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Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. - Experiment [Delivery Systems Initiative] - [In Vivo] Matched on: --> Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain --> Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. --> Nanocapusules carrying CRISPR Cas9 RNP with guide RNA targeting the stop sequence in the Ai14 transgene --> Nanocapusules carrying CRISPR Cas9 RNP with guide RNA targeting the stop sequence in the Ai14 transgene name --> Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. --> Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. Gong Shaoqin (Sarah) Nanocapusules carrying CRISPR Cas9 RNP with guide RNA targeting the stop sequence in the Ai14 transgene are intracerebrally delivered to Ai14 mice and gene editing is measured by gain of tdTomato protein expression. Projects Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors - Project [Delivery Systems Initiative] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors --> RNA-guided CRISPR genome editing systems promise to revolutionize the treatment of inherited disease. that directs editing is a critical hurdle in the development of clinical applications for engineered CRISPR of nucleic acid therapeutics, we have established a framework for complete chemical modification of CRISPR name --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Sontheimer Erik J RNA-guided CRISPR genome editing systems promise to revolutionize the treatment of inherited disease. Safe, effective, and target-tissue-specific delivery of the guide RNA that directs editing is a critical hurdle in the development of clinical applications for engineered CRISPR systems. Using strategies validated for the delivery of other categories of nucleic acid therapeutics, we have established a framework for complete chemical modification of CRISPR guides, thereby conferring in vivo stability and effective biodistribution properties. The proposed research will optimize these guides, as well as other editing components, for clinical use. Experiments Testing newly chemically modified crRNA and tracrRNA in mTmG mouse embryonic fibroblasts Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain Testing newly chemically modified crRNA and tracrRNA to activate the TLR1 reporter in human cells Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain Testing newly chemically modified crRNA and tracrRNA in mouse Hepa 1-6 cells Delivery of RNP containing chemically modified crRNA C20 with chemically modified tracrRNA T2-PS to determine the RNP distribution in TLR-MCV mouse brain Testing newly chemically modified crRNA and tracrRNA in mouse Neuro 2A cells Testing preparation for validation at The Jackson Laboratory Small Animal Testing Center Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain Testing newly chemically modified crRNA and tracrRNA to activate the TLR reporter in human cells Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides - Project [Delivery Systems Initiative] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides name --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides McCray Paul B The proposed research is relevant to the public health because genetic and acquired diseases affecting the airways pose major disease and economic burdens. By advancing the delivery of gene editing tools, it may be possible to therapeutically modify the cells lining the airways. This novel strategy has implications for the treatment of both monogenetic and acquired lungs disease, and may have applications for other somatic cell therapies. Experiments Testing Shuttle Peptides ability to deliver GFP-NLS to airway epithelia. Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells. Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells Gene editing in vitro by various peptide variants delivering Cas12a in NK cells.
Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain - Project [Delivery Systems Initiative] Matched on: --> Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain --> In vivo genome editing using CRISPR/Cas9 is anticipated to be the next wave of therapeutics for various name --> Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Gong Shaoqin (Sarah) In vivo genome editing using CRISPR/Cas9 is anticipated to be the next wave of therapeutics for various major health threats, including neurodegenerative diseases. However, to date, very few Cas9-gRNA ribonucleoprotein in vivo delivery methods have been reported, and delivery to the brain has been particularly challenging. The unique nanocapsules we plan to develop will ultimately enable high efficiency neuron-targeted genome editing in the brain, thereby offering new hope to treat devastating neurodegenerative diseases. Experiments Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
[Validation] Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain - Experiment [Small Animal Testing Center (SATC)] - [In Vivo] Matched on: --> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to --> Delivery of CRISPR/Cas9 via RNP-loaded nanocages to the brain in Ai14 mice --> Delivery of CRISPR/Cas9 via RNP-loaded nanocages to the brain in Ai14 mice..SpCas9 with N- and C-terminal name --> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to --> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to Murray Stephen A Delivery of CRISPR/Cas9 via RNP-loaded nanocages to the brain in Ai14 mice Projects The Jackson Laboratory Gene Editing Testing Center - Gong Validation (Gong, Shaoqin)
Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems - Project [Genome Editors] Matched on: --> Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems --> Expanding genome editing tools through exploration of new CRISPR-Cas proteins and DNA repair enzymes NARRATIVE Fundamental research on bacterial adaptive immunity uncovered the genome editing properties of CRISPR-Cas We will focus our investigation on newly described CRISPR-Cas systems and DNA-interacting proteins that name --> Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems Banfield Jillian , Doudna Jennifer A Expanding genome editing tools through exploration of new CRISPR-Cas proteins and DNA repair enzymes NARRATIVE Fundamental research on bacterial adaptive immunity uncovered the genome editing properties of CRISPR-Cas systems, and it is clear that uncultivated microbes contain more pathways and enzymes that may be useful as genome editing tools. We will combine bioinformatics and biochemistry to identify new DNA- and RNA-associating proteins and will analyze their mechanisms of action. We will focus our investigation on newly described CRISPR-Cas systems and DNA-interacting proteins that occur in conserved genomic context. Experiments Testing newly discovered Biggie Phage editors in human cells
[Validation] Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides - Experiment [Small Animal Testing Center (SATC)] - [In Vivo] Matched on: --> Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using --> Ribonucleoproteins for CRISPR/Cas9 editing are complexed with amphiphilic peptides for delivery to lung --> Ribonucleoproteins for CRISPR/Cas9 editing are complexed with amphiphilic peptides for delivery to lung transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated name --> Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using --> Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Heaney Jason D Ribonucleoproteins for CRISPR/Cas9 editing are complexed with amphiphilic peptides for delivery to lung airway epithilia via intranasal instillation into mTmG reporter mice. Editing is detected by production of GFP protein, and green fluorescence in airway linings Projects BCM-Rice Resource for the Analysis of Somatic Gene Editing in Mice - McCray Validation (McCray Jr., Paul)
