Development System AAV2/2, Low Flow on Chip | SCGE Toolkit Database of study and experiment data generated by the Somatic Cell Genome Editing Consortium
AAV tropism in kidney organoids cultured under flow
AAV2/2, Low Flow on Chip
Calculated total transduction efficiency by counting fluorescent cells (GFP) compared to DAPI stained cells.
Delivery Efficiency
15000003412
AAV
Viral vector
AAV2/2CMVeGFP ;
Human
Human kidney organoid (stem cell-derived)
Virus
MOI=10^5
7 days
AAV encoded
RRID:AB_300798
ab13970 (Abcam)
Chicken anti-GFP polyclonal antibody
RRID:AB_2336558
B-1325-2 (Vector Labs)
Lotus tetragonolobus lectin (LTL)
RRID:AB_354920
AF1658 (R&D Systems)
Goat anti-podocalyxin polyclonal antibody
Measured Values
Samples Size: 2
Mean
3.57 
1
0.931 
2
6.204 
Mean
3.85 
1
3.849576 
Mean
0.97 
1
0.970969