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Filters ..
  Tissues
Renal/Urinary
  • Kidney
Liver and Biliary
  • Liver
Cardiovascular
  • Heart
Musculoskeletal
  • Skeletal muscle tissue
Nervous
  • Cerebral cortex (neuron)
  • Cerebral cortex (astrocyte)
  • Cerebellum (neuron)
  • Cerebellum (astrocyte)
  • Hippocampal formation (neuron)
  • Hippocampal formation (astrocyte)
  • Dorsal plus ventral thalamus (neuron)
  • Dorsal plus ventral thalamus (astrocyte)
  • Striatum (neuron)
  • Striatum (astrocyte)
  • Midbrain (neuron)
  • Midbrain (astrocyte)
  Cell Types
 astrocyte
 neuron
Models
 C57BL/6 mouse (Asokan study)
Sex
 Male
  Vectors
 AAV9-mCherry
 AAVcc47-mCherry
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Results

Testing_virus_region_4_(VR4)_mutant_cross-species_compatible_Adeno_Associated_Viruses_(ccAAVs)_in_mice._
Delivery Efficiency
Record Id Condition Tissue Cell Type Sex Model Vector Dosage Injection Frequency Delivery Efficiency # of cells in 50um Section Delivery Efficiency Corrected Total Fluorescence Image
15000000331 AAVcc47-mCherry cerebral cortex neuron Male C57BL/6 mouse (Asokan study) AAVcc47-mCherry 5e13 vg/kg 1X
15000000332 AAVcc47-mCherry cerebral cortex astrocyte Male C57BL/6 mouse (Asokan study) AAVcc47-mCherry 5e13 vg/kg 1X
15000000333 AAVcc47-mCherry cerebellum neuron Male C57BL/6 mouse (Asokan study) AAVcc47-mCherry 5e13 vg/kg 1X
15000000334 AAVcc47-mCherry cerebellum astrocyte Male C57BL/6 mouse (Asokan study) AAVcc47-mCherry 5e13 vg/kg 1X
15000000335 AAVcc47-mCherry hippocampal formation neuron Male C57BL/6 mouse (Asokan study) AAVcc47-mCherry 5e13 vg/kg 1X
15000000336 AAVcc47-mCherry hippocampal formation astrocyte Male C57BL/6 mouse (Asokan study) AAVcc47-mCherry 5e13 vg/kg 1X
15000000337 AAVcc47-mCherry dorsal plus ventral thalamus neuron Male C57BL/6 mouse (Asokan study) AAVcc47-mCherry 5e13 vg/kg 1X
15000000338 AAVcc47-mCherry dorsal plus ventral thalamus astrocyte Male C57BL/6 mouse (Asokan study) AAVcc47-mCherry 5e13 vg/kg 1X
15000000339 AAVcc47-mCherry striatum neuron Male C57BL/6 mouse (Asokan study) AAVcc47-mCherry 5e13 vg/kg 1X
15000000340 AAVcc47-mCherry striatum astrocyte Male C57BL/6 mouse (Asokan study) AAVcc47-mCherry 5e13 vg/kg 1X
15000000341 AAVcc47-mCherry midbrain neuron Male C57BL/6 mouse (Asokan study) AAVcc47-mCherry 5e13 vg/kg 1X
15000000342 AAVcc47-mCherry midbrain astrocyte Male C57BL/6 mouse (Asokan study) AAVcc47-mCherry 5e13 vg/kg 1X
15000000343 AAVcc47-mCherry kidney Male C57BL/6 mouse (Asokan study) AAVcc47-mCherry 5e13 vg/kg 1X
15000000344 AAVcc47-mCherry liver Male C57BL/6 mouse (Asokan study) AAVcc47-mCherry 5e13 vg/kg 1X
15000000345 AAVcc47-mCherry heart Male C57BL/6 mouse (Asokan study) AAVcc47-mCherry 5e13 vg/kg 1X
15000000346 AAVcc47-mCherry skeletal muscle tissue Male C57BL/6 mouse (Asokan study) AAVcc47-mCherry 5e13 vg/kg 1X
15000000347 AAV9-mCherry cerebral cortex neuron Male C57BL/6 mouse (Asokan study) AAV9-mCherry 5e13 vg/kg 1X
15000000348 AAV9-mCherry cerebral cortex astrocyte Male C57BL/6 mouse (Asokan study) AAV9-mCherry 5e13 vg/kg 1X
15000000349 AAV9-mCherry cerebellum neuron Male C57BL/6 mouse (Asokan study) AAV9-mCherry 5e13 vg/kg 1X
15000000350 AAV9-mCherry cerebellum astrocyte Male C57BL/6 mouse (Asokan study) AAV9-mCherry 5e13 vg/kg 1X
15000000351 AAV9-mCherry hippocampal formation neuron Male C57BL/6 mouse (Asokan study) AAV9-mCherry 5e13 vg/kg 1X
15000000352 AAV9-mCherry hippocampal formation astrocyte Male C57BL/6 mouse (Asokan study) AAV9-mCherry 5e13 vg/kg 1X
15000000353 AAV9-mCherry dorsal plus ventral thalamus neuron Male C57BL/6 mouse (Asokan study) AAV9-mCherry 5e13 vg/kg 1X
15000000354 AAV9-mCherry dorsal plus ventral thalamus astrocyte Male C57BL/6 mouse (Asokan study) AAV9-mCherry 5e13 vg/kg 1X
15000000355 AAV9-mCherry striatum neuron Male C57BL/6 mouse (Asokan study) AAV9-mCherry 5e13 vg/kg 1X
15000000356 AAV9-mCherry striatum astrocyte Male C57BL/6 mouse (Asokan study) AAV9-mCherry 5e13 vg/kg 1X
15000000357 AAV9-mCherry midbrain neuron Male C57BL/6 mouse (Asokan study) AAV9-mCherry 5e13 vg/kg 1X
15000000358 AAV9-mCherry midbrain astrocyte Male C57BL/6 mouse (Asokan study) AAV9-mCherry 5e13 vg/kg 1X
15000000359 AAV9-mCherry kidney Male C57BL/6 mouse (Asokan study) AAV9-mCherry 5e13 vg/kg 1X
15000000360 AAV9-mCherry liver Male C57BL/6 mouse (Asokan study) AAV9-mCherry 5e13 vg/kg 1X
15000000361 AAV9-mCherry heart Male C57BL/6 mouse (Asokan study) AAV9-mCherry 5e13 vg/kg 1X
15000000362 AAV9-mCherry skeletal muscle tissue Male C57BL/6 mouse (Asokan study) AAV9-mCherry 5e13 vg/kg 1X

