Genome Editors

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23 results in Genome Editors

AsCas12a (Feldan Therapeutics) - Genome Editor - [In Vitro] [Delivery Systems Initiative] [Human] Matched on: category --> Genome Editor Experiments Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells. Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells Gene editing in vitro by various peptide variants delivering Cas12a in NK cells.
CasΦ-1 - Genome Editor - [In Vitro] [Genome Editors] [Human] Matched on: category --> Genome Editor Compact editor of the Cas12j family identified in biggie phage from metagenomic assemblies Experiments Testing newly discovered Biggie Phage editors in human cells
Cre recombinase - Genome Editor - [In Vitro] [Delivery Systems Initiative] [Human] Matched on: category --> Genome Editor Cre recombinase delivered by plasmid (see vector details) Experiments Testing AAV5 for activation of tdTomato in HEK293T cells
GFP-NLS - Genome Editor - [In Vivo] [Delivery Systems Initiative] [Mouse] Matched on: category --> Genome Editor Nuclear targeted GFP Experiments Testing Shuttle Peptides ability to deliver GFP-NLS to airway epithelia.
SaCas9 - Genome Editor - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse] Matched on: category --> Genome Editor Experiments Validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice
SaCas9-Lagor - Genome Editor - [In Vivo] [Delivery Systems Initiative] [Mouse] Matched on: category --> Genome Editor Experiments Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter) Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.
SauCas9 - Genome Editor - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse] Matched on: category --> Genome Editor Experiments Validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing.
CleanCap® Cas9 mRNA (5moU) - Genome Editor - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse] Matched on: category --> Genome Editor SpCas9 mRNA with 2 NLS signals, HA tag and capped using CleanCap. It is polyadenylated, substituted with a modified uridine and optimized for mammalian systems. It mimics a fully processed mature mRNA. Experiments Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
sNLS-SpCas9-sNLS - Genome Editor - [In Vivo] [Small Animal Testing Center (SATC), Delivery Systems Initiative] [Mouse] Matched on: category --> Genome Editor SpCas9 with N- and C-terminal SV40 NLS Experiments Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
SpyCas9-3xNLS - Genome Editor - [In Vivo] [Delivery Systems Initiative] [Mouse] Matched on: category --> Genome Editor SpyCas9-3xNLS is type II-A Cas9 from Streptococcus pyogenes strain SF370. It was expressed from pMCSG7 bacterial expressing vector and purified from Escherichia coli Rosetta DE3 strain. SpyCas9 fused to 3 NLS: C-Myc-like NLS at the N-terminal SV40 NLS and Nucleoplasmin NLS at the C-terminal Experiments Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain Delivery of RNP containing chemically modified crRNA C20 with chemically modified tracrRNA T2-PS to determine the RNP distribution in TLR-MCV mouse brain Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
Alt-R® S.p. Cas9 Nuclease V3 - Genome Editor - [In Vivo] [Delivery Systems Initiative] [Mouse] Matched on: category --> Genome Editor Experiments Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia
CleanCap® Fluc - Genome Editor - [In Vivo] [Delivery Systems Initiative] [Mouse] Matched on: category --> Genome Editor Firefly luciferase mRNA capped using CleanCap. It is polyadenylated, modified with 5-methoxyuridine and optimized for mammalian systems. It mimics a fully processed mature mRNA. Experiments Testing new LNPs (lipid nanoparticles) for delivery of Fluc mRNA in adult mice