[Validation] Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear - Experiment [Small Animal Testing Center (SATC)] - [In Vivo] Matched on: --> Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear --> Delivery of CRISPR/Cas9 via bioreducible lipid nanoparticles (LNPs) to the inner ear in Ai14 mice --> Delivery of CRISPR/Cas9 via bioreducible lipid nanoparticles (LNPs) to the inner ear in Ai14 mice ..SpCas9 name --> Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear --> Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Murray Stephen A Delivery of CRISPR/Cas9 via bioreducible lipid nanoparticles (LNPs) to the inner ear in Ai14 mice Projects The Jackson Laboratory Gene Editing Testing Center - Chen Validation (Chen, Zheng-Yi)
[Validation] Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear - Experiment [Small Animal Testing Center (SATC)] - [In Vivo] Matched on: --> Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 --> Delivery of CRISPR/Cas9 editor via bioreducible lipid nanoparticle to the inner ear in Ai14 mice --> Delivery of CRISPR/Cas9 editor via bioreducible lipid nanoparticle to the inner ear in Ai14 mice ..SpCas9 name --> Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 --> Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 Murray Stephen A Delivery of CRISPR/Cas9 editor via bioreducible lipid nanoparticle to the inner ear in Ai14 mice Projects The Jackson Laboratory Gene Editing Testing Center - Chen Validation (Chen, Zheng-Yi)
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) - Experiment [Delivery Systems Initiative] - [In Vivo] Matched on: --> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to name --> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to --> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to Asokan Aravind A dual vector strategy was employed: one delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (both single stranded AAV cassettes). This strategy was evaluted with both AAV9 (n=3) and AAVcc47 (n=3) by intravenous injection in Ai9 mice. A total dose of 3e12vg was injected into each mouse (1.5e12vg each vector mixed 1:1) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart. Projects Evolving High Potency AAV Vectors for Neuromuscular Genome Editing
Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter) - Experiment [Delivery Systems Initiative] - [In Vivo] Matched on: --> Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 name --> Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 --> Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 Asokan Aravind A dual vector strategy was employed: one delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (both single stranded AAV cassettes). This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice. A total dose of 4e12vg was injected into each mouse and vectors mixed in a 1:4 ratio of cas9 to guide RNA (1e12vg of CMV Sacas9 vector and 3e12vg of the sgRNA vector) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart. Projects Evolving High Potency AAV Vectors for Neuromuscular Genome Editing
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter) - Experiment [Delivery Systems Initiative] - [In Vivo] Matched on: --> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to name --> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to --> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to Asokan Aravind A dual vector strategy was employed: one delivering a single guide RNA and CB driven SaCas9, and another delivering the second guide RNA and CB driven SaCas9. This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice. A total dose of 2e12vg was injected into each mouse (1e12vg each vector mixed 1:1) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart. Projects Evolving High Potency AAV Vectors for Neuromuscular Genome Editing
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector. - Experiment [Delivery Systems Initiative] - [In Vivo] Matched on: --> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to name --> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to --> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to Asokan Aravind A dual vector strategy was employed: one self complementary vector delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (single stranded vector). This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=4) by intravenous injection in Ai9 mice. A total dose of 4e12vg was injected into each mouse and vectors mixed in a 1:1 ratio of cas9 to guide RNA (2e12vg of CMV Sacas9 vector and 2e12vg of the self complementary sgRNA vector) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart. Projects Evolving High Potency AAV Vectors for Neuromuscular Genome Editing
STS159 (mTmG; Sp_t2:Sp_c20_mTmG) - Guide [Delivery Systems Initiative] - [In Vivo, In Vitro] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors --> transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated cell line..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Targets mTmG and related reporter transgenes Experiments Testing newly chemically modified crRNA and tracrRNA in mTmG mouse embryonic fibroblasts Testing preparation for validation at The Jackson Laboratory Small Animal Testing Center
D237 - Delivery System [Small Animal Testing Center (SATC)] - [In Vivo] Matched on: --> transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated --> Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Experiments Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
SpyCas9 g-loxP2_C9 - Guide [Small Animal Testing Center (SATC)] - [In Vivo] Matched on: --> transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated --> Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using This sgRNA targets the mTmG transgene Experiments Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain - Experiment [Delivery Systems Initiative] - [In Vivo] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors --> transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Sontheimer Erik J Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged. Projects Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
D10 - Delivery System [Small Animal Testing Center (SATC)] - [In Vivo] Matched on: --> transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated --> Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Experiments Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
STS204 (DNMT1; Sp_t2:Sp_c20_Dnmt1) - Guide [Delivery Systems Initiative] - [In Vivo] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors --> locus......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated For endogenous locus Experiments Testing preparation for validation at The Jackson Laboratory Small Animal Testing Center
STS159 (mTmG; Sp_t2:Sp_c0_mTmG) - Guide [Delivery Systems Initiative] - [In Vivo] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors --> epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Targets mTmG and related reporter transgenes Experiments Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain
STS159 (mTmG; Sp_t2-PS:Sp_c0_mTmG) - Guide [Delivery Systems Initiative] - [In Vivo] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors --> transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Targets mTmG and related reporter transgenes Experiments Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
STS159 (mTmG; Sp_t2-PS:Sp_c20_mTmG) - Guide [Delivery Systems Initiative] - [In Vivo] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors --> transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Targets mTmG and related reporter transgenes Experiments Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
