282 results for crispr

Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides   - Study
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Name ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B   Date Of Submission: 2020-11-02
View Associated: 
Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.   - Experiment - [In Vivo]
Matched on: study ->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Name ->  Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. description ->  Nanocapusules carrying CRISPR Cas9 RNP with guide RNA targeting the stop sequence in the Ai14 transgene generatedDescription ->  Nanocapusules carrying CRISPR Cas9 RNP with guide RNA targeting the stop sequence in the Ai14 transgene experimentName ->  Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
Gong Shaoqin (Sarah)
Nanocapusules carrying CRISPR Cas9 RNP with guide RNA targeting the stop sequence in the Ai14 transgene are intracerebrally delivered to Ai14 mice and gene editing is measured by gain of tdTomato protein expression.
View Associated: 
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors   - Study
Matched on: study ->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Name ->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J   Date Of Submission: 2021-04-15
View Associated: 
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors   - Study
Matched on: study ->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Name ->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J   Date Of Submission: 2021-10-01
View Associated: 
Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain   - Study
Matched on: study ->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Name ->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain
Gong Shaoqin (Sarah)   Date Of Submission: 2020-10-28
View Associated: 
Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases   - Study
Matched on: study ->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases Name ->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
Gao Guang-Ping   Date Of Submission: 2020-10-20
View Associated: 
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides   - Study
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Name ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B   Date Of Submission: 2020-11-02
View Associated: 
[] Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear   - Experiment - [In Vivo]
Matched on: Name ->  Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear description ->  Delivery of CRISPR/Cas9 via bioreducible lipid nanoparticles (LNPs) to the inner ear in Ai14 mice generatedDescription ->  Delivery of CRISPR/Cas9 via bioreducible lipid nanoparticles (LNPs) to the inner ear in Ai14 mice ..SpCas9 experimentName ->  Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
Murray Stephen A
Delivery of CRISPR/Cas9 via bioreducible lipid nanoparticles (LNPs) to the inner ear in Ai14 mice
View Associated: 
Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter)   - Experiment - [In Vivo]
Matched on: Name ->  Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 experimentName ->  Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
Asokan Aravind
A dual vector strategy was employed: one delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (both single stranded AAV cassettes). This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice. A total dose of 4e12vg was injected into each mouse and vectors mixed in a 1:4 ratio of cas9 to guide RNA (1e12vg of CMV Sacas9 vector and 3e12vg of the sgRNA vector) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
View Associated: 
[] Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear   - Experiment - [In Vivo]
Matched on: Name ->  Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 description ->  Delivery of CRISPR/Cas9 editor via bioreducible lipid nanoparticle to the inner ear in Ai14 mice generatedDescription ->  Delivery of CRISPR/Cas9 editor via bioreducible lipid nanoparticle to the inner ear in Ai14 mice ..SpCas9 experimentName ->  Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9
Murray Stephen A
Delivery of CRISPR/Cas9 editor via bioreducible lipid nanoparticle to the inner ear in Ai14 mice
View Associated: 
[] Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides   - Experiment - [In Vivo]
Matched on: Name ->  Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using description ->  Ribonucleoproteins for CRISPR/Cas9 editing are complexed with amphiphilic peptides for delivery to lung generatedDescription ->  Ribonucleoproteins for CRISPR/Cas9 editing are complexed with amphiphilic peptides for delivery to lung ; transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated ; transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated experimentName ->  Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using
Heaney Jason D
Ribonucleoproteins for CRISPR/Cas9 editing are complexed with amphiphilic peptides for delivery to lung airway epithilia via intranasal instillation into mTmG reporter mice. Editing is detected by production of GFP protein, and green fluorescence in airway linings
View Associated: 
Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems   - Study
Matched on: study ->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems Name ->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Doudna Jennifer A   Date Of Submission: 2020-10-16
View Associated: 
[] Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain   - Experiment - [In Vivo]
Matched on: Name ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to description ->  Delivery of CRISPR/Cas9 via RNP-loaded nanocages to the brain in Ai14 mice generatedDescription ->  Delivery of CRISPR/Cas9 via RNP-loaded nanocages to the brain in Ai14 mice..SpCas9 with N- and C-terminal experimentName ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Murray Stephen A
Delivery of CRISPR/Cas9 via RNP-loaded nanocages to the brain in Ai14 mice
View Associated: 
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter)   - Experiment - [In Vivo]
Matched on: Name ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to
Asokan Aravind
A dual vector strategy was employed: one delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (both single stranded AAV cassettes). This strategy was evaluted with both AAV9 (n=3) and AAVcc47 (n=3) by intravenous injection in Ai9 mice. A total dose of 3e12vg was injected into each mouse (1.5e12vg each vector mixed 1:1) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
View Associated: 
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter)   - Experiment - [In Vivo]
Matched on: Name ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