SpCas9 - Genome Editor - [In Vivo, In Vitro] [Delivery Systems Initiative, Biological Systems] [Human, Mouse] Matched on: category --> Genome Editor HA-SV40NLS-SpCas9-SV40NLS Experiments Testing AAV5 for activation of tdTomato in HEK293T cells Testing AAV5 for activation of tdTomato in mouse airway A novel human T cell platform to define biological adverse effects of genome editing Testing AAV5 for activation of tdTomato in mouse airway club and ciliated cells
SpyCas9-3xNLS - Genome Editor - [In Vivo, In Vitro] [Delivery Systems Initiative] [Human, Mouse] Matched on: category --> Genome Editor SpyCas9-3xNLS is type II-A Cas9 from Streptococcus pyogenes strain SF370. It was expressed from pMCSG7 bacterial expressing vector and purified from Escherichia coli Rosetta DE3 strain. SpyCas9 fused to 3 NLS: C-Myc-like NLS at the N-terminal SV40 NLS and Nucleoplasmin NLS at the C-terminal Experiments Testing newly chemically modified crRNA and tracrRNA in mTmG mouse embryonic fibroblasts Testing newly chemically modified crRNA and tracrRNA to activate the TLR1 reporter in human cells Testing newly chemically modified crRNA and tracrRNA in mouse Hepa 1-6 cells Testing newly chemically modified crRNA and tracrRNA in mouse Neuro 2A cells Testing preparation for validation at The Jackson Laboratory Small Animal Testing Center Testing newly chemically modified crRNA and tracrRNA to activate the TLR reporter in human cells
AsCas12a (IDT and Feldan Therapeutics) - Genome Editor - [In Vivo] [Delivery Systems Initiative] [Mouse] Matched on: category --> Genome Editor Experiments Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia
SpCas9 (Feldan Therapeutics) - Genome Editor - [In Vitro] [Delivery Systems Initiative] [Human] Matched on: category --> Genome Editor Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells.
CasΦ-2 - Genome Editor - [In Vitro] [Genome Editors] [Human] Matched on: category --> Genome Editor Compact editor of the Cas12j family identified in biggie phage from metagenomic assemblies Experiments Testing newly discovered Biggie Phage editors in human cells
CasΦ-3 - Genome Editor - [In Vitro] [Genome Editors] [Human] Matched on: category --> Genome Editor Compact editor of the Cas12j family identified in biggie phage from metagenomic assemblies Experiments Testing newly discovered Biggie Phage editors in human cells
CleanCap® Cas9 - Genome Editor - [In Vivo, In Vitro] [Delivery Systems Initiative] [Mouse] Matched on: category --> Genome Editor SpCas9 mRNA with 2 NLS signals, HA tag and capped using CleanCap. It is polyadenylated, substituted with a modified uridine and optimized for mammalian systems. It mimics a fully processed mature mRNA. Experiments Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea
Cre recombinase - Genome Editor - [In Vivo, In Vitro] [Small Animal Testing Center (SATC), Delivery Systems Initiative] [Mouse] Matched on: category --> Genome Editor Cre recombinase delivered by AAV (see vector details) Experiments Validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing. Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus in vitro Validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice
SaCas9 - Genome Editor - [In Vivo, In Vitro] [Small Animal Testing Center (SATC), Delivery Systems Initiative] [Mouse] Matched on: category --> Genome Editor Experiments Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus in vitro Second site validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain
SpCas9 - Genome Editor - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse] Matched on: category --> Genome Editor Experiments Validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
TadA-8e V106W - Genome Editor - [In Vivo] [Delivery Systems Initiative] [Mouse] Matched on: category --> Genome Editor Catalytically impaired Cas fused to evolved TadA deaminase (TadA-8e V106W) Experiments Pcsk9 adenine base editor efficiency in liver and nonliver tissue On-target editing compared to 14 circle-seq nominated off-target sites of adenine base editor delivered by BE-eVLP vs AAV in the C57BL/6 liver