[Validation] Validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing. - Experiment [Small Animal Testing Center (SATC)] - [In Vivo] Matched on: --> Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery. --> Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery. Heaney Jason D Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery. Detection of editing in non-target tissues. Projects BCM-Rice Resource for the Analysis of Somatic Gene Editing in Mice - Asokan Validation (Asokan, Aravind)
AB_2532994 - Antibody [Small Animal Testing Center (SATC)] - [In Vivo] Matched on: --> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to Other Id: 13-0300 Thermo-Fisher, Rat anti-GFAP Experiments Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain
AB_141637 - Antibody [Small Animal Testing Center (SATC)] - [In Vivo] Matched on: --> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to Other Id: A21207 Invitrogen, Donkey anti-rabbit alexa fluor 594 Experiments Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain
RNP-NC-no ligand - Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo] Matched on: --> Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain --> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. Experiments Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
Ai14 gRNA - Guide [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo] Matched on: --> Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain --> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. This sgRNA targets the Ai9 and related transgenes at multiple sites Experiments Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
306-O12B blank - Delivery System [Small Animal Testing Center (SATC)] - [In Vivo] Matched on: --> Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 Combinatorial library cationic lipid nanoparticles Experiments Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
sNLS-SpCas9-sNLS - Genome Editor [Small Animal Testing Center (SATC), Delivery Systems Initiative] Matched on: --> Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain --> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. SpCas9 with N- and C-terminal SV40 NLS Experiments Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
RNP-NC-CPP - Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo] Matched on: --> Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain --> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a cell penetrating peptide (CPP) from the TAT peptide (GRKKRRQRRRPQ) which lacks cell-type specficity Experiments Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain - Experiment [Delivery Systems Initiative] - [In Vivo] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors --> transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Sontheimer Erik J Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged. Projects Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
STS159 (mTmG; Sp_t2:Sp_c20_mTmG) - Guide [Delivery Systems Initiative] - [In Vivo] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors --> transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Targets mTmG and related reporter transgenes Experiments Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
CleanCap® Cas9 mRNA (5moU) - Genome Editor [Small Animal Testing Center (SATC)] Matched on: --> Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear SpCas9 mRNA with 2 NLS signals, HA tag and capped using CleanCap. It is polyadenylated, substituted with a modified uridine and optimized for mammalian systems. It mimics a fully processed mature mRNA. Experiments Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
Testing newly chemically modified crRNA and tracrRNA in mTmG mouse embryonic fibroblasts - Experiment [Delivery Systems Initiative] - [In Vitro] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors --> cell line..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated cell line..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Sontheimer Erik J Chemically modified crRNA and tracrRNA were delivered by electroporation to embryonic fibroblasts harvested from the mTmG reporter mouse. Gene editing was determined by reporter activation. Projects Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain - Experiment [Delivery Systems Initiative] - [In Vivo] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors --> epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Sontheimer Erik J Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged. Projects Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
AAVcc47-CMV-SaCas9 - Vector [Delivery Systems Initiative] Matched on: --> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 AAVcc47 delivering CMV driven SaCas9 Experiments Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter)
Ai14 mouse - Model System [Delivery Systems Initiative] - [In Vivo] Matched on: --> Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain --> Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain. Experiments Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
SpyCas9-3xNLS - Genome Editor [Delivery Systems Initiative] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors --> Rosa26 locus ..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated SpyCas9-3xNLS is type II-A Cas9 from Streptococcus pyogenes strain SF370. It was expressed from pMCSG7 bacterial expressing vector and purified from Escherichia coli Rosetta DE3 strain. SpyCas9 fused to 3 NLS: C-Myc-like NLS at the N-terminal SV40 NLS and Nucleoplasmin NLS at the C-terminal Experiments Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain Delivery of RNP containing chemically modified crRNA C20 with chemically modified tracrRNA T2-PS to determine the RNP distribution in TLR-MCV mouse brain Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
sg298 - Guide [Small Animal Testing Center (SATC)] - [In Vivo] Matched on: --> Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear This sgRNA targets the Ai9 and related transgenes at multiple sites. 2'-O-Methyl at 3 first and last bases, 3' phosphorothioate bonds between first 3 and last 2 bases Experiments Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
SaCas9-Lagor - Genome Editor [Delivery Systems Initiative] Matched on: --> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 Experiments Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter) Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.
RNP-NC-RVG - Delivery System [Small Animal Testing Center (SATC)] - [In Vivo] Matched on: --> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a RVG peptide YTIWMPENPRPGTPCDIFTNSRGKRASNG which specifically interacts withthe N-acetylecholine receptor (AchR) on neuronal cells, which mediates NP entry Experiments Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain
AB_141607 - Antibody [Small Animal Testing Center (SATC)] - [In Vivo] Matched on: --> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to Other Id: A21202 Invitrogen, Donkey anti-mouse alexafluor 488 Experiments Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain
AAV9-Ai9-sgRNA1 + sgRNA2 - Vector [Delivery Systems Initiative] Matched on: --> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus Experiments Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter)
SpCas9 - Genome Editor [Small Animal Testing Center (SATC)] Matched on: --> recombination...mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated --> Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Experiments Validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
AAV9-Ai9-sgRNA2-CB-SaCas9 - Vector [Delivery Systems Initiative] Matched on: --> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to AAV serotype 9 delivering gRNA 2 + CB SaCas9 targeting the Ai9 locus Experiments Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter)
Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain - Experiment [Delivery Systems Initiative] - [In Vivo] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors --> transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Sontheimer Erik J Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged. Projects Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