Expression of VR4 ccAAVs in the brain following IV injection

Expression of VR4 ccAAVs in the brain. Adult C57/B6 mice were injected intravenously at 5e13 vg/kg (N=3). A self-complementary Mcherry cassette was packaged into lead ccAAV mutants driven by a chicken-beta actin human hybrid (CBh) promoter. Mice were sacrificed 4-weeks post injection brain was post-fixed in 4% PFA for 24 hrs. Tissue was cut into 50mm sagittal sections using a vibratome. Immunohistochemical staining of brain sections revealed neuronal and astrocytic expression (representative images are shown). 

Expression of VR4 ccAAVs in cardiac and skeletal muscle

Expression of VR4 ccAAVs in cardiac and skeletal muscle. Adult C57/B6 mice were injected intravenously at 5e13 vg/kg (N=3). A self-complementary Mcherry cassette was packaged into lead ccAAV mutants driven by a chicken-beta actin human hybrid (CBh) promoter. Mice were sacrificed 4-weeks post injection and heart and skeletal muscle was post-fixed in 4% PFA for 24 hrs. Fixed tissue was embedded in agarose and cut into 50mm thick sections using a vibratome. Native fluorescence was quantified using Image J. For each animal, 6 images were taken from 2 sections and used to calculate corrected total cell fluorescence using the formula: Integrated Density (Area of selected image X Mean fluorescence of background readings).

Expression of VR4 ccAAVs in liver and kidney

Expression of VR4 ccAAVs in liver and kidney. Adult C57/B6 mice were injected intravenously at 5e13 vg/kg (N=3). A self-complementary Mcherry cassette was packaged into lead ccAAV mutants driven by a chicken-beta actin human hybrid (CBh) promoter. Mice were sacrificed 4-weeks post injection and liver and kidney was post-fixed in 4% PFA for 24 hrs. Fixed tissue was embedded in agarose and cut into 50mm thick sections using a vibratome. Native fluorescence was quantified using Image J. For each animal, 6 images were taken from 2 sections and used to calculate corrected total cell fluorescence using the formula: Integrated Density (Area of selected image X Mean fluorescence of background readings).