Model System |
Animal
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Ai14 mouse
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Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain. |
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Model System |
Animal
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Ai14 mouse (congenic)
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Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain. |
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Model System |
Cell
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Ai14 mouse primary fibroblasts
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Primary fibroblasts from Ai14 mice |
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Model System |
Animal
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Ai9 mouse
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Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. |
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Model System |
Animal
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Ai9 mouse (BCM)
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Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. |
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Model System |
Cell
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Ai9 mouse immortalized fibroblasts
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Immortalized fibroblasts made from Ai9 (B6.Cg-Gt(ROSA)26Sor^tm9(CAG-tdTomato)Hze/J) mice |
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Model System |
Animal
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Ai9-SauSpyCas9 mouse
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Ai9-SauSpyCas9 is a version of Ai9 that has a single guide RNA sites on both sides of the STOP cassette. This is a Cre reporter allele that has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. Ai9 mice express robust tdTomato fluorescence following Cre-mediated recombination. |
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Model System |
Animal
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BALB/c mouse
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BALB/cJ is a commonly used inbred. Key traits include a susceptibility to developing the demyelinating disease upon infection with Theiler's murine encephalomyelitis virus. The BALB/cJ substrain is susceptible to Listeria, all species of Leishmania, and several species of Trypanosoma, but is resistant to experimental allergic orchitis (EAO). |
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Model System |
Animal
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C57BL/6 mouse (Asokan study)
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Model System |
Animal
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C57BL/6J mouse
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C57BL/6J WT mouse |
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Model System |
Cell
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CD4/CD8 Human Primary T cell
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CD4/CD8 Human Primary T cell |
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Model System |
Cell
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HEK-293-TgEF1a-eGFP-BSD
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HEK293 cells with lentiviral insertion of EF1a promoter driving expression of eGFP and SV40 promoter driving expression of BSD
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Model System |
Cell
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HEK-293T with Ai9 transient reporter assay
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HEK-293T cells transfected with an Ai9 inducible transgene reporter plasmid used to test gene editing activity by fluorescence |
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Model System |
Cell
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HEK-293T-disrupted_GFP with MCV-mcherry-Puro
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HEK293T cells with an integrated reporter for TLR-MCV1 reporter editing |
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Model System |
Cell
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HEK-293T-disrupted_GFP-mcherry-Puro
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HEK293T cells with an integrated reporter for TLR1 reporter editing |
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Model System |
Cell
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Hepa1-6
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Stable cell line |
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Model System |
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K562
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Model System |
Cell
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Mouse Embryonic Fibroblasts
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Primary cell line |
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Model System |
Cell
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NK
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Model System |
Cell
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Neuro 2A
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Stable cell line |
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Model System |
Cell
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Primary Airway Epithelia
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Primary airway epithelia from non-CF donors |
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Model System |
Animal
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TLR-MCV1
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TLR-MCV1 transgene knocked into Rosa26 locus |
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Model System |
Animal
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mTmG mouse
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ROSAmT/mG is a cell membrane-targeted, two-color fluorescent Cre-reporter allele. Prior to Cre recombination, cell membrane-localized tdTomato (mT) fluorescence expression is widespread in cells/tissues. Cre recombinase expressing cells (and future cell lineages derived from these cells) have cell membrane-localized EGFP (mG) fluorescence expression replacing the red fluorescence |
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Model System |
Animal
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mTmG mouse (congenic)
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mTmG is a double-fluorescent reporter transgenic mouse which expresses membrane-targeted tdTomato flanked by loxP sequences, followed by membrane-targeted GFP. After genomic cleavage by Cas9 at two sites, or Cre recombinase between loxP sites, tdTomato expression is lost and GFP is expressed. |
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