Model Systems
Model Systems are cells and animals of particular species (e.g., mouse, pig, human cultured cells) that substitute for humans in research for developing and understanding safe approaches to genome editing. Below is information about cell and animal models used and developed by SCGE consortium projects.
43 results in Model Systems
Ai14 mouseModel System - [In Vivo] [Delivery Systems] [Mouse] |
Ai14 mouse (congenic)Model System - [In Vivo] [Delivery Systems, Animal Reporter and Testing Center] [Mouse]Show Experiments (4)
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Ai14 mouse primary fibroblastsModel System - [In Vitro] [Delivery Systems] [Mouse] |
Ai9 mouseModel System - [In Vivo] [Delivery Systems, AAV tropism, Animal Reporter and Testing Center] [Mouse]Show Experiments (11)
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Ai9 mouse (BCM)Model System - [In Vivo] [Animal Reporter and Testing Center] [Mouse] |
Ai9 mouse immortalized fibroblastsModel System - [In Vitro] [Delivery Systems] [Mouse] |
Ai9-SauSpyCas9 mouseModel System - [In Vivo] [Animal Reporter and Testing Center] [Mouse] |
BALB/c mouseModel System - [In Vivo] [Delivery Systems] [Mouse]Show Experiments (1) |
C57BL/6 mouse (Asokan study)Model System - [In Vivo] [Delivery Systems] [Mouse] |
C57BL/6J mouseModel System - [In Vivo] [Delivery Systems] [Mouse]Show Experiments (3) |
CD4/CD8 Human Primary T cellModel System - [In Vitro] [Biological Effects] [Human]Show Experiments (1) |
GER10 Heterozygous BlastocystModel System - [In Vitro] [Animal Reporter and Testing Center] [Mouse]Show Experiments (1) |
GER12 Heterozygous BlastocystModel System - [In Vitro] [Animal Reporter and Testing Center] [Mouse]Show Experiments (1) |
GER14 Heterozygous BlastocystModel System - [In Vitro] [Animal Reporter and Testing Center] [Mouse]Show Experiments (1) |
GER18 Heterozygous BlastocystModel System - [In Vitro] [Animal Reporter and Testing Center] [Mouse]Show Experiments (1) |
GER19 Heterozygous BlastocystModel System - [In Vitro] [Animal Reporter and Testing Center] [Mouse]Show Experiments (1) |
HEK-293-TgEF1a-eGFP-BSDModel System - [In Vitro] [Genome Editors] [Human]Show Experiments (1) |
HEK-293T with Ai9 transient reporter assayModel System - [In Vitro] [Delivery Systems] [Human]Show Experiments (1) |
HEK-293T-disrupted_GFP with MCV-mcherry-PuroModel System - [In Vitro] [Delivery Systems] [Human] |
HEK-293T-disrupted_GFP-mcherry-PuroModel System - [In Vitro] [Delivery Systems] [Human] |
HeLaModel System - [In Vitro] [Delivery Systems, Genome Editors, Collaborative Opportunity Fund] [Human] |
Hepa1-6Model System - [In Vitro] [Delivery Systems] [Mouse]Show Experiments (1) |
Human kidney organoid (ESC-derived)Model System - [In Vitro] [Biological Effects] [Human] |
Human kidney organoid (iPSC-derived)Model System - [In Vitro] [Biological Effects] [Human] |
Human kidney organoid (stem cell-derived)Model System - [In Vitro] [Biological Effects] [Human]Show Experiments (4)
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K562Model System [Human] |
Liver On Chip (LoC)Model System - [In Vitro] [Delivery Systems, Collaborative Opportunity Fund, Biological Effects] [Human]Show Experiments (3)
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Macaca mulatta (Rhesus Macaque)Model System - [In Vivo] [Delivery Systems, Genome Editors, Collaborative Opportunity Fund] [Rhesus macaque]Show Experiments (1) |
NKModel System - [In Vitro] [Delivery Systems] [Human]Show Experiments (1) |
Neuro 2AModel System - [In Vitro] [Delivery Systems] [Mouse]Show Experiments (1) |
Primary Airway Epithelia (Human)Model System - [In Vitro] [Delivery Systems, Genome Editors, Collaborative Opportunity Fund] [Human]Show Experiments (4)
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Primary Airway Epithelia (Human)Model System - [In Vitro] [Delivery Systems, Genome Editors, Collaborative Opportunity Fund] [Human] |
Primary Airway Epithelia (Rhesus Macaque)Model System - [In Vitro] [Delivery Systems, Genome Editors, Collaborative Opportunity Fund] [Rhesus macaque] |
Primary skin fibroblasts (Rhesus Macaque)Model System - [In Vitro] [Delivery Systems, Genome Editors, Collaborative Opportunity Fund] [Rhesus macaque] |
TLR-2 mouse heterozygous blastocystModel System - [In Vitro] [Animal Reporter and Testing Center] [Mouse] |
TLR-2 mouse homozygous blastocystModel System - [In Vitro] [Animal Reporter and Testing Center] [Mouse] |