23 results in Genome Editors

Category	Type	Name	Description	View Associated..
Genome Editor	Cas Nuclease	AsCas12a (Feldan Therapeutics)		Experiments Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells. Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells Gene editing in vitro by various peptide variants delivering Cas12a in NK cells.
Genome Editor	Cas Nuclease	CasΦ-1	Compact editor of the Cas12j family identified in biggie phage from metagenomic assemblies	Experiments Testing newly discovered Biggie Phage editors in human cells
Genome Editor	Recombinase	Cre recombinase	Cre recombinase delivered by plasmid (see vector details)	Experiments Testing AAV5 for activation of tdTomato in HEK293T cells
Genome Editor	Reporter	GFP-NLS	Nuclear targeted GFP	Experiments Testing Shuttle Peptides ability to deliver GFP-NLS to airway epithelia.
Genome Editor	Cas Nuclease	SaCas9		Experiments Validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice
Genome Editor	Cas Nuclease	SaCas9-Lagor		Experiments Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter) Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.
Genome Editor	Cas Nuclease	SauCas9		Experiments Validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing.
Genome Editor	Cas Nuclease	CleanCap® Cas9 mRNA (5moU)	SpCas9 mRNA with 2 NLS signals, HA tag and capped using CleanCap. It is polyadenylated, substituted with a modified uridine and optimized for mammalian systems. It mimics a fully processed mature mRNA.	Experiments Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
Genome Editor	Cas Nuclease	sNLS-SpCas9-sNLS	SpCas9 with N- and C-terminal SV40 NLS	Experiments Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
Genome Editor	Cas Nuclease	SpyCas9-3xNLS	SpyCas9-3xNLS is type II-A Cas9 from Streptococcus pyogenes strain SF370. It was expressed from pMCSG7 bacterial expressing vector and purified from Escherichia coli Rosetta DE3 strain. SpyCas9 fused to 3 NLS: C-Myc-like NLS at the N-terminal SV40 NLS and Nucleoplasmin NLS at the C-terminal	Experiments Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain Delivery of RNP containing chemically modified crRNA C20 with chemically modified tracrRNA T2-PS to determine the RNP distribution in TLR-MCV mouse brain Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
Genome Editor	Cas Nuclease	Alt-R® S.p. Cas9 Nuclease V3		Experiments Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia
Genome Editor	Reporter	CleanCap® Fluc	Firefly luciferase mRNA capped using CleanCap. It is polyadenylated, modified with 5-methoxyuridine and optimized for mammalian systems. It mimics a fully processed mature mRNA.	Experiments Testing new LNPs (lipid nanoparticles) for delivery of Fluc mRNA in adult mice
Genome Editor	Cas Nuclease	SpCas9	HA-SV40NLS-SpCas9-SV40NLS	Experiments Testing AAV5 for activation of tdTomato in HEK293T cells Testing AAV5 for activation of tdTomato in mouse airway A novel human T cell platform to define biological adverse effects of genome editing Testing AAV5 for activation of tdTomato in mouse airway club and ciliated cells
Genome Editor	Cas Nuclease	SpyCas9-3xNLS	SpyCas9-3xNLS is type II-A Cas9 from Streptococcus pyogenes strain SF370. It was expressed from pMCSG7 bacterial expressing vector and purified from Escherichia coli Rosetta DE3 strain. SpyCas9 fused to 3 NLS: C-Myc-like NLS at the N-terminal SV40 NLS and Nucleoplasmin NLS at the C-terminal	Experiments Testing newly chemically modified crRNA and tracrRNA in mTmG mouse embryonic fibroblasts Testing newly chemically modified crRNA and tracrRNA to activate the TLR1 reporter in human cells Testing newly chemically modified crRNA and tracrRNA in mouse Hepa 1-6 cells Testing newly chemically modified crRNA and tracrRNA in mouse Neuro 2A cells Testing preparation for validation at The Jackson Laboratory Small Animal Testing Center Testing newly chemically modified crRNA and tracrRNA to activate the TLR reporter in human cells
Genome Editor	Cas Nuclease	AsCas12a (IDT and Feldan Therapeutics)		Experiments Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia
Genome Editor	Cas Nuclease	SpCas9 (Feldan Therapeutics)		Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells.
Genome Editor	Cas Nuclease	CasΦ-2	Compact editor of the Cas12j family identified in biggie phage from metagenomic assemblies	Experiments Testing newly discovered Biggie Phage editors in human cells
Genome Editor	Cas Nuclease	CasΦ-3	Compact editor of the Cas12j family identified in biggie phage from metagenomic assemblies	Experiments Testing newly discovered Biggie Phage editors in human cells
Genome Editor	Cas Nuclease	CleanCap® Cas9	SpCas9 mRNA with 2 NLS signals, HA tag and capped using CleanCap. It is polyadenylated, substituted with a modified uridine and optimized for mammalian systems. It mimics a fully processed mature mRNA.	Experiments Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea
Genome Editor	Recombinase	Cre recombinase	Cre recombinase delivered by AAV (see vector details)	Experiments Validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing. Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus in vitro Validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice
Genome Editor	Cas Nuclease	SaCas9		Experiments Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. Selection of gRNA sequences and gRNA scaffold modification lead to improved editing of the Ai9 locus in vitro Second site validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain
Genome Editor	Cas Nuclease	SpCas9		Experiments Validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Genome Editor	Base Editor (SpCas9)	TadA-8e V106W	Catalytically impaired Cas fused to evolved TadA deaminase (TadA-8e V106W)	Experiments Pcsk9 adenine base editor efficiency in liver and nonliver tissue On-target editing compared to 14 circle-seq nominated off-target sites of adenine base editor delivered by BE-eVLP vs AAV in the C57BL/6 liver

