Experiment Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea Experiment Condition 306-O12B Assay Description: Editing is displayed by tdTomato signal 48 hr after transfection. Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000000589 Editor CleanCap® Cas9 Delivery System 306-O12B Delivery System Subtype Nanoparticle Guide sg298 Vector Model Species Mouse Model Name Ai14 Primary Fibroblasts Application Method chemical Application Site Dosage LNP/Cas9/sgRNA=15/1/1, [LNP]=7.6ug/ml Injection Frequency Injection Rate Injection Volume Days post injection 48 hours Editor Format mRNA Antidote Id Antidote Description Measured Values Samples Size: 1   Replicate Result (Editing Efficiency) Measurement Units: Signal Mean 1 of 1 Present  1 Present