17 results in Protocols

Chaikof-Associated Protocol 1_Retro-orbital administration and High throughput sequencing

Protocol  - [In Vivo] [Delivery Systems] [Mouse]
Matched Fields: category : Protocol
This Protocol describes in vivo administration of BE-eVLP for Pcsk9 knockdown in liver, including sequencing endpoint.

Chaikof-Associated Protocol 2_Off-target editing AND Primers for sequencing analysis

Protocol  - [In Vivo] [Delivery Systems] [Mouse]
Matched Fields: category : Protocol
This protocol describes in vivo adminstration and subsequent analysis of off-target editing in the liver.

Chaikof-Associated Protocol 3_Serum ELISAs

Protocol  - [In Vivo] [Delivery Systems] [Mouse]
Matched Fields: category : Protocol
This protocol describes in vivo adminstration and subsequent blood draws and ELISA on C57BL/6J mice.

Conklin_Fast-Seq AAV Protocol

Protocol 
Matched Fields: category : Protocol
Published procedure for AAV composition measurement using fast-seq. Maynard et al. Fast-Seq: A Simple Method for Rapid and Inexpensive Validation of Packaged Single-Stranded Adeno-Associated Viral Genomes in Academic Settings. Hum Gene Ther Methods . 2019 Dec;30(6):195-205. doi: 10.1089/hgtb.2019.110.

Conklin_VectorMOD Protocol

Protocol 
Matched Fields: category : Protocol
Published procedure for mass spectrometry measurement of rAAV capsids. Rumachik et al. VectorMOD: Method for Bottom-Up Proteomic Characterization of rAAV Capsid Post-Translational Modifications and Vector Impurities. Front Immunol. 2021 Apr 1;12:657795. doi: 10.3389/fimmu.2021.657795. eCollection 2021.

Deverman_AAV Production and Administration Protocol

Protocol  - [In Vitro] [Delivery Systems] [Mouse]
Matched Fields: category : Protocol
Published procedures for AAV production, delivery, and tissue imaging. Challis et al. Systemic AAV vectors for widespread and targeted gene delivery in rodents. Nat Protoc. 2019 Feb;14(2):379-414. doi: 10.1038/s41596-018-0097-3.

Deverman_Area Based Quantification of Editing Efficiency Protocol

Protocol  - [In Vivo, In Vitro] [Delivery Systems] [Mouse]
Matched Fields: category : Protocol
Procedure for non-IHC based image quantification of editing.

Deverman_Comprehensive Methods

Protocol  - [In Vivo, In Vitro] [Delivery Systems] [Mouse]
Matched Fields: category : Protocol
Procedure for plasmid cloning, editing evaluation in fibroblast, AAV production and administration, tissue processing and IHC.

Gong_Intracranial Injection Procedure for Mice

Protocol  - [In Vivo] [Delivery Systems] [Mouse]
Matched Fields: category : Protocol
Procedure for intracranial delivery to mouse brain.

Heaney-SATC_Gao-Validation_Intratracheal Delivery of AAV in Mice

Protocol  - [In Vivo] [Animal Reporter and Testing Center] [Mouse]
Matched Fields: category : Protocol
Procedure for Intratracheal (IT) delivery of AAV in mouse lung.

Heaney-SATC_McCray-Validation_Intranasal Instillation in Mice Protocol

Protocol  - [In Vivo] [Animal Reporter and Testing Center] [Mouse]
Matched Fields: category : Protocol
Procedure for intranasal instillation of ribonucleoprotein/peptide complex in mice for delivery to the lung.

Heaney-SATC_McCray-Validation_Lung Inflation and Fixation Protocol

Protocol  - [In Vivo] [Animal Reporter and Testing Center] [Mouse]
Matched Fields: category : Protocol
Procedure for mouse lung inflation and fixation.

