125 results for Epithelium
125 results for Epithelium
Testing Shuttle Peptides ability to deliver GFP-NLS to airway epithelia. - Experiment [Delivery Systems Initiative] - [In Vivo] |
Alt-R® S.p. Cas9 Nuclease V3 - Genome Editor [Delivery Systems Initiative] |
g-loxP2_C9 - Guide [Delivery Systems Initiative] - [In Vivo] |
BALB/c mouse - Model System [Delivery Systems Initiative] - [In Vivo] |
Anti-CC10 (Rabbit) Polyclonal Antibody, dilution used 1:2,000 |
AsCas12a (IDT and Feldan Therapeutics) - Genome Editor [Delivery Systems Initiative] |
Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia - Experiment [Delivery Systems Initiative] - [In Vivo] |
mTmG mouse - Model System [Delivery Systems Initiative] - [In Vivo] |
Cre recombinase - Genome Editor [Small Animal Testing Center (SATC), Delivery Systems Initiative]
|
ssAAV5-sgB.saCas9 - Vector [Small Animal Testing Center (SATC)] |
BCM_ssAAV5-Sa_sgB - Guide [Small Animal Testing Center (SATC)] - [In Vivo] |
Anti-RFP (RABBIT) Antibody |
Anti-RFP (Mouse) Monoclonal Antibody, dilution used 1:300 |
Anti-RFP (Rabbit) Polyclonal Antibody |
Anti-alpha Tubulin (Mouse) Monoclonal Antibody, dilution used 1:200 |
AAV.pU1a-SpCas9 - Vector |
AAV2/5 expressing SpyCas9. AAV2/5 expressing two sgRNAs under U6 promoter and eGFP
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g-loxPbot_C12a - Guide [Delivery Systems Initiative] - [In Vivo] |
SaCas9 - Genome Editor [Small Animal Testing Center (SATC)] |
S10 - Delivery System [Delivery Systems Initiative] - [In Vivo, In Vitro]
|
scAAV5-Cre-GFP - Vector [Small Animal Testing Center (SATC)] |
GFP (D5.1) XP Rabbit mAb antibody |
D10 - Delivery System [Small Animal Testing Center (SATC)] - [In Vivo] |
Ai9 mouse - Model System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo]
|
D237 - Delivery System [Small Animal Testing Center (SATC)] - [In Vivo] |
SpCas9 - Genome Editor [Small Animal Testing Center (SATC)] |
BCM_ssAAV5-Sp_sgB - Guide [Small Animal Testing Center (SATC)] - [In Vivo] |
SpyCas9 g-loxP2_C9 - Guide [Small Animal Testing Center (SATC)] - [In Vivo] |
ssAAV5-sgA+sgB-U1A.GFP - Vector [Small Animal Testing Center (SATC)] |
Ai9 mouse (BCM) - Model System [Small Animal Testing Center (SATC)] - [In Vivo] |
Ai9-SauSpyCas9 mouse - Model System [Small Animal Testing Center (SATC)] - [In Vivo] |
[Validation] Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides - Experiment [Small Animal Testing Center (SATC)] - [In Vivo] |
ssAAV5-spCas9 - Vector [Small Animal Testing Center (SATC)] |
BCM_ssAAV5-Sp_sgA - Guide [Small Animal Testing Center (SATC)] - [In Vivo] |
mTmG mouse (congenic) - Model System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo]
|
[Validation] Validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice - Experiment [Small Animal Testing Center (SATC)] - [In Vivo] |
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector. - Experiment [Delivery Systems Initiative] - [In Vivo] |
SaCas9-Lagor - Genome Editor [Delivery Systems Initiative]
|
AAV9-Ai9-sgRNA1 + sgRNA2 - Vector [Delivery Systems Initiative] |
AAVcc47_pTR_self comp 2xU6-Ai9 guides - Vector [Delivery Systems Initiative] |
113-O12B - Delivery System [Delivery Systems Initiative] - [In Vivo] |
Testing virus region 4 (VR4) mutant cross-species compatible Adeno Associated Viruses (ccAAVs) in mice. - Experiment [Delivery Systems Initiative] - [In Vivo] |
[Validation] Validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing. - Experiment [Small Animal Testing Center (SATC)] - [In Vivo] |
SauCas9 - Genome Editor [Small Animal Testing Center (SATC)] |
Ai9 SaCas9 Guide B - Guide [Small Animal Testing Center (SATC)] - [In Vivo] |
STS159 (mTmG; Sp_t2:Sp_c20_mTmG) - Guide [Delivery Systems Initiative] - [In Vivo, In Vitro] |
RNP-NC-RVG - Delivery System [Small Animal Testing Center (SATC)] - [In Vivo] |
Other Id:
A21202
Invitrogen, Donkey anti-mouse alexafluor 488 |
CleanCap® Cas9 mRNA (5moU) - Genome Editor [Small Animal Testing Center (SATC)] |
sNLS-SpCas9-sNLS - Genome Editor [Small Animal Testing Center (SATC), Delivery Systems Initiative] |
