-->
Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain
-->
Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
-->
Nanocapusules carrying CRISPR Cas9 RNP with guide RNA targeting the stop sequence in the Ai14 transgene
-->
Nanocapusules carrying CRISPR Cas9 RNP with guide RNA targeting the stop sequence in the Ai14 transgene
name -->
Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
-->
Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
Gong Shaoqin (Sarah)
Nanocapusules carrying CRISPR Cas9 RNP with guide RNA targeting the stop sequence in the Ai14 transgene are intracerebrally delivered to Ai14 mice and gene editing is measured by gain of tdTomato protein expression.
|
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
-->
RNA-guided CRISPR genome editing systems promise to revolutionize the treatment of inherited disease.
that directs editing is a critical hurdle in the development of clinical applications for engineered CRISPR
of nucleic acid therapeutics, we have established a framework for complete chemical modification of CRISPR
name -->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J
RNA-guided CRISPR genome editing systems promise to revolutionize the treatment of inherited disease. Safe, effective, and target-tissue-specific delivery of the guide RNA that directs editing is a critical hurdle in the development of clinical applications for engineered CRISPR systems. Using strategies validated for the delivery of other categories of nucleic acid therapeutics, we have established a framework for complete chemical modification of CRISPR guides, thereby conferring in vivo stability and effective biodistribution properties. The proposed research will optimize these guides, as well as other editing components, for clinical use.
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-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
name -->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B
The proposed research is relevant to the public health because genetic and acquired diseases affecting the airways pose major disease and economic burdens. By advancing the delivery of gene editing tools, it may be possible to therapeutically modify the cells lining the airways. This novel strategy has implications for the treatment of both monogenetic and acquired lungs disease, and may have applications for other somatic cell therapies.
|
-->
Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain
-->
In vivo genome editing using CRISPR/Cas9 is anticipated to be the next wave of therapeutics for various
name -->
Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain
Gong Shaoqin (Sarah)
In vivo genome editing using CRISPR/Cas9 is anticipated to be the next wave of therapeutics for various major health threats, including neurodegenerative diseases. However, to date, very few Cas9-gRNA ribonucleoprotein in vivo delivery methods have been reported, and delivery to the brain has been particularly challenging. The unique nanocapsules we plan to develop will ultimately enable high efficiency neuron-targeted genome editing in the brain, thereby offering new hope to treat devastating neurodegenerative diseases.
|
-->
Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
-->
Delivery of CRISPR/Cas9 via RNP-loaded nanocages to the brain in Ai14 mice
-->
Delivery of CRISPR/Cas9 via RNP-loaded nanocages to the brain in Ai14 mice..SpCas9 with N- and C-terminal
name -->
Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
-->
Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Murray Stephen A
Delivery of CRISPR/Cas9 via RNP-loaded nanocages to the brain in Ai14 mice
|
-->
Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
-->
Expanding genome editing tools through exploration of new CRISPR-Cas proteins and DNA repair enzymes
NARRATIVE Fundamental research on bacterial adaptive immunity uncovered the genome editing properties of CRISPR-Cas
We will focus our investigation on newly described CRISPR-Cas systems and DNA-interacting proteins that
name -->
Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Banfield Jillian
, Doudna Jennifer A
Expanding genome editing tools through exploration of new CRISPR-Cas proteins and DNA repair enzymes NARRATIVE Fundamental research on bacterial adaptive immunity uncovered the genome editing properties of CRISPR-Cas systems, and it is clear that uncultivated microbes contain more pathways and enzymes that may be useful as genome editing tools. We will combine bioinformatics and biochemistry to identify new DNA- and RNA-associating proteins and will analyze their mechanisms of action. We will focus our investigation on newly described CRISPR-Cas systems and DNA-interacting proteins that occur in conserved genomic context.