Murray-SATC_Gong-Validaiton_Gong Study Protocol

Protocol  - [In Vivo] [Animal Reporter and Testing Center] [Mouse]
Matched Fields: category : Protocol
Procedure for intracranial injection, immunofluorescence and imaging.

Murray-SATC_Gong-Validation Brain Injection in Mice Protocol

Protocol  - [In Vivo] [Animal Reporter and Testing Center] [Mouse]
Matched Fields: category : Protocol
Procedure for brain injection surgical procedure, pre- and post-operative care for mice.

Tsai_CHANGEseq Protocol

Protocol 
Matched Fields: category : Protocol
Procedure for editing quantification using CHANGE-seq.

Tsai_T Cell Transfection Protocol

Protocol  - [In Vitro] [Biological Effects] [Human]
Matched Fields: category : Protocol
Procedure for T cell transfection.

17 results in Protocols

Name Description Source View Associated...
Chaikof-Associated Protocol 1_Retro-orbital administration and High throughput sequencing This Protocol describes in vivo administration of BE-eVLP for Pcsk9 knockdown in liver, including sequencing endpoint.
Chaikof-Associated Protocol 2_Off-target editing AND Primers for sequencing analysis This protocol describes in vivo adminstration and subsequent analysis of off-target editing in the liver.
Chaikof-Associated Protocol 3_Serum ELISAs This protocol describes in vivo adminstration and subsequent blood draws and ELISA on C57BL/6J mice.
Conklin_Fast-Seq AAV Protocol Published procedure for AAV composition measurement using fast-seq. Maynard et al. Fast-Seq: A Simple Method for Rapid and Inexpensive Validation of Packaged Single-Stranded Adeno-Associated Viral Genomes in Academic Settings. Hum Gene Ther Methods . 2019 Dec;30(6):195-205. doi: 10.1089/hgtb.2019.110.
Conklin_VectorMOD Protocol Published procedure for mass spectrometry measurement of rAAV capsids. Rumachik et al. VectorMOD: Method for Bottom-Up Proteomic Characterization of rAAV Capsid Post-Translational Modifications and Vector Impurities. Front Immunol. 2021 Apr 1;12:657795. doi: 10.3389/fimmu.2021.657795. eCollection 2021.
Deverman_AAV Production and Administration Protocol Published procedures for AAV production, delivery, and tissue imaging. Challis et al. Systemic AAV vectors for widespread and targeted gene delivery in rodents. Nat Protoc. 2019 Feb;14(2):379-414. doi: 10.1038/s41596-018-0097-3.
Deverman_Area Based Quantification of Editing Efficiency Protocol Procedure for non-IHC based image quantification of editing.
Deverman_Comprehensive Methods Procedure for plasmid cloning, editing evaluation in fibroblast, AAV production and administration, tissue processing and IHC.
Gong_Intracranial Injection Procedure for Mice Procedure for intracranial delivery to mouse brain.
Heaney-SATC_Gao-Validation_Intratracheal Delivery of AAV in Mice Procedure for Intratracheal (IT) delivery of AAV in mouse lung.
Heaney-SATC_McCray-Validation_Intranasal Instillation in Mice Protocol Procedure for intranasal instillation of ribonucleoprotein/peptide complex in mice for delivery to the lung.
Heaney-SATC_McCray-Validation_Lung Inflation and Fixation Protocol Procedure for mouse lung inflation and fixation.
Heaney_SATC Tissue Processing, Imaging and Analysis Procedure for tissue preparation, imaging and analysis.
Murray-SATC_Gong-Validaiton_Gong Study Protocol Procedure for intracranial injection, immunofluorescence and imaging.
Murray-SATC_Gong-Validation Brain Injection in Mice Protocol Procedure for brain injection surgical procedure, pre- and post-operative care for mice.
Tsai_CHANGEseq Protocol Procedure for editing quantification using CHANGE-seq.
Tsai_T Cell Transfection Protocol Procedure for T cell transfection.