABBIT) Antibody..GFP (D5.1) XP Rabbit mAb antibody..HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Gao Guang-Ping
AAV2/5 mediated gene editing in the mouse airway was tested by deliverying SpCas9 and guide RNAs targeting the Ai9 transgene in Ai9 transgenic mice. Viral delivery was detected by GFP expression and gene editing quantified by tdTomato activation
View Associated: 
[] Second site validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain   - Experiment - [In Vivo]
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) description ->  Editing detected and quantified in brain by generation of tdTomato fluorescent protein signal from Ai9 generatedDescription ->  Editing detected and quantified in brain by generation of tdTomato fluorescent protein signal from Ai9 ; reporter mice..................................This gRNA targets the Ai9 and related transgenes ..Novel ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 ; all inserted into the Gt(ROSA)26Sor locus.....................................This gRNA targets the Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Heaney Jason D
Validation of delivery of AAV custom designed to cross the blood-brain barrier for CRISPR/Cas9 editing. Editing detected and quantified in brain by generation of tdTomato fluorescent protein signal from Ai9 reporter mice
View Associated: 
AAV9-Ai9-sgRNA1-CB-SaCas9   - Vector
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) description ->  AAV serotype 9 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus generatedDescription ->  ..........AAV serotype 9 delivering gRNA 2 + CB SaCas9 targeting the Ai9 locus..AAV serotype 9 delivering ; sgRNA 1 + CB SaCas9 targeting the Ai9 locus....CAG-loxP-stop-loxP-tdtomato reporter cassette which is ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAV serotype 9 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus
View Associated: 
AAV9-Ai9-sgRNA2-CB-SaCas9   - Vector
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) description ->  AAV serotype 9 delivering gRNA 2 + CB SaCas9 targeting the Ai9 locus generatedDescription ->  ..........AAV serotype 9 delivering gRNA 2 + CB SaCas9 targeting the Ai9 locus..AAV serotype 9 delivering ; sgRNA 1 + CB SaCas9 targeting the Ai9 locus....CAG-loxP-stop-loxP-tdtomato reporter cassette which is ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAV serotype 9 delivering gRNA 2 + CB SaCas9 targeting the Ai9 locus
View Associated: 
AAVcc47-Ai9-sgRNA1-CB-SaCas9   - Vector
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) description ->  AAVcc47 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus generatedDescription ->  ..........AAVcc47 delivering sgRNA 2 + CB SaCas9 targeting the Ai9 locus..AAVcc47 delivering sgRNA 1 ; + CB SaCas9 targeting the Ai9 locus....CAG-loxP-stop-loxP-tdtomato reporter cassette which is activated ; by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAVcc47 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus
View Associated: 
AAVcc47-Ai9-sgRNA1 + sgRNA2   - Vector
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) description ->  AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus generatedDescription ->  ..............AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus. ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus
View Associated: 
g-loxP2_C9   - Guide - [In Vivo]
Matched on: description ->  This sgRNA targets the Ai9 and related transgenes generatedDescription ->  This sgRNA targets the Ai9 and related transgenes ....Shuttle peptide used to deliver reagents to airway Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
This sgRNA targets the Ai9 and related transgenes
View Associated: 
Cre recombinase   - Genome Editor
Matched on: Name ->  Ai9 mouse (BCM) modelName ->  Ai9 mouse (BCM) transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9
Cre recombinase delivered by AAVcc47-Cre viral vector
View Associated: 
AAVcc47_pTR_self comp 2xU6-Ai9 guides   - Vector
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) description ->  AAVcc47 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 transgene generatedDescription ->  SaCas9..AAVcc47 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAVcc47 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 transgene
View Associated: 
AB_1196615    - Antibody - [In Vivo]
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 and related transgenes ..AAV2/5 expressing SpyCas9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
GFP (D5.1) XP Rabbit mAb antibody
View Associated: 
AAV-CMV-SaCas9-U6-modified scaffold-Sa-R2   - Vector
Matched on: Name ->  Ai9 mouse immortalized fibroblasts modelName ->  Ai9 mouse immortalized fibroblasts transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  ........This gRNA targets the Ai9 and related transgenes ..AAV shuttle vector with CMV promoter driven ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene experimentName ->  Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus
AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA with modified scaffold sequence
View Associated: 
L2-modified   - Guide
Matched on: description ->  This gRNA targets the Ai9 and related transgenes
This gRNA targets the Ai9 and related transgenes
L3-unmodified   - Guide
Matched on: description ->  This gRNA targets the Ai9 and related transgenes
This gRNA targets the Ai9 and related transgenes
R1-modified   - Guide
Matched on: description ->  This gRNA targets the Ai9 and related transgenes
This gRNA targets the Ai9 and related transgenes
sgAi9L   - Guide - [In Vitro]
Matched on: Name ->  HEK-293T with Ai9 transient reporter assay modelName ->  HEK-293T with Ai9 transient reporter assay transgene ->  Ai9 plasmid description ->  This sgRNA targets the Ai9 and related transgenes generatedDescription ->  This sgRNA targets the Ai9 and related transgenes ..AAV2/5 expressing SpyCas9. ; HA-SV40NLS-SpCas9-SV40NLS..Lipid nanoparticle..HEK-293T cells transfected with an Ai9 inducible transgene Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
This sgRNA targets the Ai9 and related transgenes
View Associated: 
sgAi9L   - Guide
Matched on: description ->  This sgRNA targets the Ai9 and related transgenes
This sgRNA targets the Ai9 and related transgenes
sgAi9R   - Guide - [In Vitro]
Matched on: Name ->  HEK-293T with Ai9 transient reporter assay modelName ->  HEK-293T with Ai9 transient reporter assay transgene ->  Ai9 plasmid description ->  This sgRNA targets the Ai9 and related transgenes generatedDescription ->  This sgRNA targets the Ai9 and related transgenes ..AAV2/5 expressing SpyCas9. ; HA-SV40NLS-SpCas9-SV40NLS..Lipid nanoparticle..HEK-293T cells transfected with an Ai9 inducible transgene Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
This sgRNA targets the Ai9 and related transgenes
View Associated: 
SaCas9-Lagor   - Genome Editor
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  .......................AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 ; locus..AAVcc47 delivering sgRNA 2 + CB SaCas9 targeting the Ai9 locus..AAV serotype 9 delivering gRNA ; 2 + CB SaCas9 targeting the Ai9 locus..AAVcc47 delivering CMV driven SaCas9..AAV serotype 9 delivering ; u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 transgene..AAV serotype ; 9 delivering CMV driven SaCas9..AAVcc47 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus..AAV experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
View Associated: 
AAV9-CMV-Cre   - Vector
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName ->  Cre Recombinase dose escalation study in Ai9 mice
AAV serotype 9 delivering CMV Cre Recombinase
View Associated: 
AAV9_pTR_self comp 2xU6-Ai9 guides   - Vector
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) description ->  AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 generatedDescription ->  AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 transgene
View Associated: 
AAV.pU1a-SpCas9   - Vector
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 and related transgenes ..AAV2/5 expressing SpyCas9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Expresses codon-optimized SpCas9 in mammalian cells. HA-SV40NLS-SpCas9-SV40NLS
View Associated: 
H509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1)   - Vector
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 and related transgenes ..AAV2/5 expressing SpyCas9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
AAV2/5 expressing SpyCas9. AAV2/5 expressing two sgRNAs under U6 promoter and eGFP
View Associated: 
Testing AAV5 for activation of tdTomato in HEK293T cells   - Experiment - [In Vitro]
Matched on: Name ->  HEK-293T with Ai9 transient reporter assay modelName ->  HEK-293T with Ai9 transient reporter assay transgene ->  Ai9 plasmid description ->  AAV shuttle plasmids expressing SpCas9 and guide RNAs targeting the Ai9 transgene were tested in HEK293T generatedDescription ->  AAV shuttle plasmids expressing SpCas9 and guide RNAs targeting the Ai9 transgene were tested in HEK293T ; recombinase delivered by scAAV5-Cre-GFP plasmid..HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 ; HA-SV40NLS-SpCas9-SV40NLS..Lipid nanoparticle..HEK-293T cells transfected with an Ai9 inducible transgene ; recombinase delivered by scAAV5-Cre-GFP plasmid..HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 ; HA-SV40NLS-SpCas9-SV40NLS..Lipid nanoparticle..HEK-293T cells transfected with an Ai9 inducible transgene Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Gao Guang-Ping
AAV shuttle plasmids expressing SpCas9 and guide RNAs targeting the Ai9 transgene were tested in HEK293T cells by transient transfection. Both delivery and gene editing were detected by fluorescence.