Asokan Aravind
A dual vector strategy was employed: one delivering a single guide RNA and CB driven SaCas9, and another delivering the second guide RNA and CB driven SaCas9. This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice. A total dose of 2e12vg was injected into each mouse (1e12vg each vector mixed 1:1) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
View Associated: 
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.   - Experiment - [In Vivo]
Matched on: Name ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
Asokan Aravind
A dual vector strategy was employed: one self complementary vector delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (single stranded vector). This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=4) by intravenous injection in Ai9 mice. A total dose of 4e12vg was injected into each mouse and vectors mixed in a 1:1 ratio of cas9 to guide RNA (2e12vg of CMV Sacas9 vector and 2e12vg of the self complementary sgRNA vector) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
View Associated: 
D10   - Delivery System - [In Vivo]
Matched on: generatedDescription ->  transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Name ->  Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using experimentName ->  Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using
View Associated: 
D237   - Delivery System - [In Vivo]
Matched on: generatedDescription ->  transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Name ->  Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using experimentName ->  Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using
View Associated: 
RNP-NC-CPP   - Delivery System - [In Vivo]
Matched on: study ->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Name ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to ; Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. experimentName ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to ; Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a cell penetrating peptide (CPP) from the TAT peptide (GRKKRRQRRRPQ) which lacks cell-type specficity
View Associated: 
STS159 (mTmG; Sp_t2:Sp_c0_mTmG)   - Guide - [In Vivo]
Matched on: study ->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription ->  epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Targets mTmG and related reporter transgenes
View Associated: 
SpyCas9 g-loxP2_C9   - Guide - [In Vivo]
Matched on: generatedDescription ->  transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Name ->  Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using experimentName ->  Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using
This sgRNA targets the mTmG transgene
View Associated: 
Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain   - Experiment - [In Vivo]
Matched on: study ->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription ->  epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated ; epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged.
View Associated: 
[] Validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing.   - Experiment - [In Vivo]
Matched on: description ->  Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery. generatedDescription ->  Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery.
Heaney Jason D
Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery. Detection of editing in non-target tissues.
View Associated: 
Testing newly chemically modified crRNA and tracrRNA in mTmG mouse embryonic fibroblasts   - Experiment - [In Vitro]
Matched on: study ->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription ->  cell line..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated ; cell line..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were delivered by electroporation to embryonic fibroblasts harvested from the mTmG reporter mouse. Gene editing was determined by reporter activation.
View Associated: 
Ai14 gRNA   - Guide - [In Vivo]
Matched on: study ->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Name ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to ; Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. experimentName ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to ; Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
This sgRNA targets the Ai9 and related transgenes at multiple sites
View Associated: 
Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain   - Experiment - [In Vivo]
Matched on: study ->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription ->  transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated ; transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged.
View Associated: 
RNP-NC-no ligand   - Delivery System - [In Vivo]
Matched on: study ->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Name ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to ; Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. experimentName ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to ; Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA.
View Associated: 
Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain   - Experiment - [In Vivo]
Matched on: study ->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription ->  transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated ; transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged.
View Associated: 
AB_1196615    - Antibody - [In Vivo]
Matched on: study ->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription ->  locus......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
GFP (D5.1) XP Rabbit mAb antibody, Cell Signaling Technology
View Associated: 
SpCas9   - Genome Editor
Matched on: generatedDescription ->  NHEJ repair ..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Name ->  Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using experimentName ->  Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using
View Associated: 
sg298   - Guide - [In Vivo]
Matched on: Name ->  Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 ; Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear experimentName ->  Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 ; Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
This sgRNA targets the Ai9 and related transgenes at multiple sites. 2'-O-Methyl at 3 first and last bases, 3' phosphorothioate bonds between first 3 and last 2 bases
View Associated: 
Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain   - Experiment - [In Vivo]
Matched on: study ->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription ->  transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated ; transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged.
