283 results for crispr

Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.   - Experiment [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain  -->  Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.  -->  Nanocapusules carrying CRISPR Cas9 RNP with guide RNA targeting the stop sequence in the Ai14 transgene  -->  Nanocapusules carrying CRISPR Cas9 RNP with guide RNA targeting the stop sequence in the Ai14 transgene name -->  Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.  -->  Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
Gong Shaoqin (Sarah)
Nanocapusules carrying CRISPR Cas9 RNP with guide RNA targeting the stop sequence in the Ai14 transgene are intracerebrally delivered to Ai14 mice and gene editing is measured by gain of tdTomato protein expression.
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors   - Project [Delivery Systems Initiative]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors  -->  RNA-guided CRISPR genome editing systems promise to revolutionize the treatment of inherited disease.  that directs editing is a critical hurdle in the development of clinical applications for engineered CRISPR  of nucleic acid therapeutics, we have established a framework for complete chemical modification of CRISPR name -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J NIH Report
RNA-guided CRISPR genome editing systems promise to revolutionize the treatment of inherited disease. Safe, effective, and target-tissue-specific delivery of the guide RNA that directs editing is a critical hurdle in the development of clinical applications for engineered CRISPR systems. Using strategies validated for the delivery of other categories of nucleic acid therapeutics, we have established a framework for complete chemical modification of CRISPR guides, thereby conferring in vivo stability and effective biodistribution properties. The proposed research will optimize these guides, as well as other editing components, for clinical use.
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides   - Project [Delivery Systems Initiative]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides name -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B NIH Report
The proposed research is relevant to the public health because genetic and acquired diseases affecting the airways pose major disease and economic burdens. By advancing the delivery of gene editing tools, it may be possible to therapeutically modify the cells lining the airways. This novel strategy has implications for the treatment of both monogenetic and acquired lungs disease, and may have applications for other somatic cell therapies.
Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain   - Project [Delivery Systems Initiative]
Matched on:  -->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain  -->  In vivo genome editing using CRISPR/Cas9 is anticipated to be the next wave of therapeutics for various name -->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain
Gong Shaoqin (Sarah) NIH Report
In vivo genome editing using CRISPR/Cas9 is anticipated to be the next wave of therapeutics for various major health threats, including neurodegenerative diseases. However, to date, very few Cas9-gRNA ribonucleoprotein in vivo delivery methods have been reported, and delivery to the brain has been particularly challenging. The unique nanocapsules we plan to develop will ultimately enable high efficiency neuron-targeted genome editing in the brain, thereby offering new hope to treat devastating neurodegenerative diseases.
[Validation] Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain   - Experiment [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to  -->  Delivery of CRISPR/Cas9 via RNP-loaded nanocages to the brain in Ai14 mice  -->  Delivery of CRISPR/Cas9 via RNP-loaded nanocages to the brain in Ai14 mice..SpCas9 with N- and C-terminal name -->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to  -->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Murray Stephen A
Delivery of CRISPR/Cas9 via RNP-loaded nanocages to the brain in Ai14 mice
Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems   - Project [Genome Editors]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems  -->  Expanding genome editing tools through exploration of new CRISPR-Cas proteins and DNA repair enzymes  NARRATIVE Fundamental research on bacterial adaptive immunity uncovered the genome editing properties of CRISPR-Cas  We will focus our investigation on newly described CRISPR-Cas systems and DNA-interacting proteins that name -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Banfield Jillian , Doudna Jennifer A NIH Report
Expanding genome editing tools through exploration of new CRISPR-Cas proteins and DNA repair enzymes NARRATIVE Fundamental research on bacterial adaptive immunity uncovered the genome editing properties of CRISPR-Cas systems, and it is clear that uncultivated microbes contain more pathways and enzymes that may be useful as genome editing tools. We will combine bioinformatics and biochemistry to identify new DNA- and RNA-associating proteins and will analyze their mechanisms of action. We will focus our investigation on newly described CRISPR-Cas systems and DNA-interacting proteins that occur in conserved genomic context.
