308 results for CRISPR

Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides

Project  [Delivery Systems Initiative]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides name -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B  Last Updated Date: 2020-11-02 NIH Report
 
The proposed research is relevant to the public health because genetic and acquired diseases affecting the airways pose major disease and economic burdens. By advancing the delivery of gene editing tools, it may be possible to therapeutically modify the cells lining the airways. This novel strategy has implications for the treatment of both monogenetic and acquired lungs disease, and may have applications for other somatic cell therapies.

Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors

Project  [Delivery Systems Initiative]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors  -->  RNA-guided CRISPR genome editing systems promise to revolutionize the treatment of inherited disease.  that directs editing is a critical hurdle in the development of clinical applications for engineered CRISPR  of nucleic acid therapeutics, we have established a framework for complete chemical modification of CRISPR name -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J  Last Updated Date: 2021-04-15 NIH Report
 
RNA-guided CRISPR genome editing systems promise to revolutionize the treatment of inherited disease. Safe, effective, and target-tissue-specific delivery of the guide RNA that directs editing is a critical hurdle in the development of clinical applications for engineered CRISPR systems. Using strategies validated for the delivery of other categories of nucleic acid therapeutics, we have established a framework for complete chemical modification of CRISPR guides, thereby conferring in vivo stability and effective biodistribution properties. The proposed research will optimize these guides, as well as other editing components, for clinical use.
Show Experiments (11)

Testing newly chemically modified crRNA and tracrRNA in mTmG mouse embryonic fibroblasts
Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
Testing newly chemically modified crRNA and tracrRNA to activate the TLR1 reporter in human cells
Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
Testing newly chemically modified crRNA and tracrRNA in mouse Hepa 1-6 cells
Delivery of RNP containing chemically modified crRNA C20 with chemically modified tracrRNA T2-PS to determine the RNP distribution in TLR-MCV mouse brain
Testing newly chemically modified crRNA and tracrRNA in mouse Neuro 2A cells
Testing preparation for independent validation at The Jackson Laboratory Small Animal Testing Center
Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain
Testing newly chemically modified crRNA and tracrRNA to activate the TLR reporter in human cells
Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain

Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.

Experiment  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain  -->  Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.  -->  Nanocapusules carrying CRISPR Cas9 RNP with guide RNA targeting the stop sequence in the Ai14 transgene name -->  Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.  -->  Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
Gong Shaoqin (Sarah)  Last Updated Date: 2020-10-28
 
Nanocapusules carrying CRISPR Cas9 RNP with guide RNA targeting the stop sequence in the Ai14 transgene are intracerebrally delivered to Ai14 mice and gene editing is measured by gain of tdTomato protein expression.

Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases

Project  [Delivery Systems Initiative]
Matched on:  -->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases name -->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
Gao Guang-Ping  Last Updated Date: 2020-10-20 NIH Report
 
Efficacy and safety limitations in current gene editing technologies have hindered efforts to treat genetic lung diseases. This proposal seeks to develop and validate a combinatorial delivery approach that uses non-viral and viral vehicles to efficiently transport gene editing tools to disease-relevant cells in the lung. Completion of our work will establish safe and effective delivery vehicles that will guide the design of future gene therapies for genetic disorders.

Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain

Project  [Delivery Systems Initiative]
Matched on:  -->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain  -->  In vivo genome editing using CRISPR/Cas9 is anticipated to be the next wave of therapeutics for various name -->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain
Gong Shaoqin (Sarah)  Last Updated Date: 2020-10-28 NIH Report
 
In vivo genome editing using CRISPR/Cas9 is anticipated to be the next wave of therapeutics for various major health threats, including neurodegenerative diseases. However, to date, very few Cas9-gRNA ribonucleoprotein in vivo delivery methods have been reported, and delivery to the brain has been particularly challenging. The unique nanocapsules we plan to develop will ultimately enable high efficiency neuron-targeted genome editing in the brain, thereby offering new hope to treat devastating neurodegenerative diseases.