View Associated: 
Mouse Embryonic Fibroblasts   - Model System - [In Vitro]
Matched on: study ->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription ->  cell line..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Primary cell line
View Associated: 
sNLS-SpCas9-sNLS   - Genome Editor
Matched on: study ->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Name ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to ; Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. experimentName ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to ; Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
SpCas9 with N- and C-terminal SV40 NLS
View Associated: 
AAVcc47-Ai9-sgRNA1 + sgRNA2   - Vector
Matched on: Name ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus
View Associated: 
[] Second site validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain   - Experiment - [In Vivo]
Matched on: description ->  Validation of delivery of AAV custom designed to cross the blood-brain barrier for CRISPR/Cas9 editing generatedDescription ->  Validation of delivery of AAV custom designed to cross the blood-brain barrier for CRISPR/Cas9 editing
Heaney Jason D
Validation of delivery of AAV custom designed to cross the blood-brain barrier for CRISPR/Cas9 editing. Editing detected and quantified in brain by generation of tdTomato fluorescent protein signal from Ai9 reporter mice
View Associated: 
RNP-NC-RVG   - Delivery System - [In Vivo]
Matched on: Name ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to experimentName ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a RVG peptide YTIWMPENPRPGTPCDIFTNSRGKRASNG which specifically interacts withthe N-acetylecholine receptor (AchR) on neuronal cells, which mediates NP entry
View Associated: 
AB_10711040    - Antibody - [In Vivo]
Matched on: Name ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to experimentName ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Abcam, mouse anti-NeuN
View Associated: 
AB_2813835    - Antibody - [In Vivo]
Matched on: Name ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to experimentName ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Abcam, Donkey anti-rat alexa fluour 647
View Associated: 
Ai14 mouse   - Model System - [In Vivo]
Matched on: study ->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Name ->  Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. experimentName ->  Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain.
View Associated: 
AB_2209751    - Antibody - [In Vivo]
Matched on: study ->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases Name ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to experimentName ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Anti-RFP (RABBIT) Antibody
View Associated: 
CleanCap® Cas9 mRNA (5moU)   - Genome Editor
Matched on: Name ->  Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 ; Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear experimentName ->  Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 ; Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
SpCas9 mRNA with 2 NLS signals, HA tag and capped using CleanCap. It is polyadenylated, substituted with a modified uridine and optimized for mammalian systems. It mimics a fully processed mature mRNA.
View Associated: 
AAV9-CMV-SaCas9   - Vector
Matched on: Name ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
AAV serotype 9 delivering CMV driven SaCas9
View Associated: 
AAV9-Ai9-sgRNA1-CB-SaCas9   - Vector
Matched on: Name ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAV serotype 9 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus
View Associated: 
AAV9-Ai9-sgRNA2-CB-SaCas9   - Vector
Matched on: Name ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAV serotype 9 delivering gRNA 2 + CB SaCas9 targeting the Ai9 locus
View Associated: 
AAVcc47-Ai9-sgRNA1-CB-SaCas9   - Vector
Matched on: Name ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAVcc47 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus
View Associated: 
AAVcc47_pTR_self comp 2xU6-Ai9 guides   - Vector
Matched on: Name ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAVcc47 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 transgene
View Associated: 
Testing preparation for validation at The Jackson Laboratory Small Animal Testing Center   - Experiment - [In Vivo]
Matched on: study ->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription ->  locus......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated ; locus......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Sontheimer Erik J
Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
View Associated: 
SaCas9-Lagor   - Genome Editor
Matched on: Name ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
View Associated: 
AB_2532994    - Antibody - [In Vivo]
Matched on: Name ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to experimentName ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Thermo-Fisher, Rat anti-GFAP
View Associated: 
AB_141637    - Antibody - [In Vivo]
Matched on: Name ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to experimentName ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Invitrogen, Donkey anti-rabbit alexa fluor 594
View Associated: 
AB_141607    - Antibody - [In Vivo]
Matched on: Name ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to experimentName ->  Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Invitrogen, Donkey anti-mouse alexafluor 488
View Associated: 
Ai14 mouse (congenic)   - Model System - [In Vivo]
Matched on: Name ->  Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 ; Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to ; Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear experimentName ->  Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 ; Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to ; Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain.
