Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter) |
AAV9, Female |
Editing efficiency calculated by counting fluorescent cells (TdTomato) compared to DAPI stained cells. |
heart |
Editing Efficiency |
15000001026 |
|
SaCas9-Lagor |
|
|
|
AAV9-Ai9-sgRNA1 + sgRNA2; AAV9-CMV-SaCas9; |
|
Mouse |
B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J |
|
intravenous |
liver and heart |
1e12vg of CMV Sacas9 vector and 3e12vg of the sgRNA vector |
once |
|
200uL |
4 weeks |
SaCas9 protein and sgRNAs expressed from AAV |
|
|
|
Measured Values |
Samples Size: | 2 |
|
Mean |
2.57  |
1 |
2.85370869381591  |
2 |
2.27645410254654  |