Experiment AAV2/2 transduction efficiencies in kidney organoids cultured under flow determined by FACS Experiment Condition Pulsed flow Assay Description: Calculated transduction efficiency by flow cytometry of GFP+ cells out of total cells Tissue Measured: Measurement Type: Delivery Efficiency Record ID 15000003421 Editor Delivery System AAV Delivery System Subtype Viral vector Guide Vector AAV2/2CMVeGFP; Model Species Human Model Name Human kidney organoid (iPSC-derived) Application Method Virus Application Site Dosage MOI=10^5 Injection Frequency Injection Rate Injection Volume Days post injection 8 days Editor Format AAV encoded Antidote Id Antidote Description Antibody RRID RRID:AB_300798 Antibody Other ID ab13970 (Abcam) Antibody Description Chicken anti-GFP polyclonal antibody Antibody RRID RRID:AB_2336558 Antibody Other ID B-1325-2 (Vector Labs) Antibody Description Lotus tetragonolobus lectin (LTL) Measured Values Samples Size: 1   Replicate Result (Delivery Efficiency) Measurement Units: % GFP+ LTL+ / LTL+ cells Mean 10.52  1 10.523640061006608  Measurement Units: %GFP+ / total cells Mean 6.59  1 6.59