[Validation] Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain

Experiment  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to  -->  Delivery of CRISPR/Cas9 via RNP-loaded nanocages to the brain in Ai14 mice name -->  Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to  -->  Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Murray Stephen A  Last Updated Date: 2023-05-10
 
Delivery of CRISPR/Cas9 via RNP-loaded nanocages to the brain in Ai14 mice

[Validation] Repeat experiment of independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear

Experiment  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Repeat experiment of independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9  -->  Delivery of CRISPR/Cas9 editor via bioreducible lipid nanoparticle to the inner ear in Ai14 mice name -->  Repeat experiment of independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9  -->  Repeat experiment of independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9
Murray Stephen A  Last Updated Date: 2023-05-10
 
Delivery of CRISPR/Cas9 editor via bioreducible lipid nanoparticle to the inner ear in Ai14 mice

Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter)

Experiment  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to name -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
Asokan Aravind  Last Updated Date: 2021-09-16
 
A dual vector strategy was employed: one delivering a single guide RNA and CB driven SaCas9, and another delivering the second guide RNA and CB driven SaCas9. This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice. A total dose of 2e12vg was injected into each mouse (1e12vg each vector mixed 1:1) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.

[Validation] Independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear

Experiment  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear  -->  Delivery of CRISPR/Cas9 via bioreducible lipid nanoparticles (LNPs) to the inner ear in Ai14 mice name -->  Independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear  -->  Independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
Murray Stephen A  Last Updated Date: 2023-05-10
 
Delivery of CRISPR/Cas9 via bioreducible lipid nanoparticles (LNPs) to the inner ear in Ai14 mice

Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems

Project  [Genome Editors]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems  -->  Expanding genome editing tools through exploration of new CRISPR-Cas proteins and DNA repair enzymes  NARRATIVE Fundamental research on bacterial adaptive immunity uncovered the genome editing properties of CRISPR-Cas  We will focus our investigation on newly described CRISPR-Cas systems and DNA-interacting proteins that name -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Banfield Jillian , Doudna Jennifer A  Last Updated Date: 2020-10-16 NIH Report
 
Expanding genome editing tools through exploration of new CRISPR-Cas proteins and DNA repair enzymes NARRATIVE Fundamental research on bacterial adaptive immunity uncovered the genome editing properties of CRISPR-Cas systems, and it is clear that uncultivated microbes contain more pathways and enzymes that may be useful as genome editing tools. We will combine bioinformatics and biochemistry to identify new DNA- and RNA-associating proteins and will analyze their mechanisms of action. We will focus our investigation on newly described CRISPR-Cas systems and DNA-interacting proteins that occur in conserved genomic context.

[Validation] Independent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides

Experiment  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Independent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia  -->  Ribonucleoproteins for CRISPR/Cas9 editing are complexed with amphiphilic peptides for delivery to lung name -->  Independent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia  -->  Independent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia
Heaney Jason D  Last Updated Date: 2021-09-07
 
Ribonucleoproteins for CRISPR/Cas9 editing are complexed with amphiphilic peptides for delivery to lung airway epithilia via intranasal instillation into mTmG reporter mice. Editing is detected by production of GFP protein, and green fluorescence in airway linings

Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter)

Experiment  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 name -->  Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9  -->  Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
Asokan Aravind  Last Updated Date: 2021-09-16
 
A dual vector strategy was employed: one delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (both single stranded AAV cassettes). This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice. A total dose of 4e12vg was injected into each mouse and vectors mixed in a 1:4 ratio of cas9 to guide RNA (1e12vg of CMV Sacas9 vector and 3e12vg of the sgRNA vector) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.

Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter)

Experiment  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to name -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to
Asokan Aravind  Last Updated Date: 2021-09-16
 
A dual vector strategy was employed: one delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (both single stranded AAV cassettes). This strategy was evaluted with both AAV9 (n=3) and AAVcc47 (n=3) by intravenous injection in Ai9 mice. A total dose of 3e12vg was injected into each mouse (1.5e12vg each vector mixed 1:1) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.

Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.

Experiment  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to name -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
Asokan Aravind  Last Updated Date: 2021-09-16
 
A dual vector strategy was employed: one self complementary vector delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (single stranded vector). This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=4) by intravenous injection in Ai9 mice. A total dose of 4e12vg was injected into each mouse and vectors mixed in a 1:1 ratio of cas9 to guide RNA (2e12vg of CMV Sacas9 vector and 2e12vg of the self complementary sgRNA vector) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart.

[Validation] Independent validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing.

Experiment  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery.
Heaney Jason D  Last Updated Date: 2021-03-30
 
Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery. Detection of editing in non-target tissues.