View Associated: 
L1-modified   - Guide
Matched on: description ->  This gRNA targets the Ai9 and related transgenes
This gRNA targets the Ai9 and related transgenes
R1-unmodified   - Guide
Matched on: description ->  This gRNA targets the Ai9 and related transgenes
This gRNA targets the Ai9 and related transgenes
R2-unmodified   - Guide
Matched on: description ->  This gRNA targets the Ai9 and related transgenes
This gRNA targets the Ai9 and related transgenes
AAV-CMV-SaCas9-U6-modified scaffold-Sa-L2   - Vector
Matched on: Name ->  Ai9 mouse immortalized fibroblasts modelName ->  Ai9 mouse immortalized fibroblasts transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  ......This gRNA targets the Ai9 and related transgenes ..AAV shuttle vector with CMV promoter driven ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene experimentName ->  Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus
AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA with modified scaffold sequence
View Associated: 
AAVcc47-Cre   - Vector
Matched on: Name ->  Ai9 mouse (BCM) modelName ->  Ai9 mouse (BCM) transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9
AAV2/5 expressing Cre recombinase
View Associated: 
AAV9-Ai9-sgRNA1 + sgRNA2   - Vector
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) description ->  AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus generatedDescription ->  ..................AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus
View Associated: 
AAVcc47-Ai9-sgRNA2-CB-SaCas9   - Vector
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) description ->  AAVcc47 delivering sgRNA 2 + CB SaCas9 targeting the Ai9 locus generatedDescription ->  ..........AAVcc47 delivering sgRNA 2 + CB SaCas9 targeting the Ai9 locus..AAVcc47 delivering sgRNA 1 ; + CB SaCas9 targeting the Ai9 locus....CAG-loxP-stop-loxP-tdtomato reporter cassette which is activated ; by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAVcc47 delivering sgRNA 2 + CB SaCas9 targeting the Ai9 locus
View Associated: 
AAVcc47-CMV-SaCas9   - Vector
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  ..............AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus. ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
AAVcc47 delivering CMV driven SaCas9
View Associated: 
BI28:AAV-GFAP-NLS-GFP-WPRE-synpA-L1-R2   - Vector
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  ........................................This gRNA targets the Ai9 and related transgenes ..Novel engineered ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene experimentName ->  Testing gRNA sequence and gRNA scaffold modified in Ai9 mice.
Novel engineered AAV BI28 variant expressing NLS-GFP driven by the glial fibrillary acidic protein (GFAP) promoter and dual sgRNAs with modified scaffolds
View Associated: 
SpCas9   - Genome Editor
Matched on: Name ->  Ai9 mouse ; HEK-293T with Ai9 transient reporter assay modelName ->  Ai9 mouse ; HEK-293T with Ai9 transient reporter assay transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) ; Ai9 plasmid generatedDescription ->  HA-SV40NLS-SpCas9-SV40NLS..HA-SV40NLS-SpCas9-SV40NLS..HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 ; strand breaks on both sides of the stop cassette and NHEJ repair ..HEK-293T cells transfected with an Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
HA-SV40NLS-SpCas9-SV40NLS
View Associated: 
AB_2209751    - Antibody - [In Vivo]
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 ; and related transgenes ..This sgRNA targets the Ai9 and related transgenes at multiple sites..AAV2/5 ; The att site flanked neo selection cassette has been removed in this strain...Ai9 mouse has a loxP-flanked Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Anti-RFP (RABBIT) Antibody
View Associated: 
L1-unmodified   - Guide
Matched on: description ->  This gRNA targets the Ai9 and related transgenes
This gRNA targets the Ai9 and related transgenes
L2-unmodified   - Guide
Matched on: description ->  This gRNA targets the Ai9 and related transgenes
This gRNA targets the Ai9 and related transgenes
L3-modifed   - Guide
Matched on: description ->  This gRNA targets the Ai9 and related transgenes
This gRNA targets the Ai9 and related transgenes
R2-modified   - Guide
Matched on: description ->  This gRNA targets the Ai9 and related transgenes
This gRNA targets the Ai9 and related transgenes
AAV-CMV-SaCas9-U6-gRNA-LoxP-Sa-L3   - Vector
Matched on: Name ->  Ai9 mouse immortalized fibroblasts modelName ->  Ai9 mouse immortalized fibroblasts transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  ........This gRNA targets the Ai9 and related transgenes ..AAV shuttle vector with CMV promoter driven ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene experimentName ->  Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus
AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA
View Associated: 
AAV-CMV-SaCas9-U6-modified scaffold-Sa-L3   - Vector
Matched on: Name ->  Ai9 mouse immortalized fibroblasts modelName ->  Ai9 mouse immortalized fibroblasts transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  ....This gRNA targets the Ai9 and related transgenes ..AAV shuttle vector with CMV promoter driven SaCas9 ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene experimentName ->  Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus
AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA with modified scaffold sequence
View Associated: 
AAV-CMV-SaCas9-U6-modified scaffold-Sa-LoxP2   - Vector
Matched on: Name ->  Ai9 mouse immortalized fibroblasts modelName ->  Ai9 mouse immortalized fibroblasts transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  ..This gRNA targets the mTmG, Ai9 and related transgenes at two sites..AAV shuttle vector with CMV promoter ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus ->  Ai9/Ai14/mTmG and related reporter transgene (within loxP) guideTargetLocus ->  Ai9/Ai14/mTmG and related reporter transgene (within loxP) experimentName ->  Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus
AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA with modified scaffold sequence
View Associated: 
AAV9-CMV-SaCas9   - Vector
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  .......................AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 ; AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 ; SaCas9..AAVcc47 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
AAV serotype 9 delivering CMV driven SaCas9
View Associated: 
BI28:AAV-GFAP-SaCas9-WPRE3-pA   - Vector
Matched on: Name ->  Ai9 mouse modelName ->  Ai9 mouse transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  ................................This gRNA targets the Ai9 and related transgenes ..Novel engineered AAV ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene experimentName ->  Testing gRNA sequence and gRNA scaffold modified in Ai9 mice.
