Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells |
FSD237 |
Calculated editing efficiency by % of NGS reads that showed an indel |
|
Editing Efficiency |
15000000747 |
|
SpCas9 (Feldan Therapeutics) |
FSD237 |
Amphiphilic peptide |
McCray_CFTR_Cas9_Guide1 |
|
|
Human |
Primary Airway Epithelia |
|
|
|
2uM gRNA, 1.33uM Cas12a, 40uM Shuttle |
|
|
|
|
RNP |
|
|
|
Measured Values |
Samples Size: | 17 |
|
Mean |
11.16  |
1 |
23.99  |
2 |
22.1  |
3 |
17.2  |
4 |
5.8  |
5 |
4.78  |
6 |
3.08  |
7 |
11.17  |
8 |
12.38  |
9 |
6.73  |
10 |
9.46  |
11 |
6.9  |
12 |
14.1050000190734  |
13 |
13.9600000381469  |
14 |
7.28999996185302  |
15 |
5.29  |
16 |
14.62  |
17 |
10.91  |
| |
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