25 results for ai9

Ai14 gRNA   - Guide [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo]
Matched on: name -->  Ai14 gRNA  -->  Ai14 gRNA
This sgRNA targets the Ai9 and related transgenes at multiple sites
Ai9 mouse (BCM)   - Model System [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on: name -->  Ai9 mouse (BCM)  -->  Ai9 SaCas9 Guide A  Ai9 SaCas9 Guide B
Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus.
Ai14 mouse   - Model System [Delivery Systems Initiative] - [In Vivo]
Matched on: name -->  Ai14 mouse  -->  Ai14 gRNA
Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain.
Ai9 SaCas9 Guide A   - Guide [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on: name -->  Ai9 SaCas9 Guide A  -->  Ai9 SaCas9 Guide A  Ai9 SaCas9 Guide B
This gRNA targets the Ai9 and related transgenes
Ai14 mouse primary fibroblasts   - Model System [Delivery Systems Initiative] - [In Vitro]
Matched on: name -->  Ai14 mouse primary fibroblasts
Primary fibroblasts from Ai14 mice
Ai14 mouse (congenic)   - Model System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo]
Matched on: name -->  Ai14 mouse (congenic)  -->  Ai14 gRNA
Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain.
Ai9 mouse   - Model System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo]
Matched on: name -->  Ai9 mouse
Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus.
Ai9 SaCas9 Guide B   - Guide [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on: name -->  Ai9 SaCas9 Guide B  -->  Ai9 SaCas9 Guide A  Ai9 SaCas9 Guide B
This gRNA targets the Ai9 and related transgenes
Ai9 mouse immortalized fibroblasts   - Model System [Delivery Systems Initiative] - [In Vitro]
Matched on: name -->  Ai9 mouse immortalized fibroblasts
Immortalized fibroblasts made from Ai9 (B6.Cg-Gt(ROSA)26Sor^tm9(CAG-tdTomato)Hze/J) mice
Ai9-SauSpyCas9 mouse   - Model System [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on: name -->  Ai9-SauSpyCas9 mouse
Ai9-SauSpyCas9 is a version of Ai9 that has a single guide RNA sites on both sides of the STOP cassette. This is a Cre reporter allele that has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. Ai9 mice express robust tdTomato fluorescence following Cre-mediated recombination.
AB_2813835    - Antibody [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Ai14 gRNA
Other Id: ab150155 
Abcam, Donkey anti-rat alexa fluour 647
[Validation] Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain   - Experiment [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Ai14 gRNA
Murray Stephen A
Delivery of CRISPR/Cas9 via RNP-loaded nanocages to the brain in Ai14 mice
AB_2532994    - Antibody [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Ai14 gRNA
Other Id: 13-0300  
Thermo-Fisher, Rat anti-GFAP
AB_141637    - Antibody [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Ai14 gRNA
Other Id: A21207 
Invitrogen, Donkey anti-rabbit alexa fluor 594
Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.   - Experiment [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Ai14 gRNA
Gong Shaoqin (Sarah)
Nanocapusules carrying CRISPR Cas9 RNP with guide RNA targeting the stop sequence in the Ai14 transgene are intracerebrally delivered to Ai14 mice and gene editing is measured by gain of tdTomato protein expression.
AB_2209751    - Antibody [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Ai14 gRNA
Other Id: Rockland Cat# 600-401-379 
Anti-RFP (RABBIT) Antibody
AB_10711040    - Antibody [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Ai14 gRNA
Other Id: ab104224  
Abcam, mouse anti-NeuN
RNP-NC-no ligand   - Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Ai14 gRNA
The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA.
sNLS-SpCas9-sNLS   - Genome Editor  [Small Animal Testing Center (SATC), Delivery Systems Initiative]
Matched on:  -->  Ai14 gRNA
SpCas9 with N- and C-terminal SV40 NLS
RNP-NC-CPP   - Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Ai14 gRNA
The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a cell penetrating peptide (CPP) from the TAT peptide (GRKKRRQRRRPQ) which lacks cell-type specficity
RNP-NC-RVG   - Delivery System [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Ai14 gRNA
The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a RVG peptide YTIWMPENPRPGTPCDIFTNSRGKRASNG which specifically interacts withthe N-acetylecholine receptor (AchR) on neuronal cells, which mediates NP entry
AB_141607    - Antibody [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Ai14 gRNA
Other Id: A21202 
Invitrogen, Donkey anti-mouse alexafluor 488
AAVcc47-SaCas9-Ai9   - Vector  [Small Animal Testing Center (SATC)]
Matched on:  -->  Ai9 SaCas9 Guide A  Ai9 SaCas9 Guide B
AAV2/9 expressing SaCas9 and single sgRNA under U6 promoter
[Validation] Validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing.   - Experiment [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Ai9 SaCas9 Guide A  Ai9 SaCas9 Guide B
Heaney Jason D
Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery. Detection of editing in non-target tissues.
SauCas9   - Genome Editor  [Small Animal Testing Center (SATC)]
Matched on:  -->  Ai9 SaCas9 Guide A  Ai9 SaCas9 Guide B