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23 results in Genome Editors

AsCas12a (Feldan Therapeutics) - Genome Editor - [In Vitro] [Delivery Systems Initiative] [Human]

CasΦ-1 - Genome Editor - [In Vitro] [Genome Editors] [Human]

Cre recombinase - Genome Editor - [In Vitro] [Delivery Systems Initiative] [Human]

GFP-NLS - Genome Editor - [In Vivo] [Delivery Systems Initiative] [Mouse]

SaCas9 - Genome Editor - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]

SaCas9-Lagor - Genome Editor - [In Vivo] [Delivery Systems Initiative] [Mouse]

SauCas9 - Genome Editor - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]

CleanCap® Cas9 mRNA (5moU) - Genome Editor - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]

sNLS-SpCas9-sNLS - Genome Editor - [In Vivo] [Small Animal Testing Center (SATC), Delivery Systems Initiative] [Mouse]

SpyCas9-3xNLS - Genome Editor - [In Vivo] [Delivery Systems Initiative] [Mouse]

Alt-R® S.p. Cas9 Nuclease V3 - Genome Editor - [In Vivo] [Delivery Systems Initiative] [Mouse]

CleanCap® Fluc - Genome Editor - [In Vivo] [Delivery Systems Initiative] [Mouse]

SpCas9 - Genome Editor - [In Vivo, In Vitro] [Delivery Systems Initiative, Biological Systems] [Human, Mouse]

SpyCas9-3xNLS - Genome Editor - [In Vivo, In Vitro] [Delivery Systems Initiative] [Human, Mouse]

AsCas12a (IDT and Feldan Therapeutics) - Genome Editor - [In Vivo] [Delivery Systems Initiative] [Mouse]

SpCas9 (Feldan Therapeutics) - Genome Editor - [In Vitro] [Delivery Systems Initiative] [Human]

CasΦ-2 - Genome Editor - [In Vitro] [Genome Editors] [Human]

CasΦ-3 - Genome Editor - [In Vitro] [Genome Editors] [Human]

CleanCap® Cas9 - Genome Editor - [In Vivo, In Vitro] [Delivery Systems Initiative] [Mouse]

Cre recombinase - Genome Editor - [In Vivo, In Vitro] [Small Animal Testing Center (SATC), Delivery Systems Initiative] [Mouse]

SaCas9 - Genome Editor - [In Vivo, In Vitro] [Small Animal Testing Center (SATC), Delivery Systems Initiative] [Mouse]

SpCas9 - Genome Editor - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]

TadA-8e V106W - Genome Editor - [In Vivo] [Delivery Systems Initiative] [Mouse]

23 results in Genome Editors