Ai14 gRNA

Guide  - [In Vivo] [Small Animal Testing Center (SATC), Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain  -->  Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to  Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
This sgRNA targets the Ai9 and related transgenes at multiple sites

Gong_Intracranial Injection Procedure for Mice

Protocol  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain  -->  Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
Procedure for intracranial delivery to mouse brain.

sg298

Guide  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Repeat experiment of independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9  Independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
This sgRNA targets the Ai9 and related transgenes at multiple sites. 2'-O-Methyl at 3 first and last bases, 3' phosphorothioate bonds between first 3 and last 2 bases

RNP-NC-CPP

Delivery System  - [In Vivo] [Small Animal Testing Center (SATC), Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain  -->  Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to  Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a cell penetrating peptide (CPP) from the TAT peptide (GRKKRRQRRRPQ) which lacks cell-type specficity

RNP-NC-no ligand

Delivery System  - [In Vivo] [Small Animal Testing Center (SATC), Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain  -->  Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to  Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA.

D237

Delivery System  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Independent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia

Murray-SATC_Gong-Validation Brain Injection in Mice Protocol

Protocol  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Procedure for brain injection surgical procedure, pre- and post-operative care for mice.

AB_10711040 

Antibody  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Other Id: ab104224Ā  
Abcam, mouse anti-NeuN

AB_141637 

Antibody  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Other Id: A21207 
Invitrogen, Donkey anti-rabbit alexa fluor 594

AB_141607 

Antibody  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Other Id: A21202 
Invitrogen, Donkey anti-mouse alexafluor 488

Ai14 mouse

Model System  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain  -->  Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain.

306-O12B blank

Delivery System  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Repeat experiment of independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9
Combinatorial library cationic lipid nanoparticles

D10

Delivery System  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Independent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia

Murray-SATC_Gong-Validaiton_Gong Study Protocol

Protocol  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Procedure for intracranial injection, immunofluorescence and imaging.

AB_2813835 

Antibody  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Other Id: ab150155 
Abcam, Donkey anti-rat alexa fluour 647

sNLS-SpCas9-sNLS

Genome Editor  - [In Vivo] [Small Animal Testing Center (SATC), Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain  -->  Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to  Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.
SpCas9 with N- and C-terminal SV40 NLS

SaCas9-Lagor

Genome Editor  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to  Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9

[Validation] Independent validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain

Experiment  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Validation of delivery of AAV custom designed to cross the blood-brain barrier for CRISPR/Cas9 editing
Heaney Jason D  Last Updated Date: 2023-05-10
 
Validation of delivery of AAV custom designed to cross the blood-brain barrier for CRISPR/Cas9 editing. Editing detected and quantified in brain by generation of tdTomato fluorescent protein signal from Ai9 reporter mice

AAVcc47-Ai9-sgRNA1-CB-SaCas9

Vector  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAVcc47 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus

AAVcc47-Ai9-sgRNA1 + sgRNA2

Vector  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to  Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus

AAVcc47-CMV-SaCas9

Vector  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to  Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
AAVcc47 delivering CMV driven SaCas9

CleanCapĀ® Cas9 mRNA (5moU)

Genome Editor  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Repeat experiment of independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9  Independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
SpCas9 mRNA with 2 NLS signals, HA tag and capped using CleanCap. It is polyadenylated, substituted with a modified uridine and optimized for mammalian systems. It mimics a fully processed mature mRNA.

AAV9-Ai9-sgRNA2-CB-SaCas9

Vector  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAV serotype 9 delivering gRNA 2 + CB SaCas9 targeting the Ai9 locus

RNP-NC-RVG

Delivery System  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a RVG peptide YTIWMPENPRPGTPCDIFTNSRGKRASNG which specifically interacts withthe N-acetylecholine receptor (AchR) on neuronal cells, which mediates NP entry

Heaney-SATC_McCray-Validation_Intranasal Instillation in Mice Protocol

Protocol  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Independent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia
Procedure for intranasal instillation of ribonucleoprotein/peptide complex in mice for delivery to the lung.