RRID:AB_2536526 - Antibody [Delivery Systems Initiative] - [In Vivo] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors --> transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Other Id: AB_2536526 GFP Recombinant Rabbit Monoclonal Antibody, Thermo Fisher Scientific #G10362 Experiments Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
AB_2813835 - Antibody [Small Animal Testing Center (SATC)] - [In Vivo] Matched on: --> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to Other Id: ab150155 Abcam, Donkey anti-rat alexa fluour 647 Experiments Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain
AB_2209751 - Antibody [Small Animal Testing Center (SATC)] - [In Vivo] Matched on: --> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to Other Id: Rockland Cat# 600-401-379 Anti-RFP (RABBIT) Antibody Experiments Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain
AB_10711040 - Antibody [Small Animal Testing Center (SATC)] - [In Vivo] Matched on: --> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to Other Id: ab104224 Abcam, mouse anti-NeuN Experiments Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain
Mouse Embryonic Fibroblasts - Model System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors --> cell line..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Primary cell line Experiments Testing newly chemically modified crRNA and tracrRNA in mTmG mouse embryonic fibroblasts
Ai14 mouse (congenic) - Model System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo] Matched on: --> Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain. Experiments Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea
Testing preparation for validation at The Jackson Laboratory Small Animal Testing Center - Experiment [Delivery Systems Initiative] - [In Vivo] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors --> locus......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated locus......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Sontheimer Erik J Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain Projects Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
AAV9-CMV-SaCas9 - Vector [Delivery Systems Initiative] Matched on: --> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 AAV serotype 9 delivering CMV driven SaCas9 Experiments Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.
AAVcc47-Ai9-sgRNA1 + sgRNA2 - Vector [Delivery Systems Initiative] Matched on: --> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus Experiments Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter)
AAVcc47-Ai9-sgRNA1-CB-SaCas9 - Vector [Delivery Systems Initiative] Matched on: --> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to AAVcc47 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus Experiments Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter)
AAVcc47_pTR_self comp 2xU6-Ai9 guides - Vector [Delivery Systems Initiative] Matched on: --> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to AAVcc47 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 transgene Experiments Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.
AAV9-Ai9-sgRNA1-CB-SaCas9 - Vector [Delivery Systems Initiative] Matched on: --> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to AAV serotype 9 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus Experiments Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter)
AAVcc47-Ai9-sgRNA2-CB-SaCas9 - Vector [Delivery Systems Initiative] Matched on: --> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to AAVcc47 delivering sgRNA 2 + CB SaCas9 targeting the Ai9 locus Experiments Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter)
[Validation] Second site validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain - Experiment [Small Animal Testing Center (SATC)] - [In Vivo] Matched on: --> Validation of delivery of AAV custom designed to cross the blood-brain barrier for CRISPR/Cas9 editing --> Validation of delivery of AAV custom designed to cross the blood-brain barrier for CRISPR/Cas9 editing Heaney Jason D Validation of delivery of AAV custom designed to cross the blood-brain barrier for CRISPR/Cas9 editing. Editing detected and quantified in brain by generation of tdTomato fluorescent protein signal from Ai9 reporter mice Projects BCM-Rice Resource for the Analysis of Somatic Gene Editing in Mice - Deverman Validation (Deverman, Benjamin)
AAV9_pTR_self comp 2xU6-Ai9 guides - Vector [Delivery Systems Initiative] Matched on: --> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 transgene Experiments Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.
Ai9 mouse - Model System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo] Matched on: --> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. Experiments Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter) Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) Cre Recombinase dose escalation study in Ai9 mice Second site validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.
306-O12B - Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo, In Vitro] Matched on: --> Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Combinatorial library cationic lipid nanoparticles Experiments Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea Testing new LNPs (lipid nanoparticles) for delivery of Fluc mRNA in adult mice Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea
RRID:AB_1196615 - Antibody [Delivery Systems Initiative] - [In Vivo] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors --> epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Other Id: AB_1196615 GFP (D5.1) XP Rabbit mAb antibody, Cell Signaling Technology Experiments Testing preparation for validation at The Jackson Laboratory Small Animal Testing Center Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
[Validation] Validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice - Experiment [Small Animal Testing Center (SATC)] - [In Vivo] Matched on: --> AAV5 encoding CRISPR/Cas editing machinery were delivered to the lungs of reporter mice by intratracheal --> AAV5 encoding CRISPR/Cas editing machinery were delivered to the lungs of reporter mice by intratracheal Heaney Jason D AAV5 encoding CRISPR/Cas editing machinery were delivered to the lungs of reporter mice by intratracheal instillation. After 4 weeks incubation, the mice were dissected and the lungs imaged for the presence of tdTomato fluorescence, indicating successful editing. Editing calculated by dividing the number of tdTomato+ red cells by the number of nuclei in each airway Projects BCM-Rice Resource for the Analysis of Somatic Gene Editing in Mice - Gao Validation (Gao, Guang-Ping )
S10 - Delivery System [Delivery Systems Initiative] - [In Vivo, In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors --> epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Shuttle peptide used to deliver reagents to airway epithelia Experiments Testing Shuttle Peptides ability to deliver GFP-NLS to airway epithelia. Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells. Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells Gene editing in vitro by various peptide variants delivering Cas12a in NK cells.
306-S10 - Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo, In Vitro] Matched on: --> Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear combinatorial library cationic lipid nanoparticles Experiments Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea Testing new LNPs (lipid nanoparticles) for delivery of Fluc mRNA in adult mice Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea
SpyCas9-3xNLS - Genome Editor [Delivery Systems Initiative] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors --> epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated cell line..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated SpyCas9-3xNLS is type II-A Cas9 from Streptococcus pyogenes strain SF370. It was expressed from pMCSG7 bacterial expressing vector and purified from Escherichia coli Rosetta DE3 strain. SpyCas9 fused to 3 NLS: C-Myc-like NLS at the N-terminal SV40 NLS and Nucleoplasmin NLS at the C-terminal Experiments Testing newly chemically modified crRNA and tracrRNA in mTmG mouse embryonic fibroblasts Testing newly chemically modified crRNA and tracrRNA to activate the TLR1 reporter in human cells Testing newly chemically modified crRNA and tracrRNA in mouse Hepa 1-6 cells Testing newly chemically modified crRNA and tracrRNA in mouse Neuro 2A cells Testing preparation for validation at The Jackson Laboratory Small Animal Testing Center Testing newly chemically modified crRNA and tracrRNA to activate the TLR reporter in human cells
Testing Shuttle Peptides ability to deliver GFP-NLS to airway epithelia. - Experiment [Delivery Systems Initiative] - [In Vivo] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides McCray Paul B Delivery of GFP via shuttle peptides to mouse airway epithelium via nasal instilation. Delivery efficiency was quantified in large and small airways by counting the number of GFP positive cells divided by the number of DAPI cells. Projects Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells. - Experiment [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides McCray Paul B In Vitro shuttle peptide delivery of Cas12a RNPs targeting human CFTR and HPRT genes in human primary airway epithelia. Editing efficiency was assessed after 72hrs by sanger sequencing. Projects Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
SpCas9 (Feldan Therapeutics) - Genome Editor [Delivery Systems Initiative] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells.
CM18-PTD4 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide Experiments Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells. Gene editing in vitro by various peptide variants delivering Cas12a in NK cells.
FS48 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells
FSD112 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD115d1 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD121 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD122 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD168 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD168d10 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD168d11 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD228 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD236 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD239 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD259 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD303 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD315 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD323 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD331 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD335 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD339 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD341 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD359 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD362 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD57 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD95 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSX7 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
g-loxPbot_C12a - Guide [Delivery Systems Initiative] - [In Vivo] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides This sgRNA targets the Ai9 and related transgenes at two sites Experiments Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia
Testing newly chemically modified crRNA and tracrRNA to activate the TLR reporter in human cells - Experiment [Delivery Systems Initiative] - [In Vitro] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Sontheimer Erik J Chemically modified crRNA and tracrRNA were delivered by electroporation in presence of amphiphilic peptide to transgenic human HEK-293T cells harboring the TLR-MCV1 reporter. Gene editing was determined by reporter activation. Projects Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
TLR-MCV1 - Model System [Delivery Systems Initiative] - [In Vivo] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors TLR-MCV1 transgene knocked into Rosa26 locus Experiments Delivery of RNP containing chemically modified crRNA C20 with chemically modified tracrRNA T2-PS to determine the RNP distribution in TLR-MCV mouse brain
AsCas12a (IDT and Feldan Therapeutics) - Genome Editor [Delivery Systems Initiative] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Experiments Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia
FS66d6 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD114d1 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD115 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD147 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD188 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD189 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD190 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD193 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD196 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD197 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD222 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD234 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD240 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD260 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD284 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD288 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD293 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD308 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD316 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD321 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD360 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD365 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD57d4 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD97 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
S10D - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
S18 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide Experiments Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells. Gene editing in vitro by various peptide variants delivering Cas12a in NK cells.
g-45_C12a - Guide [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides gRNA targeting CFTR inton-22-23 Experiments Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells.
Delivery of RNP containing chemically modified crRNA C20 with chemically modified tracrRNA T2-PS to determine the RNP distribution in TLR-MCV mouse brain - Experiment [Delivery Systems Initiative] - [In Vivo] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Sontheimer Erik J Chemically modified crRNA and tracrRNA were injected into the striatum of TLR-MCV mice and the distribution of RNP was imaged. Projects Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
STS119 (TLR-MCV1b; Sp_t2:Sp_c20_TLR_MCV1b) - Guide [Delivery Systems Initiative] - [In Vitro] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Targets traffic light reporter transgene Experiments Testing newly chemically modified crRNA and tracrRNA to activate the TLR reporter in human cells
STS134 (PCSK9b; Sp_t2:Sp_c20_PCSK9b) - Guide [Delivery Systems Initiative] - [In Vitro] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Targets endogenous mouse locus Experiments Testing newly chemically modified crRNA and tracrRNA in mouse Hepa 1-6 cells
STS135 (PCSK9c; Sp_t2:Sp_c20_PCSK9c) - Guide [Delivery Systems Initiative] - [In Vitro] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Targets endogenous mouse locus Experiments Testing newly chemically modified crRNA and tracrRNA in mouse Hepa 1-6 cells
Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia - Experiment [Delivery Systems Initiative] - [In Vivo] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides McCray Paul B In vivo shuttle peptide delivery of Cas9 and Cas12a RNPs in mouse airway epithelia. Gene editing was quantified by the GFP+ cells in large and small airways following 1 delivery of GFP protein by GFP positive cells compared to DAPI stained cells. Projects Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
GFP-NLS - Genome Editor [Delivery Systems Initiative] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Nuclear targeted GFP Experiments Testing Shuttle Peptides ability to deliver GFP-NLS to airway epithelia.
FSD114 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD116d1 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD118 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD132 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD168d12 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD195 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD199 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD215 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD237 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD262 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD285 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD286 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD297 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD310 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD319 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD322 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD332 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD364 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD366 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD63 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD67 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD94 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD96 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSX5 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
S10-MOD - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
S85 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide Experiments Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells. Gene editing in vitro by various peptide variants delivering Cas12a in NK cells.
mTmG mouse - Model System [Delivery Systems Initiative] - [In Vivo] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides ROSAmT/mG is a cell membrane-targeted, two-color fluorescent Cre-reporter allele. Prior to Cre recombination, cell membrane-localized tdTomato (mT) fluorescence expression is widespread in cells/tissues. Cre recombinase expressing cells (and future cell lineages derived from these cells) have cell membrane-localized EGFP (mG) fluorescence expression replacing the red fluorescence Experiments Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia
Testing newly discovered Biggie Phage editors in human cells - Experiment [Genome Editors] - [In Vitro] Matched on: --> Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems Banfield Jillian , Doudna Jennifer A Projects Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Cas12j-GFP23 (CasΦ-1) - Guide [Genome Editors] - [In Vitro] Matched on: --> Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems Guide targeting eGFP compatible with CasΦ-1 Experiments Testing newly discovered Biggie Phage editors in human cells
Cas12j-GFP3 (CasΦ-2) - Guide [Genome Editors] - [In Vitro] Matched on: --> Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems Guide targeting eGFP compatible with CasΦ-2 Experiments Testing newly discovered Biggie Phage editors in human cells
Cas12j-GFP8 (CasΦ-3) - Guide [Genome Editors] - [In Vitro] Matched on: --> Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems Guide targeting eGFP compatible with CasΦ-3 Experiments Testing newly discovered Biggie Phage editors in human cells
Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells - Experiment [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides McCray Paul B Delivery of Cas12a RNP targeting human CFTR in Primary human epithelia cells. Gene editing efficiency was determined by percentage of NGS reads that showed an indel Projects Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Alt-R® S.p. Cas9 Nuclease V3 - Genome Editor [Delivery Systems Initiative] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Experiments Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia
FSD168 Scr. - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD186 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD191 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD216 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD227 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD283 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD287 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD289 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD301 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD304 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD306 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD317 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD329 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD333 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD334 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD347 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD368 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD57d1 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD57d5 - Delivery System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia Experiments Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
3849 45-5' - Guide [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Targets endogenous human locus Experiments Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
g-38330_C12a - Guide [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides gRNA targeting HPRT locus Experiments Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells.
NKG2A - Guide [Delivery Systems Initiative] - [In Vitro] Matched on: --> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides gRNA targeting human NKG2A exon 3 Experiments Gene editing in vitro by various peptide variants delivering Cas12a in NK cells.
STS120 (TLR1; Sp_t2:Sp_c20_TLR1) - Guide [Delivery Systems Initiative] - [In Vitro] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Targets traffic light reporter transgene Experiments Testing newly chemically modified crRNA and tracrRNA to activate the TLR1 reporter in human cells
Neuro 2A - Model System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Stable cell line Experiments Testing newly chemically modified crRNA and tracrRNA in mouse Neuro 2A cells
Testing newly chemically modified crRNA and tracrRNA in mouse Hepa 1-6 cells - Experiment [Delivery Systems Initiative] - [In Vitro] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Sontheimer Erik J Chemically modified crRNA and tracrRNA were delivered by electroporation to mouse Hepa 1-6 cells. Editing activity was determined by Sanger sequencing Projects Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
STS118 (TLR-MCV1a; Sp_t2:Sp_c20_TLR_MCV1a) - Guide [Delivery Systems Initiative] - [In Vitro] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Targets traffic light reporter transgene Experiments Testing newly chemically modified crRNA and tracrRNA to activate the TLR reporter in human cells
HEK-293T-disrupted_GFP with MCV-mcherry-Puro - Model System [Delivery Systems Initiative] - [In Vitro] Matched on: --> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors HEK293T cells with an integrated reporter for TLR-MCV1 reporter editing Experiments Testing newly chemically modified crRNA and tracrRNA to activate the TLR reporter in human cells

