Contact SCGE




SCGE Consortium Home   |  About SCGE Toolkit   |  Contact Us   |  Citing the Toolkit   |  License  
ExperimentTesting virus region 4 (VR4) mutant cross-species compatible Adeno Associated Viruses (ccAAVs) in mice.
Experiment ConditionAAV9-mCherry
Assay Description: Relative neuronal transduction levels were quantified by counting the number of transduced neurons, identified based on morphology, for each brain region per 50um sagittal section
Tissue Measured:striatum
Cell Type :astrocyte
Measurement Type:Delivery Efficiency
Record ID15000000356

Editor
Delivery System
Delivery System Subtype
Guide
VectorAAV9-mCherry;

Model SpeciesMouse
Model NameC57BL/6

Application Methodintravenous
Application Site
Dosage5e13 vg/kg
Injection Frequencyonce
Injection Rate
Injection Volume200 microliters
Days post injection4 weeks
Editor FormatAAV vector encoded
Antidote Id
Antidote Description

Measured Values
Samples Size: 3
 
Replicate Result (Delivery Efficiency)
Measurement Units: # of cells in 50um Section
Mean 46.83 
1 38 
2 37 
3 65.5 
  

Representative image of AAV9-mCherry delivery in brain striatum. Neuronal and astrocytic expression were visualized by immunohistochemical staining.


Publication Title
Cross-species evolution of a highly potent AAV variant for therapeutic gene transfer and genome editing. NCBI