25 results for ai9

Category Type Name Description View Associated..
Guide Ai14 gRNA This sgRNA targets the Ai9 and related transgenes at multiple sites
Model System Animal Ai9 mouse (BCM) Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus.
Model System Animal Ai14 mouse Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain.
Guide Ai9 SaCas9 Guide A This gRNA targets the Ai9 and related transgenes
Model System Cell Ai14 mouse primary fibroblasts Primary fibroblasts from Ai14 mice
Model System Animal Ai14 mouse (congenic) Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain.
Model System Animal Ai9 mouse Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus.
Guide Ai9 SaCas9 Guide B This gRNA targets the Ai9 and related transgenes
Model System Cell Ai9 mouse immortalized fibroblasts Immortalized fibroblasts made from Ai9 (B6.Cg-Gt(ROSA)26Sor^tm9(CAG-tdTomato)Hze/J) mice
Model System Animal Ai9-SauSpyCas9 mouse Ai9-SauSpyCas9 is a version of Ai9 that has a single guide RNA sites on both sides of the STOP cassette. This is a Cre reporter allele that has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. Ai9 mice express robust tdTomato fluorescence following Cre-mediated recombination.
Antibody AB_2813835  Abcam, Donkey anti-rat alexa fluour 647
Experiment In Vivo Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Delivery of CRISPR/Cas9 via RNP-loaded nanocages to the brain in Ai14 mice
Antibody AB_2532994  Thermo-Fisher, Rat anti-GFAP
Antibody AB_141637  Invitrogen, Donkey anti-rabbit alexa fluor 594
Experiment In Vivo Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. Nanocapusules carrying CRISPR Cas9 RNP with guide RNA targeting the stop sequence in the Ai14 transgene are intracerebrally delivered to Ai14 mice and gene editing is measured by gain of tdTomato protein expression.
Antibody AB_2209751  Anti-RFP (RABBIT) Antibody
Antibody AB_10711040  Abcam, mouse anti-NeuN
Delivery System Nanoparticle RNP-NC-no ligand The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA.
Genome Editor Cas Nuclease sNLS-SpCas9-sNLS SpCas9 with N- and C-terminal SV40 NLS
Delivery System Nanoparticle RNP-NC-CPP The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a cell penetrating peptide (CPP) from the TAT peptide (GRKKRRQRRRPQ) which lacks cell-type specficity
Delivery System Nanoparticle RNP-NC-RVG The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a RVG peptide YTIWMPENPRPGTPCDIFTNSRGKRASNG which specifically interacts withthe N-acetylecholine receptor (AchR) on neuronal cells, which mediates NP entry
Antibody AB_141607  Invitrogen, Donkey anti-mouse alexafluor 488
Vector Viral Vector AAVcc47-SaCas9-Ai9 AAV2/9 expressing SaCas9 and single sgRNA under U6 promoter
Experiment In Vivo Validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing. Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery. Detection of editing in non-target tissues.
Genome Editor Cas Nuclease SauCas9