View Associated: 
Ai9 mouse   - Model System - [In Vivo]
Matched on: study ->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases Name ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus.
View Associated: 
AAV9-Ai9-sgRNA1 + sgRNA2   - Vector
Matched on: Name ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus
View Associated: 
AAVcc47-CMV-SaCas9   - Vector
Matched on: Name ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
AAVcc47 delivering CMV driven SaCas9
View Associated: 
306-O12B blank   - Delivery System - [In Vivo]
Matched on: Name ->  Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 experimentName ->  Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9
Combinatorial library cationic lipid nanoparticles
View Associated: 
306-O12B   - Delivery System - [In Vivo, In Vitro]
Matched on: Name ->  Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 ; Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear experimentName ->  Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 ; Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
Combinatorial library cationic lipid nanoparticles
View Associated: 
AAVcc47-Ai9-sgRNA2-CB-SaCas9   - Vector
Matched on: Name ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAVcc47 delivering sgRNA 2 + CB SaCas9 targeting the Ai9 locus
View Associated: 
SpyCas9-3xNLS   - Genome Editor
Matched on: study ->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription ->  Rosa26 locus ..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
SpyCas9-3xNLS is type II-A Cas9 from Streptococcus pyogenes strain SF370. It was expressed from pMCSG7 bacterial expressing vector and purified from Escherichia coli Rosetta DE3 strain. SpyCas9 fused to 3 NLS: C-Myc-like NLS at the N-terminal SV40 NLS and Nucleoplasmin NLS at the C-terminal
View Associated: 
AAV9_pTR_self comp 2xU6-Ai9 guides   - Vector
Matched on: Name ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to experimentName ->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 transgene
View Associated: 
S10   - Delivery System - [In Vivo, In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides ; Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription ->  epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
306-S10   - Delivery System - [In Vivo, In Vitro]
Matched on: Name ->  Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear experimentName ->  Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
combinatorial library cationic lipid nanoparticles
View Associated: 
SpyCas9-3xNLS   - Genome Editor
Matched on: study ->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription ->  epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated ; cell line..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
SpyCas9-3xNLS is type II-A Cas9 from Streptococcus pyogenes strain SF370. It was expressed from pMCSG7 bacterial expressing vector and purified from Escherichia coli Rosetta DE3 strain. SpyCas9 fused to 3 NLS: C-Myc-like NLS at the N-terminal SV40 NLS and Nucleoplasmin NLS at the C-terminal
View Associated: 
Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells   - Experiment - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B
Delivery of Cas9 RNP targeting human CFTR in Primary human epithelia cells. Gene editing efficiency was determined by percentage of NGS reads that showed an indel
View Associated: 
Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells.   - Experiment - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B
In Vitro shuttle peptide delivery of Cas12a RNPs targeting human CFTR and HPRT genes in human primary airway epithelia. Editing efficiency was assessed after 72hrs by sanger sequencing.
View Associated: 
FSD112   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD114   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD114d1   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD116d1   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD122   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD159   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD186   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD189   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD197   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD227   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD262   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD284   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD287   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD307   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD308   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD310   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD318   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD330   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD366   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD57d5   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD63   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD97   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSX7   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
BALB/c mouse   - Model System - [In Vivo]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
BALB/cJ is a commonly used inbred. Key traits include a susceptibility to developing the demyelinating disease upon infection with Theiler's murine encephalomyelitis virus. The BALB/cJ substrain is susceptible to Listeria, all species of Leishmania, and several species of Trypanosoma, but is resistant to experimental allergic orchitis (EAO).
View Associated: 
HEK-293T with Ai9 transient reporter assay   - Model System - [In Vitro]
Matched on: study ->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
HEK-293T cells transfected with an Ai9 inducible transgene reporter plasmid used to test gene editing activity by fluorescence
View Associated: 
mTmG mouse (congenic)   - Model System - [In Vivo]
Matched on: study ->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription ->  epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Name ->  Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using experimentName ->  Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using
mTmG is a double-fluorescent reporter transgenic mouse which expresses membrane-targeted tdTomato flanked by loxP sequences, followed by membrane-targeted GFP. After genomic cleavage by Cas9 at two sites, or Cre recombinase between loxP sites, tdTomato expression is lost and GFP is expressed.
