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ExperimentTesting newly chemically modified crRNA and tracrRNA in mouse Hepa 1-6 cells
Experiment ConditionSTS204 (DNMT1) (5 pmol RNP)
Assay Description: Calculated editing efficiency by PCR amplification and sanger sequencing analysis.
Tissue Measured:
Measurement Type:Editing Efficiency
Record ID15000000558

EditorSpyCas9-3xNLS
Delivery System
Delivery System Subtype
GuideSTS204 (DNMT1; Sp_t41:Sp_c20_Dnmt1)
Vector

Model SpeciesMouse
Model NameHepa1-6

Application Methodelectroporation
Application Site
Dosage5 pmol RNP
Injection Frequency
Injection Rate
Injection Volume
Days post injection72 hours
Editor Formatprotein, RNA
Antidote Id
Antidote Description

Measured Values
Samples Size: 1
 
Replicate Result (Editing Efficiency)
Measurement Units: % of Lesion
Mean 57.5 
1 57.5