Development System AAV2/2, No Flow on Chip | SCGE Toolkit Database of study and experiment data generated by the Somatic Cell Genome Editing Consortium
AAV tropism in kidney organoids cultured under flow
AAV2/2, No Flow on Chip
Calculated total transduction efficiency by counting fluorescent cells (GFP) compared to DAPI stained cells.
Delivery Efficiency
15000003408
AAV
Viral vector
AAV2/2CMVeGFP ;
Human
Human kidney organoid (stem cell-derived)
Virus
MOI=10^5
7 days
AAV encoded
RRID:AB_300798
ab13970 (Abcam)
Chicken anti-GFP polyclonal antibody
RRID:AB_2336558
B-1325-2 (Vector Labs)
Lotus tetragonolobus lectin (LTL)
RRID:AB_354920
AF1658 (R&D Systems)
Goat anti-podocalyxin polyclonal antibody
Measured Values
Samples Size: 2
Mean
38.98 
1
61.238 
2
16.724 
Mean
28.39 
1
28.39044 
Mean
1.73 
1
1.727099