AB_2209751 

Antibody  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Other Id: Rockland Cat# 600-401-379 
Anti-RFP (RABBIT) Antibody

AAV9-Ai9-sgRNA1 + sgRNA2

Vector  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to  Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus

AAV9_pTR_self comp 2xU6-Ai9 guides

Vector  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 transgene

AAVcc47_pTR_self comp 2xU6-Ai9 guides

Vector  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAVcc47 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 transgene

AAVcc47-Ai9-sgRNA2-CB-SaCas9

Vector  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to
AAVcc47 delivering sgRNA 2 + CB SaCas9 targeting the Ai9 locus

SpCas9

Genome Editor  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Independent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia

306-O12B

Delivery System  - [In Vivo, In Vitro] [Small Animal Testing Center (SATC), Delivery Systems Initiative] [Mouse]
Matched on:  -->  Repeat experiment of independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9  Independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
Combinatorial library cationic lipid nanoparticles

Ai9 mouse

Model System  - [In Vivo] [Small Animal Testing Center (SATC), AAV tropism, Delivery Systems Initiative] [Mouse]
Matched on:  -->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to  Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus.
Show Experiments (10)

Testing gRNA sequence and gRNA scaffold modified in Ai9 mice.
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter)
Testing AAV5 for activation of tdTomato in mouse airway
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter)
AAV Tropism project
Cre Recombinase dose escalation study in Ai9 mice
Independent validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain
Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter)
Testing AAV5 for activation of tdTomato in mouse airway club and ciliated cells
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.

306-S10

Delivery System  - [In Vivo, In Vitro] [Small Animal Testing Center (SATC), Delivery Systems Initiative] [Mouse]
Matched on:  -->  Independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
combinatorial library cationic lipid nanoparticles

Heaney_SATC Tissue Processing, Imaging and Analysis

Protocol  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Independent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia
Procedure for tissue preparation, imaging and analysis.

FSD117D1

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Shuttle peptide used to deliver reagents to airway epithelia

Gene editing in vitro by various peptide variants delivering Cas12a in NK cells.

Experiment  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B  Last Updated Date: 2020-11-02
 
In Vitro shuttle peptide delivery of Cas12a RNP targeting human NKG2A gene in human NK cells. Gene editing was assessed after 48hr by T7E1 digestion.

Testing AAV5 for activation of tdTomato in HEK293T cells

Experiment  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
Gao Guang-Ping  Last Updated Date: 2020-10-20
 
AAV shuttle plasmids expressing SpCas9 and guide RNAs targeting the Ai9 transgene were tested in HEK293T cells by transient transfection. Both delivery and gene editing were detected by fluorescence.

FS48

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD118

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD168d10

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD168d20

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide

FSD168D20

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD168 Disul.

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD168 Scr.

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD216

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD234

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD260

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD288

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD293

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD315

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD329

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD333

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD364

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD57d5

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSX2

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSX6

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSX8

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

3849 45-5'

Guide  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Targets endogenous human locus

g-45_C12a

Guide  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
gRNA targeting CFTR inton-22-23

g-loxPbot_C12a

Guide  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
This sgRNA targets the Ai9 and related transgenes at two sites

sgAi9L

Guide  - [In Vivo, In Vitro] [Delivery Systems Initiative] [Human, Mouse]
Matched on:  -->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
This sgRNA targets the Ai9 and related transgenes

Antibody  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
Other Id:   Rockland Cat# 600-401-379 
Anti-RFP (RABBIT) Antibody

Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia

Experiment  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B  Last Updated Date: 2020-11-02
 
In vivo shuttle peptide delivery of Cas9 and Cas12a RNPs in mouse airway epithelia. Gene editing was quantified by the GFP+ cells in large and small airways following 1 delivery of GFP protein by GFP positive cells compared to DAPI stained cells.

Cre recombinase

Genome Editor  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
Cre recombinase delivered by plasmid (see vector details)

CM18-PTD4

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide

FSD112

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD114d1

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD168d11

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD190

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD235

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD237

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD284

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD285

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD289

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD303

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD304

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD310

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD321

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD339

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD341

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD362

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD365

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD367

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD368

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD57d3

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD57d6

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD63

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD63D1

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSX3

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

pAAV.pU1a-SpCas9

Vector  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
Expresses codon-optimized SpCas9 in mammalian cells. HA-SV40NLS-SpCas9-SV40NLS

g-11_C9

Guide  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
sgRNA targeting CFTR exon 11

g-38330_C12a

Guide  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
gRNA targeting HPRT locus

g-loxP2_C9

Guide  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
This sgRNA targets the Ai9 and related transgenes

mTmG mouse

Model System  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
ROSAmT/mGĀ is a cell membrane-targeted, two-color fluorescent Cre-reporter allele. Prior to Cre recombination, cell membrane-localized tdTomato (mT) fluorescence expression is widespread in cells/tissues. Cre recombinase expressing cells (and future cell lineages derived from these cells) have cell membrane-localized EGFP (mG) fluorescence expression replacing the red fluorescence

Primary Airway Epithelia (Human)

Model System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Primary airway epithelia from non-CF donors

Antibody  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
Other Id:   Santa Cruz, SC-25555 
Anti-CC10 (Rabbit) Polyclonal Antibody, dilution used 1:2,000

Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells.