[Validation] Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides   - Experiment [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using  -->  Ribonucleoproteins for CRISPR/Cas9 editing are complexed with amphiphilic peptides for delivery to lung  -->  Ribonucleoproteins for CRISPR/Cas9 editing are complexed with amphiphilic peptides for delivery to lung  transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated  transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated name -->  Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using  -->  Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using
Heaney Jason D
Ribonucleoproteins for CRISPR/Cas9 editing are complexed with amphiphilic peptides for delivery to lung airway epithilia via intranasal instillation into mTmG reporter mice. Editing is detected by production of GFP protein, and green fluorescence in airway linings
[Validation] Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear   - Experiment [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear  -->  Delivery of CRISPR/Cas9 via bioreducible lipid nanoparticles (LNPs) to the inner ear in Ai14 mice  -->  Delivery of CRISPR/Cas9 via bioreducible lipid nanoparticles (LNPs) to the inner ear in Ai14 mice ..SpCas9 name -->  Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear  -->  Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
Murray Stephen A
Delivery of CRISPR/Cas9 via bioreducible lipid nanoparticles (LNPs) to the inner ear in Ai14 mice
[Validation] Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear   - Experiment [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9  -->  Delivery of CRISPR/Cas9 editor via bioreducible lipid nanoparticle to the inner ear in Ai14 mice  -->  Delivery of CRISPR/Cas9 editor via bioreducible lipid nanoparticle to the inner ear in Ai14 mice ..SpCas9 name -->  Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9  -->  Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9
Murray Stephen A
Delivery of CRISPR/Cas9 editor via bioreducible lipid nanoparticle to the inner ear in Ai14 mice
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter)   - Experiment [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to name -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to
Asokan Aravind
A dual vector strategy was employed: one delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (both single stranded AAV cassettes). This strategy was evaluted with both AAV9 (n=3) and AAVcc47 (n=3) by intravenous injection in Ai9 mice. A total dose of 3e12vg was injected into each mouse (1.5e12vg each vector mixed 1:1) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter)   - Experiment [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 name -->  Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9  -->  Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
Asokan Aravind
A dual vector strategy was employed: one delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (both single stranded AAV cassettes). This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice. A total dose of 4e12vg was injected into each mouse and vectors mixed in a 1:4 ratio of cas9 to guide RNA (1e12vg of CMV Sacas9 vector and 3e12vg of the sgRNA vector) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter)   - Experiment [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to name -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
Asokan Aravind
A dual vector strategy was employed: one delivering a single guide RNA and CB driven SaCas9, and another delivering the second guide RNA and CB driven SaCas9. This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice. A total dose of 2e12vg was injected into each mouse (1e12vg each vector mixed 1:1) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.   - Experiment [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to name -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
Asokan Aravind
A dual vector strategy was employed: one self complementary vector delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (single stranded vector). This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=4) by intravenous injection in Ai9 mice. A total dose of 4e12vg was injected into each mouse and vectors mixed in a 1:1 ratio of cas9 to guide RNA (2e12vg of CMV Sacas9 vector and 2e12vg of the self complementary sgRNA vector) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
STS159 (mTmG; Sp_t2:Sp_c20_mTmG)   - Guide [Delivery Systems Initiative] - [In Vivo, In Vitro]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors  -->  transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated  cell line..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Targets mTmG and related reporter transgenes
D237   - Delivery System [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated  -->  Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using
SpyCas9 g-loxP2_C9   - Guide [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated  -->  Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using
This sgRNA targets the mTmG transgene
Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain   - Experiment [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors  -->  transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated  transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged.
D10   - Delivery System [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated  -->  Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using
STS204 (DNMT1; Sp_t2:Sp_c20_Dnmt1)   - Guide [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors  -->  locus......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
For endogenous locus
STS159 (mTmG; Sp_t2:Sp_c0_mTmG)   - Guide [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors  -->  epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Targets mTmG and related reporter transgenes
STS159 (mTmG; Sp_t2-PS:Sp_c0_mTmG)   - Guide [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors  -->  transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Targets mTmG and related reporter transgenes
STS159 (mTmG; Sp_t2-PS:Sp_c20_mTmG)   - Guide [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors  -->  transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Targets mTmG and related reporter transgenes
[Validation] Validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing.   - Experiment [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery.  -->  Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery.
Heaney Jason D
Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery. Detection of editing in non-target tissues.
AB_2532994    - Antibody [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Other Id: 13-0300  
Thermo-Fisher, Rat anti-GFAP
AB_141637    - Antibody [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Other Id: A21207 
Invitrogen, Donkey anti-rabbit alexa fluor 594
RNP-NC-no ligand   - Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain  -->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to  Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA.
