Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells |
FSD321 |
Calculated editing efficiency by % of NGS reads that showed an indel |
|
Editing Efficiency |
15000000777 |
|
SpCas9 (Feldan Therapeutics) |
FSD321 |
Amphiphilic peptide |
McCray_CFTR_Cas9_Guide1 |
|
|
Human |
Primary Airway Epithelia |
|
|
|
2uM gRNA, 1.33uM Cas12a, 40uM Shuttle |
|
|
|
|
RNP |
|
|
|
Measured Values |
Samples Size: | 5 |
|
Mean |
8.71  |
1 |
15.34  |
2 |
6.81  |
3 |
2.69  |
4 |
13.82  |
5 |
4.9  |
| |
|