Experiment  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B  Last Updated Date: 2020-11-02
 
In Vitro shuttle peptide delivery of Cas12a RNPs targeting human CFTR and HPRT genes in human primary airway epithelia. Editing efficiency was assessed after 72hrs by sanger sequencing.

Testing AAV5 for activation of tdTomato in mouse airway

Experiment  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
Gao Guang-Ping  Last Updated Date: 2020-10-20
 
AAV2/5 mediated gene editing in the mouse airway was tested by deliverying SpCas9 and guide RNAs targeting the Ai9 transgene in Ai9 transgenic mice. Viral delivery was detected by GFP expression and gene editing quantified by tdTomato activation

Alt-RĀ® S.p. Cas9 Nuclease V3

Genome Editor  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Recombinant S. pyogenes Cas9 nuclease, purified from an E. coli strain expressing the nuclease. Contains nuclear localization sequence (NLS) and C-terminal 6-His tag. Provided in solution at 10 Āµg/ĀµL. 100 Āµg of Cas9 nuclease = 610 pmol.

SpCas9 (Feldan Therapeutics)

Genome Editor  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides

FS66d6

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD115d1

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD116d1

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD159

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD160

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD168d12

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD168d17

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD189

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD191

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD195

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD222

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD227

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD228

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD236

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD238

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD239

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD240

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD287

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD305

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD331

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD347

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD359

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD363

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD67

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD96

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSX1

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSX5

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSX7

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

S10D

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

S85

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide

sgAi9R

Guide  - [In Vivo, In Vitro] [Delivery Systems Initiative] [Human, Mouse]
Matched on:  -->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
This sgRNA targets the Ai9 and related transgenes

BALB/c mouse

Model System  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
BALB/cJ is a commonly used inbred. Key traits include a susceptibility to developing the demyelinating disease upon infection with Theiler's murine encephalomyelitis virus. The BALB/cJ substrain is susceptible to Listeria, all species of Leishmania, and several species of Trypanosoma, but is resistant to experimental allergic orchitis (EAO).

HEK-293T with Ai9 transient reporter assay

Model System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
HEK-293T cells transfected with an Ai9 inducible transgene reporter plasmid used to test gene editing activity by fluorescence. HEK293T is an epithelial-like cell that was isolated from the kidney of a patient.

NK

Model System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Natural Killer cells. White blood cells;

Antibody  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
Other Id:   Thermo Fisher Scientific, MA5-15257 
Anti-RFP (Mouse) Monoclonal Antibody, dilution used 1:300

Testing Shuttle Peptides ability to deliver GFP-NLS to airway epithelia.

Experiment  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B  Last Updated Date: 2020-11-02
 
Delivery of GFP via shuttle peptides to mouse airway epithelium via nasal instilation. Delivery efficiency was quantified in large and small airways by counting the number of GFP positive cells divided by the number of DAPI cells.

FSD121

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD168 cyclic

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD193

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD196

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD199

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD215

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD259

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD262

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD283

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD291

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD301

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD306

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD307

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD308

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD316

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD322

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD334

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD366

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD57

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD97

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSX4

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

S18

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide

H509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1)

Vector  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
AAV2/5 expressing SpyCas9. AAV2/5 expressing two sgRNAs under U6 promoter and eGFP

pH509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1)

Vector  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
AAV2/5 expressing SpyCas9. AAV2/5 expressing two sgRNAs under U6 promoter and eGFP

McCray_CFTR_Cas9_Guide1

Guide  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Targets endogenous human locus

Antibody  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
Other Id:   Santa Cruz, SC-23950 
Anti-alpha Tubulin (Mouse) Monoclonal Antibody, dilution used 1:200

Antibody  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
Other Id:   Rockland, 600- 401-379 
Anti-RFP (Rabbit) Polyclonal Antibody

S10

Delivery System  - [In Vivo, In Vitro] [Delivery Systems Initiative] [Human, Mouse]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Shuttle peptide used to deliver reagents to airway epithelia

Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain

Experiment  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J  Last Updated Date: 2021-04-15
 
Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged.