|
-->
Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using
-->
Ribonucleoproteins for CRISPR/Cas9 editing are complexed with amphiphilic peptides for delivery to lung
-->
Ribonucleoproteins for CRISPR/Cas9 editing are complexed with amphiphilic peptides for delivery to lung
transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
name -->
Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using
-->
Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using
Heaney Jason D
Ribonucleoproteins for CRISPR/Cas9 editing are complexed with amphiphilic peptides for delivery to lung airway epithilia via intranasal instillation into mTmG reporter mice. Editing is detected by production of GFP protein, and green fluorescence in airway linings
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-->
Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
-->
Delivery of CRISPR/Cas9 via bioreducible lipid nanoparticles (LNPs) to the inner ear in Ai14 mice
-->
Delivery of CRISPR/Cas9 via bioreducible lipid nanoparticles (LNPs) to the inner ear in Ai14 mice ..SpCas9
name -->
Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
-->
Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
Murray Stephen A
Delivery of CRISPR/Cas9 via bioreducible lipid nanoparticles (LNPs) to the inner ear in Ai14 mice
|
-->
Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9
-->
Delivery of CRISPR/Cas9 editor via bioreducible lipid nanoparticle to the inner ear in Ai14 mice
-->
Delivery of CRISPR/Cas9 editor via bioreducible lipid nanoparticle to the inner ear in Ai14 mice ..SpCas9
name -->
Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9
-->
Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9
Murray Stephen A
Delivery of CRISPR/Cas9 editor via bioreducible lipid nanoparticle to the inner ear in Ai14 mice
|
-->
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to
name -->
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to
-->
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to
Asokan Aravind
A dual vector strategy was employed: one delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (both single stranded AAV cassettes). This strategy was evaluted with both AAV9 (n=3) and AAVcc47 (n=3) by intravenous injection in Ai9 mice. A total dose of 3e12vg was injected into each mouse (1.5e12vg each vector mixed 1:1) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
|
-->
Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
name -->
Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
-->
Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
Asokan Aravind
A dual vector strategy was employed: one delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (both single stranded AAV cassettes). This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice. A total dose of 4e12vg was injected into each mouse and vectors mixed in a 1:4 ratio of cas9 to guide RNA (1e12vg of CMV Sacas9 vector and 3e12vg of the sgRNA vector) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
|
-->
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
name -->
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
-->
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
Asokan Aravind
A dual vector strategy was employed: one delivering a single guide RNA and CB driven SaCas9, and another delivering the second guide RNA and CB driven SaCas9. This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice. A total dose of 2e12vg was injected into each mouse (1e12vg each vector mixed 1:1) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
|
-->
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
name -->
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
-->
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
Asokan Aravind
A dual vector strategy was employed: one self complementary vector delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (single stranded vector). This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=4) by intravenous injection in Ai9 mice. A total dose of 4e12vg was injected into each mouse and vectors mixed in a 1:1 ratio of cas9 to guide RNA (2e12vg of CMV Sacas9 vector and 2e12vg of the self complementary sgRNA vector) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.
|
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
-->
transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
cell line..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Targets mTmG and related reporter transgenes
|
D237
- Delivery System [Small Animal Testing Center (SATC)]
- [In Vivo]
-->
transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
-->
Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using
|
SpyCas9 g-loxP2_C9
- Guide [Small Animal Testing Center (SATC)]
- [In Vivo]
-->
transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
-->
Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using
This sgRNA targets the mTmG transgene
|
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
-->
transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged.
|
D10
- Delivery System [Small Animal Testing Center (SATC)]
- [In Vivo]
-->
transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
-->
Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using
|
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
-->
locus......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
For endogenous locus
|
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
-->
epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Targets mTmG and related reporter transgenes
|
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
-->
transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Targets mTmG and related reporter transgenes
|
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
-->
transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Targets mTmG and related reporter transgenes
|
-->
Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery.
-->
Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery.