TLR-7 mouse heterozygous blastocystModel System - [In Vitro] [Animal Reporter and Testing Center] [Mouse] |
TLR-MCV1 mouseModel System - [In Vivo] [Delivery Systems] [Mouse] |
human kidney organoid with Ai9 (tdTomato) fluorescence-on reporterModel System - [In Vitro] [Delivery Systems, Collaborative Opportunity Fund, Biological Effects] [Human]Show Experiments (1) |
mTmG mouseModel System - [In Vivo] [Delivery Systems] [Mouse] |
mTmG mouse (congenic)Model System - [In Vivo] [Delivery Systems, Animal Reporter and Testing Center] [Mouse]Show Experiments (7)
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43 results in Model Systems
Type | Organism | Subtype | Name | Description | Source | View Associated... |
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Animal | Mouse | Ai14 mouse | Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain. | The Jackson Laboratory | ||
Animal | Mouse | Ai14 mouse (congenic) | Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain. | The Jackson Laboratory |
Show Experiments (4)
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Cell | Mouse | Primary cells | Ai14 mouse primary fibroblasts | Primary fibroblasts from Ai14 mice | ||
Animal | Mouse | Ai9 mouse | Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. | The Jackson Laboratory |
Show Experiments (11)
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Animal | Mouse | Ai9 mouse (BCM) | Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. | Baylor College of Medicine | ||
Cell | Mouse | Immortalized Fibroblast (unspecified) | Ai9 mouse immortalized fibroblasts | Immortalized fibroblasts made from Ai9 (B6.Cg-Gt(ROSA)26Sor^tm9(CAG-tdTomato)Hze/J) mice | ||
Animal | Mouse | Ai9-SauSpyCas9 mouse | Ai9-SauSpyCas9 is a version of Ai9 that has a single guide RNA sites on both sides of the STOP cassette. This is a Cre reporter allele that has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. Ai9 mice express robust tdTomato fluorescence following Cre-mediated recombination. | Baylor College of Medicine | ||
Animal | Mouse | B6 | C57BL/6J WT mouse | Baylor College of Medicine | ||
Animal | Mouse | BALB/c mouse | BALB/cJ is a commonly used inbred. Key traits include a susceptibility to developing the demyelinating disease upon infection with Theiler's murine encephalomyelitis virus. The BALB/cJ substrain is susceptible to Listeria, all species of Leishmania, and several species of Trypanosoma, but is resistant to experimental allergic orchitis (EAO). | The Jackson Laboratory |
Show Experiments (1) |
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Animal | Mouse | C57BL/6 mouse (Asokan study) | Unspecified | |||
Animal | Mouse | C57BL/6J mouse | C57BL/6J WT mouse | The Jackson Laboratory |
Show Experiments (3) |
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Cell | Human | Primary cells | CD4/CD8 Human Primary T cell | CD4/CD8 Human Primary T cell | Key Biologicals |
Show Experiments (1) |
Cell | Mouse | Primary cells | GER10 Heterozygous Blastocyst | Blastocyst derived from GER10 heterozygous mice | The Jackson Laboratory |
Show Experiments (1) |
Cell | Mouse | Primary cells | GER12 Heterozygous Blastocyst | Blastocyst derived from GER12 heterozygous mice | The Jackson Laboratory |
Show Experiments (1) |
Cell | Mouse | Primary cells | GER14 Heterozygous Blastocyst | Blastocyst derived from GER14 heterozygous mice | The Jackson Laboratory |
Show Experiments (1) |
Cell | Mouse | Primary cells | GER18 Heterozygous Blastocyst | Blastocyst derived from GER18 heterozygous mice | The Jackson Laboratory |
Show Experiments (1) |
Cell | Mouse | Primary cells | GER19 Heterozygous Blastocyst | Blastocyst derived from GER19 heterozygous mice | The Jackson Laboratory |
Show Experiments (1) |
Cell | Human | Immortalized | HEK-293-TgEF1a-eGFP-BSD | HEK293 cells with lentiviral insertion of EF1a promoter driving expression of eGFP and SV40 promoter driving expression of BSD. HEK293 is an epithelial-like cell that was isolated from the kidney of a patient. |
Show Experiments (1) |
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Cell | Human | Immortalized | HEK-293T with Ai9 transient reporter assay | HEK-293T cells transfected with an Ai9 inducible transgene reporter plasmid used to test gene editing activity by fluorescence. HEK293T is an epithelial-like cell that was isolated from the kidney of a patient. |
Show Experiments (1) |