RNP-NC-CPP - Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo] |
L2-modified - Guide [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo, In Vitro] |
BI28:AAV-GFAP-SaCas9-WPRE3-pA - Vector [Small Animal Testing Center (SATC), Delivery Systems Initiative] |
Other Id:
sc-17320
Sox-2 (Y-17) Antibody, Santa Cruz Biotechnology |
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter) - Experiment [Delivery Systems Initiative] - [In Vivo] |
AAV9_pTR_self comp 2xU6-Ai9 guides - Vector [Delivery Systems Initiative] |
AAVcc47-Ai9-sgRNA1-CB-SaCas9 - Vector [Delivery Systems Initiative] |
AAV9-Ai9-sgRNA2-CB-SaCas9 - Vector [Delivery Systems Initiative] |
SpyCas9-3xNLS - Genome Editor [Delivery Systems Initiative]
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Ai14 mouse - Model System [Delivery Systems Initiative] - [In Vivo] |
306-O12B - Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo, In Vitro]
|
Testing virus region 8 (VR8) mutant cross-species compatible Adeno Associated Viruses (ccAAVs) in mice. - Experiment [Delivery Systems Initiative] - [In Vivo] |
AAV9-GFP - Vector [Delivery Systems Initiative] |
C57BL/6 mouse (Asokan study) - Model System [Delivery Systems Initiative] - [In Vivo] |
Other Id:
ab150155
Abcam, Donkey anti-rat alexa fluour 647 |
AAVcc47-SaCas9-Ai9 - Vector [Small Animal Testing Center (SATC)] |
[Validation] Repeat experiment of second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear - Experiment [Small Animal Testing Center (SATC)] - [In Vivo] |
sg298 - Guide [Small Animal Testing Center (SATC)] - [In Vivo] |
[Validation] Second site validation of Deverman delivery platform using engineered AAVs to deliver CRSIPR/Cas9 to mouse brain - Experiment [Small Animal Testing Center (SATC)] - [In Vivo] |
R2-modified - Guide [Delivery Systems Initiative] - [In Vivo, In Vitro] |
Other Id:
25-6790
Anti-Myosin VIIa antibody, Proteus Biosciences |
RNP-NC-no ligand - Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo] |
Ai14 gRNA - Guide [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo] |
Testing new LNPs (lipid nanoparticles) for delivery of Fluc mRNA in adult mice - Experiment [Delivery Systems Initiative] - [In Vivo] |
Testing gRNA sequence and gRNA scaffold modified in Ai9 mice. - Experiment [Delivery Systems Initiative] - [In Vivo] |
BI28:AAV-GFAP-NLS-GFP-WPRE-synpA-L1-R2 - Vector [Small Animal Testing Center (SATC), Delivery Systems Initiative] |
L1-modified - Guide [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo, In Vitro] |
Cre Recombinase dose escalation study in Ai9 mice - Experiment [Delivery Systems Initiative] - [In Vivo] |
Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea - Experiment [Delivery Systems Initiative] - [In Vivo] |
AAV9-mCherry - Vector [Delivery Systems Initiative] |
AAVcc47-mCherry - Vector [Delivery Systems Initiative] |
AAVcc84-GFP - Vector [Delivery Systems Initiative] |
Testing preparation for validation at The Jackson Laboratory Small Animal Testing Center - Experiment [Delivery Systems Initiative] - [In Vivo] |
[Validation] Second site validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain - Experiment [Small Animal Testing Center (SATC)] - [In Vivo] |
Other Id:
13-0300Â
Thermo-Fisher, Rat anti-GFAP |
Other Id:
A21207
Invitrogen, Donkey anti-rabbit alexa fluor 594 |
CleanCap® Fluc - Genome Editor [Delivery Systems Initiative] |
306-O12B blank - Delivery System [Small Animal Testing Center (SATC)] - [In Vivo] |
Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) - Experiment [Delivery Systems Initiative] - [In Vivo] |
Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter) - Experiment [Delivery Systems Initiative] - [In Vivo] |
AAV9-Ai9-sgRNA1-CB-SaCas9 - Vector [Delivery Systems Initiative] |
AAV9-CMV-SaCas9 - Vector [Delivery Systems Initiative]
|
AAVcc47-Ai9-sgRNA1 + sgRNA2 - Vector [Delivery Systems Initiative] |
AAVcc47-Ai9-sgRNA2-CB-SaCas9 - Vector [Delivery Systems Initiative] |
SaCas9 - Genome Editor [Small Animal Testing Center (SATC), Delivery Systems Initiative] |