Heaney Jason D
Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery. Detection of editing in non-target tissues.
|
AB_2532994
- Antibody [Small Animal Testing Center (SATC)]
- [In Vivo]
-->
Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Other Id:
13-0300Â
Thermo-Fisher, Rat anti-GFAP
|
AB_141637
- Antibody [Small Animal Testing Center (SATC)]
- [In Vivo]
-->
Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Other Id:
A21207
Invitrogen, Donkey anti-rabbit alexa fluor 594
|
RNP-NC-no ligand
- Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative]
- [In Vivo]
-->
Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain
-->
Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA.
|
Ai14 gRNA
- Guide [Small Animal Testing Center (SATC), Delivery Systems Initiative]
- [In Vivo]
-->
Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain
-->
Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
This sgRNA targets the Ai9 and related transgenes at multiple sites
|
306-O12B blank
- Delivery System [Small Animal Testing Center (SATC)]
- [In Vivo]
-->
Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9
Combinatorial library cationic lipid nanoparticles
|
sNLS-SpCas9-sNLS
- Genome Editor
[Small Animal Testing Center (SATC), Delivery Systems Initiative]
-->
Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain
-->
Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
SpCas9 with N- and C-terminal SV40 NLS
|
RNP-NC-CPP
- Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative]
- [In Vivo]
-->
Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain
-->
Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a cell penetrating peptide (CPP) from the TAT peptide (GRKKRRQRRRPQ) which lacks cell-type specficity
|
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
-->
transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged.
|
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
-->
transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Targets mTmG and related reporter transgenes
|
-->
Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9
Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
SpCas9 mRNA with 2 NLS signals, HA tag and capped using CleanCap. It is polyadenylated, substituted with a modified uridine and optimized for mammalian systems. It mimics a fully processed mature mRNA.
|
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
-->
cell line..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
cell line..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were delivered by electroporation to embryonic fibroblasts harvested from the mTmG reporter mouse. Gene editing was determined by reporter activation.
|
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
-->
epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged.
|
AAVcc47-CMV-SaCas9
- Vector
[Delivery Systems Initiative]
-->
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to
Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
AAVcc47 delivering CMV driven SaCas9
|
Ai14 mouse
- Model System [Delivery Systems Initiative]
- [In Vivo]
-->
Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain
-->
Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain.
|
SpyCas9-3xNLS
- Genome Editor
[Delivery Systems Initiative]
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
-->
Rosa26 locus ..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
SpyCas9-3xNLS is type II-A Cas9 from Streptococcus pyogenes strain SF370. It was expressed from
pMCSG7 bacterial expressing vector and purified from Escherichia coli Rosetta DE3 strain.
SpyCas9 fused to 3 NLS:
C-Myc-like NLS at the N-terminal
SV40 NLS and Nucleoplasmin NLS at the C-terminal
|
sg298
- Guide [Small Animal Testing Center (SATC)]
- [In Vivo]
-->
Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9
Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
This sgRNA targets the Ai9 and related transgenes at multiple sites. 2'-O-Methyl at 3 first and last bases, 3' phosphorothioate bonds between first 3 and last 2 bases
|
SaCas9-Lagor
- Genome Editor
[Delivery Systems Initiative]
-->
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
|
RNP-NC-RVG
- Delivery System [Small Animal Testing Center (SATC)]
- [In Vivo]
-->
Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a RVG peptide YTIWMPENPRPGTPCDIFTNSRGKRASNG which specifically interacts withthe N-acetylecholine receptor (AchR) on neuronal cells, which mediates NP entry
|
AB_141607
- Antibody [Small Animal Testing Center (SATC)]
- [In Vivo]
-->
Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Other Id:
A21202
Invitrogen, Donkey anti-mouse alexafluor 488
|
-->
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to
Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus
|
SpCas9
- Genome Editor
[Small Animal Testing Center (SATC)]
-->
recombination...mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
-->
Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using
|
-->
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAV serotype 9 delivering gRNA 2 + CB SaCas9 targeting the Ai9 locus
|
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
-->
transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged.