Novel engineered AAV BI28 variant expressing S. aureus Cas9 driven by the glial fibrillary acidic protein (GFAP) promoter
View Associated: 
pAAV.pU1a-SpCas9   - Vector
Matched on: Name ->  HEK-293T with Ai9 transient reporter assay modelName ->  HEK-293T with Ai9 transient reporter assay transgene ->  Ai9 plasmid generatedDescription ->  HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 and related transgenes ..AAV2/5 expressing SpyCas9 ; HA-SV40NLS-SpCas9-SV40NLS..Lipid nanoparticle..HEK-293T cells transfected with an Ai9 inducible transgene Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Expresses codon-optimized SpCas9 in mammalian cells. HA-SV40NLS-SpCas9-SV40NLS
View Associated: 
Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea   - Experiment - [In Vitro]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple ; It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Chen Zheng-Yi
LNP delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea (inner ear)
View Associated: 
Alt-R® S.p. Cas9 Nuclease V3   - Genome Editor
Matched on: generatedDescription ->  ....This sgRNA targets the Ai9 and related transgenes ....Shuttle peptide used to deliver reagents to Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
View Associated: 
Ai14 gRNA   - Guide - [In Vivo]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) description ->  This sgRNA targets the Ai9 and related transgenes at multiple sites generatedDescription ->  This sgRNA targets the Ai9 and related transgenes at multiple sites....The nanocapsule is a thin glutathione Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
This sgRNA targets the Ai9 and related transgenes at multiple sites
View Associated: 
Ai14 mouse primary fibroblasts   - Model System - [In Vitro]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Primary fibroblasts from Ai14 mice
View Associated: 
AB_2286684    - Antibody - [In Vivo]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Sox-2 (Y-17) Antibody, Santa Cruz Biotechnology
View Associated: 
SaLoxP1-modified   - Guide
Matched on: description ->  This gRNA targets the mTmG, Ai9 and related transgenes at two sites
This gRNA targets the mTmG, Ai9 and related transgenes at two sites
SaLoxP2-unmodified   - Guide
Matched on: description ->  This gRNA targets the mTmG, Ai9 and related transgenes at two sites
This gRNA targets the mTmG, Ai9 and related transgenes at two sites
AAV-CMV-SaCas9-U6-gRNA-LoxP-Sa-L1   - Vector
Matched on: Name ->  Ai9 mouse immortalized fibroblasts modelName ->  Ai9 mouse immortalized fibroblasts transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  ......This gRNA targets the Ai9 and related transgenes ..AAV shuttle vector with CMV promoter driven ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene experimentName ->  Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus
AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA
View Associated: 
AAV-CMV-SaCas9-U6-gRNA-LoxP-Sa-LoxP1   - Vector
Matched on: Name ->  Ai9 mouse immortalized fibroblasts modelName ->  Ai9 mouse immortalized fibroblasts transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  ..This gRNA targets the mTmG, Ai9 and related transgenes at two sites..AAV shuttle vector with CMV promoter ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus ->  Ai9/Ai14/mTmG and related reporter transgene (within loxP) guideTargetLocus ->  Ai9/Ai14/mTmG and related reporter transgene (within loxP) experimentName ->  Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus
AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA
View Associated: 
AAV-CMV-SaCas9-U6-gRNA-LoxP-Sa-R1   - Vector
Matched on: Name ->  Ai9 mouse immortalized fibroblasts modelName ->  Ai9 mouse immortalized fibroblasts transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  ........This gRNA targets the Ai9 and related transgenes ..AAV shuttle vector with CMV promoter driven ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene experimentName ->  Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus
AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA
View Associated: 
AAV-CMV-SaCas9-U6-modified scaffold-Sa-LoxP1   - Vector
Matched on: Name ->  Ai9 mouse immortalized fibroblasts modelName ->  Ai9 mouse immortalized fibroblasts transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  ..This gRNA targets the mTmG, Ai9 and related transgenes at two sites..AAV shuttle vector with CMV promoter ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus ->  Ai9/Ai14/mTmG and related reporter transgene (within loxP) guideTargetLocus ->  Ai9/Ai14/mTmG and related reporter transgene (within loxP) experimentName ->  Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus
AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA with modified scaffold sequence
View Associated: 
AAV-CMV-SaCas9-U6-modified scaffold-Sa-R1   - Vector
Matched on: Name ->  Ai9 mouse immortalized fibroblasts modelName ->  Ai9 mouse immortalized fibroblasts transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  ........This gRNA targets the Ai9 and related transgenes ..AAV shuttle vector with CMV promoter driven ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene experimentName ->  Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus
AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA with modified scaffold sequence
View Associated: 
Lipofectamine 3000   - Delivery System - [In Vitro]
Matched on: Name ->  HEK-293T with Ai9 transient reporter assay modelName ->  HEK-293T with Ai9 transient reporter assay transgene ->  Ai9 plasmid generatedDescription ->  targeting eGFP compatible with CasΦ-2..Guide targeting eGFP compatible with CasΦ-1..This sgRNA targets the Ai9 ; -1 and spacer for editing in human cells. ..Lipid nanoparticle..HEK-293T cells transfected with an Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Lipid nanoparticle
View Associated: 
Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia   - Experiment - [In Vivo]
Matched on: generatedDescription ->  of GFP protein by GFP positive cells compared to DAPI stained cells...........This sgRNA targets the Ai9 ; and related transgenes ..This sgRNA targets the Ai9 and related transgenes at two sites....Shuttle peptide ; EGFP (mG) fluorescence expression replacing the red fluorescence............This sgRNA targets the Ai9 ; and related transgenes ..This sgRNA targets the Ai9 and related transgenes at two sites....Shuttle peptide Guide Target Locus ->  Ai9/Ai14 reporter transgene (within loxP) ; Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene (within loxP) ; Ai9/Ai14 reporter transgene
McCray Paul B
In vivo shuttle peptide delivery of Cas9 and Cas12a RNPs in mouse airway epithelia. Gene editing was quantified by the GFP+ cells in large and small airways following 1 delivery of GFP protein by GFP positive cells compared to DAPI stained cells.
View Associated: 
Cre recombinase   - Genome Editor
Matched on: Name ->  HEK-293T with Ai9 transient reporter assay modelName ->  HEK-293T with Ai9 transient reporter assay transgene ->  Ai9 plasmid generatedDescription ->  recombinase delivered by scAAV5-Cre-GFP plasmid......Lipid nanoparticle..HEK-293T cells transfected with an Ai9
Cre recombinase delivered by scAAV5-Cre-GFP plasmid
View Associated: 
Cre recombinase   - Genome Editor
Matched on: Name ->  Ai9 mouse immortalized fibroblasts modelName ->  Ai9 mouse immortalized fibroblasts transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 experimentName ->  Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus
Cre recombinase delivered by pAAV-EF1a-Cre plasmid
View Associated: 
AAV-CMV-SaCas9-U6-gRNA-LoxP-Sa-L2   - Vector
Matched on: Name ->  Ai9 mouse immortalized fibroblasts modelName ->  Ai9 mouse immortalized fibroblasts transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  ......This gRNA targets the Ai9 and related transgenes ..AAV shuttle vector with CMV promoter driven ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene experimentName ->  Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus
AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA
View Associated: 
AAV-CMV-SaCas9-U6-gRNA-LoxP-Sa-LoxP2   - Vector
Matched on: Name ->  Ai9 mouse immortalized fibroblasts modelName ->  Ai9 mouse immortalized fibroblasts transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  ..This gRNA targets the mTmG, Ai9 and related transgenes at two sites..AAV shuttle vector with CMV promoter ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus ->  Ai9/Ai14/mTmG and related reporter transgene (within loxP) guideTargetLocus ->  Ai9/Ai14/mTmG and related reporter transgene (within loxP) experimentName ->  Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus
AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA
View Associated: 
AAV-CMV-SaCas9-U6-gRNA-LoxP-Sa-R2   - Vector
Matched on: Name ->  Ai9 mouse immortalized fibroblasts modelName ->  Ai9 mouse immortalized fibroblasts transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  ........This gRNA targets the Ai9 and related transgenes ..AAV shuttle vector with CMV promoter driven ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene experimentName ->  Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus
AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA
View Associated: 
AAV-CMV-SaCas9-U6-modified scaffold-Sa-L1   - Vector
Matched on: Name ->  Ai9 mouse immortalized fibroblasts modelName ->  Ai9 mouse immortalized fibroblasts transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  ......This gRNA targets the Ai9 and related transgenes ..AAV shuttle vector with CMV promoter driven ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene experimentName ->  Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus
AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA with modified scaffold sequence
View Associated: 
SaLoxP1-unmodified   - Guide
Matched on: description ->  This gRNA targets the mTmG, Ai9 and related transgenes at two sites
This gRNA targets the mTmG, Ai9 and related transgenes at two sites
CleanCap® Cas9 mRNA (5moU)   - Genome Editor
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
SpCas9 mRNA with 2 NLS signals, HA tag and capped using CleanCap. It is polyadenylated, substituted with a modified uridine and optimized for mammalian systems. It mimics a fully processed mature mRNA.
