Chaikof-Associated Protocol 1_Retro-orbital administration and High throughput sequencing
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This Protocol describes in vivo administration of BE-eVLP for Pcsk9 knockdown in liver, including sequencing endpoint. |
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Chaikof-Associated Protocol 2_Off-target editing AND Primers for sequencing analysis
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This protocol describes in vivo adminstration and subsequent analysis of off-target editing in the liver. |
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Chaikof-Associated Protocol 3_Serum ELISAs
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This protocol describes in vivo adminstration and subsequent blood draws and ELISA on C57BL/6J mice. |
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Conklin_Fast-Seq AAV Protocol
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Published procedure for AAV composition measurement using fast-seq.
Maynard et al. Fast-Seq: A Simple Method for Rapid and Inexpensive Validation of Packaged Single-Stranded Adeno-Associated Viral Genomes in Academic Settings. Hum Gene Ther Methods
. 2019 Dec;30(6):195-205. doi: 10.1089/hgtb.2019.110. |
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Conklin_VectorMOD Protocol
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Published procedure for mass spectrometry measurement of rAAV capsids.
Rumachik et al. VectorMOD: Method for Bottom-Up Proteomic Characterization of rAAV Capsid Post-Translational Modifications and Vector Impurities. Front Immunol. 2021 Apr 1;12:657795. doi: 10.3389/fimmu.2021.657795. eCollection 2021. |
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Deverman_AAV Production and Administration Protocol
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Published procedures for AAV production, delivery, and tissue imaging.
Challis et al. Systemic AAV vectors for widespread and targeted gene delivery in rodents. Nat Protoc. 2019 Feb;14(2):379-414. doi: 10.1038/s41596-018-0097-3.
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Deverman_Area Based Quantification of Editing Efficiency Protocol
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Procedure for non-IHC based image quantification of editing. |
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Deverman_Comprehensive Methods
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Procedure for plasmid cloning, editing evaluation in fibroblast, AAV production and administration, tissue processing and IHC. |
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Gong_Intracranial Injection Procedure for Mice
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Procedure for intracranial delivery to mouse brain. |
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Heaney-SATC_Gao-Validation_Intratracheal Delivery of AAV in Mice
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Procedure for Intratracheal (IT) delivery of AAV in mouse lung. |
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Heaney-SATC_McCray-Validation_Intranasal Instillation in Mice Protocol
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Procedure for intranasal instillation of ribonucleoprotein/peptide complex in mice for delivery to the lung. |
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Heaney-SATC_McCray-Validation_Lung Inflation and Fixation Protocol
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Procedure for mouse lung inflation and fixation. |
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Heaney_SATC Tissue Processing, Imaging and Analysis
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Procedure for tissue preparation, imaging and analysis. |
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Murray-SATC_Gong-Validaiton_Gong Study Protocol
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Procedure for intracranial injection, immunofluorescence and imaging. |
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Murray-SATC_Gong-Validation Brain Injection in Mice Protocol
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Procedure for brain injection surgical procedure, pre- and post-operative care for mice. |
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Tsai_CHANGEseq Protocol
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Procedure for editing quantification using CHANGE-seq. |
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Tsai_T Cell Transfection Protocol
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Procedure for T cell transfection. |
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