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283 results for crispr

283 results for crispr

Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. - Experiment [Delivery Systems Initiative] - [In Vivo]

Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors - Project [Delivery Systems Initiative]

Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides - Project [Delivery Systems Initiative]

Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain - Project [Delivery Systems Initiative]

[Validation] Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain - Experiment [Small Animal Testing Center (SATC)] - [In Vivo]

Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems - Project [Genome Editors]

[Validation] Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides - Experiment [Small Animal Testing Center (SATC)] - [In Vivo]

[Validation] Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear - Experiment [Small Animal Testing Center (SATC)] - [In Vivo]

[Validation] Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear - Experiment [Small Animal Testing Center (SATC)] - [In Vivo]

Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) - Experiment [Delivery Systems Initiative] - [In Vivo]

Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter) - Experiment [Delivery Systems Initiative] - [In Vivo]

Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter) - Experiment [Delivery Systems Initiative] - [In Vivo]

Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector. - Experiment [Delivery Systems Initiative] - [In Vivo]

STS159 (mTmG; Sp_t2:Sp_c20_mTmG) - Guide [Delivery Systems Initiative] - [In Vivo, In Vitro]

D237 - Delivery System [Small Animal Testing Center (SATC)] - [In Vivo]

SpyCas9 g-loxP2_C9 - Guide [Small Animal Testing Center (SATC)] - [In Vivo]

Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain - Experiment [Delivery Systems Initiative] - [In Vivo]

D10 - Delivery System [Small Animal Testing Center (SATC)] - [In Vivo]

STS204 (DNMT1; Sp_t2:Sp_c20_Dnmt1) - Guide [Delivery Systems Initiative] - [In Vivo]

STS159 (mTmG; Sp_t2:Sp_c0_mTmG) - Guide [Delivery Systems Initiative] - [In Vivo]

STS159 (mTmG; Sp_t2-PS:Sp_c0_mTmG) - Guide [Delivery Systems Initiative] - [In Vivo]

STS159 (mTmG; Sp_t2-PS:Sp_c20_mTmG) - Guide [Delivery Systems Initiative] - [In Vivo]

[Validation] Validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing. - Experiment [Small Animal Testing Center (SATC)] - [In Vivo]

AB_2532994 - Antibody [Small Animal Testing Center (SATC)] - [In Vivo]

