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ExperimentComparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.
Experiment ConditionMock
Assay Description: Editing efficiency calculated by counting fluorescent cells (TdTomato) compared to DAPI stained cells.
Tissue Measured:heart
Measurement Type:Editing Efficiency
Record ID15000001041

Editor
Delivery System
Delivery System Subtype
Guide
Vector

Model SpeciesMouse
Model NameB6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J

Application Methodintravenous
Application Siteliver and heart
Dosage
Injection Frequency
Injection Rate
Injection Volume
Days post injection4 weeks
Editor Format
Antidote Id
Antidote Description

Measured Values
Samples Size: 1
 
Replicate Result (Editing Efficiency)
Measurement Units: % of cells
Mean 0.04 
1 0.043473