AAVcc81-GFP - Vector [Delivery Systems Initiative] |
306-S10 - Delivery System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo, In Vitro]
|
Ai9 SaCas9 Guide A - Guide [Small Animal Testing Center (SATC)] - [In Vivo] |
CleanCap® Cas9 - Genome Editor [Delivery Systems Initiative] |
STS204 (DNMT1; Sp_t2:Sp_c20_Dnmt1) - Guide [Delivery Systems Initiative] - [In Vivo] |
Other Id:
AB_1196615
GFP (D5.1) XP Rabbit mAb antibody, Cell Signaling Technology
|
Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain. - Experiment [Delivery Systems Initiative] - [In Vivo] |
Other Id:
Rockland Cat# 600-401-379
Anti-RFP (RABBIT) Antibody |
Other Id:
ab104224Â
Abcam, mouse anti-NeuN |
Chicken Polyclonal anti-NeuN antibody |
[Validation] Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear - Experiment [Small Animal Testing Center (SATC)] - [In Vivo] |
sg298 - Guide [Delivery Systems Initiative] - [In Vivo, In Vitro] |
Ai14 mouse (congenic) - Model System [Small Animal Testing Center (SATC), Delivery Systems Initiative] - [In Vivo]
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125 results for Epithelium
Category | Type | Name | Description | View Associated.. |
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Experiment | In Vivo | Testing Shuttle Peptides ability to deliver GFP-NLS to airway epithelia. | Delivery of GFP via shuttle peptides to mouse airway epithelium via nasal instilation. Delivery efficiency was quantified in large and small airways by counting the number of GFP positive cells divided by the number of DAPI cells. | |
Genome Editor | Cas Nuclease | Alt-R® S.p. Cas9 Nuclease V3 | ||
Guide | g-loxP2_C9 | This sgRNA targets the Ai9 and related transgenes | ||
Model System | Animal | BALB/c mouse | BALB/cJ is a commonly used inbred. Key traits include a susceptibility to developing the demyelinating disease upon infection with Theiler's murine encephalomyelitis virus. The BALB/cJ substrain is susceptible to Listeria, all species of Leishmania, and several species of Trypanosoma, but is resistant to experimental allergic orchitis (EAO). | |
Antibody | Anti-CC10 (Rabbit) Polyclonal Antibody, dilution used 1:2,000 | |||
Genome Editor | Cas Nuclease | AsCas12a (IDT and Feldan Therapeutics) | ||
Experiment | In Vivo | Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia | In vivo shuttle peptide delivery of Cas9 and Cas12a RNPs in mouse airway epithelia. Gene editing was quantified by the GFP+ cells in large and small airways following 1 delivery of GFP protein by GFP positive cells compared to DAPI stained cells. | |
Genome Editor | Reporter | GFP-NLS | Nuclear targeted GFP | |
Guide | sgAi9L | This sgRNA targets the Ai9 and related transgenes | ||
Guide | sgAi9R | This sgRNA targets the Ai9 and related transgenes | ||
Model System | Animal | mTmG mouse | ROSAmT/mGÂ is a cell membrane-targeted, two-color fluorescent Cre-reporter allele. Prior to Cre recombination, cell membrane-localized tdTomato (mT) fluorescence expression is widespread in cells/tissues. Cre recombinase expressing cells (and future cell lineages derived from these cells) have cell membrane-localized EGFP (mG) fluorescence expression replacing the red fluorescence | |
Genome Editor | Recombinase | Cre recombinase | Cre recombinase delivered by AAV (see vector details) |
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Vector | Viral Vector | ssAAV5-sgB.saCas9 | ||
Guide | BCM_ssAAV5-Sa_sgB | This gRNA targets the Ai9 and related transgenes | ||
Antibody | Anti-RFP (RABBIT) Antibody | |||
Antibody | Anti-RFP (Mouse) Monoclonal Antibody, dilution used 1:300 | |||
Antibody | Anti-RFP (Rabbit) Polyclonal Antibody | |||
Antibody | Anti-alpha Tubulin (Mouse) Monoclonal Antibody, dilution used 1:200 | |||
Genome Editor | Cas Nuclease | SpCas9 | HA-SV40NLS-SpCas9-SV40NLS | |
Vector | Viral Vector | AAV.pU1a-SpCas9 | Expresses codon-optimized SpCas9 in mammalian cells. HA-SV40NLS-SpCas9-SV40NLS | |
Vector | Viral Vector | H509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1) | AAV2/5 expressing SpyCas9. AAV2/5 expressing two sgRNAs under U6 promoter and eGFP | |