AB_141637 - Antibody [Small Animal Testing Center (SATC)] - [In Vivo]

RNP-NC-no ligand - Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo]

Ai14 gRNA - Guide [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo]

306-O12B blank - Delivery System [Small Animal Testing Center (SATC)] - [In Vivo]

sNLS-SpCas9-sNLS - Genome Editor [Small Animal Testing Center (SATC), Delivery Systems Initiative]

RNP-NC-CPP - Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo]

Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain - Experiment [Delivery Systems Initiative] - [In Vivo]

STS159 (mTmG; Sp_t2:Sp_c20_mTmG) - Guide [Delivery Systems Initiative] - [In Vivo]

CleanCap® Cas9 mRNA (5moU) - Genome Editor [Small Animal Testing Center (SATC)]

Testing newly chemically modified crRNA and tracrRNA in mTmG mouse embryonic fibroblasts - Experiment [Delivery Systems Initiative] - [In Vitro]

Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain - Experiment [Delivery Systems Initiative] - [In Vivo]

AAVcc47-CMV-SaCas9 - Vector [Delivery Systems Initiative]

Ai14 mouse - Model System [Delivery Systems Initiative] - [In Vivo]

SpyCas9-3xNLS - Genome Editor [Delivery Systems Initiative]

sg298 - Guide [Small Animal Testing Center (SATC)] - [In Vivo]

SaCas9-Lagor - Genome Editor [Delivery Systems Initiative]

RNP-NC-RVG - Delivery System [Small Animal Testing Center (SATC)] - [In Vivo]

AB_141607 - Antibody [Small Animal Testing Center (SATC)] - [In Vivo]

AAV9-Ai9-sgRNA1 + sgRNA2 - Vector [Delivery Systems Initiative]

SpCas9 - Genome Editor [Small Animal Testing Center (SATC)]

AAV9-Ai9-sgRNA2-CB-SaCas9 - Vector [Delivery Systems Initiative]

Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain - Experiment [Delivery Systems Initiative] - [In Vivo]

RRID:AB_2536526 - Antibody [Delivery Systems Initiative] - [In Vivo]

AB_2813835 - Antibody [Small Animal Testing Center (SATC)] - [In Vivo]

AB_2209751 - Antibody [Small Animal Testing Center (SATC)] - [In Vivo]

AB_10711040 - Antibody [Small Animal Testing Center (SATC)] - [In Vivo]

Mouse Embryonic Fibroblasts - Model System [Delivery Systems Initiative] - [In Vitro]

Ai14 mouse (congenic) - Model System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo]

Testing preparation for validation at The Jackson Laboratory Small Animal Testing Center - Experiment [Delivery Systems Initiative] - [In Vivo]

AAV9-CMV-SaCas9 - Vector [Delivery Systems Initiative]

AAVcc47-Ai9-sgRNA1 + sgRNA2 - Vector [Delivery Systems Initiative]

AAVcc47-Ai9-sgRNA1-CB-SaCas9 - Vector [Delivery Systems Initiative]

AAVcc47_pTR_self comp 2xU6-Ai9 guides - Vector [Delivery Systems Initiative]

AAV9-Ai9-sgRNA1-CB-SaCas9 - Vector [Delivery Systems Initiative]

AAVcc47-Ai9-sgRNA2-CB-SaCas9 - Vector [Delivery Systems Initiative]

[Validation] Second site validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain - Experiment [Small Animal Testing Center (SATC)] - [In Vivo]

AAV9_pTR_self comp 2xU6-Ai9 guides - Vector [Delivery Systems Initiative]

Ai9 mouse - Model System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo]

306-O12B - Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo, In Vitro]

RRID:AB_1196615 - Antibody [Delivery Systems Initiative] - [In Vivo]

[Validation] Validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice - Experiment [Small Animal Testing Center (SATC)] - [In Vivo]

S10 - Delivery System [Delivery Systems Initiative] - [In Vivo, In Vitro]

306-S10 - Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo, In Vitro]

SpyCas9-3xNLS - Genome Editor [Delivery Systems Initiative]

Testing Shuttle Peptides ability to deliver GFP-NLS to airway epithelia. - Experiment [Delivery Systems Initiative] - [In Vivo]

Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells. - Experiment [Delivery Systems Initiative] - [In Vitro]

SpCas9 (Feldan Therapeutics) - Genome Editor [Delivery Systems Initiative]

CM18-PTD4 - Delivery System [Delivery Systems Initiative] - [In Vitro]

FS48 - Delivery System [Delivery Systems Initiative] - [In Vitro]

FSD112 - Delivery System [Delivery Systems Initiative] - [In Vitro]

FSD115d1 - Delivery System [Delivery Systems Initiative] - [In Vitro]

FSD121 - Delivery System [Delivery Systems Initiative] - [In Vitro]

FSD122 - Delivery System [Delivery Systems Initiative] - [In Vitro]