Ai14 gRNA   - Guide [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain  -->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to  Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
This sgRNA targets the Ai9 and related transgenes at multiple sites
306-O12B blank   - Delivery System [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9
Combinatorial library cationic lipid nanoparticles
sNLS-SpCas9-sNLS   - Genome Editor  [Small Animal Testing Center (SATC), Delivery Systems Initiative]
Matched on:  -->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain  -->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to  Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
SpCas9 with N- and C-terminal SV40 NLS
RNP-NC-CPP   - Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain  -->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to  Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a cell penetrating peptide (CPP) from the TAT peptide (GRKKRRQRRRPQ) which lacks cell-type specficity
Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain   - Experiment [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors  -->  transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated  transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged.
STS159 (mTmG; Sp_t2:Sp_c20_mTmG)   - Guide [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors  -->  transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Targets mTmG and related reporter transgenes
CleanCap® Cas9 mRNA (5moU)   - Genome Editor  [Small Animal Testing Center (SATC)]
Matched on:  -->  Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9  Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
SpCas9 mRNA with 2 NLS signals, HA tag and capped using CleanCap. It is polyadenylated, substituted with a modified uridine and optimized for mammalian systems. It mimics a fully processed mature mRNA.
Testing newly chemically modified crRNA and tracrRNA in mTmG mouse embryonic fibroblasts   - Experiment [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors  -->  cell line..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated  cell line..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were delivered by electroporation to embryonic fibroblasts harvested from the mTmG reporter mouse. Gene editing was determined by reporter activation.
Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain   - Experiment [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors  -->  epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated  epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged.
AAVcc47-CMV-SaCas9   - Vector  [Delivery Systems Initiative]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to  Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
AAVcc47 delivering CMV driven SaCas9
Ai14 mouse   - Model System [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain  -->  Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain.
SpyCas9-3xNLS   - Genome Editor  [Delivery Systems Initiative]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors  -->  Rosa26 locus ..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
SpyCas9-3xNLS is type II-A Cas9 from Streptococcus pyogenes strain SF370. It was expressed from pMCSG7 bacterial expressing vector and purified from Escherichia coli Rosetta DE3 strain. SpyCas9 fused to 3 NLS: C-Myc-like NLS at the N-terminal SV40 NLS and Nucleoplasmin NLS at the C-terminal
sg298   - Guide [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9  Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
This sgRNA targets the Ai9 and related transgenes at multiple sites. 2'-O-Methyl at 3 first and last bases, 3' phosphorothioate bonds between first 3 and last 2 bases
SaCas9-Lagor   - Genome Editor  [Delivery Systems Initiative]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to  Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
RNP-NC-RVG   - Delivery System [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a RVG peptide YTIWMPENPRPGTPCDIFTNSRGKRASNG which specifically interacts withthe N-acetylecholine receptor (AchR) on neuronal cells, which mediates NP entry
AB_141607    - Antibody [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Other Id: A21202 
Invitrogen, Donkey anti-mouse alexafluor 488
AAV9-Ai9-sgRNA1 + sgRNA2   - Vector  [Delivery Systems Initiative]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to  Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus
SpCas9   - Genome Editor  [Small Animal Testing Center (SATC)]
Matched on:  -->  recombination...mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated  -->  Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using
AAV9-Ai9-sgRNA2-CB-SaCas9   - Vector  [Delivery Systems Initiative]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAV serotype 9 delivering gRNA 2 + CB SaCas9 targeting the Ai9 locus
Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain   - Experiment [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors  -->  transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated  transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged.
RRID:AB_2536526    - Antibody [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors  -->  transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Other Id: AB_2536526 
GFP Recombinant Rabbit Monoclonal Antibody, Thermo Fisher Scientific #G10362
AB_2813835    - Antibody [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Other Id: ab150155 
Abcam, Donkey anti-rat alexa fluour 647
AB_2209751    - Antibody [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Other Id: Rockland Cat# 600-401-379 
Anti-RFP (RABBIT) Antibody
AB_10711040    - Antibody [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Other Id: ab104224  
Abcam, mouse anti-NeuN
Mouse Embryonic Fibroblasts   - Model System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors  -->  cell line..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Primary cell line
Ai14 mouse (congenic)   - Model System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to  Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain.