Guide | g-loxPbot_C12a | This sgRNA targets the Ai9 and related transgenes at two sites | ||
Genome Editor | Cas Nuclease | SaCas9 | ||
Delivery System | Amphiphilic peptide | S10 | Shuttle peptide used to deliver reagents to airway epithelia |
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Vector | Viral Vector | scAAV5-Cre-GFP | ||
Antibody | GFP (D5.1) XP Rabbit mAb antibody | |||
Delivery System | Amphiphilic peptide | D10 | ||
Model System | Animal | Ai9 mouse | Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. |
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Delivery System | Amphiphilic peptide | D237 | ||
Genome Editor | Cas Nuclease | SpCas9 | ||
Guide | BCM_ssAAV5-Sp_sgB | This sgRNA targets the Ai9 and related transgenes | ||
Guide | SpyCas9 g-loxP2_C9 | This sgRNA targets the mTmG transgene | ||
Vector | Viral Vector | ssAAV5-sgA+sgB-U1A.GFP | ||
Model System | Animal | Ai9 mouse (BCM) | Ai9 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. | |
Model System | Animal | Ai9-SauSpyCas9 mouse | Ai9-SauSpyCas9 is a version of Ai9 that has a single guide RNA sites on both sides of the STOP cassette. This is a Cre reporter allele that has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. Ai9 mice express robust tdTomato fluorescence following Cre-mediated recombination. | |
Experiment | In Vivo | Validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides | Ribonucleoproteins for CRISPR/Cas9 editing are complexed with amphiphilic peptides for delivery to lung airway epithilia via intranasal instillation into mTmG reporter mice. Editing is detected by production of GFP protein, and green fluorescence in airway linings | |
Vector | Viral Vector | ssAAV5-spCas9 | ||
Guide | BCM_ssAAV5-Sp_sgA | This sgRNA targets the Ai9 and related transgenes | ||
Model System | Animal | mTmG mouse (congenic) | mTmG is a double-fluorescent reporter transgenic mouse which expresses membrane-targeted tdTomato flanked by loxP sequences, followed by membrane-targeted GFP. After genomic cleavage by Cas9 at two sites, or Cre recombinase between loxP sites, tdTomato expression is lost and GFP is expressed. |
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Experiment | In Vivo | Validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice | AAV5 encoding CRISPR/Cas editing machinery were delivered to the lungs of reporter mice by intratracheal instillation. After 4 weeks incubation, the mice were dissected and the lungs imaged for the presence of tdTomato fluorescence, indicating successful editing. Editing calculated by dividing the number of tdTomato+ red cells by the number of nuclei in each airway | |
Delivery System | Viral vector | AAV | See vector details | |
Experiment | In Vivo | Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector. | A dual vector strategy was employed: one self complementary vector delivering two single guide RNAs targeting the Rosa26 locus and one delivering CMV driven SaCas9 (single stranded vector). This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=4) by intravenous injection in Ai9 mice. A total dose of 4e12vg was injected into each mouse and vectors mixed in a 1:1 ratio of cas9 to guide RNA (2e12vg of CMV Sacas9 vector and 2e12vg of the self complementary sgRNA vector) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart. | |
Genome Editor | Cas Nuclease | SaCas9-Lagor |
|
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Vector | Viral Vector | AAV9-Ai9-sgRNA1 + sgRNA2 | AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 targeting the Ai9 locus | |
Vector | Viral Vector | AAVcc47_pTR_self comp 2xU6-Ai9 guides | AAVcc47 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 transgene | |
Delivery System | Nanoparticle | 113-O12B | combinatorial library cationic lipid nanoparticles | |