View Associated: 
FSD117D1   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides ; Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells   - Experiment - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B
Delivery of Cas12a RNP targeting human CFTR in Primary human epithelia cells. Gene editing efficiency was determined by percentage of NGS reads that showed an indel
View Associated: 
Gene editing in vitro by various peptide variants delivering Cas12a in NK cells.   - Experiment - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B
In Vitro shuttle peptide delivery of Cas12a RNP targeting human NKG2A gene in human NK cells. Gene editing was assessed after 48hr by T7E1 digestion.
View Associated: 
Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia   - Experiment - [In Vivo]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B
In vivo shuttle peptide delivery of Cas9 and Cas12a RNPs in mouse airway epithelia. Gene editing was quantified by the GFP+ cells in large and small airways following 1 delivery of GFP protein by GFP positive cells compared to DAPI stained cells.
View Associated: 
AsCas12a (IDT and Feldan Therapeutics)   - Genome Editor
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
View Associated: 
Cre recombinase   - Genome Editor
Matched on: study ->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
Cre recombinase delivered by scAAV5-Cre-GFP plasmid
View Associated: 
FSD115   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD121   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD168 cyclic   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD168d10   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD168d11   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD193   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD228   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD236   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD259   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD283   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD288   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD297   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD303   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD319   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD321   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD359   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD360   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD365   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD367   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD57d1   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSX2   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSX4   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
S10D   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
S10-MOD   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
AAV.pU1a-SpCas9   - Vector
Matched on: study ->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
Expresses codon-optimized SpCas9 in mammalian cells. HA-SV40NLS-SpCas9-SV40NLS
View Associated: 
H509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1)   - Vector
Matched on: study ->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
AAV2/5 expressing SpyCas9. AAV2/5 expressing two sgRNAs under U6 promoter and eGFP
View Associated: 
g-loxP2_C9   - Guide - [In Vivo]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
This sgRNA targets the Ai9 and related transgenes
View Associated: 
NKG2A   - Guide - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
gRNA targeting human NKG2A exon 3
View Associated: 
Testing newly chemically modified crRNA and tracrRNA to activate the TLR reporter in human cells   - Experiment - [In Vitro]
Matched on: study ->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were delivered by electroporation in presence of amphiphilic peptide to transgenic human HEK-293T cells harboring the TLR-MCV1 reporter. Gene editing was determined by reporter activation.
View Associated: 
Testing newly chemically modified crRNA and tracrRNA in mouse Neuro 2A cells   - Experiment - [In Vitro]
Matched on: study ->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were delivered by electroporation to mouse Neuro2A cells. Editing activity was determined by Sanger sequencing
View Associated: 
STS118 (TLR-MCV1a; Sp_t2:Sp_c20_TLR_MCV1a)   - Guide - [In Vitro]
Matched on: study ->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets traffic light reporter transgene
View Associated: 
CM18-PTD4   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide
View Associated: 
FS66d6   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD132   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD168   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD168d12   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD168d20   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide
View Associated: 
FSD168 Disul.   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD168 Scr.   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD190   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD199   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD238   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD286   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD289   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD291   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD301   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD315   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD332   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD335   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD363   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSD364   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSX3   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
FSX8   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
View Associated: 
S18   - Delivery System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide
View Associated: 
pH509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1)   - Vector
Matched on: study ->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
AAV2/5 expressing SpyCas9. AAV2/5 expressing two sgRNAs under U6 promoter and eGFP
View Associated: 
McCray_CFTR_Cas9_Guide1   - Guide - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Targets endogenous human locus
View Associated: 
sgAi9L   - Guide - [In Vitro]
Matched on: study ->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
This sgRNA targets the Ai9 and related transgenes
View Associated: 
sgAi9R   - Guide - [In Vitro]
Matched on: study ->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
This sgRNA targets the Ai9 and related transgenes
View Associated: 
NK   - Model System - [In Vitro]
Matched on: study ->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
View Associated: 
Testing newly chemically modified crRNA and tracrRNA in mouse Hepa 1-6 cells   - Experiment - [In Vitro]
Matched on: study ->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were delivered by electroporation to mouse Hepa 1-6 cells. Editing activity was determined by Sanger sequencing
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HEK-293T-disrupted_GFP-mcherry-Puro   - Model System - [In Vitro]
Matched on: study ->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
HEK293T cells with an integrated reporter for TLR1 reporter editing
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