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Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides - Study Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Name -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides McCray Paul B Date Of Submission: 2020-11-02 View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. - Experiment - [In Vivo] Matched on: study -> Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Name -> Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. description -> Nanocapusules carrying CRISPR Cas9 RNP with guide RNA targeting the stop sequence in the Ai14 transgene generatedDescription -> Nanocapusules carrying CRISPR Cas9 RNP with guide RNA targeting the stop sequence in the Ai14 transgene experimentName -> Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. Gong Shaoqin (Sarah) Nanocapusules carrying CRISPR Cas9 RNP with guide RNA targeting the stop sequence in the Ai14 transgene are intracerebrally delivered to Ai14 mice and gene editing is measured by gain of tdTomato protein expression. View Associated:
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors - Study Matched on: study -> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Name -> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Sontheimer Erik J Date Of Submission: 2021-04-15 View Associated: Testing newly chemically modified crRNA and tracrRNA in mTmG mouse embryonic fibroblasts Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain Testing newly chemically modified crRNA and tracrRNA to activate the TLR1 reporter in human cells Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain Testing newly chemically modified crRNA and tracrRNA in mouse Hepa 1-6 cells Delivery of RNP containing chemically modified crRNA C20 with chemically modified tracrRNA T2-PS to determine the RNP distribution in TLR-MCV mouse brain Testing newly chemically modified crRNA and tracrRNA in mouse Neuro 2A cells Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain Testing newly chemically modified crRNA and tracrRNA to activate the TLR reporter in human cells Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors - Study Matched on: study -> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Name -> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Sontheimer Erik J Date Of Submission: 2021-10-01 View Associated:
Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain - Study Matched on: study -> Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Name -> Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Gong Shaoqin (Sarah) Date Of Submission: 2020-10-28 View Associated:
Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases - Study Matched on: study -> Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases Name -> Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases Gao Guang-Ping Date Of Submission: 2020-10-20 View Associated: Testing AAV5 for activation of tdTomato in HEK293T cells Testing AAV5 for activation of tdTomato in mouse airway
Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides - Study Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Name -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides McCray Paul B Date Of Submission: 2020-11-02 View Associated: Testing Shuttle Peptides ability to deliver GFP-NLS to airway epithelia. Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells. Gene editing in vitro by various peptide variants delivering Cas12a in NK cells.
[] Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear - Experiment - [In Vivo] Matched on: Name -> Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear description -> Delivery of CRISPR/Cas9 via bioreducible lipid nanoparticles (LNPs) to the inner ear in Ai14 mice generatedDescription -> Delivery of CRISPR/Cas9 via bioreducible lipid nanoparticles (LNPs) to the inner ear in Ai14 mice ..SpCas9 experimentName -> Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Murray Stephen A Delivery of CRISPR/Cas9 via bioreducible lipid nanoparticles (LNPs) to the inner ear in Ai14 mice View Associated:
Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter) - Experiment - [In Vivo] Matched on: Name -> Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 experimentName -> Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 Asokan Aravind A dual vector strategy was employed: one delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (both single stranded AAV cassettes). This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice. A total dose of 4e12vg was injected into each mouse and vectors mixed in a 1:4 ratio of cas9 to guide RNA (1e12vg of CMV Sacas9 vector and 3e12vg of the sgRNA vector) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart. View Associated:
[] Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear - Experiment - [In Vivo] Matched on: Name -> Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 description -> Delivery of CRISPR/Cas9 editor via bioreducible lipid nanoparticle to the inner ear in Ai14 mice generatedDescription -> Delivery of CRISPR/Cas9 editor via bioreducible lipid nanoparticle to the inner ear in Ai14 mice ..SpCas9 experimentName -> Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 Murray Stephen A Delivery of CRISPR/Cas9 editor via bioreducible lipid nanoparticle to the inner ear in Ai14 mice View Associated:
[] Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides - Experiment - [In Vivo] Matched on: Name -> Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using description -> Ribonucleoproteins for CRISPR/Cas9 editing are complexed with amphiphilic peptides for delivery to lung generatedDescription -> Ribonucleoproteins for CRISPR/Cas9 editing are complexed with amphiphilic peptides for delivery to lung ; transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated ; transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated experimentName -> Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Heaney Jason D Ribonucleoproteins for CRISPR/Cas9 editing are complexed with amphiphilic peptides for delivery to lung airway epithilia via intranasal instillation into mTmG reporter mice. Editing is detected by production of GFP protein, and green fluorescence in airway linings View Associated:
Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems - Study Matched on: study -> Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems Name -> Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems Doudna Jennifer A Date Of Submission: 2020-10-16 View Associated:
[] Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain - Experiment - [In Vivo] Matched on: Name -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to description -> Delivery of CRISPR/Cas9 via RNP-loaded nanocages to the brain in Ai14 mice generatedDescription -> Delivery of CRISPR/Cas9 via RNP-loaded nanocages to the brain in Ai14 mice..SpCas9 with N- and C-terminal experimentName -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to Murray Stephen A Delivery of CRISPR/Cas9 via RNP-loaded nanocages to the brain in Ai14 mice View Associated:
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) - Experiment - [In Vivo] Matched on: Name -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to Asokan Aravind A dual vector strategy was employed: one delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (both single stranded AAV cassettes). This strategy was evaluted with both AAV9 (n=3) and AAVcc47 (n=3) by intravenous injection in Ai9 mice. A total dose of 3e12vg was injected into each mouse (1.5e12vg each vector mixed 1:1) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart. View Associated:
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter) - Experiment - [In Vivo] Matched on: Name -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to Asokan Aravind A dual vector strategy was employed: one delivering a single guide RNA and CB driven SaCas9, and another delivering the second guide RNA and CB driven SaCas9. This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice. A total dose of 2e12vg was injected into each mouse (1e12vg each vector mixed 1:1) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart. View Associated:
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector. - Experiment - [In Vivo] Matched on: Name -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to Asokan Aravind A dual vector strategy was employed: one self complementary vector delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (single stranded vector). This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=4) by intravenous injection in Ai9 mice. A total dose of 4e12vg was injected into each mouse and vectors mixed in a 1:1 ratio of cas9 to guide RNA (2e12vg of CMV Sacas9 vector and 2e12vg of the self complementary sgRNA vector) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart. View Associated:
D10 - Delivery System - [In Vivo] Matched on: generatedDescription -> transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Name -> Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using experimentName -> Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using View Associated:
D237 - Delivery System - [In Vivo] Matched on: generatedDescription -> transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Name -> Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using experimentName -> Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using View Associated:
RNP-NC-CPP - Delivery System - [In Vivo] Matched on: study -> Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Name -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to ; Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. experimentName -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to ; Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a cell penetrating peptide (CPP) from the TAT peptide (GRKKRRQRRRPQ) which lacks cell-type specficity View Associated: The Jackson Laboratory Gene Editing Testing Center - Gong Validation (Gong, Shaoqin) Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
STS159 (mTmG; Sp_t2:Sp_c0_mTmG) - Guide - [In Vivo] Matched on: study -> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription -> epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Targets mTmG and related reporter transgenes View Associated:
SpyCas9 g-loxP2_C9 - Guide - [In Vivo] Matched on: generatedDescription -> transgene......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Name -> Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using experimentName -> Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using This sgRNA targets the mTmG transgene View Associated:
Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain - Experiment - [In Vivo] Matched on: study -> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription -> epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated ; epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Sontheimer Erik J Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged. View Associated:
[] Validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing. - Experiment - [In Vivo] Matched on: description -> Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery. generatedDescription -> Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery. Heaney Jason D Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery. Detection of editing in non-target tissues. View Associated:
Testing newly chemically modified crRNA and tracrRNA in mTmG mouse embryonic fibroblasts - Experiment - [In Vitro] Matched on: study -> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription -> cell line..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated ; cell line..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Sontheimer Erik J Chemically modified crRNA and tracrRNA were delivered by electroporation to embryonic fibroblasts harvested from the mTmG reporter mouse. Gene editing was determined by reporter activation. View Associated:
Ai14 gRNA - Guide - [In Vivo] Matched on: study -> Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Name -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to ; Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. experimentName -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to ; Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. This sgRNA targets the Ai9 and related transgenes at multiple sites View Associated: The Jackson Laboratory Gene Editing Testing Center - Gong Validation (Gong, Shaoqin) Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain - Experiment - [In Vivo] Matched on: study -> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription -> transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated ; transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Sontheimer Erik J Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged. View Associated:
RNP-NC-no ligand - Delivery System - [In Vivo] Matched on: study -> Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Name -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to ; Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. experimentName -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to ; Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. View Associated: The Jackson Laboratory Gene Editing Testing Center - Gong Validation (Gong, Shaoqin) Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain - Experiment - [In Vivo] Matched on: study -> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription -> transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated ; transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Sontheimer Erik J Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged. View Associated:
AB_1196615 - Antibody - [In Vivo] Matched on: study -> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription -> locus......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated GFP (D5.1) XP Rabbit mAb antibody, Cell Signaling Technology View Associated:
SpCas9 - Genome Editor Matched on: generatedDescription -> NHEJ repair ..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Name -> Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using experimentName -> Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using View Associated:
sg298 - Guide - [In Vivo] Matched on: Name -> Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 ; Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear experimentName -> Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 ; Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear This sgRNA targets the Ai9 and related transgenes at multiple sites. 2'-O-Methyl at 3 first and last bases, 3' phosphorothioate bonds between first 3 and last 2 bases View Associated: Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain - Experiment - [In Vivo] Matched on: study -> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription -> transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated ; transgenes......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Sontheimer Erik J Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged. View Associated:
Mouse Embryonic Fibroblasts - Model System - [In Vitro] Matched on: study -> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription -> cell line..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Primary cell line View Associated:
sNLS-SpCas9-sNLS - Genome Editor Matched on: study -> Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Name -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to ; Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. experimentName -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to ; Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. SpCas9 with N- and C-terminal SV40 NLS View Associated: The Jackson Laboratory Gene Editing Testing Center - Gong Validation (Gong, Shaoqin) Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
AAVcc47-Ai9-sgRNA1 + sgRNA2 - Vector Matched on: Name -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus View Associated: Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter)
[] Second site validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain - Experiment - [In Vivo] Matched on: description -> Validation of delivery of AAV custom designed to cross the blood-brain barrier for CRISPR/Cas9 editing generatedDescription -> Validation of delivery of AAV custom designed to cross the blood-brain barrier for CRISPR/Cas9 editing Heaney Jason D Validation of delivery of AAV custom designed to cross the blood-brain barrier for CRISPR/Cas9 editing. Editing detected and quantified in brain by generation of tdTomato fluorescent protein signal from Ai9 reporter mice View Associated:
RNP-NC-RVG - Delivery System - [In Vivo] Matched on: Name -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to experimentName -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a RVG peptide YTIWMPENPRPGTPCDIFTNSRGKRASNG which specifically interacts withthe N-acetylecholine receptor (AchR) on neuronal cells, which mediates NP entry View Associated:
AB_10711040 - Antibody - [In Vivo] Matched on: Name -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to experimentName -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to Abcam, mouse anti-NeuN View Associated:
AB_2813835 - Antibody - [In Vivo] Matched on: Name -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to experimentName -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to Abcam, Donkey anti-rat alexa fluour 647 View Associated:
Ai14 mouse - Model System - [In Vivo] Matched on: study -> Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Name -> Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. experimentName -> Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain. View Associated:
AB_2209751 - Antibody - [In Vivo] Matched on: study -> Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases Name -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to experimentName -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to Anti-RFP (RABBIT) Antibody View Associated: Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases The Jackson Laboratory Gene Editing Testing Center - Gong Validation (Gong, Shaoqin) Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Testing AAV5 for activation of tdTomato in mouse airway
CleanCap® Cas9 mRNA (5moU) - Genome Editor Matched on: Name -> Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 ; Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear experimentName -> Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 ; Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear SpCas9 mRNA with 2 NLS signals, HA tag and capped using CleanCap. It is polyadenylated, substituted with a modified uridine and optimized for mammalian systems. It mimics a fully processed mature mRNA. View Associated: Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
AAV9-CMV-SaCas9 - Vector Matched on: Name -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 AAV serotype 9 delivering CMV driven SaCas9 View Associated: Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.
AAV9-Ai9-sgRNA1-CB-SaCas9 - Vector Matched on: Name -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to AAV serotype 9 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus View Associated:
AAV9-Ai9-sgRNA2-CB-SaCas9 - Vector Matched on: Name -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to AAV serotype 9 delivering gRNA 2 + CB SaCas9 targeting the Ai9 locus View Associated:
AAVcc47-Ai9-sgRNA1-CB-SaCas9 - Vector Matched on: Name -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to AAVcc47 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus View Associated:
AAVcc47_pTR_self comp 2xU6-Ai9 guides - Vector Matched on: Name -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to AAVcc47 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 transgene View Associated:
Testing preparation for validation at The Jackson Laboratory Small Animal Testing Center - Experiment - [In Vivo] Matched on: study -> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription -> locus......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated ; locus......mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Sontheimer Erik J Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain View Associated:
SaCas9-Lagor - Genome Editor Matched on: Name -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 View Associated: Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter) Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.
AB_2532994 - Antibody - [In Vivo] Matched on: Name -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to experimentName -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to Thermo-Fisher, Rat anti-GFAP View Associated:
AB_141637 - Antibody - [In Vivo] Matched on: Name -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to experimentName -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to Invitrogen, Donkey anti-rabbit alexa fluor 594 View Associated:
AB_141607 - Antibody - [In Vivo] Matched on: Name -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to experimentName -> Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to Invitrogen, Donkey anti-mouse alexafluor 488 View Associated:
Ai14 mouse (congenic) - Model System - [In Vivo] Matched on: Name -> Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 ; Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to ; Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear experimentName -> Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 ; Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to ; Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain. View Associated: Efficient In Vivo RNP-Based Gene Editing in the Sensory Organ Inner Ear Using Bioreducible Lipid Nanoparticles The Jackson Laboratory Gene Editing Testing Center - Gong Validation (Gong, Shaoqin) The Jackson Laboratory Gene Editing Testing Center - Chen Validation (Chen, Zheng-Yi) Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea
Ai9 mouse - Model System - [In Vivo] Matched on: study -> Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases Name -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. View Associated: Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases Novel AAVs Engineered for Efficient and Noninvasive Cross-Species Gene Editing Throughout the Central Nervous System Evolving High Potency AAV Vectors for Neuromuscular Genome Editing BCM-Rice Resource for the Analysis of Somatic Gene Editing in Mice - Deverman Validation (Deverman, Benjamin) Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter) Testing AAV5 for activation of tdTomato in mouse airway Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) Cre Recombinase dose escalation study in Ai9 mice Second site validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.
AAV9-Ai9-sgRNA1 + sgRNA2 - Vector Matched on: Name -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus View Associated: Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter)
AAVcc47-CMV-SaCas9 - Vector Matched on: Name -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to ; Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 AAVcc47 delivering CMV driven SaCas9 View Associated: Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter)
306-O12B blank - Delivery System - [In Vivo] Matched on: Name -> Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 experimentName -> Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 Combinatorial library cationic lipid nanoparticles View Associated:
306-O12B - Delivery System - [In Vivo, In Vitro] Matched on: Name -> Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 ; Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear experimentName -> Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 ; Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Combinatorial library cationic lipid nanoparticles View Associated: Efficient In Vivo RNP-Based Gene Editing in the Sensory Organ Inner Ear Using Bioreducible Lipid Nanoparticles The Jackson Laboratory Gene Editing Testing Center - Chen Validation (Chen, Zheng-Yi) Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea Testing new LNPs (lipid nanoparticles) for delivery of Fluc mRNA in adult mice Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea
AAVcc47-Ai9-sgRNA2-CB-SaCas9 - Vector Matched on: Name -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to AAVcc47 delivering sgRNA 2 + CB SaCas9 targeting the Ai9 locus View Associated:
SpyCas9-3xNLS - Genome Editor Matched on: study -> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription -> Rosa26 locus ..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated SpyCas9-3xNLS is type II-A Cas9 from Streptococcus pyogenes strain SF370. It was expressed from pMCSG7 bacterial expressing vector and purified from Escherichia coli Rosetta DE3 strain. SpyCas9 fused to 3 NLS: C-Myc-like NLS at the N-terminal SV40 NLS and Nucleoplasmin NLS at the C-terminal View Associated: Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain Delivery of RNP containing chemically modified crRNA C20 with chemically modified tracrRNA T2-PS to determine the RNP distribution in TLR-MCV mouse brain Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
AAV9_pTR_self comp 2xU6-Ai9 guides - Vector Matched on: Name -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to experimentName -> Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 transgene View Associated:
S10 - Delivery System - [In Vivo, In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides ; Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription -> epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Shuttle peptide used to deliver reagents to airway epithelia View Associated: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Testing Shuttle Peptides ability to deliver GFP-NLS to airway epithelia. Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells. Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells Gene editing in vitro by various peptide variants delivering Cas12a in NK cells.
306-S10 - Delivery System - [In Vivo, In Vitro] Matched on: Name -> Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear experimentName -> Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear combinatorial library cationic lipid nanoparticles View Associated: Efficient In Vivo RNP-Based Gene Editing in the Sensory Organ Inner Ear Using Bioreducible Lipid Nanoparticles The Jackson Laboratory Gene Editing Testing Center - Chen Validation (Chen, Zheng-Yi) Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea Testing new LNPs (lipid nanoparticles) for delivery of Fluc mRNA in adult mice Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea
SpyCas9-3xNLS - Genome Editor Matched on: study -> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription -> epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated ; cell line..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated SpyCas9-3xNLS is type II-A Cas9 from Streptococcus pyogenes strain SF370. It was expressed from pMCSG7 bacterial expressing vector and purified from Escherichia coli Rosetta DE3 strain. SpyCas9 fused to 3 NLS: C-Myc-like NLS at the N-terminal SV40 NLS and Nucleoplasmin NLS at the C-terminal View Associated: Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Testing newly chemically modified crRNA and tracrRNA in mTmG mouse embryonic fibroblasts Testing newly chemically modified crRNA and tracrRNA to activate the TLR1 reporter in human cells Testing newly chemically modified crRNA and tracrRNA in mouse Hepa 1-6 cells Testing newly chemically modified crRNA and tracrRNA in mouse Neuro 2A cells Testing preparation for validation at The Jackson Laboratory Small Animal Testing Center Testing newly chemically modified crRNA and tracrRNA to activate the TLR reporter in human cells
Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells - Experiment - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides McCray Paul B Delivery of Cas9 RNP targeting human CFTR in Primary human epithelia cells. Gene editing efficiency was determined by percentage of NGS reads that showed an indel View Associated:
Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells. - Experiment - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides McCray Paul B In Vitro shuttle peptide delivery of Cas12a RNPs targeting human CFTR and HPRT genes in human primary airway epithelia. Editing efficiency was assessed after 72hrs by sanger sequencing. View Associated:
FSD112 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD114 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD114d1 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD116d1 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD122 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD159 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD186 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD189 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD197 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD227 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD262 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD284 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD287 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD307 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD308 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD310 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD318 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD330 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD366 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD57d5 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD63 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD97 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSX7 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
BALB/c mouse - Model System - [In Vivo] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides BALB/cJ is a commonly used inbred. Key traits include a susceptibility to developing the demyelinating disease upon infection with Theiler's murine encephalomyelitis virus. The BALB/cJ substrain is susceptible to Listeria, all species of Leishmania, and several species of Trypanosoma, but is resistant to experimental allergic orchitis (EAO). View Associated:
HEK-293T with Ai9 transient reporter assay - Model System - [In Vitro] Matched on: study -> Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases HEK-293T cells transfected with an Ai9 inducible transgene reporter plasmid used to test gene editing activity by fluorescence View Associated:
mTmG mouse (congenic) - Model System - [In Vivo] Matched on: study -> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors generatedDescription -> epithelia..mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated Name -> Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using experimentName -> Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using mTmG is a double-fluorescent reporter transgenic mouse which expresses membrane-targeted tdTomato flanked by loxP sequences, followed by membrane-targeted GFP. After genomic cleavage by Cas9 at two sites, or Cre recombinase between loxP sites, tdTomato expression is lost and GFP is expressed. View Associated: BCM-Rice Resource for the Analysis of Somatic Gene Editing in Mice - McCray Validation (McCray Jr., Paul) Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain Testing preparation for validation at The Jackson Laboratory Small Animal Testing Center Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
FSD117D1 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides ; Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Shuttle peptide used to deliver reagents to airway epithelia View Associated: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells Testing newly chemically modified crRNA and tracrRNA to activate the TLR reporter in human cells
Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells - Experiment - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides McCray Paul B Delivery of Cas12a RNP targeting human CFTR in Primary human epithelia cells. Gene editing efficiency was determined by percentage of NGS reads that showed an indel View Associated:
Gene editing in vitro by various peptide variants delivering Cas12a in NK cells. - Experiment - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides McCray Paul B In Vitro shuttle peptide delivery of Cas12a RNP targeting human NKG2A gene in human NK cells. Gene editing was assessed after 48hr by T7E1 digestion. View Associated:
Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia - Experiment - [In Vivo] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides McCray Paul B In vivo shuttle peptide delivery of Cas9 and Cas12a RNPs in mouse airway epithelia. Gene editing was quantified by the GFP+ cells in large and small airways following 1 delivery of GFP protein by GFP positive cells compared to DAPI stained cells. View Associated:
AsCas12a (Feldan Therapeutics) - Genome Editor Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides View Associated: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells. Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells Gene editing in vitro by various peptide variants delivering Cas12a in NK cells.
