Summary

SCGE ID10000000213
Lab IDSTS204 (DNMT1; Sp_t41:Sp_c20_Dnmt1)
Sourcein house production
Target LocusDnmt1
Speciesmouse
DescriptionTargets endogenous mouse locus
Guide CompatibilitySpyCas9
Target SequenceCGGGCTGGAGCTGTTCGCGC
Target Sequence + PAMCGGGCTGGAGCTGTTCGCGCTGG
PositionGRCm39/mm39/chr9:20818502-20864275
Full Guide Sequence

Sp_t41: AAUCGAAGCAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUAUGCA

Sp_c20_Dnmt1: CGGGCUGGAGCUGUUCGCGCGUUUUAGAGCUAUGCU

Spacer SequenceCGGGCUGGAGCUGUUCGCGC
Spacer Length20
Modifications

Sp_t41: (mA)#(mG)#(mC)#(mA)(mU)(mA)(mG)(mC)(mA)(mA)(mG)(rU)(rU)(mA)(rA)(mA)(mA)(rU)(mA)(mA)(mG)(mG)(rC)(rU)(mA)(rG)(rU)#(rC)(mC)(rG)(rU)(rU)(mA)(mU)(mC)(mA)(mA)(mC)(mU)(mU)(mG)(mA)(mA)(mA)(mA)(mA)(mG)(mU)(mG)(mG)(mC)(mA)(mC)(mC)(mG)(mA)(mG)(mU)(mC)(mG)(mG)(mU)(mG)(mC)#(mU)#(mU)#(mU)

Sp_c20_Dnmt1: (mC)#(mG)#(mG)#(mG)(mC)(mU)(mG)(mG)(mA)(mG)(fC)(fU)(fG)(fU)(rU)#(rC)#(fG)(fC)(rG)#(mC)(mG)(rU)#(rU)#(rU)#(fU)(fA)(mG)(mA)(mG)(mC)(mU)(mA)(mU)#(mG)#(mC)#(mU)

Repeat SequenceGUUUUAGAGCUAUGCU
Anit-Repeat SequenceAGCAUAGCAAGUUAAAAU
Stemloop 1 SequenceAAGGCUAGUCCGU
Stemloop 2 SequenceAACUUGAAAAAGUG
Stemloop 3 SequenceGCACCGAGUCGGUGC

Other guides that target The same sequence

  • STS204 (DNMT1; Sp_t2:Sp_c20_Dnmt1)
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    Related Editors

  • SpyCas9-3xNLS

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    Projects & Experiments

    This Guide: STS204 (DNMT1; Sp_t41:Sp_c20_Dnmt1) is being used ...
    Project Initiative Contact PI
    Delivery Systems Initiative Erik J Sontheimer, PhD
    (University of Massachusetts Medical School, Worcester)    		 NIH Report