Protocols
This page contains protocols deposited by SCGE consortium laboratories.
17 results in Protocols
Chaikof-Associated Protocol 1_Retro-orbital administration and High throughput sequencingProtocol - [In Vivo] [Delivery Systems Initiative] [Mouse]Show Experiments (1)
Pcsk9 adenine base editor efficiency in liver and nonliver tissue
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Chaikof-Associated Protocol 2_Off-target editing AND Primers for sequencing analysisProtocol - [In Vivo] [Delivery Systems Initiative] [Mouse] |
Chaikof-Associated Protocol 3_Serum ELISAsProtocol - [In Vivo] [Delivery Systems Initiative] [Mouse]Show Experiments (1)
Pcsk9 adenine base editor efficiency in liver and nonliver tissue
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Conklin_Fast-Seq AAV ProtocolProtocol |
Conklin_VectorMOD ProtocolProtocol |
Deverman_AAV Production and Administration ProtocolProtocol - [In Vitro] [Delivery Systems Initiative] [Mouse] |
Deverman_Area Based Quantification of Editing Efficiency ProtocolProtocol - [In Vivo, In Vitro] [Delivery Systems Initiative] [Mouse] |
Deverman_Comprehensive MethodsProtocol - [In Vivo, In Vitro] [Delivery Systems Initiative] [Mouse] |
Gong_Intracranial Injection Procedure for MiceProtocol - [In Vivo] [Delivery Systems Initiative] [Mouse] |
Heaney-SATC_Gao-Validation_Intratracheal Delivery of AAV in MiceProtocol - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse] |
Heaney-SATC_McCray-Validation_Intranasal Instillation in Mice ProtocolProtocol - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse] |
Heaney-SATC_McCray-Validation_Lung Inflation and Fixation ProtocolProtocol - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse] |
Heaney_SATC Tissue Processing, Imaging and AnalysisProtocol - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]Show Experiments (4)
Independent validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing.
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Murray-SATC_Gong-Validaiton_Gong Study ProtocolProtocol - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse] |
Murray-SATC_Gong-Validation Brain Injection in Mice ProtocolProtocol - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse] |
Tsai_T Cell Transfection ProtocolProtocol - [In Vitro] [Biological Systems] [Human]Show Experiments (1)
A novel human T cell platform to define biological adverse effects of genome editing
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17 results in Protocols
| Name | Description | Source | View Associated.. |
|---|---|---|---|
| Chaikof-Associated Protocol 1_Retro-orbital administration and High throughput sequencing | This Protocol describes in vivo administration of BE-eVLP for Pcsk9 knockdown in liver, including sequencing endpoint. |
Show Experiments (1)
Pcsk9 adenine base editor efficiency in liver and nonliver tissue
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| Chaikof-Associated Protocol 2_Off-target editing AND Primers for sequencing analysis | This protocol describes in vivo adminstration and subsequent analysis of off-target editing in the liver. | ||
| Chaikof-Associated Protocol 3_Serum ELISAs | This protocol describes in vivo adminstration and subsequent blood draws and ELISA on C57BL/6J mice. |
Show Experiments (1)
Pcsk9 adenine base editor efficiency in liver and nonliver tissue
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| Conklin_Fast-Seq AAV Protocol | Published procedure for AAV composition measurement using fast-seq. Maynard et al. Fast-Seq: A Simple Method for Rapid and Inexpensive Validation of Packaged Single-Stranded Adeno-Associated Viral Genomes in Academic Settings. Hum Gene Ther Methods . 2019 Dec;30(6):195-205. doi: 10.1089/hgtb.2019.110. | ||
| Conklin_VectorMOD Protocol | Published procedure for mass spectrometry measurement of rAAV capsids. Rumachik et al. VectorMOD: Method for Bottom-Up Proteomic Characterization of rAAV Capsid Post-Translational Modifications and Vector Impurities. Front Immunol. 2021 Apr 1;12:657795. doi: 10.3389/fimmu.2021.657795. eCollection 2021. | ||
| Deverman_AAV Production and Administration Protocol | Published procedures for AAV production, delivery, and tissue imaging. Challis et al. Systemic AAV vectors for widespread and targeted gene delivery in rodents. Nat Protoc. 2019 Feb;14(2):379-414. doi: 10.1038/s41596-018-0097-3. | ||
| Deverman_Area Based Quantification of Editing Efficiency Protocol | Procedure for non-IHC based image quantification of editing. | ||
| Deverman_Comprehensive Methods | Procedure for plasmid cloning, editing evaluation in fibroblast, AAV production and administration, tissue processing and IHC. | ||
| Gong_Intracranial Injection Procedure for Mice | Procedure for intracranial delivery to mouse brain. | ||
| Heaney-SATC_Gao-Validation_Intratracheal Delivery of AAV in Mice | Procedure for Intratracheal (IT) delivery of AAV in mouse lung. | ||
| Heaney-SATC_McCray-Validation_Intranasal Instillation in Mice Protocol | Procedure for intranasal instillation of ribonucleoprotein/peptide complex in mice for delivery to the lung. | ||
| Heaney-SATC_McCray-Validation_Lung Inflation and Fixation Protocol | Procedure for mouse lung inflation and fixation. | ||
| Heaney_SATC Tissue Processing, Imaging and Analysis | Procedure for tissue preparation, imaging and analysis. |
Show Experiments (4)
Independent validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing.
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| Murray-SATC_Gong-Validaiton_Gong Study Protocol | Procedure for intracranial injection, immunofluorescence and imaging. | ||
| Murray-SATC_Gong-Validation Brain Injection in Mice Protocol | Procedure for brain injection surgical procedure, pre- and post-operative care for mice. | ||
| Tsai_CHANGEseq Protocol | Procedure for editing quantification using CHANGE-seq. | ||
| Tsai_T Cell Transfection Protocol | Procedure for T cell transfection. |
Show Experiments (1)
A novel human T cell platform to define biological adverse effects of genome editing
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