View Associated: 
sNLS-SpCas9-sNLS   - Genome Editor
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
SpCas9 with N- and C-terminal SV40 NLS
View Associated: 
Ai14 mouse   - Model System - [In Vivo]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain.
View Associated: 
g-loxPbot_C12a   - Guide - [In Vivo]
Matched on: description ->  This sgRNA targets the Ai9 and related transgenes at two sites generatedDescription ->  This sgRNA targets the Ai9 and related transgenes at two sites....Shuttle peptide used to deliver reagents Guide Target Locus ->  Ai9/Ai14 reporter transgene (within loxP) guideTargetLocus ->  Ai9/Ai14 reporter transgene (within loxP)
This sgRNA targets the Ai9 and related transgenes at two sites
View Associated: 
sg298   - Guide - [In Vivo, In Vitro]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) description ->  This sgRNA targets the Ai9 and related transgenes at multiple sites generatedDescription ->  This sgRNA targets the Ai9 and related transgenes at multiple sites....combinatorial library cationic Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
This sgRNA targets the Ai9 and related transgenes at multiple sites
View Associated: 
SaLoxP2-modified   - Guide
Matched on: description ->  This gRNA targets the mTmG, Ai9 and related transgenes at two sites
This gRNA targets the mTmG, Ai9 and related transgenes at two sites
SpCas9   - Genome Editor
Matched on: Name ->  Ai9 mouse (BCM) modelName ->  Ai9 mouse (BCM) transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  two sites, or Cre recombinase between loxP sites, tdTomato expression is lost and GFP is expressed...Ai9
View Associated: 
pH509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1)   - Vector
Matched on: Name ->  HEK-293T with Ai9 transient reporter assay modelName ->  HEK-293T with Ai9 transient reporter assay transgene ->  Ai9 plasmid generatedDescription ->  ..HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 and related transgenes ..AAV2/5 expressing SpyCas9 ; HA-SV40NLS-SpCas9-SV40NLS..Lipid nanoparticle..HEK-293T cells transfected with an Ai9 inducible transgene Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
AAV2/5 expressing SpyCas9. AAV2/5 expressing two sgRNAs under U6 promoter and eGFP
View Associated: 
mTmG mouse   - Model System - [In Vivo]
Matched on: generatedDescription ->  ........This sgRNA targets the Ai9 and related transgenes ..This sgRNA targets the Ai9 and related transgenes Guide Target Locus ->  Ai9/Ai14 reporter transgene (within loxP) ; Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene (within loxP) ; Ai9/Ai14 reporter transgene
ROSAmT/mG is a cell membrane-targeted, two-color fluorescent Cre-reporter allele. Prior to Cre recombination, cell membrane-localized tdTomato (mT) fluorescence expression is widespread in cells/tissues. Cre recombinase expressing cells (and future cell lineages derived from these cells) have cell membrane-localized EGFP (mG) fluorescence expression replacing the red fluorescence
View Associated: 
AsCas12a (IDT and Feldan Therapeutics)   - Genome Editor
Matched on: generatedDescription ->  ....This sgRNA targets the Ai9 and related transgenes at two sites....Shuttle peptide used to deliver Guide Target Locus ->  Ai9/Ai14 reporter transgene (within loxP) guideTargetLocus ->  Ai9/Ai14 reporter transgene (within loxP)
View Associated: 
Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea   - Experiment - [In Vivo]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple ; It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Chen Zheng-Yi
Delivery of Cas9/sgRNA mRNA via new LNPs to the cochlea by cochleostomy and gene editing is measured by percentage of tdTomato positive cells.
View Associated: 
CleanCap® Cas9   - Genome Editor
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
SpCas9 mRNA with 2 NLS signals, HA tag and capped using CleanCap. It is polyadenylated, substituted with a modified uridine and optimized for mammalian systems. It mimics a fully processed mature mRNA.