Testing newly chemically modified crRNA and tracrRNA in mouse Neuro 2A cells

Experiment  - [In Vitro] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J  Last Updated Date: 2021-04-15
 
Chemically modified crRNA and tracrRNA were delivered by electroporation to mouse Neuro2A cells. Editing activity was determined by Sanger sequencing

STS118 (TLR-MCV1a; Sp_t2-PS:Sp_c20_TLR_MCV1a)

Guide  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets traffic light reporter transgene, CY3 labeled

STS120 (TLR1; Sp_t2:Sp_c20_TLR1)

Guide  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets traffic light reporter transgene

STS204 (DNMT1; Sp_t2:Sp_c20_Dnmt1)

Guide  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
For endogenous locus

HEK-293T-disrupted_GFP-mcherry-Puro

Model System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
HEK293T cells with an integrated reporter for TLR1 reporter editing. HEK293T is an epithelial-like cell that was isolated from the kidney of a patient.

Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain

Experiment  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J  Last Updated Date: 2021-04-15
 
Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged.

Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain

Experiment  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J  Last Updated Date: 2021-04-15
 
Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged.

STS119 (TLR-MCV1b; Sp_t2:Sp_c20_TLR_MCV1b)

Guide  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets traffic light reporter transgene

STS134 (PCSK9b; Sp_t2:Sp_c20_PCSK9b)

Guide  - [In Vitro] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets endogenous mouse locus

STS135 (PCSK9c; Sp_t2:Sp_c20_PCSK9c)

Guide  - [In Vitro] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets endogenous mouse locus

STS159 (mTmG; Sp_t2-PS:Sp_c20_mTmG)

Guide  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets mTmG and related reporter transgenes

STS159 (mTmG; Sp_t2:Sp_c0_mTmG)

Guide  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets mTmG and related reporter transgenes

STS204 (DNMT1; Sp_t41:Sp_c20_Dnmt1)

Guide  - [In Vitro] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets endogenous mouse locus

Mouse Embryonic Fibroblasts

Model System  - [In Vitro] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Primary cell line

Lipofectamine 3000

Delivery System  - [In Vitro] [Genome Editors, Delivery Systems Initiative] [Human]
Matched on:  -->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Lipid nanoparticle

Testing preparation for independent validation at The Jackson Laboratory Small Animal Testing Center

Experiment  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J  Last Updated Date: 2021-10-01
 
Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain

STS118 (TLR-MCV1a; Sp_t2:Sp_c20_TLR_MCV1a)

Guide  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets traffic light reporter transgene

HEK-293T-disrupted_GFP with MCV-mcherry-Puro

Model System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
HEK293T cells with an integrated reporter for TLR-MCV1 reporter editing. HEK293T is an epithelial-like cell that was isolated from the kidney of a patient.

Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain

Experiment  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J  Last Updated Date: 2021-04-15
 
Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged.

Testing newly chemically modified crRNA and tracrRNA to activate the TLR reporter in human cells

Experiment  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J  Last Updated Date: 2021-04-15
 
Chemically modified crRNA and tracrRNA were delivered by electroporation in presence of amphiphilic peptide to transgenic human HEK-293T cells harboring the TLR-MCV1 reporter. Gene editing was determined by reporter activation.

Delivery of RNP containing chemically modified crRNA C20 with chemically modified tracrRNA T2-PS to determine the RNP distribution in TLR-MCV mouse brain

Experiment  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J  Last Updated Date: 2021-04-15
 
Chemically modified crRNA and tracrRNA were injected into the striatum of TLR-MCV mice and the distribution of RNP was imaged.

SpyCas9-3xNLS

Genome Editor  - [In Vivo, In Vitro] [Delivery Systems Initiative] [Human, Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
SpyCas9-3xNLS is type II-A Cas9 from Streptococcus pyogenes strain SF370. It was expressed from pMCSG7 bacterial expressing vector and purified from Escherichia coli Rosetta DE3 strain. SpyCas9 fused to 3 NLS: C-Myc-like NLS at the N-terminal SV40 NLS and Nucleoplasmin NLS at the C-terminal

SpyCas9-3xNLS

Genome Editor  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
SpyCas9-3xNLS is type II-A Cas9 from Streptococcus pyogenes strain SF370. It was expressed from pMCSG7 bacterial expressing vector and purified from Escherichia coli Rosetta DE3 strain. SpyCas9 fused to 3 NLS: C-Myc-like NLS at the N-terminal SV40 NLS and Nucleoplasmin NLS at the C-terminal
Show Experiments (5)

Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
Delivery of RNP containing chemically modified crRNA C20 with chemically modified tracrRNA T2-PS to determine the RNP distribution in TLR-MCV mouse brain
Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain
Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain

STS159 (mTmG; Sp_t2-PS:Sp_c0_mTmG)

Guide  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets mTmG and related reporter transgenes

STS159 (mTmG; Sp_t2:Sp_c20_mTmG)

Guide  - [In Vivo, In Vitro] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets mTmG and related reporter transgenes

STS159 (mTmG; Sp_t2:Sp_c20_mTmG)

Guide  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets mTmG and related reporter transgenes

STS96 (PCSK9a; Sp_t2:Sp_c20_PCSK9a)

Guide  - [In Vitro] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets endogenous mouse locus

Hepa1-6

Model System  - [In Vitro] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Stable mouse cell line of liver epithelial cells.

Neuro 2A

Model System  - [In Vitro] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Neuro-2a cells are mouse neuroblasts with neuronal and amoeboid stem cell morphology isolated from brain tissue.

RRID:AB_1196615 

Antibody  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Other Id: AB_1196615 
GFP (D5.1) XP Rabbit mAb antibody, Cell Signaling Technology

RRID:AB_2798820 

Antibody  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Other Id: AB_2798820 
Cas9 (S. pyogenes) (E7M1H) XPĀ® Rabbit mAb antibody, Cell Signaling Technology

Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells

Experiment  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B  Last Updated Date: 2020-11-02
 
Delivery of Cas9 RNP targeting human CFTR in Primary human epithelia cells. Gene editing efficiency was determined by percentage of NGS reads that showed an indel

Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells

Experiment  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
McCray Paul B  Last Updated Date: 2020-11-02
 
Delivery of Cas12a RNP targeting human CFTR in Primary human epithelia cells. Gene editing efficiency was determined by percentage of NGS reads that showed an indel

Testing AAV5 for activation of tdTomato in mouse airway club and ciliated cells

Experiment  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
Gao Guang-Ping  Last Updated Date: 2021-09-21
 
AAV2/5 mediated gene editing in the mouse airway was tested by deliverying SpCas9 and guide RNAs targeting the Ai9 transgene in Ai9 transgenic mice. Gene editing quantified by tdTomato activation and cell specific markers for club and ciliated cell types.

AsCas12a (IDT and Feldan Therapeutics)

Genome Editor  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides

GFP-NLS

Genome Editor  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Nuclear targeted GFP

FSD114

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD115

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD122

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD132

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD147

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD168

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD186

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD188

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD197

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD286

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD297

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD317

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD318

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD319

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD323

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD330

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD332

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD335

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD360

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD361

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD57d1

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD57d4

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD94

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

FSD95

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

S10-MOD

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

S10 Scr.

Delivery System  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
Shuttle peptide used to deliver reagents to airway epithelia

AAV.pU1a-SpCas9

Vector  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
Expresses codon-optimized SpCas9 in mammalian cells. HA-SV40NLS-SpCas9-SV40NLS

NKG2A

Guide  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides
gRNA targeting human NKG2A exon 3

Antibody  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Develop Combinatorial Non-Viral and Viral CRISPR Delivery for Lung Diseases
Other Id:   Cell Signaling Technology Cat# 2956 
GFP (D5.1) XP Rabbit mAb antibody

Ai14 mouse (congenic)

Model System  - [In Vivo] [Small Animal Testing Center (SATC), Delivery Systems Initiative] [Mouse]
Matched on:  -->  Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to  Repeat experiment of independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9  Independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain.

SpyCas9 g-loxP2_C9

Guide  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Independent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia
This sgRNA targets the mTmG transgene

Heaney-SATC_McCray-Validation_Lung Inflation and Fixation Protocol

Protocol  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Independent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia
Procedure for mouse lung inflation and fixation.

AB_2532994 

Antibody  - [In Vivo] [Small Animal Testing Center (SATC)] [Mouse]
Matched on:  -->  Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to
Other Id: 13-0300Ā  
Thermo-Fisher, Rat anti-GFAP

Testing newly chemically modified crRNA and tracrRNA in mTmG mouse embryonic fibroblasts

Experiment  - [In Vitro] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J  Last Updated Date: 2021-04-15
 
Chemically modified crRNA and tracrRNA were delivered by electroporation to embryonic fibroblasts harvested from the mTmG reporter mouse. Gene editing was determined by reporter activation.

