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ExperimentAAV2/2 transduction efficiencies in kidney organoids cultured under flow determined by FACS
Experiment ConditionNo flow
Assay Description: Calculated transduction efficiency by flow cytometry of GFP+ cells out of total cells
Tissue Measured:
Measurement Type:Delivery Efficiency
Record ID15000003422

Editor
Delivery SystemAAV
Delivery System SubtypeViral vector
Guide
VectorAAV2/2CMVeGFP;

Model SpeciesHuman
Model NameHuman kidney organoid (iPSC-derived)

Application Methodvirus
Application Site
DosageMOI=10^5
Injection Frequency
Injection Rate
Injection Volume
Days post injection8 days
Editor FormatAAV vector encoded
Antidote Id
Antidote Description

Antibody RRIDRRID:AB_300798
Antibody Other IDab13970 (Abcam)
Antibody DescriptionChicken anti-GFP polyclonal antibody

Antibody RRIDRRID:AB_2336558
Antibody Other IDB-1325-2 (Vector Labs)
Antibody DescriptionLotus tetragonolobus lectin (LTL)

Measured Values
Samples Size: 1
 
Replicate Result (Delivery Efficiency)
Measurement Units: % GFP+ LTL+ / LTL+ cells
Mean 6.44 
1 6.436629064366291 
Measurement Units: %GFP+ / total cells
Mean 4.26 
1 4.26