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Ai9 mouse - Model System - [In Vivo] Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) description -> Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent generatedDescription -> ..................................................HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 ; and related transgenes ..This gRNA targets the Ai9 and related transgenes ..AAV serotype 9 delivering ; u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus..AAVcc47 delivering sgRNA 2 + CB SaCas9 ; HA-SV40NLS-SpCas9-SV40NLS..AAV serotype 9 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus..AAV ; serotype 9 delivering gRNA 2 + CB SaCas9 targeting the Ai9 locus..Novel engineered AAV BI28 variant Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene experimentName -> Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Cre Recombinase dose escalation study in Ai9 mice Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. View Associated: Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases Novel AAVs Engineered for Efficient and Noninvasive Cross-Species Gene Editing Throughout the Central Nervous System Evolving High Potency AAV Vectors for Neuromuscular Genome Editing BCM-Rice Resource for the Analysis of Somatic Gene Editing in Mice - Deverman Validation (Deverman, Benjamin) Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter) Testing AAV5 for activation of tdTomato in mouse airway Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) Cre Recombinase dose escalation study in Ai9 mice Second site validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.
Ai9 mouse (BCM) - Model System - [In Vivo] Matched on: Name -> Ai9 mouse (BCM) modelName -> Ai9 mouse (BCM) transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) grnaLabId -> Ai9 SaCas9 Guide A ; Ai9 SaCas9 Guide B description -> Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent generatedDescription -> AAVcc47-Cre viral vector..Cre recombinase delivered by AAVcc47-Cre viral vector..This sgRNA targets the Ai9 ; and related transgenes ..This gRNA targets the Ai9 and related transgenes ..AAV2/9 expressing SaCas9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. View Associated:
Ai9 mouse immortalized fibroblasts - Model System - [In Vitro] Matched on: Name -> Ai9 mouse immortalized fibroblasts modelName -> Ai9 mouse immortalized fibroblasts transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) description -> Immortalized fibroblasts made from Ai9 (B6.Cg-Gt(ROSA)26Sor^tm9(CAG-tdTomato)Hze/J) mice generatedDescription -> pAAV-EF1a-Cre plasmid..Cre recombinase delivered by pAAV-EF1a-Cre plasmid..This gRNA targets the mTmG, Ai9 ; and related transgenes at two sites..This gRNA targets the Ai9 and related transgenes ..AAV shuttle ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene ; Ai9/Ai14/mTmG and related reporter transgene (within loxP) guideTargetLocus -> Ai9/Ai14 reporter transgene ; Ai9/Ai14/mTmG and related reporter transgene (within loxP) experimentName -> Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus Immortalized fibroblasts made from Ai9 (B6.Cg-Gt(ROSA)26Sor^tm9(CAG-tdTomato)Hze/J) mice View Associated:
Ai9 SaCas9 Guide B - Guide Matched on: Name -> Ai9 SaCas9 Guide B description -> This gRNA targets the Ai9 and related transgenes This gRNA targets the Ai9 and related transgenes
Ai9 SaCas9 Guide A - Guide Matched on: Name -> Ai9 SaCas9 Guide A description -> This gRNA targets the Ai9 and related transgenes This gRNA targets the Ai9 and related transgenes
HEK-293T with Ai9 transient reporter assay - Model System - [In Vitro] Matched on: Name -> HEK-293T with Ai9 transient reporter assay modelName -> HEK-293T with Ai9 transient reporter assay transgene -> Ai9 plasmid description -> HEK-293T cells transfected with an Ai9 inducible transgene reporter plasmid used to test gene editing generatedDescription -> recombinase delivered by scAAV5-Cre-GFP plasmid..HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 ; HA-SV40NLS-SpCas9-SV40NLS..Lipid nanoparticle..HEK-293T cells transfected with an Ai9 inducible transgene Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene HEK-293T cells transfected with an Ai9 inducible transgene reporter plasmid used to test gene editing activity by fluorescence View Associated:
Cre Recombinase dose escalation study in Ai9 mice - Experiment - [In Vivo] Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) description -> A single stranded cmv cre cassette was packaged into AAV9 or AAVcc47 and injected intravenously in Ai9 generatedDescription -> A single stranded cmv cre cassette was packaged into AAV9 or AAVcc47 and injected intravenously in Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName -> Cre Recombinase dose escalation study in Ai9 mice Asokan Aravind A single stranded cmv cre cassette was packaged into AAV9 or AAVcc47 and injected intravenously in Ai9 mice. We injected n=3 at three different doses (1e10, 1e11, 1e12 vg) and harvested organs 4 weeks post injection. Fluorescence intensity in liver, heart, and skeletal muscle was quantified with tiff based images in Image J and neuronal transduction from each vector was quantified at the 1e12vg dose by counting the number of tdTomato+ neurons and number of NeuN+ cells from multiple sections and images. View Associated:
Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. - Experiment - [In Vivo] Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) description -> /mouse of AAV-BI28:GFAP-NLS-GFP-U6-L1-U6-R2 were codelivered intravenously to adult male and female Ai9 generatedDescription -> /mouse of AAV-BI28:GFAP-NLS-GFP-U6-L1-U6-R2 were codelivered intravenously to adult male and female Ai9 ; Editing was assessed in brain sections 4 weeks later. ..............This gRNA targets the Ai9 and related ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 ; variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus.................This gRNA targets the Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene experimentName -> Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. Deverman Benjamin E 3e11 vg/mouse of AAV-BI28:GFAP-SaCas9-WPRE-pA and 3e11 vg/mouse of AAV-BI28:GFAP-NLS-GFP-U6-L1-U6-R2 were codelivered intravenously to adult male and female Ai9 mice. Editing was assessed in brain sections 4 weeks later. View Associated:
Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus in vitro - Experiment - [In Vitro] Matched on: Name -> Ai9 mouse immortalized fibroblasts modelName -> Ai9 mouse immortalized fibroblasts transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) description -> activation by SaCas9 gRNA target and modified scaffold sequences by transient transfection in immortalized Ai9 generatedDescription -> activation by SaCas9 gRNA target and modified scaffold sequences by transient transfection in immortalized Ai9 ; pAAV-EF1a-Cre plasmid..Cre recombinase delivered by pAAV-EF1a-Cre plasmid..This gRNA targets the mTmG, Ai9 ; and related transgenes at two sites..This gRNA targets the Ai9 and related transgenes ..AAV shuttle ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 ; and related transgenes at two sites..This gRNA targets the Ai9 and related transgenes ..AAV shuttle Guide Target Locus -> Ai9/Ai14 reporter transgene ; Ai9/Ai14/mTmG and related reporter transgene (within loxP) guideTargetLocus -> Ai9/Ai14 reporter transgene ; Ai9/Ai14/mTmG and related reporter transgene (within loxP) experimentName -> Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus Deverman Benjamin E Reporter transgene activation by SaCas9 gRNA target and modified scaffold sequences by transient transfection in immortalized Ai9 mouse fibroblasts View Associated:
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) - Experiment - [In Vivo] Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) description -> This strategy was evaluted with both AAV9 (n=3) and AAVcc47 (n=3) by intravenous injection in Ai9 mice generatedDescription -> This strategy was evaluted with both AAV9 (n=3) and AAVcc47 (n=3) by intravenous injection in Ai9 mice ; . ....................AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 ; .......................AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to Asokan Aravind A dual vector strategy was employed: one delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (both single stranded AAV cassettes). This strategy was evaluted with both AAV9 (n=3) and AAVcc47 (n=3) by intravenous injection in Ai9 mice. A total dose of 3e12vg was injected into each mouse (1.5e12vg each vector mixed 1:1) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart. View Associated:
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter) - Experiment - [In Vivo] Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) description -> This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice generatedDescription -> This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice ; locus..AAV serotype 9 delivering gRNA 2 + CB SaCas9 targeting the Ai9 locus..AAVcc47 delivering sgRNA ; 1 + CB SaCas9 targeting the Ai9 locus..AAV serotype 9 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 ; locus..AAV serotype 9 delivering gRNA 2 + CB SaCas9 targeting the Ai9 locus..AAVcc47 delivering sgRNA ; 1 + CB SaCas9 targeting the Ai9 locus..AAV serotype 9 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to Asokan Aravind A dual vector strategy was employed: one delivering a single guide RNA and CB driven SaCas9, and another delivering the second guide RNA and CB driven SaCas9. This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice. A total dose of 2e12vg was injected into each mouse (1e12vg each vector mixed 1:1) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart. View Associated:
Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter) - Experiment - [In Vivo] Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) description -> This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice generatedDescription -> This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice ; .......................AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 ; .......................AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName -> Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 Asokan Aravind A dual vector strategy was employed: one delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (both single stranded AAV cassettes). This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice. A total dose of 4e12vg was injected into each mouse and vectors mixed in a 1:4 ratio of cas9 to guide RNA (1e12vg of CMV Sacas9 vector and 3e12vg of the sgRNA vector) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart. View Associated:
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector. - Experiment - [In Vivo] Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) description -> This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=4) by intravenous injection in Ai9 mice generatedDescription -> This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=4) by intravenous injection in Ai9 mice ; AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 ; SaCas9..AAVcc47 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 ; AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 ; SaCas9..AAVcc47 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to Asokan Aravind A dual vector strategy was employed: one self complementary vector delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (single stranded vector). This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=4) by intravenous injection in Ai9 mice. A total dose of 4e12vg was injected into each mouse and vectors mixed in a 1:1 ratio of cas9 to guide RNA (2e12vg of CMV Sacas9 vector and 2e12vg of the self complementary sgRNA vector) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart. View Associated:
[] Validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing. - Experiment - [In Vivo] Matched on: Name -> Ai9 mouse (BCM) modelName -> Ai9 mouse (BCM) transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) grnaLabId -> Ai9 SaCas9 Guide A ; Ai9 SaCas9 Guide B generatedDescription -> AAVcc47-Cre viral vector..Cre recombinase delivered by AAVcc47-Cre viral vector..This gRNA targets the Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 ; AAVcc47-Cre viral vector..Cre recombinase delivered by AAVcc47-Cre viral vector..This gRNA targets the Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Heaney Jason D Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery. Detection of editing in non-target tissues. View Associated:
SauCas9 - Genome Editor Matched on: Name -> Ai9 mouse (BCM) modelName -> Ai9 mouse (BCM) transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) grnaLabId -> Ai9 SaCas9 Guide A ; Ai9 SaCas9 Guide B generatedDescription -> ..............................................................................This gRNA targets the Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene View Associated:
AAVcc47-SaCas9-Ai9 - Vector Matched on: Name -> Ai9 mouse (BCM) modelName -> Ai9 mouse (BCM) transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) grnaLabId -> Ai9 SaCas9 Guide A ; Ai9 SaCas9 Guide B generatedDescription -> ..............................................................................This gRNA targets the Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene AAV2/9 expressing SaCas9 and single sgRNA under U6 promoter View Associated:
SaCas9 - Genome Editor Matched on: Name -> Ai9 mouse immortalized fibroblasts ; Ai9 mouse modelName -> Ai9 mouse immortalized fibroblasts ; Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> ........................................................................This gRNA targets the mTmG, Ai9 ; and related transgenes at two sites..This gRNA targets the Ai9 and related transgenes ..AAV shuttle ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 ; variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus...Immortalized fibroblasts made from Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene ; Ai9/Ai14/mTmG and related reporter transgene (within loxP) guideTargetLocus -> Ai9/Ai14 reporter transgene ; Ai9/Ai14/mTmG and related reporter transgene (within loxP) experimentName -> Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. ; Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus View Associated: Novel AAVs Engineered for Efficient and Noninvasive Cross-Species Gene Editing Throughout the Central Nervous System BCM-Rice Resource for the Analysis of Somatic Gene Editing in Mice - Deverman Validation (Deverman, Benjamin) Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus in vitro Second site validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain
AAVcc47-CMV-Cre - Vector Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName -> Cre Recombinase dose escalation study in Ai9 mice AAVcc47 delivering CMV Cre Recombinase View Associated:
Testing AAV5 for activation of tdTomato in mouse airway - Experiment - [In Vivo] Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) description -> mediated gene editing in the mouse airway was tested by deliverying SpCas9 and guide RNAs targeting the Ai9 ; transgene in Ai9 transgenic mice. generatedDescription -> mediated gene editing in the mouse airway was tested by deliverying SpCas9 and guide RNAs targeting the Ai9 ; transgene in Ai9 transgenic mice. ; gene editing quantified by tdTomato activation ..HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 ; RABBIT) Antibody..GFP (D5.1) XP Rabbit mAb antibody..HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Gao Guang-Ping AAV2/5 mediated gene editing in the mouse airway was tested by deliverying SpCas9 and guide RNAs targeting the Ai9 transgene in Ai9 transgenic mice. Viral delivery was detected by GFP expression and gene editing quantified by tdTomato activation View Associated:
[] Second site validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain - Experiment - [In Vivo] Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) description -> Editing detected and quantified in brain by generation of tdTomato fluorescent protein signal from Ai9 generatedDescription -> Editing detected and quantified in brain by generation of tdTomato fluorescent protein signal from Ai9 ; reporter mice..................................This gRNA targets the Ai9 and related transgenes ..Novel ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 ; all inserted into the Gt(ROSA)26Sor locus.....................................This gRNA targets the Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Heaney Jason D Validation of delivery of AAV custom designed to cross the blood-brain barrier for CRISPR/Cas9 editing. Editing detected and quantified in brain by generation of tdTomato fluorescent protein signal from Ai9 reporter mice View Associated:
AAV9-Ai9-sgRNA1-CB-SaCas9 - Vector Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) description -> AAV serotype 9 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus generatedDescription -> ..........AAV serotype 9 delivering gRNA 2 + CB SaCas9 targeting the Ai9 locus..AAV serotype 9 delivering ; sgRNA 1 + CB SaCas9 targeting the Ai9 locus....CAG-loxP-stop-loxP-tdtomato reporter cassette which is ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to AAV serotype 9 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus View Associated:
AAV9-Ai9-sgRNA2-CB-SaCas9 - Vector Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) description -> AAV serotype 9 delivering gRNA 2 + CB SaCas9 targeting the Ai9 locus generatedDescription -> ..........AAV serotype 9 delivering gRNA 2 + CB SaCas9 targeting the Ai9 locus..AAV serotype 9 delivering ; sgRNA 1 + CB SaCas9 targeting the Ai9 locus....CAG-loxP-stop-loxP-tdtomato reporter cassette which is ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to AAV serotype 9 delivering gRNA 2 + CB SaCas9 targeting the Ai9 locus View Associated:
AAVcc47-Ai9-sgRNA1-CB-SaCas9 - Vector Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) description -> AAVcc47 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus generatedDescription -> ..........AAVcc47 delivering sgRNA 2 + CB SaCas9 targeting the Ai9 locus..AAVcc47 delivering sgRNA 1 ; + CB SaCas9 targeting the Ai9 locus....CAG-loxP-stop-loxP-tdtomato reporter cassette which is activated ; by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to AAVcc47 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus View Associated:
AAVcc47-Ai9-sgRNA1 + sgRNA2 - Vector Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) description -> AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus generatedDescription -> ..............AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus. ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus View Associated: Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter)
g-loxP2_C9 - Guide - [In Vivo] Matched on: description -> This sgRNA targets the Ai9 and related transgenes generatedDescription -> This sgRNA targets the Ai9 and related transgenes ....Shuttle peptide used to deliver reagents to airway Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene This sgRNA targets the Ai9 and related transgenes View Associated:
Cre recombinase - Genome Editor Matched on: Name -> Ai9 mouse (BCM) modelName -> Ai9 mouse (BCM) transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Cre recombinase delivered by AAVcc47-Cre viral vector View Associated:
AAVcc47_pTR_self comp 2xU6-Ai9 guides - Vector Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) description -> AAVcc47 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 transgene generatedDescription -> SaCas9..AAVcc47 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to AAVcc47 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 transgene View Associated:
AB_1196615 - Antibody - [In Vivo] Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 and related transgenes ..AAV2/5 expressing SpyCas9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene GFP (D5.1) XP Rabbit mAb antibody View Associated:
AAV-CMV-SaCas9-U6-modified scaffold-Sa-R2 - Vector Matched on: Name -> Ai9 mouse immortalized fibroblasts modelName -> Ai9 mouse immortalized fibroblasts transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> ........This gRNA targets the Ai9 and related transgenes ..AAV shuttle vector with CMV promoter driven ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene experimentName -> Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA with modified scaffold sequence View Associated:
L2-modified - Guide Matched on: description -> This gRNA targets the Ai9 and related transgenes This gRNA targets the Ai9 and related transgenes
L3-unmodified - Guide Matched on: description -> This gRNA targets the Ai9 and related transgenes This gRNA targets the Ai9 and related transgenes
R1-modified - Guide Matched on: description -> This gRNA targets the Ai9 and related transgenes This gRNA targets the Ai9 and related transgenes
sgAi9L - Guide - [In Vitro] Matched on: Name -> HEK-293T with Ai9 transient reporter assay modelName -> HEK-293T with Ai9 transient reporter assay transgene -> Ai9 plasmid description -> This sgRNA targets the Ai9 and related transgenes generatedDescription -> This sgRNA targets the Ai9 and related transgenes ..AAV2/5 expressing SpyCas9. ; HA-SV40NLS-SpCas9-SV40NLS..Lipid nanoparticle..HEK-293T cells transfected with an Ai9 inducible transgene Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene This sgRNA targets the Ai9 and related transgenes View Associated:
sgAi9L - Guide Matched on: description -> This sgRNA targets the Ai9 and related transgenes This sgRNA targets the Ai9 and related transgenes
sgAi9R - Guide - [In Vitro] Matched on: Name -> HEK-293T with Ai9 transient reporter assay modelName -> HEK-293T with Ai9 transient reporter assay transgene -> Ai9 plasmid description -> This sgRNA targets the Ai9 and related transgenes generatedDescription -> This sgRNA targets the Ai9 and related transgenes ..AAV2/5 expressing SpyCas9. ; HA-SV40NLS-SpCas9-SV40NLS..Lipid nanoparticle..HEK-293T cells transfected with an Ai9 inducible transgene Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene This sgRNA targets the Ai9 and related transgenes View Associated:
SaCas9-Lagor - Genome Editor Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> .......................AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 ; locus..AAVcc47 delivering sgRNA 2 + CB SaCas9 targeting the Ai9 locus..AAV serotype 9 delivering gRNA ; 2 + CB SaCas9 targeting the Ai9 locus..AAVcc47 delivering CMV driven SaCas9..AAV serotype 9 delivering ; u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 transgene..AAV serotype ; 9 delivering CMV driven SaCas9..AAVcc47 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus..AAV experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 View Associated: Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter) Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.
AAV9-CMV-Cre - Vector Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName -> Cre Recombinase dose escalation study in Ai9 mice AAV serotype 9 delivering CMV Cre Recombinase View Associated:
AAV9_pTR_self comp 2xU6-Ai9 guides - Vector Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) description -> AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 generatedDescription -> AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 transgene View Associated:
AAV.pU1a-SpCas9 - Vector Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 and related transgenes ..AAV2/5 expressing SpyCas9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Expresses codon-optimized SpCas9 in mammalian cells. HA-SV40NLS-SpCas9-SV40NLS View Associated:
H509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1) - Vector Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 and related transgenes ..AAV2/5 expressing SpyCas9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene AAV2/5 expressing SpyCas9. AAV2/5 expressing two sgRNAs under U6 promoter and eGFP View Associated:
Testing AAV5 for activation of tdTomato in HEK293T cells - Experiment - [In Vitro] Matched on: Name -> HEK-293T with Ai9 transient reporter assay modelName -> HEK-293T with Ai9 transient reporter assay transgene -> Ai9 plasmid description -> AAV shuttle plasmids expressing SpCas9 and guide RNAs targeting the Ai9 transgene were tested in HEK293T generatedDescription -> AAV shuttle plasmids expressing SpCas9 and guide RNAs targeting the Ai9 transgene were tested in HEK293T ; recombinase delivered by scAAV5-Cre-GFP plasmid..HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 ; HA-SV40NLS-SpCas9-SV40NLS..Lipid nanoparticle..HEK-293T cells transfected with an Ai9 inducible transgene ; recombinase delivered by scAAV5-Cre-GFP plasmid..HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 ; HA-SV40NLS-SpCas9-SV40NLS..Lipid nanoparticle..HEK-293T cells transfected with an Ai9 inducible transgene Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Gao Guang-Ping AAV shuttle plasmids expressing SpCas9 and guide RNAs targeting the Ai9 transgene were tested in HEK293T cells by transient transfection. Both delivery and gene editing were detected by fluorescence. View Associated:
L1-modified - Guide Matched on: description -> This gRNA targets the Ai9 and related transgenes This gRNA targets the Ai9 and related transgenes
R1-unmodified - Guide Matched on: description -> This gRNA targets the Ai9 and related transgenes This gRNA targets the Ai9 and related transgenes
R2-unmodified - Guide Matched on: description -> This gRNA targets the Ai9 and related transgenes This gRNA targets the Ai9 and related transgenes
AAV-CMV-SaCas9-U6-modified scaffold-Sa-L2 - Vector Matched on: Name -> Ai9 mouse immortalized fibroblasts modelName -> Ai9 mouse immortalized fibroblasts transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> ......This gRNA targets the Ai9 and related transgenes ..AAV shuttle vector with CMV promoter driven ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene experimentName -> Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA with modified scaffold sequence View Associated:
AAVcc47-Cre - Vector Matched on: Name -> Ai9 mouse (BCM) modelName -> Ai9 mouse (BCM) transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 AAV2/5 expressing Cre recombinase View Associated:
AAV9-Ai9-sgRNA1 + sgRNA2 - Vector Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) description -> AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus generatedDescription -> ..................AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus View Associated: Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter)
AAVcc47-Ai9-sgRNA2-CB-SaCas9 - Vector Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) description -> AAVcc47 delivering sgRNA 2 + CB SaCas9 targeting the Ai9 locus generatedDescription -> ..........AAVcc47 delivering sgRNA 2 + CB SaCas9 targeting the Ai9 locus..AAVcc47 delivering sgRNA 1 ; + CB SaCas9 targeting the Ai9 locus....CAG-loxP-stop-loxP-tdtomato reporter cassette which is activated ; by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to AAVcc47 delivering sgRNA 2 + CB SaCas9 targeting the Ai9 locus View Associated:
AAVcc47-CMV-SaCas9 - Vector Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> ..............AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus. ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 AAVcc47 delivering CMV driven SaCas9 View Associated: Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter)