Experiment | In Vivo | Testing virus region 4 (VR4) mutant cross-species compatible Adeno Associated Viruses (ccAAVs) in mice. | C57/BL6 mice (N=3) were injected intravenously at a dose of 5e13 vg/kg per mouse with a self-complementary AAV9 or ccAAV vector encoding an mCherry reporter. The biodistribution of of virus transduction was chacterized in various tissues and cell types by fluorescence imaging quantification. | |
Experiment | In Vivo | Validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing. | Quantification of CRISPR/Cas editing in liver and heart following custom AAV-mediated delivery. Detection of editing in non-target tissues. | |
Genome Editor | Cas Nuclease | SauCas9 | ||
Guide | Ai9 SaCas9 Guide B | This gRNA targets the Ai9 and related transgenes | ||
Guide | STS159 (mTmG; Sp_t2:Sp_c20_mTmG) | Targets mTmG and related reporter transgenes | ||
Delivery System | Nanoparticle | RNP-NC-RVG | The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a RVG peptide YTIWMPENPRPGTPCDIFTNSRGKRASNG which specifically interacts withthe N-acetylecholine receptor (AchR) on neuronal cells, which mediates NP entry | |
Antibody | AB_141607 | Invitrogen, Donkey anti-mouse alexafluor 488 | ||
Genome Editor | Cas Nuclease | CleanCap® Cas9 mRNA (5moU) | SpCas9 mRNA with 2 NLS signals, HA tag and capped using CleanCap. It is polyadenylated, substituted with a modified uridine and optimized for mammalian systems. It mimics a fully processed mature mRNA. | |
Genome Editor | Cas Nuclease | sNLS-SpCas9-sNLS | SpCas9 with N- and C-terminal SV40 NLS | |
Delivery System | Nanoparticle | RNP-NC-CPP | The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a cell penetrating peptide (CPP) from the TAT peptide (GRKKRRQRRRPQ) which lacks cell-type specficity | |
Guide | L2-modified | This gRNA targets the Ai9 and related transgenes | ||
Vector | Viral Vector | BI28:AAV-GFAP-SaCas9-WPRE3-pA | Novel engineered AAV BI28 variant expressing S. aureus Cas9 driven by the glial fibrillary acidic protein (GFAP) promoter | |
Antibody | AB_2286684 | Sox-2 (Y-17) Antibody, Santa Cruz Biotechnology | ||
Experiment | In Vivo | Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter) | A dual vector strategy was employed: one delivering a single guide RNA and CB driven SaCas9, and another delivering the second guide RNA and CB driven SaCas9. This strategy was evaluted with both AAV9 (n=4) and AAVcc47 (n=5) by intravenous injection in Ai9 mice. A total dose of 2e12vg was injected into each mouse (1e12vg each vector mixed 1:1) and organs were harvested 4 weeks post injection. Editing efficency was determined by calculating percent TdTomato+ cells normalized to Dapi+ cells in liver and heart. | |
Vector | Viral Vector | AAV9_pTR_self comp 2xU6-Ai9 guides | AAV serotype 9 delivering u6 promoter driving sgRNA 1 + sgRNA2 (self complementray vector) targeting Ai9 transgene | |
Vector | Viral Vector | AAVcc47-Ai9-sgRNA1-CB-SaCas9 | AAVcc47 delivering sgRNA 1 + CB SaCas9 targeting the Ai9 locus | |
Vector | Viral Vector | AAV9-CMV-Cre | AAV serotype 9 delivering CMV Cre Recombinase | |
Vector | Viral Vector | AAV9-Ai9-sgRNA2-CB-SaCas9 | AAV serotype 9 delivering gRNA 2 + CB SaCas9 targeting the Ai9 locus | |
Vector | Viral Vector | AAVcc47-CMV-SaCas9 | AAVcc47 delivering CMV driven SaCas9 | |
Guide | sgAi9L | This sgRNA targets the Ai9 and related transgenes | ||
Genome Editor | Cas Nuclease | SpyCas9-3xNLS | SpyCas9-3xNLS is type II-A Cas9 from Streptococcus pyogenes strain SF370. It was expressed from pMCSG7 bacterial expressing vector and purified from Escherichia coli Rosetta DE3 strain. SpyCas9 fused to 3 NLS: C-Myc-like NLS at the N-terminal SV40 NLS and Nucleoplasmin NLS at the C-terminal |
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Model System | Animal | C57BL/6J mouse | C57BL/6J WT mouse | |
Vector | Viral Vector | AAVcc47-CMV-Cre | AAVcc47 delivering CMV Cre Recombinase | |
Model System | Animal | Ai14 mouse | Ai14 mouse has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. The att site flanked neo selection cassette has been removed in this strain. | |
Delivery System | Nanoparticle | 306-O12B | Combinatorial library cationic lipid nanoparticles |
|