Experiment Evaluation of DNA damage, cellular toxicity and inflammatory markers following saCas9 and gRNAs delivery by AAV2 in kidney organoids. Experiment Condition AAV2/2-SaCas9 + AAV2/2-gRNA Assay Description: Compartmental DNA damage was calculated as the percent of YH2AX co-staining among LTL, CHD1 and MEIS1/2/3 positive cells Tissue Measured: Measurement Type: Biomarker Detection Record ID 15000003443 Editor SaCas9 (AAV-SaCas9) Delivery System AAV Delivery System Subtype Viral vector Guide DMD low gRNA-2 Vector AAV2-gRNA; Model Species Human Model Name Human kidney organoid (stem cell-derived) Application Method virus Application Site Dosage MOI=10^5 Injection Frequency Injection Rate Injection Volume Days post injection 7 days Editor Format dual AAV encoded Antidote Id Antidote Description Antibody RRID RRID:AB_2750570 Antibody Other ID Active Motif Cat# 39795 Antibody Description Mouse anti-meis1/2/3 Antibody RRID RRID:AB_2118010 Antibody Other ID Cell Signaling Technology Cat# 2577 Antibody Description Rabbit anti-Phospho-Histone H2A.X (Ser139) Antibody RRID RRID:AB_307284 Antibody Other ID Abcam Cat# ab9498 Antibody Description Mouse anti-CD31 antibody Antibody RRID RRID:AB_2336558 Antibody Other ID Vector Laboratories Cat# B-1325 Antibody Description Lotus tetragonolobus lectin (LTL) Antibody RRID RRID:AB_2116561 Antibody Other ID R and D Systems Cat# AF1750 Antibody Description Goat anti-KIM1/HAVCR Measured Values Samples Size: 3   Replicate Result (Biomarker Detection) Measurement Units: %KIM1+/LTL+ cells Mean 69.29  1 83.33333  2 66.66667  3 50  4 75  5 75  6 75  7 60  Measurement Units: %YH2AX+/CHD1+ cells Mean 55.07  1 43.3333333333333  2 77.4193548387097  3 44.4444444444444  Measurement Units: %YH2AX+/LTL+ cells Mean 89.34  1 87.1508379888268  2 94.2028985507246  3 86.6666666666667  Measurement Units: %YH2AX+/MEIS1/2/3+ cells Mean 34.87  1 29.375  2 33.6538461538462  3 41.5770609318996  Measurement Units: IL-10 (pg/mL) Mean 487.92  1 495.417375758954  2 464.933192143006  3 526.17876439772  4 476.437321338346  5 497.545747150637  6 453.317100769354  7 522.919777936497  8 466.579803799361  Measurement Units: IL-6 (pg/mL) Mean 6.2  1 7.13986096462846  2 7.51252056707063  3 3.84216984250873  4 5.13790801017772  5 6.91414028649866  6 5.20233895635352  7 6.79682820460547  8 7.06756386236927  Measurement Units: IL1-beta (pg/mL) Mean 3.05  1 3.04035320059101  2 3.45685924657181  3 2.50921630343338  4 2.51575347278385  5 3.02762559397732  6 2.63136714995803  7 3.56041744685856  8 3.69759523331727  Measurement Units: KIM-1 (pg/mL) Mean 101.69  1 73.0450520275434  2 247.799688076254  3 57.3500937297986  4 51.5853862679727  5 104.236737882331  6 50.1516171305266  7 102.742313385041  8 126.625898599655  Measurement Units: MCP-1 (pg/mL) Mean 10.69  1 10.552638994931  2 10.0996567940629  3 6.94973325618479  4 6.54733914189619  5 16.9785383180146  6 6.82945998412296  7 17.9601172079959  8 9.57669773080734  Measurement Units: NGAL (pg/mL) Mean 3682.05  1 2240.35305387958  2 3082.66840339688  3 2182.40694910355  4 2029.08363507414  5 5895.0685121659  6 2129.72429900216  7 6258.05943091146  8 5639.0439532151