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STS120 (TLR1) (3 pmol RNP) | SCGE Toolkit
Database of study and experiment data generated by the Somatic Cell Genome Editing Consortium
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Experiment Record Detail
Experiment
Testing newly chemically modified crRNA and tracrRNA to activate the TLR1 reporter in human cells
Experiment Condition
STS120 (TLR1) (3 pmol RNP)
Assay Description:
Calculated editing efficiency by flow cytometry analysis of RFP.
Tissue Measured:
Measurement Type:
Editing Efficiency
Record ID
15000000552
Editor
SpyCas9-3xNLS
Delivery System
Delivery System Subtype
Guide
STS120 (TLR1; Sp_t2:Sp_c20_TLR1)
Vector
Model Species
Human
Model Name
HEK-293T-disrupted_GFP-mcherry-Puro
Application Method
electroporation
Application Site
Dosage
3 pmol RNP
Injection Frequency
Injection Rate
Injection Volume
Days post injection
48-72 hours
Editor Format
protein, RNA
Antidote Id
Antidote Description
Measured Values
Samples Size:
3
Replicate
Result (Editing Efficiency)
Measurement Units:
% of RFP+ cells
Mean
25.0 
1
22 
2
26 
3
27