AsCas12a (IDT and Feldan Therapeutics) - Genome Editor Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides View Associated:
Cre recombinase - Genome Editor Matched on: study -> Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases Cre recombinase delivered by scAAV5-Cre-GFP plasmid View Associated:
FSD115 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD121 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD168 cyclic - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD168d10 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD168d11 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD193 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD228 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD236 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD259 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD283 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD288 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD297 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD303 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD319 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD321 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD359 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD360 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD365 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD367 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD57d1 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSX2 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSX4 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
S10D - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
S10-MOD - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
AAV.pU1a-SpCas9 - Vector Matched on: study -> Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases Expresses codon-optimized SpCas9 in mammalian cells. HA-SV40NLS-SpCas9-SV40NLS View Associated:
H509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1) - Vector Matched on: study -> Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases AAV2/5 expressing SpyCas9. AAV2/5 expressing two sgRNAs under U6 promoter and eGFP View Associated:
g-loxP2_C9 - Guide - [In Vivo] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides This sgRNA targets the Ai9 and related transgenes View Associated:
NKG2A - Guide - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides gRNA targeting human NKG2A exon 3 View Associated:
Testing newly chemically modified crRNA and tracrRNA to activate the TLR reporter in human cells - Experiment - [In Vitro] Matched on: study -> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Sontheimer Erik J Chemically modified crRNA and tracrRNA were delivered by electroporation in presence of amphiphilic peptide to transgenic human HEK-293T cells harboring the TLR-MCV1 reporter. Gene editing was determined by reporter activation. View Associated:
Testing newly chemically modified crRNA and tracrRNA in mouse Neuro 2A cells - Experiment - [In Vitro] Matched on: study -> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Sontheimer Erik J Chemically modified crRNA and tracrRNA were delivered by electroporation to mouse Neuro2A cells. Editing activity was determined by Sanger sequencing View Associated:
STS118 (TLR-MCV1a; Sp_t2:Sp_c20_TLR_MCV1a) - Guide - [In Vitro] Matched on: study -> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Targets traffic light reporter transgene View Associated:
CM18-PTD4 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide View Associated: Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells. Gene editing in vitro by various peptide variants delivering Cas12a in NK cells.
FS66d6 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD132 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD168 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD168d12 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD168d20 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide View Associated:
FSD168 Disul. - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD168 Scr. - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD190 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD199 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD238 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD286 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD289 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD291 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD301 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD315 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD332 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD335 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD363 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSD364 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSX3 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
FSX8 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide used to deliver reagents to airway epithelia View Associated: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
S18 - Delivery System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Shuttle peptide View Associated: Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells. Gene editing in vitro by various peptide variants delivering Cas12a in NK cells.
pH509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1) - Vector Matched on: study -> Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases AAV2/5 expressing SpyCas9. AAV2/5 expressing two sgRNAs under U6 promoter and eGFP View Associated:
McCray_CFTR_Cas9_Guide1 - Guide - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Targets endogenous human locus View Associated:
sgAi9L - Guide - [In Vitro] Matched on: study -> Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases This sgRNA targets the Ai9 and related transgenes View Associated:
sgAi9R - Guide - [In Vitro] Matched on: study -> Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases This sgRNA targets the Ai9 and related transgenes View Associated:
NK - Model System - [In Vitro] Matched on: study -> Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides View Associated:
Testing newly chemically modified crRNA and tracrRNA in mouse Hepa 1-6 cells - Experiment - [In Vitro] Matched on: study -> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors Sontheimer Erik J Chemically modified crRNA and tracrRNA were delivered by electroporation to mouse Hepa 1-6 cells. Editing activity was determined by Sanger sequencing View Associated:
HEK-293T-disrupted_GFP-mcherry-Puro - Model System - [In Vitro] Matched on: study -> Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors HEK293T cells with an integrated reporter for TLR1 reporter editing View Associated:

Contact SCGE

282 results for crispr

282 results for crispr

Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides - Study

Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. - Experiment - [In Vivo]

Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors - Study

Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors - Study

Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain - Study

Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases - Study

Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides - Study

[] Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear - Experiment - [In Vivo]

Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter) - Experiment - [In Vivo]

[] Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear - Experiment - [In Vivo]

[] Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides - Experiment - [In Vivo]

Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems - Study

[] Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain - Experiment - [In Vivo]

Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) - Experiment - [In Vivo]

Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter) - Experiment - [In Vivo]

Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector. - Experiment - [In Vivo]

D10 - Delivery System - [In Vivo]

D237 - Delivery System - [In Vivo]

RNP-NC-CPP - Delivery System - [In Vivo]

STS159 (mTmG; Sp_t2:Sp_c0_mTmG) - Guide - [In Vivo]

SpyCas9 g-loxP2_C9 - Guide - [In Vivo]

Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain - Experiment - [In Vivo]

[] Validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing. - Experiment - [In Vivo]

Testing newly chemically modified crRNA and tracrRNA in mTmG mouse embryonic fibroblasts - Experiment - [In Vitro]

Ai14 gRNA - Guide - [In Vivo]

Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain - Experiment - [In Vivo]

RNP-NC-no ligand - Delivery System - [In Vivo]

Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain - Experiment - [In Vivo]

AB_1196615 - Antibody - [In Vivo]

SpCas9 - Genome Editor

sg298 - Guide - [In Vivo]

Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain - Experiment - [In Vivo]

Mouse Embryonic Fibroblasts - Model System - [In Vitro]

sNLS-SpCas9-sNLS - Genome Editor

AAVcc47-Ai9-sgRNA1 + sgRNA2 - Vector

[] Second site validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain - Experiment - [In Vivo]

RNP-NC-RVG - Delivery System - [In Vivo]

AB_10711040 - Antibody - [In Vivo]

AB_2813835 - Antibody - [In Vivo]

Ai14 mouse - Model System - [In Vivo]

AB_2209751 - Antibody - [In Vivo]

CleanCap® Cas9 mRNA (5moU) - Genome Editor

AAV9-CMV-SaCas9 - Vector

AAV9-Ai9-sgRNA1-CB-SaCas9 - Vector

AAV9-Ai9-sgRNA2-CB-SaCas9 - Vector

AAVcc47-Ai9-sgRNA1-CB-SaCas9 - Vector

AAVcc47_pTR_self comp 2xU6-Ai9 guides - Vector

Testing preparation for validation at The Jackson Laboratory Small Animal Testing Center - Experiment - [In Vivo]

SaCas9-Lagor - Genome Editor

AB_2532994 - Antibody - [In Vivo]

AB_141637 - Antibody - [In Vivo]

AB_141607 - Antibody - [In Vivo]

Ai14 mouse (congenic) - Model System - [In Vivo]

Ai9 mouse - Model System - [In Vivo]

AAV9-Ai9-sgRNA1 + sgRNA2 - Vector

AAVcc47-CMV-SaCas9 - Vector

306-O12B blank - Delivery System - [In Vivo]

306-O12B - Delivery System - [In Vivo, In Vitro]

AAVcc47-Ai9-sgRNA2-CB-SaCas9 - Vector

SpyCas9-3xNLS - Genome Editor

AAV9_pTR_self comp 2xU6-Ai9 guides - Vector

S10 - Delivery System - [In Vivo, In Vitro]

306-S10 - Delivery System - [In Vivo, In Vitro]

SpyCas9-3xNLS - Genome Editor

Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells - Experiment - [In Vitro]

Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells. - Experiment - [In Vitro]

FSD112 - Delivery System - [In Vitro]

FSD114 - Delivery System - [In Vitro]

FSD114d1 - Delivery System - [In Vitro]

FSD116d1 - Delivery System - [In Vitro]

FSD122 - Delivery System - [In Vitro]

FSD159 - Delivery System - [In Vitro]

FSD186 - Delivery System - [In Vitro]

FSD189 - Delivery System - [In Vitro]

FSD197 - Delivery System - [In Vitro]

FSD227 - Delivery System - [In Vitro]

FSD262 - Delivery System - [In Vitro]