|
RRID:AB_2536526
- Antibody [Delivery Systems Initiative]
- [In Vivo]
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
-->
transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Other Id:
AB_2536526
GFP Recombinant Rabbit Monoclonal Antibody, Thermo Fisher Scientific #G10362
|
AB_2813835
- Antibody [Small Animal Testing Center (SATC)]
- [In Vivo]
-->
Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Other Id:
ab150155
Abcam, Donkey anti-rat alexa fluour 647
|
AB_2209751
- Antibody [Small Animal Testing Center (SATC)]
- [In Vivo]
-->
Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Other Id:
Rockland Cat# 600-401-379
Anti-RFP (RABBIT) Antibody
|
AB_10711040
- Antibody [Small Animal Testing Center (SATC)]
- [In Vivo]
-->
Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Other Id:
ab104224Â
Abcam, mouse anti-NeuN
|
Mouse Embryonic Fibroblasts
- Model System [Delivery Systems Initiative]
- [In Vitro]
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
-->
cell line..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Primary cell line
|
Ai14 mouse (congenic)
- Model System [Small Animal Testing Center (SATC), Delivery Systems Initiative]
- [In Vivo]
-->
Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9
Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain.
|
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
-->
locus......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
locus......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Sontheimer Erik J
Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
|
AAV9-CMV-SaCas9
- Vector
[Delivery Systems Initiative]
-->
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
AAV serotype 9 delivering CMV driven SaCas9
|
-->
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to
Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus
|
-->
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAVcc47 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus
|
-->
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAVcc47 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 transgene
|
-->
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAV serotype 9 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus
|
-->
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAVcc47 delivering sgRNA 2 + CB SaCas9 targeting the Ai9 locus
|
-->
Validation of delivery of AAV custom designed to cross the blood-brain barrier for CRISPR/Cas9 editing
-->
Validation of delivery of AAV custom designed to cross the blood-brain barrier for CRISPR/Cas9 editing
Heaney Jason D
Validation of delivery of AAV custom designed to cross the blood-brain barrier for CRISPR/Cas9 editing. Editing detected and quantified in brain by generation of tdTomato fluorescent protein signal from Ai9 reporter mice
|
-->
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 transgene
|
Ai9 mouse
- Model System [Small Animal Testing Center (SATC), Delivery Systems Initiative]
- [In Vivo]
-->
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus.
|
306-O12B
- Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative]
- [In Vivo, In Vitro]
-->
Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9
Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
Combinatorial library cationic lipid nanoparticles
|
RRID:AB_1196615
- Antibody [Delivery Systems Initiative]
- [In Vivo]
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
-->
epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Other Id:
AB_1196615
GFP (D5.1) XP Rabbit mAb antibody, Cell Signaling Technology
|
-->
AAV5 encoding CRISPR/Cas editing machinery were delivered to the lungs of reporter mice by intratracheal
-->
AAV5 encoding CRISPR/Cas editing machinery were delivered to the lungs of reporter mice by intratracheal
Heaney Jason D
AAV5 encoding CRISPR/Cas editing machinery were delivered to the lungs of reporter mice by intratracheal instillation. After 4 weeks incubation, the mice were dissected and the lungs imaged for the presence of tdTomato fluorescence, indicating successful editing. Editing calculated by dividing the number of tdTomato+ red cells by the number of nuclei in each airway
|
S10
- Delivery System [Delivery Systems Initiative]
- [In Vivo, In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
-->
epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
Shuttle peptide used to deliver reagents to airway epithelia
|
306-S10
- Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative]
- [In Vivo, In Vitro]
-->
Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
combinatorial library cationic lipid nanoparticles
|
SpyCas9-3xNLS
- Genome Editor
[Delivery Systems Initiative]
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
-->
epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
cell line..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated
SpyCas9-3xNLS is type II-A Cas9 from Streptococcus pyogenes strain SF370. It was expressed from
pMCSG7 bacterial expressing vector and purified from Escherichia coli Rosetta DE3 strain.