Testing preparation for validation at The Jackson Laboratory Small Animal Testing Center   - Experiment [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors  -->  locus......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated  locus......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Sontheimer Erik J
Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
AAV9-CMV-SaCas9   - Vector  [Delivery Systems Initiative]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to  Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
AAV serotype 9 delivering CMV driven SaCas9
AAVcc47-Ai9-sgRNA1 + sgRNA2   - Vector  [Delivery Systems Initiative]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to  Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus
AAVcc47-Ai9-sgRNA1-CB-SaCas9   - Vector  [Delivery Systems Initiative]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAVcc47 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus
AAVcc47_pTR_self comp 2xU6-Ai9 guides   - Vector  [Delivery Systems Initiative]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAVcc47 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 transgene
AAV9-Ai9-sgRNA1-CB-SaCas9   - Vector  [Delivery Systems Initiative]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAV serotype 9 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus
AAVcc47-Ai9-sgRNA2-CB-SaCas9   - Vector  [Delivery Systems Initiative]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAVcc47 delivering sgRNA 2 + CB SaCas9 targeting the Ai9 locus
[Validation] Second site validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain   - Experiment [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  Validation of delivery of AAV custom designed to cross the blood-brain barrier for CRISPR/Cas9 editing  -->  Validation of delivery of AAV custom designed to cross the blood-brain barrier for CRISPR/Cas9 editing
Heaney Jason D
Validation of delivery of AAV custom designed to cross the blood-brain barrier for CRISPR/Cas9 editing. Editing detected and quantified in brain by generation of tdTomato fluorescent protein signal from Ai9 reporter mice
AAV9_pTR_self comp 2xU6-Ai9 guides   - Vector  [Delivery Systems Initiative]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 transgene
Ai9 mouse   - Model System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to  Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus.
306-O12B   - Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo, In Vitro]
Matched on:  -->  Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9  Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
Combinatorial library cationic lipid nanoparticles
RRID:AB_1196615    - Antibody [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors  -->  epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Other Id: AB_1196615 
GFP (D5.1) XP Rabbit mAb antibody, Cell Signaling Technology
[Validation] Validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice   - Experiment [Small Animal Testing Center (SATC)] - [In Vivo]
Matched on:  -->  AAV5 encoding CRISPR/Cas editing machinery were delivered to the lungs of reporter mice by intratracheal  -->  AAV5 encoding CRISPR/Cas editing machinery were delivered to the lungs of reporter mice by intratracheal
Heaney Jason D
AAV5 encoding CRISPR/Cas editing machinery were delivered to the lungs of reporter mice by intratracheal instillation. After 4 weeks incubation, the mice were dissected and the lungs imaged for the presence of tdTomato fluorescence, indicating successful editing. Editing calculated by dividing the number of tdTomato+ red cells by the number of nuclei in each airway
S10   - Delivery System [Delivery Systems Initiative] - [In Vivo, In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors  -->  epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Shuttle peptide used to deliver reagents to airway epithelia
306-S10   - Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo, In Vitro]
Matched on:  -->  Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
combinatorial library cationic lipid nanoparticles
SpyCas9-3xNLS   - Genome Editor  [Delivery Systems Initiative]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors  -->  epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated  cell line..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
SpyCas9-3xNLS is type II-A Cas9 from Streptococcus pyogenes strain SF370. It was expressed from pMCSG7 bacterial expressing vector and purified from Escherichia coli Rosetta DE3 strain. SpyCas9 fused to 3 NLS: C-Myc-like NLS at the N-terminal SV40 NLS and Nucleoplasmin NLS at the C-terminal
Testing Shuttle Peptides ability to deliver GFP-NLS to airway epithelia.   - Experiment [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B
Delivery of GFP via shuttle peptides to mouse airway epithelium via nasal instilation. Delivery efficiency was quantified in large and small airways by counting the number of GFP positive cells divided by the number of DAPI cells.
Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells.   - Experiment [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B
In Vitro shuttle peptide delivery of Cas12a RNPs targeting human CFTR and HPRT genes in human primary airway epithelia. Editing efficiency was assessed after 72hrs by sanger sequencing.
SpCas9 (Feldan Therapeutics)   - Genome Editor  [Delivery Systems Initiative]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
CM18-PTD4   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide
FS48   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD112   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD115d1   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD121   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD122   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD168   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD168d10   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD168d11   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD228   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD236   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD239   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD259   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD303   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD315   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD323   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD331   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD335   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD339   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD341   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD359   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD362   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD57   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD95   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSX7   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
g-loxPbot_C12a   - Guide [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
This sgRNA targets the Ai9 and related transgenes at two sites
Testing newly chemically modified crRNA and tracrRNA to activate the TLR reporter in human cells   - Experiment [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were delivered by electroporation in presence of amphiphilic peptide to transgenic human HEK-293T cells harboring the TLR-MCV1 reporter. Gene editing was determined by reporter activation.