Testing newly chemically modified crRNA and tracrRNA in mouse Hepa 1-6 cells

Experiment  - [In Vitro] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J  Last Updated Date: 2021-04-15
 
Chemically modified crRNA and tracrRNA were delivered by electroporation to mouse Hepa 1-6 cells. Editing activity was determined by Sanger sequencing

Testing newly chemically modified crRNA and tracrRNA to activate the TLR1 reporter in human cells

Experiment  - [In Vitro] [Delivery Systems Initiative] [Human]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Sontheimer Erik J  Last Updated Date: 2021-04-15
 
Chemically modified crRNA and tracrRNA were delivered by electroporation to transgenic human HEK-293T cells harboring the TLR1 reporter. Gene editing was determined by reporter activation.

STS205 (BACE1; Sp_t41:Sp_c20_Bace1)

Guide  - [In Vitro] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Targets endogenous mouse locus

TLR-MCV1 mouse

Model System  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
TLR-MCV1 transgene knocked into Rosa26 locus

RRID:AB_2536526 

Antibody  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
Other Id: AB_2536526 
GFP Recombinant Rabbit Monoclonal Antibody, Thermo Fisher Scientific #G10362

CasĪ¦-2

Genome Editor  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Compact editor of the Cas12j family identified in biggie phage from metagenomic assemblies

Cas12j-GFP10 (CasĪ¦-3)

Guide  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasĪ¦-3

Cas12j-GFP11 (CasĪ¦-3)

Guide  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasĪ¦-3

Cas12j-GFP13 (CasĪ¦-3)

Guide  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasĪ¦-3

Cas12j-GFP15 (CasĪ¦-1)

Guide  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasĪ¦-1

Cas12j-GFP1 (CasĪ¦-1)

Guide  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasĪ¦-1

Cas12j-GFP7 (CasĪ¦-2)

Guide  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasĪ¦-2

Cas12j-GFP8 (CasĪ¦-2)

Guide  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasĪ¦-2

Cas12j-GFP9 (CasĪ¦-2)

Guide  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasĪ¦-2

SapI-GG stuffer (CasĪ¦-2)

Guide  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Non-targeting guide RNA targeting compatible with CasĪ¦-2

SapI-GG stuffer (CasĪ¦-3)

Guide  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Non-targeting guide RNA targeting compatible with CasĪ¦-3

CasĪ¦-1

Genome Editor  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Compact editor of the Cas12j family identified in biggie phage from metagenomic assemblies

Cas12j-GFP14 (CasĪ¦-2)

Guide  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasĪ¦-2

Cas12j-GFP14 (CasĪ¦-3)

Guide  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasĪ¦-3

Cas12j-GFP3 (CasĪ¦-2)

Guide  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasĪ¦-2

Cas12j-GFP5 (CasĪ¦-3)

Guide  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasĪ¦-3

Cas12j-GFP6 (CasĪ¦-2)

Guide  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasĪ¦-2

Cas12j-GFP6 (CasĪ¦-3)

Guide  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasĪ¦-3

Cas12j-GFP7 (CasĪ¦-3)

Guide  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasĪ¦-3

AAV9-CMV-SaCas9

Vector  - [In Vivo] [Delivery Systems Initiative] [Mouse]
Matched on:  -->  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to  Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to  Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9
AAV serotype 9 delivering CMV driven SaCas9

CasĪ¦-3

Genome Editor  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Compact editor of the Cas12j family identified in biggie phage from metagenomic assemblies

pPP441

Vector  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Plasmid containing Homo sapiens codon optimized CasΦ-2 and spacer for editing in human cells.

pPP444

Vector  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Plasmid containing Homo sapiens codon optimized CasΦ-3 and spacer for editing in human cells.

Cas12j-GFP10 (CasĪ¦-2)

Guide  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasĪ¦-2

Cas12j-GFP12 (CasĪ¦-3)

Guide  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasĪ¦-3

Cas12j-GFP1 (CasĪ¦-2)

Guide  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasĪ¦-2

Cas12j-GFP21 (CasĪ¦-1)

Guide  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasĪ¦-1

Cas12j-GFP23 (CasĪ¦-1)

Guide  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasĪ¦-1

Cas12j-GFP9 (CasĪ¦-3)

Guide  - [In Vitro] [Genome Editors] [Human]
Matched on:  -->  Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems
Guide targeting eGFP compatible with CasĪ¦-3