SpyCas9 fused to 3 NLS:
C-Myc-like NLS at the N-terminal
SV40 NLS and Nucleoplasmin NLS at the C-terminal
|
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B
Delivery of GFP via shuttle peptides to mouse airway epithelium via nasal instilation. Delivery efficiency was quantified in large and small airways by counting the number of GFP positive cells divided by the number of DAPI cells.
|
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B
In Vitro shuttle peptide delivery of Cas12a RNPs targeting human CFTR and HPRT genes in human primary airway epithelia. Editing efficiency was assessed after 72hrs by sanger sequencing.
|
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
|
CM18-PTD4
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide
|
FS48
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD112
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD115d1
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD121
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD122
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD168
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD168d10
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD168d11
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD228
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD236
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD239
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD259
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD303
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD315
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD323
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD331
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD335
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD339
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD341
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD359
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD362
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD57
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD95
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSX7
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
g-loxPbot_C12a
- Guide [Delivery Systems Initiative]
- [In Vivo]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
This sgRNA targets the Ai9 and related transgenes at two sites
|
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were delivered by electroporation in presence of amphiphilic peptide to transgenic human HEK-293T cells harboring the TLR-MCV1 reporter. Gene editing was determined by reporter activation.
|
TLR-MCV1
- Model System [Delivery Systems Initiative]
- [In Vivo]
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
TLR-MCV1 transgene knocked into Rosa26 locus
|
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
|
FS66d6
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD114d1
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD115
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD147
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD188
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD189
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD190
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD193
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD196
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD197
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD222
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD234
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD240
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD260
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD284
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD288
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD293
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD308
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD316
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD321
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD360
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD365
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD57d4
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD97
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
S10D
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
S18
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide
|
g-45_C12a
- Guide [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
gRNA targeting CFTR inton-22-23
|
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were injected into the striatum of TLR-MCV mice and the distribution of RNP was imaged.
|
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets traffic light reporter transgene
|
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets endogenous mouse locus
|
-->
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets endogenous mouse locus
|
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B
In vivo shuttle peptide delivery of Cas9 and Cas12a RNPs in mouse airway epithelia. Gene editing was quantified by the GFP+ cells in large and small airways following 1 delivery of GFP protein by GFP positive cells compared to DAPI stained cells.
|
GFP-NLS
- Genome Editor
[Delivery Systems Initiative]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Nuclear targeted GFP
|
FSD114
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD116d1
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD118
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD132
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD168d12
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD195
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD199
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD215
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD237
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD262
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD285
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD286
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD297
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD310
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD319
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD322
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD332
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD364
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD366
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD63
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD67
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD94
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD96
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSX5
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
S10-MOD
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
S85
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide
|
mTmG mouse
- Model System [Delivery Systems Initiative]
- [In Vivo]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
ROSAmT/mGÂ is a cell membrane-targeted, two-color fluorescent Cre-reporter allele. Prior to Cre recombination, cell membrane-localized tdTomato (mT) fluorescence expression is widespread in cells/tissues. Cre recombinase expressing cells (and future cell lineages derived from these cells) have cell membrane-localized EGFP (mG) fluorescence expression replacing the red fluorescence
|
-->
Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Banfield Jillian
, Doudna Jennifer A
|
-->
Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasΦ-1
|
-->
Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasΦ-2
|
-->
Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasΦ-3
|
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B
Delivery of Cas12a RNP targeting human CFTR in Primary human epithelia cells. Gene editing efficiency was determined by percentage of NGS reads that showed an indel
|
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
|
FSD168 Scr.
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD186
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD191
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD216
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD227
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD283
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD287
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD289
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD301
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD304
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD306
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD317
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
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FSD329
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
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Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD333
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD334
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD347
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD368
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD57d1
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
FSD57d5
- Delivery System [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia
|
3849 45-5'
- Guide [Delivery Systems Initiative]
- [In Vitro]
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Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Targets endogenous human locus
|
g-38330_C12a
- Guide [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
gRNA targeting HPRT locus
|
NKG2A
- Guide [Delivery Systems Initiative]
- [In Vitro]
-->
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
gRNA targeting human NKG2A exon 3
|
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Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets traffic light reporter transgene
|
Neuro 2A
- Model System [Delivery Systems Initiative]
- [In Vitro]
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Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Stable cell line
|
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Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J
Chemically modified crRNA and tracrRNA were delivered by electroporation to mouse Hepa 1-6 cells. Editing activity was determined by Sanger sequencing
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Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets traffic light reporter transgene
|
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Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
HEK293T cells with an integrated reporter for TLR-MCV1 reporter editing
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