TLR-MCV1   - Model System [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
TLR-MCV1 transgene knocked into Rosa26 locus
AsCas12a (IDT and Feldan Therapeutics)   - Genome Editor  [Delivery Systems Initiative]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
FS66d6   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD114d1   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD115   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD147   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD188   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD189   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD190   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD193   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD196   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD197   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD222   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD234   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD240   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD260   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD284   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD288   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD293   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD308   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD316   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD321   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD360   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD365   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD57d4   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD97   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
S10D   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
S18   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide
g-45_C12a   - Guide [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
gRNA targeting CFTR inton-22-23
Delivery of RNP containing chemically modified crRNA C20 with chemically modified tracrRNA T2-PS to determine the RNP distribution in TLR-MCV mouse brain   - Experiment [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were injected into the striatum of TLR-MCV mice and the distribution of RNP was imaged.
STS119 (TLR-MCV1b; Sp_t2:Sp_c20_TLR_MCV1b)   - Guide [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets traffic light reporter transgene
STS134 (PCSK9b; Sp_t2:Sp_c20_PCSK9b)   - Guide [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets endogenous mouse locus
STS135 (PCSK9c; Sp_t2:Sp_c20_PCSK9c)   - Guide [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets endogenous mouse locus
Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia   - Experiment [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B
In vivo shuttle peptide delivery of Cas9 and Cas12a RNPs in mouse airway epithelia. Gene editing was quantified by the GFP+ cells in large and small airways following 1 delivery of GFP protein by GFP positive cells compared to DAPI stained cells.
GFP-NLS   - Genome Editor  [Delivery Systems Initiative]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Nuclear targeted GFP
FSD114   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD116d1   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD118   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD132   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD168d12   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD195   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD199   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD215   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD237   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD262   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD285   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD286   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD297   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD310   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD319   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD322   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD332   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD364   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD366   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD63   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD67   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD94   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD96   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSX5   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
S10-MOD   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
S85   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide
mTmG mouse   - Model System [Delivery Systems Initiative] - [In Vivo]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
ROSAmT/mG is a cell membrane-targeted, two-color fluorescent Cre-reporter allele. Prior to Cre recombination, cell membrane-localized tdTomato (mT) fluorescence expression is widespread in cells/tissues. Cre recombinase expressing cells (and future cell lineages derived from these cells) have cell membrane-localized EGFP (mG) fluorescence expression replacing the red fluorescence
Testing newly discovered Biggie Phage editors in human cells   - Experiment [Genome Editors] - [In Vitro]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Banfield Jillian , Doudna Jennifer A
Cas12j-GFP23 (CasΦ-1)   - Guide [Genome Editors] - [In Vitro]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasΦ-1
Cas12j-GFP3 (CasΦ-2)   - Guide [Genome Editors] - [In Vitro]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasΦ-2
Cas12j-GFP8 (CasΦ-3)   - Guide [Genome Editors] - [In Vitro]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasΦ-3
Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells   - Experiment [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B
Delivery of Cas12a RNP targeting human CFTR in Primary human epithelia cells. Gene editing efficiency was determined by percentage of NGS reads that showed an indel
Alt-R® S.p. Cas9 Nuclease V3   - Genome Editor  [Delivery Systems Initiative]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
FSD168 Scr.   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD186   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD191   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD216   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD227   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD283   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD287   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD289   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD301   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD304   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD306   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD317   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD329   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD333   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD334   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD347   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD368   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD57d1   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
FSD57d5   - Delivery System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
3849 45-5'   - Guide [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Targets endogenous human locus
g-38330_C12a   - Guide [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
gRNA targeting HPRT locus
NKG2A   - Guide [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
gRNA targeting human NKG2A exon 3
STS120 (TLR1; Sp_t2:Sp_c20_TLR1)   - Guide [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets traffic light reporter transgene
Neuro 2A   - Model System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Stable cell line
Testing newly chemically modified crRNA and tracrRNA in mouse Hepa 1-6 cells   - Experiment [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were delivered by electroporation to mouse Hepa 1-6 cells. Editing activity was determined by Sanger sequencing
STS118 (TLR-MCV1a; Sp_t2:Sp_c20_TLR_MCV1a)   - Guide [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets traffic light reporter transgene
HEK-293T-disrupted_GFP with MCV-mcherry-Puro   - Model System [Delivery Systems Initiative] - [In Vitro]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
HEK293T cells with an integrated reporter for TLR-MCV1 reporter editing