Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells |
FSD199 |
Calculated editing efficiency by % of NGS reads that showed an indel |
|
Editing Efficiency |
15000000738 |
|
SpCas9 (Feldan Therapeutics) |
FSD199 |
Amphiphilic peptide |
McCray_CFTR_Cas9_Guide1 |
|
|
Human |
Primary Airway Epithelia |
|
|
|
2uM gRNA, 1.33uM Cas12a, 40uM Shuttle |
|
|
|
|
RNP |
|
|
|
Measured Values |
Samples Size: | 6 |
|
Mean |
5.15  |
1 |
5.78  |
2 |
9.92  |
3 |
9.26  |
4 |
1.61  |
5 |
1.56  |
6 |
2.76  |
| |
|