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Cell | Human | Immortalized | HEK-293T-disrupted_GFP with MCV-mcherry-Puro | HEK293T cells with an integrated reporter for TLR-MCV1 reporter editing. HEK293T is an epithelial-like cell that was isolated from the kidney of a patient. | ||
Cell | Human | Immortalized | HEK-293T-disrupted_GFP-mcherry-Puro | HEK293T cells with an integrated reporter for TLR1 reporter editing. HEK293T is an epithelial-like cell that was isolated from the kidney of a patient. | ||
Cell | Human | HeLa | HeLa cells were isolated in 1951 from a cervical carcinoma; Epithelial cell; Uterus; Cervix | ATCC | ||
Cell | Mouse | Hepatoma | Hepa1-6 | Stable mouse cell line of liver epithelial cells. | ATCC |
Show Experiments (1) |
Organoid | Human | Human kidney organoid (ESC-derived) | Kidney organoid derived from human female H9 embryonic stem cells | Morizane lab | ||
Organoid | Human | Human kidney organoid (iPSC-derived) | Kidney organoid derived from human male BJFF iPS cells | Morizane lab | ||
Organoid | Human | Human kidney organoid (stem cell-derived) | Kidney organoid derived from human female H9 ES or BJFF iPS cells | Morizane lab |
Show Experiments (4)
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Cell | Human | K562 | ||||
Organoid | Human | Liver On Chip (LoC) | organoids derived from primary human hepatocytes |
Show Experiments (3)
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Animal | Rhesus macaque | Macaca mulatta (Rhesus Macaque) | Tarantal Lab |
Show Experiments (1) |
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Cell | Mouse | Primary cells | Mouse Embryonic Fibroblasts | Primary cell line | In-house: https://jacks-lab.mit.edu/protocols/making_mefs | |
Cell | Human | Primary cells | NK | Natural Killer cells. White blood cells; | GreenCross LabCell |
Show Experiments (1) |
Cell | Mouse | Neuroblastoma | Neuro 2A | Neuro-2a cells are mouse neuroblasts with neuronal and amoeboid stem cell morphology isolated from brain tissue. | ATCC |
Show Experiments (1) |
Cell | Human | Primary cells | Primary Airway Epithelia (Human) | Primary airway epithelia from non-CF donors | University of Iowa |
Show Experiments (4)
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Cell | Human | Primary cells | Primary Airway Epithelia (Human) | Primary Airway Epithelia cultured at the air liquid interface | Cystic Fibrosis Foundation or McGill University | |
Cell | Rhesus macaque | Primary cells | Primary Airway Epithelia (Rhesus Macaque) | Rhesus macaque airway epithelial cells | Tarantal Lab | |
Cell | Rhesus macaque | Primary cells | Primary skin fibroblasts (Rhesus Macaque) | Rhesus macaque primary skin fibroblasts | Tarantal Lab | |
Cell | Mouse | Primary cells | TLR-2 mouse heterozygous blastocyst | Blastocyst derived from TLR2 heterozygous mice | The Jackson Laboratory | |
Cell | Mouse | Primary cells | TLR-2 mouse homozygous blastocyst | Blastocyst derived from TLR2 homozygous mice | The Jackson Laboratory | |
Cell | Mouse | Primary cells | TLR-7 mouse heterozygous blastocyst | Blastocyst derived from TLR7 heterozygous mice | The Jackson Laboratory | |
Animal | Mouse | TLR-MCV1 mouse | TLR-MCV1 transgene knocked into Rosa26 locus | |||
Organoid | Human | human kidney organoid with Ai9 (tdTomato) fluorescence-on reporter | Kidney organoids were derived from a human iPS cell line with Ai9 (tdTomato) fluorescence-on reporter knocked into the AAVS1 safe harbor locus. | Beno Freedman Lab |
Show Experiments (1) |
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Animal | Mouse | mTmG mouse | ROSAmT/mGÂ is a cell membrane-targeted, two-color fluorescent Cre-reporter allele. Prior to Cre recombination, cell membrane-localized tdTomato (mT) fluorescence expression is widespread in cells/tissues. Cre recombinase expressing cells (and future cell lineages derived from these cells) have cell membrane-localized EGFP (mG) fluorescence expression replacing the red fluorescence | The Jackson Laboratory | ||
Animal | Mouse | mTmG mouse (congenic) | mTmG is a double-fluorescent reporter transgenic mouse which expresses membrane-targeted tdTomato flanked by loxP sequences, followed by membrane-targeted GFP. After genomic cleavage by Cas9 at two sites, or Cre recombinase between loxP sites, tdTomato expression is lost and GFP is expressed. | The Jackson Laboratory |
Show Experiments (7)
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