BI28:AAV-GFAP-NLS-GFP-WPRE-synpA-L1-R2 - Vector Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> ........................................This gRNA targets the Ai9 and related transgenes ..Novel engineered ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene experimentName -> Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. Novel engineered AAV BI28 variant expressing NLS-GFP driven by the glial fibrillary acidic protein (GFAP) promoter and dual sgRNAs with modified scaffolds View Associated: Novel AAVs Engineered for Efficient and Noninvasive Cross-Species Gene Editing Throughout the Central Nervous System BCM-Rice Resource for the Analysis of Somatic Gene Editing in Mice - Deverman Validation (Deverman, Benjamin) Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. Second site validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain
SpCas9 - Genome Editor Matched on: Name -> Ai9 mouse ; HEK-293T with Ai9 transient reporter assay modelName -> Ai9 mouse ; HEK-293T with Ai9 transient reporter assay transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) ; Ai9 plasmid generatedDescription -> HA-SV40NLS-SpCas9-SV40NLS..HA-SV40NLS-SpCas9-SV40NLS..HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 ; strand breaks on both sides of the stop cassette and NHEJ repair ..HEK-293T cells transfected with an Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene HA-SV40NLS-SpCas9-SV40NLS View Associated: Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases A Novel Human T-Cell Platform to Define Biological Effects of Genome Editing Testing AAV5 for activation of tdTomato in HEK293T cells Testing AAV5 for activation of tdTomato in mouse airway A novel human T cell platform to define biological adverse effects of genome editing
AB_2209751 - Antibody - [In Vivo] Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 ; and related transgenes ..This sgRNA targets the Ai9 and related transgenes at multiple sites..AAV2/5 ; The att site flanked neo selection cassette has been removed in this strain...Ai9 mouse has a loxP-flanked Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Anti-RFP (RABBIT) Antibody View Associated: Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases The Jackson Laboratory Gene Editing Testing Center - Gong Validation (Gong, Shaoqin) Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Testing AAV5 for activation of tdTomato in mouse airway
L1-unmodified - Guide Matched on: description -> This gRNA targets the Ai9 and related transgenes This gRNA targets the Ai9 and related transgenes
L2-unmodified - Guide Matched on: description -> This gRNA targets the Ai9 and related transgenes This gRNA targets the Ai9 and related transgenes
L3-modifed - Guide Matched on: description -> This gRNA targets the Ai9 and related transgenes This gRNA targets the Ai9 and related transgenes
R2-modified - Guide Matched on: description -> This gRNA targets the Ai9 and related transgenes This gRNA targets the Ai9 and related transgenes
AAV-CMV-SaCas9-U6-gRNA-LoxP-Sa-L3 - Vector Matched on: Name -> Ai9 mouse immortalized fibroblasts modelName -> Ai9 mouse immortalized fibroblasts transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> ........This gRNA targets the Ai9 and related transgenes ..AAV shuttle vector with CMV promoter driven ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene experimentName -> Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA View Associated:
AAV-CMV-SaCas9-U6-modified scaffold-Sa-L3 - Vector Matched on: Name -> Ai9 mouse immortalized fibroblasts modelName -> Ai9 mouse immortalized fibroblasts transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> ....This gRNA targets the Ai9 and related transgenes ..AAV shuttle vector with CMV promoter driven SaCas9 ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene experimentName -> Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA with modified scaffold sequence View Associated:
AAV-CMV-SaCas9-U6-modified scaffold-Sa-LoxP2 - Vector Matched on: Name -> Ai9 mouse immortalized fibroblasts modelName -> Ai9 mouse immortalized fibroblasts transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> ..This gRNA targets the mTmG, Ai9 and related transgenes at two sites..AAV shuttle vector with CMV promoter ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus -> Ai9/Ai14/mTmG and related reporter transgene (within loxP) guideTargetLocus -> Ai9/Ai14/mTmG and related reporter transgene (within loxP) experimentName -> Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA with modified scaffold sequence View Associated:
AAV9-CMV-SaCas9 - Vector Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> .......................AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 ; AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 ; SaCas9..AAVcc47 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 AAV serotype 9 delivering CMV driven SaCas9 View Associated: Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.
BI28:AAV-GFAP-SaCas9-WPRE3-pA - Vector Matched on: Name -> Ai9 mouse modelName -> Ai9 mouse transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> ................................This gRNA targets the Ai9 and related transgenes ..Novel engineered AAV ; activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair ..Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene experimentName -> Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. Novel engineered AAV BI28 variant expressing S. aureus Cas9 driven by the glial fibrillary acidic protein (GFAP) promoter View Associated: Novel AAVs Engineered for Efficient and Noninvasive Cross-Species Gene Editing Throughout the Central Nervous System BCM-Rice Resource for the Analysis of Somatic Gene Editing in Mice - Deverman Validation (Deverman, Benjamin) Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. Second site validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain
pAAV.pU1a-SpCas9 - Vector Matched on: Name -> HEK-293T with Ai9 transient reporter assay modelName -> HEK-293T with Ai9 transient reporter assay transgene -> Ai9 plasmid generatedDescription -> HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 and related transgenes ..AAV2/5 expressing SpyCas9 ; HA-SV40NLS-SpCas9-SV40NLS..Lipid nanoparticle..HEK-293T cells transfected with an Ai9 inducible transgene Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Expresses codon-optimized SpCas9 in mammalian cells. HA-SV40NLS-SpCas9-SV40NLS View Associated:
Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea - Experiment - [In Vitro] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple ; It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Chen Zheng-Yi LNP delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea (inner ear) View Associated:
Alt-R® S.p. Cas9 Nuclease V3 - Genome Editor Matched on: generatedDescription -> ....This sgRNA targets the Ai9 and related transgenes ....Shuttle peptide used to deliver reagents to Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene View Associated:
Ai14 gRNA - Guide - [In Vivo] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) description -> This sgRNA targets the Ai9 and related transgenes at multiple sites generatedDescription -> This sgRNA targets the Ai9 and related transgenes at multiple sites....The nanocapsule is a thin glutathione Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene This sgRNA targets the Ai9 and related transgenes at multiple sites View Associated: The Jackson Laboratory Gene Editing Testing Center - Gong Validation (Gong, Shaoqin) Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
Ai14 mouse primary fibroblasts - Model System - [In Vitro] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Primary fibroblasts from Ai14 mice View Associated:
AB_2286684 - Antibody - [In Vivo] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Sox-2 (Y-17) Antibody, Santa Cruz Biotechnology View Associated:
SaLoxP1-modified - Guide Matched on: description -> This gRNA targets the mTmG, Ai9 and related transgenes at two sites This gRNA targets the mTmG, Ai9 and related transgenes at two sites
SaLoxP2-unmodified - Guide Matched on: description -> This gRNA targets the mTmG, Ai9 and related transgenes at two sites This gRNA targets the mTmG, Ai9 and related transgenes at two sites
AAV-CMV-SaCas9-U6-gRNA-LoxP-Sa-L1 - Vector Matched on: Name -> Ai9 mouse immortalized fibroblasts modelName -> Ai9 mouse immortalized fibroblasts transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> ......This gRNA targets the Ai9 and related transgenes ..AAV shuttle vector with CMV promoter driven ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene experimentName -> Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA View Associated:
AAV-CMV-SaCas9-U6-gRNA-LoxP-Sa-LoxP1 - Vector Matched on: Name -> Ai9 mouse immortalized fibroblasts modelName -> Ai9 mouse immortalized fibroblasts transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> ..This gRNA targets the mTmG, Ai9 and related transgenes at two sites..AAV shuttle vector with CMV promoter ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus -> Ai9/Ai14/mTmG and related reporter transgene (within loxP) guideTargetLocus -> Ai9/Ai14/mTmG and related reporter transgene (within loxP) experimentName -> Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA View Associated:
AAV-CMV-SaCas9-U6-gRNA-LoxP-Sa-R1 - Vector Matched on: Name -> Ai9 mouse immortalized fibroblasts modelName -> Ai9 mouse immortalized fibroblasts transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> ........This gRNA targets the Ai9 and related transgenes ..AAV shuttle vector with CMV promoter driven ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene experimentName -> Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA View Associated:
AAV-CMV-SaCas9-U6-modified scaffold-Sa-LoxP1 - Vector Matched on: Name -> Ai9 mouse immortalized fibroblasts modelName -> Ai9 mouse immortalized fibroblasts transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> ..This gRNA targets the mTmG, Ai9 and related transgenes at two sites..AAV shuttle vector with CMV promoter ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus -> Ai9/Ai14/mTmG and related reporter transgene (within loxP) guideTargetLocus -> Ai9/Ai14/mTmG and related reporter transgene (within loxP) experimentName -> Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA with modified scaffold sequence View Associated:
AAV-CMV-SaCas9-U6-modified scaffold-Sa-R1 - Vector Matched on: Name -> Ai9 mouse immortalized fibroblasts modelName -> Ai9 mouse immortalized fibroblasts transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> ........This gRNA targets the Ai9 and related transgenes ..AAV shuttle vector with CMV promoter driven ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene experimentName -> Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA with modified scaffold sequence View Associated:
Lipofectamine 3000 - Delivery System - [In Vitro] Matched on: Name -> HEK-293T with Ai9 transient reporter assay modelName -> HEK-293T with Ai9 transient reporter assay transgene -> Ai9 plasmid generatedDescription -> targeting eGFP compatible with CasΦ-2..Guide targeting eGFP compatible with CasΦ-1..This sgRNA targets the Ai9 ; -1 and spacer for editing in human cells. ..Lipid nanoparticle..HEK-293T cells transfected with an Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Lipid nanoparticle View Associated: Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems Testing AAV5 for activation of tdTomato in HEK293T cells Testing newly discovered Biggie Phage editors in human cells
Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia - Experiment - [In Vivo] Matched on: generatedDescription -> of GFP protein by GFP positive cells compared to DAPI stained cells...........This sgRNA targets the Ai9 ; and related transgenes ..This sgRNA targets the Ai9 and related transgenes at two sites....Shuttle peptide ; EGFP (mG) fluorescence expression replacing the red fluorescence............This sgRNA targets the Ai9 ; and related transgenes ..This sgRNA targets the Ai9 and related transgenes at two sites....Shuttle peptide Guide Target Locus -> Ai9/Ai14 reporter transgene (within loxP) ; Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene (within loxP) ; Ai9/Ai14 reporter transgene McCray Paul B In vivo shuttle peptide delivery of Cas9 and Cas12a RNPs in mouse airway epithelia. Gene editing was quantified by the GFP+ cells in large and small airways following 1 delivery of GFP protein by GFP positive cells compared to DAPI stained cells. View Associated:
Cre recombinase - Genome Editor Matched on: Name -> HEK-293T with Ai9 transient reporter assay modelName -> HEK-293T with Ai9 transient reporter assay transgene -> Ai9 plasmid generatedDescription -> recombinase delivered by scAAV5-Cre-GFP plasmid......Lipid nanoparticle..HEK-293T cells transfected with an Ai9 Cre recombinase delivered by scAAV5-Cre-GFP plasmid View Associated:
Cre recombinase - Genome Editor Matched on: Name -> Ai9 mouse immortalized fibroblasts modelName -> Ai9 mouse immortalized fibroblasts transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 experimentName -> Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus Cre recombinase delivered by pAAV-EF1a-Cre plasmid View Associated:
AAV-CMV-SaCas9-U6-gRNA-LoxP-Sa-L2 - Vector Matched on: Name -> Ai9 mouse immortalized fibroblasts modelName -> Ai9 mouse immortalized fibroblasts transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> ......This gRNA targets the Ai9 and related transgenes ..AAV shuttle vector with CMV promoter driven ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene experimentName -> Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA View Associated:
AAV-CMV-SaCas9-U6-gRNA-LoxP-Sa-LoxP2 - Vector Matched on: Name -> Ai9 mouse immortalized fibroblasts modelName -> Ai9 mouse immortalized fibroblasts transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> ..This gRNA targets the mTmG, Ai9 and related transgenes at two sites..AAV shuttle vector with CMV promoter ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus -> Ai9/Ai14/mTmG and related reporter transgene (within loxP) guideTargetLocus -> Ai9/Ai14/mTmG and related reporter transgene (within loxP) experimentName -> Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA View Associated:
AAV-CMV-SaCas9-U6-gRNA-LoxP-Sa-R2 - Vector Matched on: Name -> Ai9 mouse immortalized fibroblasts modelName -> Ai9 mouse immortalized fibroblasts transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> ........This gRNA targets the Ai9 and related transgenes ..AAV shuttle vector with CMV promoter driven ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene experimentName -> Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA View Associated:
AAV-CMV-SaCas9-U6-modified scaffold-Sa-L1 - Vector Matched on: Name -> Ai9 mouse immortalized fibroblasts modelName -> Ai9 mouse immortalized fibroblasts transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> ......This gRNA targets the Ai9 and related transgenes ..AAV shuttle vector with CMV promoter driven ; strand breaks on both sides of the stop cassette and NHEJ repair ..Immortalized fibroblasts made from Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene experimentName -> Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus AAV shuttle vector with CMV promoter driven SaCas9 and U6 promoter driven gRNA with modified scaffold sequence View Associated:
SaLoxP1-unmodified - Guide Matched on: description -> This gRNA targets the mTmG, Ai9 and related transgenes at two sites This gRNA targets the mTmG, Ai9 and related transgenes at two sites
CleanCap® Cas9 mRNA (5moU) - Genome Editor Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene SpCas9 mRNA with 2 NLS signals, HA tag and capped using CleanCap. It is polyadenylated, substituted with a modified uridine and optimized for mammalian systems. It mimics a fully processed mature mRNA. View Associated: Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
sNLS-SpCas9-sNLS - Genome Editor Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene SpCas9 with N- and C-terminal SV40 NLS View Associated: The Jackson Laboratory Gene Editing Testing Center - Gong Validation (Gong, Shaoqin) Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
Ai14 mouse - Model System - [In Vivo] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain. View Associated:
g-loxPbot_C12a - Guide - [In Vivo] Matched on: description -> This sgRNA targets the Ai9 and related transgenes at two sites generatedDescription -> This sgRNA targets the Ai9 and related transgenes at two sites....Shuttle peptide used to deliver reagents Guide Target Locus -> Ai9/Ai14 reporter transgene (within loxP) guideTargetLocus -> Ai9/Ai14 reporter transgene (within loxP) This sgRNA targets the Ai9 and related transgenes at two sites View Associated:
sg298 - Guide - [In Vivo, In Vitro] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) description -> This sgRNA targets the Ai9 and related transgenes at multiple sites generatedDescription -> This sgRNA targets the Ai9 and related transgenes at multiple sites....combinatorial library cationic Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene This sgRNA targets the Ai9 and related transgenes at multiple sites View Associated: Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea
SaLoxP2-modified - Guide Matched on: description -> This gRNA targets the mTmG, Ai9 and related transgenes at two sites This gRNA targets the mTmG, Ai9 and related transgenes at two sites
SpCas9 - Genome Editor Matched on: Name -> Ai9 mouse (BCM) modelName -> Ai9 mouse (BCM) transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> two sites, or Cre recombinase between loxP sites, tdTomato expression is lost and GFP is expressed...Ai9 View Associated:
pH509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1) - Vector Matched on: Name -> HEK-293T with Ai9 transient reporter assay modelName -> HEK-293T with Ai9 transient reporter assay transgene -> Ai9 plasmid generatedDescription -> ..HA-SV40NLS-SpCas9-SV40NLS..This sgRNA targets the Ai9 and related transgenes ..AAV2/5 expressing SpyCas9 ; HA-SV40NLS-SpCas9-SV40NLS..Lipid nanoparticle..HEK-293T cells transfected with an Ai9 inducible transgene Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene AAV2/5 expressing SpyCas9. AAV2/5 expressing two sgRNAs under U6 promoter and eGFP View Associated:
mTmG mouse - Model System - [In Vivo] Matched on: generatedDescription -> ........This sgRNA targets the Ai9 and related transgenes ..This sgRNA targets the Ai9 and related transgenes Guide Target Locus -> Ai9/Ai14 reporter transgene (within loxP) ; Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene (within loxP) ; Ai9/Ai14 reporter transgene ROSAmT/mG is a cell membrane-targeted, two-color fluorescent Cre-reporter allele. Prior to Cre recombination, cell membrane-localized tdTomato (mT) fluorescence expression is widespread in cells/tissues. Cre recombinase expressing cells (and future cell lineages derived from these cells) have cell membrane-localized EGFP (mG) fluorescence expression replacing the red fluorescence View Associated:
AsCas12a (IDT and Feldan Therapeutics) - Genome Editor Matched on: generatedDescription -> ....This sgRNA targets the Ai9 and related transgenes at two sites....Shuttle peptide used to deliver Guide Target Locus -> Ai9/Ai14 reporter transgene (within loxP) guideTargetLocus -> Ai9/Ai14 reporter transgene (within loxP) View Associated:
Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea - Experiment - [In Vivo] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple ; It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Chen Zheng-Yi Delivery of Cas9/sgRNA mRNA via new LNPs to the cochlea by cochleostomy and gene editing is measured by percentage of tdTomato positive cells. View Associated:
CleanCap® Cas9 - Genome Editor Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene SpCas9 mRNA with 2 NLS signals, HA tag and capped using CleanCap. It is polyadenylated, substituted with a modified uridine and optimized for mammalian systems. It mimics a fully processed mature mRNA. View Associated: Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea
306-O12B - Delivery System - [In Vivo, In Vitro] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple ; sites..This sgRNA targets the Ai9 and related transgenes at multiple sites. 2'-O-Methyl at 3 first Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Combinatorial library cationic lipid nanoparticles View Associated: Efficient In Vivo RNP-Based Gene Editing in the Sensory Organ Inner Ear Using Bioreducible Lipid Nanoparticles The Jackson Laboratory Gene Editing Testing Center - Chen Validation (Chen, Zheng-Yi) Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea Testing new LNPs (lipid nanoparticles) for delivery of Fluc mRNA in adult mice Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea
RNP-NC-CPP - Delivery System - [In Vivo] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a cell penetrating peptide (CPP) from the TAT peptide (GRKKRRQRRRPQ) which lacks cell-type specficity View Associated: The Jackson Laboratory Gene Editing Testing Center - Gong Validation (Gong, Shaoqin) Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
AB_10015251 - Antibody - [In Vivo] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Anti-Myosin VIIa antibody, Proteus Biosciences View Associated:
[] Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain - Experiment - [In Vivo] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 ; SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Murray Stephen A Delivery of CRISPR/Cas9 via RNP-loaded nanocages to the brain in Ai14 mice View Associated:
113-O12B - Delivery System - [In Vivo] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene combinatorial library cationic lipid nanoparticles View Associated: Testing new LNPs (lipid nanoparticles) for delivery of Fluc mRNA in adult mice Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea
Ai14 mouse (congenic) - Model System - [In Vivo] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> mimics a fully processed mature mRNA...SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 ; and related transgenes at multiple sites..This sgRNA targets the Ai9 and related transgenes at multiple Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain. View Associated: Efficient In Vivo RNP-Based Gene Editing in the Sensory Organ Inner Ear Using Bioreducible Lipid Nanoparticles The Jackson Laboratory Gene Editing Testing Center - Gong Validation (Gong, Shaoqin) The Jackson Laboratory Gene Editing Testing Center - Chen Validation (Chen, Zheng-Yi) Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea
AB_2532994 - Antibody - [In Vivo] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Thermo-Fisher, Rat anti-GFAP View Associated:
AB_141637 - Antibody - [In Vivo] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Invitrogen, Donkey anti-rabbit alexa fluor 594 View Associated:
AB_141607 - Antibody - [In Vivo] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Invitrogen, Donkey anti-mouse alexafluor 488 View Associated:
Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. - Experiment - [In Vivo] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 ; SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Gong Shaoqin (Sarah) Nanocapusules carrying CRISPR Cas9 RNP with guide RNA targeting the stop sequence in the Ai14 transgene are intracerebrally delivered to Ai14 mice and gene editing is measured by gain of tdTomato protein expression. View Associated:
[] Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear - Experiment - [In Vivo] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple ; It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Murray Stephen A Delivery of CRISPR/Cas9 via bioreducible lipid nanoparticles (LNPs) to the inner ear in Ai14 mice View Associated:
[] Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear - Experiment - [In Vivo] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple ; It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Murray Stephen A Delivery of CRISPR/Cas9 editor via bioreducible lipid nanoparticle to the inner ear in Ai14 mice View Associated:
306-S10 - Delivery System - [In Vivo, In Vitro] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> It mimics a fully processed mature mRNA...This sgRNA targets the Ai9 and related transgenes at multiple ; sites..This sgRNA targets the Ai9 and related transgenes at multiple sites. 2'-O-Methyl at 3 first Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene combinatorial library cationic lipid nanoparticles View Associated: Efficient In Vivo RNP-Based Gene Editing in the Sensory Organ Inner Ear Using Bioreducible Lipid Nanoparticles The Jackson Laboratory Gene Editing Testing Center - Chen Validation (Chen, Zheng-Yi) Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea Testing new LNPs (lipid nanoparticles) for delivery of Fluc mRNA in adult mice Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea
RNP-NC-no ligand - Delivery System - [In Vivo] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. View Associated: The Jackson Laboratory Gene Editing Testing Center - Gong Validation (Gong, Shaoqin) Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
RNP-NC-RVG - Delivery System - [In Vivo] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a RVG peptide YTIWMPENPRPGTPCDIFTNSRGKRASNG which specifically interacts withthe N-acetylecholine receptor (AchR) on neuronal cells, which mediates NP entry View Associated:
sg298 - Guide - [In Vivo] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) description -> This sgRNA targets the Ai9 and related transgenes at multiple sites. 2'-O-Methyl at 3 first and last generatedDescription -> This sgRNA targets the Ai9 and related transgenes at multiple sites. 2'-O-Methyl at 3 first and last Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene This sgRNA targets the Ai9 and related transgenes at multiple sites. 2'-O-Methyl at 3 first and last bases, 3' phosphorothioate bonds between first 3 and last 2 bases View Associated: Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
AB_10711040 - Antibody - [In Vivo] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Abcam, mouse anti-NeuN View Associated:
AB_2813835 - Antibody - [In Vivo] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) generatedDescription -> SpCas9 with N- and C-terminal SV40 NLS..SpCas9 with N- and C-terminal SV40 NLS..This sgRNA targets the Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene Abcam, Donkey anti-rat alexa fluour 647 View Associated:
S10 - Delivery System - [In Vivo, In Vitro] Matched on: generatedDescription -> C-Myc-like NLS at the N-terminal SV40 NLS and Nucleoplasmin NLS at the C-terminal..This sgRNA targets the Ai9 ; transgenes..gRNA targeting human NKG2A exon 3..gRNA targeting CFTR inton-22-23..This sgRNA targets the Ai9 Guide Target Locus -> Ai9/Ai14 reporter transgene (within loxP) ; Ai9/Ai14 reporter transgene guideTargetLocus -> Ai9/Ai14 reporter transgene (within loxP) ; Ai9/Ai14 reporter transgene Shuttle peptide used to deliver reagents to airway epithelia View Associated: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Testing Shuttle Peptides ability to deliver GFP-NLS to airway epithelia. Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells. Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells Gene editing in vitro by various peptide variants delivering Cas12a in NK cells.
306-O12B blank - Delivery System - [In Vivo] Matched on: transgene -> Ai9 (CAG-lox-stop-lox-tdTomato) Combinatorial library cationic lipid nanoparticles View Associated:

115 results for ai9

Category	Type	Name	Description	View Associated..
Model System	Animal	Ai9 mouse	Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus.	Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases Novel AAVs Engineered for Efficient and Noninvasive Cross-Species Gene Editing Throughout the Central Nervous System Evolving High Potency AAV Vectors for Neuromuscular Genome Editing BCM-Rice Resource for the Analysis of Somatic Gene Editing in Mice - Deverman Validation (Deverman, Benjamin) Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter) Testing AAV5 for activation of tdTomato in mouse airway Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) Cre Recombinase dose escalation study in Ai9 mice Second site validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.
Model System	Animal	Ai9 mouse (BCM)	Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus.
Model System	Cell	Ai9 mouse immortalized fibroblasts	Immortalized fibroblasts made from Ai9 (B6.Cg-Gt(ROSA)26Sor^tm9(CAG-tdTomato)Hze/J) mice
Guide		Ai9 SaCas9 Guide B	This gRNA targets the Ai9 and related transgenes
Guide		Ai9 SaCas9 Guide A	This gRNA targets the Ai9 and related transgenes
Model System	Cell	HEK-293T with Ai9 transient reporter assay	HEK-293T cells transfected with an Ai9 inducible transgene reporter plasmid used to test gene editing activity by fluorescence
Experiment	In Vivo	Cre Recombinase dose escalation study in Ai9 mice	A single stranded cmv cre cassette was packaged into AAV9 or AAVcc47 and injected intravenously in Ai9 mice. We injected n=3 at three different doses (1e10, 1e11, 1e12 vg) and harvested organs 4 weeks post injection. Fluorescence intensity in liver, heart, and skeletal muscle was quantified with tiff based images in Image J and neuronal transduction from each vector was quantified at the 1e12vg dose by counting the number of tdTomato+ neurons and number of NeuN+ cells from multiple sections and images.
Experiment	In Vivo	Testing gRNA sequence and gRNA scaffold modified in Ai9 mice.	3e11 vg/mouse of AAV-BI28:GFAP-SaCas9-WPRE-pA and 3e11 vg/mouse of AAV-BI28:GFAP-NLS-GFP-U6-L1-U6-R2 were codelivered intravenously to adult male and female Ai9 mice. Editing was assessed in brain sections 4 weeks later.
Experiment	In Vitro	Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus in vitro	Reporter transgene activation by SaCas9 gRNA target and modified scaffold sequences by transient transfection in immortalized Ai9 mouse fibroblasts
Experiment	In Vivo	Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter)	A dual vector strategy was employed: one delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (both single stranded AAV cassettes). This strategy was evaluted with both AAV9 (n=3) and AAVcc47 (n=3) by intravenous injection in Ai9 mice. A total dose of 3e12vg was injected into each mouse (1.5e12vg each vector mixed 1:1) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
Experiment	In Vivo	Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter)	A dual vector strategy was employed: one delivering a single guide RNA and CB driven SaCas9, and another delivering the second guide RNA and CB driven SaCas9. This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice. A total dose of 2e12vg was injected into each mouse (1e12vg each vector mixed 1:1) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
Experiment	In Vivo	Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter)	A dual vector strategy was employed: one delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (both single stranded AAV cassettes). This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice. A total dose of 4e12vg was injected into each mouse and vectors mixed in a 1:4 ratio of cas9 to guide RNA (1e12vg of CMV Sacas9 vector and 3e12vg of the sgRNA vector) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
Experiment	In Vivo	Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.	A dual vector strategy was employed: one self complementary vector delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (single stranded vector). This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=4) by intravenous injection in Ai9 mice. A total dose of 4e12vg was injected into each mouse and vectors mixed in a 1:1 ratio of cas9 to guide RNA (2e12vg of CMV Sacas9 vector and 2e12vg of the self complementary sgRNA vector) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
Experiment	In Vivo	Validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing.	Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery. Detection of editing in non-target tissues.
Genome Editor	Cas Nuclease	SauCas9
Vector	Viral Vector	AAVcc47-SaCas9-Ai9	AAV2/9 expressing SaCas9 and single sgRNA under U6 promoter
Genome Editor	Cas Nuclease	SaCas9		Novel AAVs Engineered for Efficient and Noninvasive Cross-Species Gene Editing Throughout the Central Nervous System BCM-Rice Resource for the Analysis of Somatic Gene Editing in Mice - Deverman Validation (Deverman, Benjamin) Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus in vitro Second site validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain
Vector	Viral Vector	AAVcc47-CMV-Cre	AAVcc47 delivering CMV Cre Recombinase
Experiment	In Vivo	Testing AAV5 for activation of tdTomato in mouse airway	AAV2/5 mediated gene editing in the mouse airway was tested by deliverying SpCas9 and guide RNAs targeting the Ai9 transgene in Ai9 transgenic mice. Viral delivery was detected by GFP expression and gene editing quantified by tdTomato activation
Experiment	In Vivo	Second site validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain	Validation of delivery of AAV custom designed to cross the blood-brain barrier for CRISPR/Cas9 editing. Editing detected and quantified in brain by generation of tdTomato fluorescent protein signal from Ai9 reporter mice
Vector	Viral Vector	AAV9-Ai9-sgRNA1-CB-SaCas9	AAV serotype 9 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus
Vector	Viral Vector	AAV9-Ai9-sgRNA2-CB-SaCas9	AAV serotype 9 delivering gRNA 2 + CB SaCas9 targeting the Ai9 locus
Vector	Viral Vector	AAVcc47-Ai9-sgRNA1-CB-SaCas9	AAVcc47 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus

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115 results for ai9

115 results for ai9

Ai9 mouse - Model System - [In Vivo]

Ai9 mouse (BCM) - Model System - [In Vivo]

Ai9 mouse immortalized fibroblasts - Model System - [In Vitro]

Ai9 SaCas9 Guide B - Guide

Ai9 SaCas9 Guide A - Guide

HEK-293T with Ai9 transient reporter assay - Model System - [In Vitro]

Cre Recombinase dose escalation study in Ai9 mice - Experiment - [In Vivo]

Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. - Experiment - [In Vivo]

Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus in vitro - Experiment - [In Vitro]

Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) - Experiment - [In Vivo]

Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter) - Experiment - [In Vivo]

Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter) - Experiment - [In Vivo]

Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector. - Experiment - [In Vivo]

[] Validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing. - Experiment - [In Vivo]

SauCas9 - Genome Editor

AAVcc47-SaCas9-Ai9 - Vector

SaCas9 - Genome Editor

AAVcc47-CMV-Cre - Vector

Testing AAV5 for activation of tdTomato in mouse airway - Experiment - [In Vivo]

[] Second site validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain - Experiment - [In Vivo]

AAV9-Ai9-sgRNA1-CB-SaCas9 - Vector

AAV9-Ai9-sgRNA2-CB-SaCas9 - Vector

AAVcc47-Ai9-sgRNA1-CB-SaCas9 - Vector

AAVcc47-Ai9-sgRNA1 + sgRNA2 - Vector

g-loxP2_C9 - Guide - [In Vivo]

Cre recombinase - Genome Editor

AAVcc47_pTR_self comp 2xU6-Ai9 guides - Vector

AB_1196615 - Antibody - [In Vivo]

AAV-CMV-SaCas9-U6-modified scaffold-Sa-R2 - Vector

L2-modified - Guide

L3-unmodified - Guide

R1-modified - Guide

sgAi9L - Guide - [In Vitro]

sgAi9L - Guide

sgAi9R - Guide - [In Vitro]

SaCas9-Lagor - Genome Editor

AAV9-CMV-Cre - Vector

AAV9_pTR_self comp 2xU6-Ai9 guides - Vector

AAV.pU1a-SpCas9 - Vector

H509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1) - Vector

Testing AAV5 for activation of tdTomato in HEK293T cells - Experiment - [In Vitro]

L1-modified - Guide

R1-unmodified - Guide

R2-unmodified - Guide

AAV-CMV-SaCas9-U6-modified scaffold-Sa-L2 - Vector

AAVcc47-Cre - Vector

AAV9-Ai9-sgRNA1 + sgRNA2 - Vector

AAVcc47-Ai9-sgRNA2-CB-SaCas9 - Vector

AAVcc47-CMV-SaCas9 - Vector

BI28:AAV-GFAP-NLS-GFP-WPRE-synpA-L1-R2 - Vector

SpCas9 - Genome Editor

AB_2209751 - Antibody - [In Vivo]

L1-unmodified - Guide

L2-unmodified - Guide

L3-modifed - Guide

R2-modified - Guide

AAV-CMV-SaCas9-U6-gRNA-LoxP-Sa-L3 - Vector

AAV-CMV-SaCas9-U6-modified scaffold-Sa-L3 - Vector

AAV-CMV-SaCas9-U6-modified scaffold-Sa-LoxP2 - Vector

AAV9-CMV-SaCas9 - Vector

BI28:AAV-GFAP-SaCas9-WPRE3-pA - Vector

pAAV.pU1a-SpCas9 - Vector

Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea - Experiment - [In Vitro]

Alt-R® S.p. Cas9 Nuclease V3 - Genome Editor

Ai14 gRNA - Guide - [In Vivo]

Ai14 mouse primary fibroblasts - Model System - [In Vitro]

AB_2286684 - Antibody - [In Vivo]

SaLoxP1-modified - Guide

SaLoxP2-unmodified - Guide

AAV-CMV-SaCas9-U6-gRNA-LoxP-Sa-L1 - Vector

AAV-CMV-SaCas9-U6-gRNA-LoxP-Sa-LoxP1 - Vector

AAV-CMV-SaCas9-U6-gRNA-LoxP-Sa-R1 - Vector

AAV-CMV-SaCas9-U6-modified scaffold-Sa-LoxP1 - Vector

AAV-CMV-SaCas9-U6-modified scaffold-Sa-R1 - Vector

Lipofectamine 3000 - Delivery System - [In Vitro]

Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia - Experiment - [In Vivo]

Cre recombinase - Genome Editor