View Associated: 
306-O12B   - Delivery System - [In Vivo, In Vitro]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple ; sites..This sgRNA targets the Ai9 and related transgenes at multiple sites. 2'-O-Methyl at 3 first Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Combinatorial library cationic lipid nanoparticles
View Associated: 
RNP-NC-CPP   - Delivery System - [In Vivo]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a cell penetrating peptide (CPP) from the TAT peptide (GRKKRRQRRRPQ) which lacks cell-type specficity
View Associated: 
AB_10015251    - Antibody - [In Vivo]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Anti-Myosin VIIa antibody, Proteus Biosciences
View Associated: 
[] Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain   - Experiment - [In Vivo]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 ; SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Murray Stephen A
Delivery of CRISPR/Cas9 via RNP-loaded nanocages to the brain in Ai14 mice
View Associated: 
113-O12B   - Delivery System - [In Vivo]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
combinatorial library cationic lipid nanoparticles
View Associated: 
Ai14 mouse (congenic)   - Model System - [In Vivo]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  mimics a fully processed mature mRNA...SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 ; and related transgenes at multiple sites..This sgRNA targets the Ai9 and related transgenes at multiple Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain.
View Associated: 
AB_2532994    - Antibody - [In Vivo]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Thermo-Fisher, Rat anti-GFAP
View Associated: 
AB_141637    - Antibody - [In Vivo]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Invitrogen, Donkey anti-rabbit alexa fluor 594
View Associated: 
AB_141607    - Antibody - [In Vivo]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Invitrogen, Donkey anti-mouse alexafluor 488
View Associated: 
Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.   - Experiment - [In Vivo]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 ; SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Gong Shaoqin (Sarah)
Nanocapusules carrying CRISPR Cas9 RNP with guide RNA targeting the stop sequence in the Ai14 transgene are intracerebrally delivered to Ai14 mice and gene editing is measured by gain of tdTomato protein expression.
View Associated: 
[] Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear   - Experiment - [In Vivo]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple ; It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Murray Stephen A
Delivery of CRISPR/Cas9 via bioreducible lipid nanoparticles (LNPs) to the inner ear in Ai14 mice
View Associated: 
[] Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear   - Experiment - [In Vivo]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple ; It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Murray Stephen A
Delivery of CRISPR/Cas9 editor via bioreducible lipid nanoparticle to the inner ear in Ai14 mice
View Associated: 
306-S10   - Delivery System - [In Vivo, In Vitro]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple ; sites..This sgRNA targets the Ai9 and related transgenes at multiple sites. 2'-O-Methyl at 3 first Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
combinatorial library cationic lipid nanoparticles
View Associated: 
RNP-NC-no ligand   - Delivery System - [In Vivo]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA.
View Associated: 
RNP-NC-RVG   - Delivery System - [In Vivo]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a RVG peptide YTIWMPENPRPGTPCDIFTNSRGKRASNG which specifically interacts withthe N-acetylecholine receptor (AchR) on neuronal cells, which mediates NP entry
View Associated: 
sg298   - Guide - [In Vivo]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) description ->  This sgRNA targets the Ai9 and related transgenes at multiple sites. 2'-O-Methyl at 3 first and last generatedDescription ->  This sgRNA targets the Ai9 and related transgenes at multiple sites. 2'-O-Methyl at 3 first and last Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
This sgRNA targets the Ai9 and related transgenes at multiple sites. 2'-O-Methyl at 3 first and last bases, 3' phosphorothioate bonds between first 3 and last 2 bases
View Associated: 
AB_10711040    - Antibody - [In Vivo]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Abcam, mouse anti-NeuN
View Associated: 
AB_2813835    - Antibody - [In Vivo]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription ->  SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene
Abcam, Donkey anti-rat alexa fluour 647
View Associated: 
S10   - Delivery System - [In Vivo, In Vitro]
Matched on: generatedDescription ->  C-Myc-like NLS at the N-terminal SV40 NLS and Nucleoplasmin NLS at the C-terminal..This sgRNA targets the Ai9 ; transgenes..gRNA targeting human NKG2A exon 3..gRNA targeting CFTR inton-22-23..This sgRNA targets the Ai9 Guide Target Locus ->  Ai9/Ai14 reporter transgene (within loxP) ; Ai9/Ai14 reporter transgene guideTargetLocus ->  Ai9/Ai14 reporter transgene (within loxP) ; Ai9/Ai14 reporter transgene
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
306-O12B blank   - Delivery System - [In Vivo]
Matched on: transgene ->  Ai9 (CAG-lox-stop-lox-tdTomato)
Combinatorial library cationic lipid nanoparticles
View Associated: 

115 results for ai9

Category Type Name Description View Associated..
