AAV2/2 transduction efficiencies in kidney organoids cultured under flow determined by FACS |
No flow |
Calculated transduction efficiency by flow cytometry of GFP+ cells out of total cells |
|
Delivery Efficiency |
15000003422 |
|
|
AAV |
Viral vector |
|
AAV2/2CMVeGFP; |
|
Human |
Human kidney organoid (iPSC-derived) |
|
virus |
|
MOI=10^5 |
|
|
|
8 days |
AAV vector encoded |
|
|
|
RRID:AB_300798 |
ab13970 (Abcam) |
Chicken anti-GFP polyclonal antibody |
|
RRID:AB_2336558 |
B-1325-2 (Vector Labs) |
Lotus tetragonolobus lectin (LTL) |
|
Measured Values |
Samples Size: | 1 |
|
Mean |
6.44  |
1 |
6.436629064366291  |
Mean |
4.26  |
1 |
4.26  |