Animal
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Mouse
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Ai14 mouse
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Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain. |
The Jackson Laboratory
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Animal
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Mouse
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Ai14 mouse (congenic)
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Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain. |
The Jackson Laboratory
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Cell
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Mouse
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Primary cells
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Ai14 mouse primary fibroblasts
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Primary fibroblasts from Ai14 mice |
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Animal
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Mouse
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Ai9 mouse
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Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. |
The Jackson Laboratory
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Animal
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Mouse
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Ai9 mouse (BCM)
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Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. |
Baylor College of Medicine
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Cell
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Mouse
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Immortalized Fibroblast (unspecified)
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Ai9 mouse immortalized fibroblasts
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Immortalized fibroblasts made from Ai9 (B6.Cg-Gt(ROSA)26Sor^tm9(CAG-tdTomato)Hze/J) mice |
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Animal
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Mouse
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Ai9-SauSpyCas9 mouse
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Using CRISPR/Cas9 genome editing in mouse embryos, the existing Rosa-CAG-LSL-tdTomato-WPRE conditional allele Gt(ROSA)26Sortm9(CAG-tdTomato)Hze (commonly referred to as Ai9) was modified to duplicate the guide target sequences for S. pyogenes and S. aureus Cas9 found on the 3' end of the loxP-flanked stop cassette [SpyCas9 5'GTATGCTATACGAAGTTAT (PAM AGG); SauCas9 5'ACGAAGTTATATTAAGGGTT(PAM CCGGAT)] onto the 5' end of the stop cassette. With this modification, a single guide RNA for S. pyogenes or S. aureus Cas9 can be used to mediate deletion of the stop cassette by non-homologous end joining and activation of tdTomato expression. |
Baylor College of Medicine
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Animal
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Mouse
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B6
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C57BL/6J WT mouse |
Baylor College of Medicine
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Animal
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Mouse
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BALB/c mouse
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BALB/cJ is a commonly used inbred. Key traits include a susceptibility to developing the demyelinating disease upon infection with Theiler's murine encephalomyelitis virus. The BALB/cJ substrain is susceptible to Listeria, all species of Leishmania, and several species of Trypanosoma, but is resistant to experimental allergic orchitis (EAO). |
The Jackson Laboratory
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Animal
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Mouse
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C57BL/6 mouse (Asokan study)
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Unspecified
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Animal
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Mouse
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C57BL/6J mouse
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C57BL/6J WT mouse |
The Jackson Laboratory
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Cell
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Human
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Primary cells
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CD4/CD8 Human Primary T cell
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CD4/CD8 Human Primary T cell |
Key Biologicals
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Cell
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Human
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Immortalized
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HEK-293-TgEF1a-eGFP-BSD
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HEK293 cells with lentiviral insertion of EF1a promoter driving expression of eGFP and SV40 promoter driving expression of BSD. HEK293 is an epithelial-like cell that was isolated from the kidney of a patient.
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Cell
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Human
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Immortalized
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HEK-293T with Ai9 transient reporter assay
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HEK-293T cells transfected with an Ai9 inducible transgene reporter plasmid used to test gene editing activity by fluorescence. HEK293T is an epithelial-like cell that was isolated from the kidney of a patient. |
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Cell
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Human
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Immortalized
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HEK-293T-disrupted_GFP with MCV-mcherry-Puro
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HEK293T cells with an integrated reporter for TLR-MCV1 reporter editing. HEK293T is an epithelial-like cell that was isolated from the kidney of a patient. |
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Cell
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Human
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Immortalized
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HEK-293T-disrupted_GFP-mcherry-Puro
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HEK293T cells with an integrated reporter for TLR1 reporter editing. HEK293T is an epithelial-like cell that was isolated from the kidney of a patient. |
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Cell
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Human
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HeLa
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HeLa cells were isolated in 1951 from a cervical carcinoma; Epithelial cell; Uterus; Cervix |
ATCC
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Cell
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Mouse
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Hepatoma
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Hepa1-6
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Stable mouse cell line of liver epithelial cells. |
ATCC
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Organoid
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Human
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Human kidney organoid (ESC-derived)
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Kidney organoid derived from human female H9 embryonic stem cells |
Morizane lab
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Organoid
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Human
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Human kidney organoid (iPSC-derived)
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Kidney organoid derived from human male BJFF iPS cells |
Morizane lab
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Organoid
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Human
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Human kidney organoid (stem cell-derived)
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Kidney organoid derived from human female H9 ES or BJFF iPS cells |
Morizane lab
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Cell
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Human
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K562
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Animal
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Rhesus macaque
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Macaca mulatta (Rhesus Macaque)
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Tarantal Lab
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Cell
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Mouse
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Primary cells
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Mouse Embryonic Fibroblasts
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Primary cell line |
In-house:
https://jacks-lab.mit.edu/protocols/making_mefs
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Cell
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Human
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Primary cells
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NK
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Natural Killer cells. White blood cells; |
GreenCross LabCell
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Cell
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Mouse
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Neuroblastoma
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Neuro 2A
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Neuro-2a cells are mouse neuroblasts with neuronal and amoeboid stem cell morphology isolated from brain tissue. |
ATCC
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Cell
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Human
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Primary cells
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Primary Airway Epithelia (Human)
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Primary Airway Epithelia cultured at the air liquid interface |
Cystic Fibrosis Foundation or McGill University
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Cell
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Human
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Primary cells
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Primary Airway Epithelia (Human)
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Primary airway epithelia from non-CF donors |
University of Iowa
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Cell
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Rhesus macaque
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Primary cells
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Primary Airway Epithelia (Rhesus Macaque)
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Rhesus macaque airway epithelial cells |
Tarantal Lab
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Cell
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Rhesus macaque
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Primary cells
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Primary skin fibroblasts (Rhesus Macaque)
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Rhesus macaque primary skin fibroblasts |
Tarantal Lab
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Animal
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Mouse
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TLR-MCV1 mouse
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TLR-MCV1 transgene knocked into Rosa26 locus |
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Animal
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Mouse
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mTmG mouse
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ROSAmT/mGÂ is a cell membrane-targeted, two-color fluorescent Cre-reporter allele. Prior to Cre recombination, cell membrane-localized tdTomato (mT) fluorescence expression is widespread in cells/tissues. Cre recombinase expressing cells (and future cell lineages derived from these cells) have cell membrane-localized EGFP (mG) fluorescence expression replacing the red fluorescence |
The Jackson Laboratory
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Animal
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Mouse
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mTmG mouse (congenic)
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mTmG is a double-fluorescent reporter transgenic mouse which expresses membrane-targeted tdTomato flanked by loxP sequences, followed by membrane-targeted GFP. After genomic cleavage by Cas9 at two sites, or Cre recombinase between loxP sites, tdTomato expression is lost and GFP is expressed. |
The Jackson Laboratory
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