Model System Animal Ai9 mouse Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus.
Model System Animal Ai9 mouse (BCM) Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus.
Model System Cell Ai9 mouse immortalized fibroblasts Immortalized fibroblasts made from Ai9 (B6.Cg-Gt(ROSA)26Sor^tm9(CAG-tdTomato)Hze/J) mice
Guide Ai9 SaCas9 Guide B This gRNA targets the Ai9 and related transgenes
Guide Ai9 SaCas9 Guide A This gRNA targets the Ai9 and related transgenes
Model System Cell HEK-293T with Ai9 transient reporter assay HEK-293T cells transfected with an Ai9 inducible transgene reporter plasmid used to test gene editing activity by fluorescence
Experiment In Vivo Cre Recombinase dose escalation study in Ai9 mice A single stranded cmv cre cassette was packaged into AAV9 or AAVcc47 and injected intravenously in Ai9 mice. We injected n=3 at three different doses (1e10, 1e11, 1e12 vg) and harvested organs 4 weeks post injection. Fluorescence intensity in liver, heart, and skeletal muscle was quantified with tiff based images in Image J and neuronal transduction from each vector was quantified at the 1e12vg dose by counting the number of tdTomato+ neurons and number of NeuN+ cells from multiple sections and images.
Experiment In Vivo Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. 3e11 vg/mouse of AAV-BI28:GFAP-SaCas9-WPRE-pA and 3e11 vg/mouse of AAV-BI28:GFAP-NLS-GFP-U6-L1-U6-R2 were codelivered intravenously to adult male and female Ai9 mice. Editing was assessed in brain sections 4 weeks later.
Experiment In Vitro Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus in vitro Reporter transgene activation by SaCas9 gRNA target and modified scaffold sequences by transient transfection in immortalized Ai9 mouse fibroblasts
Experiment In Vivo Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) A dual vector strategy was employed: one delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (both single stranded AAV cassettes). This strategy was evaluted with both AAV9 (n=3) and AAVcc47 (n=3) by intravenous injection in Ai9 mice. A total dose of 3e12vg was injected into each mouse (1.5e12vg each vector mixed 1:1) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
Experiment In Vivo Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter) A dual vector strategy was employed: one delivering a single guide RNA and CB driven SaCas9, and another delivering the second guide RNA and CB driven SaCas9. This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice. A total dose of 2e12vg was injected into each mouse (1e12vg each vector mixed 1:1) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
Experiment In Vivo Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter) A dual vector strategy was employed: one delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (both single stranded AAV cassettes). This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice. A total dose of 4e12vg was injected into each mouse and vectors mixed in a 1:4 ratio of cas9 to guide RNA (1e12vg of CMV Sacas9 vector and 3e12vg of the sgRNA vector) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
Experiment In Vivo Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector. A dual vector strategy was employed: one self complementary vector delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (single stranded vector). This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=4) by intravenous injection in Ai9 mice. A total dose of 4e12vg was injected into each mouse and vectors mixed in a 1:1 ratio of cas9 to guide RNA (2e12vg of CMV Sacas9 vector and 2e12vg of the self complementary sgRNA vector) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
Experiment In Vivo Validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing. Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery. Detection of editing in non-target tissues.
Genome Editor Cas Nuclease SauCas9
Vector Viral Vector AAVcc47-SaCas9-Ai9 AAV2/9 expressing SaCas9 and single sgRNA under U6 promoter
Genome Editor Cas Nuclease SaCas9
Vector Viral Vector AAVcc47-CMV-Cre AAVcc47 delivering CMV Cre Recombinase
Experiment In Vivo Testing AAV5 for activation of tdTomato in mouse airway AAV2/5 mediated gene editing in the mouse airway was tested by deliverying SpCas9 and guide RNAs targeting the Ai9 transgene in Ai9 transgenic mice. Viral delivery was detected by GFP expression and gene editing quantified by tdTomato activation
Experiment In Vivo Second site validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain Validation of delivery of AAV custom designed to cross the blood-brain barrier for CRISPR/Cas9 editing. Editing detected and quantified in brain by generation of tdTomato fluorescent protein signal from Ai9 reporter mice
Vector Viral Vector AAV9-Ai9-sgRNA1-CB-SaCas9 AAV serotype 9 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus
Vector Viral Vector AAV9-Ai9-sgRNA2-CB-SaCas9 AAV serotype 9 delivering gRNA 2 + CB SaCas9 targeting the Ai9 locus
Vector Viral Vector AAVcc47-Ai9-sgRNA1-CB-SaCas9 AAVcc47 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus